2. www.cdr.fi
Unique Molecular Imaging Capabilities
5/23/2013 2Faculty of Pharmacy/ SPECT-CT/ CDR
Therapeutic areas
Oncology
CNS
Drug delivery
Cardiovascular
Inflammation
Early-Toxicology
Single Photon Emission Computed Tomography (SPECT)
NanoSPECT/CT
Functional & anatomical
imager
3. 5/23/2013 3Faculty of Pharmacy/ Name / CDR
We provide the instruments and expertise for real-time
visualization of molecules in vivo in small animals
SPECT/CT non-clinical imaging studies
Project specific services: Medical physics, radiopharmacy, biology
Consultation and training on molecular imaging in drug development
Collaborations are encouraged
Small Animal Molecular Imaging Unit
Services:
Facility:
SPECT/CT (NanoSPECT/CT with 4 heads, Bioscan Inc.).
Isotope laboratory with authorization to carry out animal
research including viral vectors (Biosafety level 2 laboratory).
Funding bodies:
5. www.cdr.fi
Biodistribution of [99mTc]-labelled
Stealth Liposomes in Tumor-Bearing
Mice
5/23/2013 5Faculty of Pharmacy/ SPECT-CT/ CDR
s.c. model i.p. model free label
Lehtinen J, Raki M, Bergström KA, Uutela P, et al. (2012) Pre-Targeting and Direct Immunotargeting of
Liposomal Drug Carriers to Ovarian Carcinoma. PLoS ONE 7(7): e41410.
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041410
Tumors
6. www.cdr.fi
Rodent Brain Imaging of Dopamine
Transporter Ligand [123I]β-CIT
5/23/2013 6Faculty of Pharmacy/ SPECT-CT/ CDR
unlesioned 6-OHDA
Striatal uptake 4h after i.v. administration
Modified from: “Advanced brain dopamine
transporter imaging in mice using small-
animal SPECT/CT”
Pitkonen M., Hippeläinen E., Raki M.,
Andressoo J, Urtti A, Männistö P, Savolainen
S., Saarma M., Bergström K. EJNMMI
Research 2012, 2:55
From S. Bäck et al., Division of Pharmacology and
Toxicology, University of Helsinki
7. www.cdr.fi
Dual Isotope Imaging of [123I]-NaI
Release from [99mTc]-labelled
Biomaterial
5/23/2013 7Faculty of Pharmacy/ SPECT-CT/ CDR
[99mTc]-labelled Biomaterial
[123I]-NaI
8. www.cdr.fi
Biodistribution of [99mTc]-labelled
Bone Marrow-Derived Mesenchymal
Stem Cells in Stroke Model
5/23/2013 8Faculty of Pharmacy/ SPECT-CT/ CDR
Rats subjected to middle cerebral artery
occulsion 24 hours before cell delivery
Majority of the stem cells are located in
the ischemic hemisphere 30 min after
intra-arterial infusion
Taken from: Raki, M. et al.,: Cell-based
therapies in stroke. Springer-Verlag. In
press.
9. www.cdr.fi
Preclinical to Clinical: in vivo brain
imaging from Mice to Men
5/23/2013 9Faculty of Pharmacy/ SPECT-CT/ CDR
24 h
1 h 4 h
Dopamine D2 DAT
Mouse
Human
Clinical images courtesy J. Kuikka
10. www.cdr.fi
CT Applications
5/23/2013 10Faculty of Pharmacy/ SPECT-CT/ CDR
CT images 8 week after surgical s.c.
implantation of biomaterial granules
Waselau M, et al. (2012) In vivo Effects of Bioactive Glass
S53P4 or Beta Tricalcium Phosphate on Osteogenic
Differentiation of Human Adipose Stem Cells after Incubation
with BMP-2. J Stem Cell Res Ther 2:125.
No contrast
agent
Vascular
contrast agent
Silvola J, et al. (2011) Uptake of 68gallium in
atherosclerotic plaques in LDLR-/-ApoB100/100 mice.
EJNMMI Research 1:14
11. www.cdr.fiFaculty of Pharmacy/ SPECT-CT/ CDR
Research collaboration:
IMI COMPACT consortium partners
5/23/2013 115/23/2013 11
• 7 EFPIA partners:
• 16 Academic and Biotech partners
• University of Helsinki/CDR
• Utrecht University Dept. of
Pharmaceutics
• University of Zurich
• University of Copenhagen
• Cardiff School of Pharmacy
and Pharmaceutical Sciences
• Stockholm University
• Norwegian Technical University
• University of Vienna
• Helmholtz Inst. for
Pharmaceutical Research
Saarland
• Utrecht University Veterinary
Immunology
• LMU Munich, Dept. of
Pharmacy
• University of Ghent
• PharmacoIdea Ltd.
• BioneerPharma
• Leiden University
• Oxford University
12. www.cdr.fi
• Theranostics imaging
and therapy - an action to
develop novel nanosized
systems for imaging-
guided drug delivery
• Chair of the Action:
• Professor Silvio Aime,
University of Turin, Italy
• Industries expressing an
interest in the COST
TD1004 Action:
• Imaging SpA
• Bayer Healthcare
Pharmaceuticals
• GE Healthcare
• Siemens Healthcare
• Guerbet
• Philips Diagnostics
Research Collaboration:
COST Action TD1004
5/23/2013 12Faculty of Pharmacy/ SPECT-CT/ CDR
13. www.cdr.fi
• Helsinki University Hospital (HUS Medical Imaging)
• BIU (Biomedicum Imaging Unit), University of Helsinki, Finland
• University of Helsinki, Finland
• Arto Urtti, Maart Saarma, Pekka Männistö, Raimo Tuominen, Marjo Yliperttula,
Anu Airaksinen
• University of Tarto, Estonia
• Pille Taba
• Regea Cell and Tissue Center, University of Tampere, Finland
• Susanne Miettinen, Martin Waselau
• University of Turku, Finland
• Anne Roivainen
• University of Eastern Finland
• Jukka Jolkkonen
• Finnish Red Cross Blood Service
• Saara Laitinen
5/23/2013 13
Research Collaboration:
Domestic and Regional Partners
Faculty of Pharmacy/ SPECT-CT/ CDR
14. www.cdr.fi
• SPECT/CT enables real-time morphological imaging and tracking of radiolabeled
substances in rodents at ultra-high resolution (< 0.5 mm), quantitatively, & in 3D.
• It is suitable for a variety of research applications in oncology, neurology,
cardiovascular pharmacology, target discovery, & gene expression studies; and,
also for pharmacodynamic, pharmacokinetic, and morphological measurements.
• We welcome industrial and academic collaborations in method development,
and we also provide fee-based contract imaging services. We offer:
Consultation, development, and execution of tailored experimental protocols or
specific portions of experimental protocols.
Radiochemical and radiopharmacy services for the synthesis of SPECT tracers
On-site facilities for housing and maintenance of the users’ animals. All aspects of
care & handling can be arranged.
Expertise of hospital physicist and access to MRI can be arranged for studies as
needed.
We take responsibility for use of radioactive materials, and we ensure appropriate
logistics for radioactive material and radioactive waste.
5/23/2013 14
Summary
Faculty of Pharmacy/ SPECT-CT/ CDR
Contact: Paul Bromann, PhD paul.bromann@helsinki.fi www.CDR.fi
