بسم الله الرحمن الرحيم Block: Head & Neck Structure and Function Biochemistry Lecture: Muscle contraction: Introduced by: Dr/Abousree El-Lethy
List the main biochemical components of muscle fiber & list its organelles in relation to their functions in muscle contraction.
List the main proteins composing the structure of sarcomere & describe the function of each.
List the main proteins composing thin & thick filaments & their relationships in skeletal muscles.
List the main accessory proteins that play an important role in muscle structure & function. Emphasize the function of dystrophin and related proteins.
Outline the biochemical events that occur during one cycle of muscle contraction & list the determinants that lead to relaxation.
List the different sources of energy for muscle contraction.
Describe the role of calcium ions in muscle contraction & relaxation
A- List the main contractile proteins of of muscle fiber The mass of a muscle is made up of 75% water and more than 20% protein.
B-List muscle fiber organelles (biochemical structures) in relation to their functions in muscle contraction. 1-Functional units of myofibrils is sarcomeres that has: 1) cytoplasm called sarcoplasm that contain
ATP, and phosphocreatine
Enzymes of glycolysis
2) Sarcoplasmic reticulum contains calcium to conduct nerve impulses. The muscle cell is called a muscle fiber with plasma membrane called sarcolemma Many nuclei and mitochondria (sarcosome)
2-A band: Dark band seen as part of striation (anisotropic band) 3-I band: light band area between the ends of the myosin (isotropic band) 4-Z-line: Membrane that separates sarcomeres 5-H zone: Area by which the two ends of the thin filaments fail to meet 6-Sarcomere is the functional unit composed of two myofilaments
Thick filaments (myosin)
Thin filaments (actin- tropinin-tropomyosin)
The main proteins composing the structure of sarcomere & functions 1-Thick filament that contain myosin protein (dark band) It contains myosin-binding protein C which binds at one end to the thick filament and the other to Actin. 2-Thin filaments are composed of: 1) Actin : It is globular protein formed of (G-actin & F-actin) Function: Actin forms cross bridges with myosin during muscle contraction. 2) Tropomyosin: It is a fibrous protein of two chains, alpha and beta, Function: it is attached to F-actin in the groove between its filaments to block myosin binding sites 3) Troponin complex: three polypeptides Troponin T which binds to tropomyosin Troponin I, inhibits the F-actin-myosin interaction binding during absence of Ca+2 of relaxed Striated Muscle. Troponin C: calmodulin for calcium
Main outline structure of sarcomere
Main accessory proteins that play an important role in muscle structure & function Titin Nebulin a-Actinin Desmin Dystrophin Calcineurin Myosin-.binding protein C Functions: Help in relaxation of muscle. Regulate assembly length of actin and filaments Stabilization of actin filaments. Maintaing structure of sarcomeres
Dystrophin is a cytoplasmic accessory protein, Function: 1-They connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. 2- Stabilizaton of membrane during contraction & relaxation
Main proteins composing thin & thick filaments & their relationships in skeletal muscles.
1-(Myosin= Thick Filaments)
It is an asymmetric hexamer consists of one pair of heavy chains and two pairs of light chains
Myosin has a fibrous tail consisting of two intertwined helices.
Each helix has a globular head portion
Globular head drives movement of actin filaments past myosin filaments.
Regulating ATPase functions depending on their phosphorylation status.
Thin Filaments =Actin It is monomeric or globular (G) actin, that upon addition of salts At physiological concentrations) G actine is polymerized to a highly viscous gel (filamentous or fibrous (F) actin Thin filaments contain the proteins actin, tropomyosin, and troponin Function: G-actinbinds globular myosin heads They inhibit the interaction between actin and myosin during relaxation
Sources of energy for muscle contraction (energy metabolism) .
Creatinephosphate Skeletal muscle contains phosphocreatine, which acts as an energy store for short-term (seconds) demands.
Skeletal muscle cannot contribute directly to blood glucose because it does not contain glucose-6-phosphatase.
Anaerobic metabolism in skeletal muscle produce Lactate (anaerobic glycolysis) then passes to liver to synthesize glucose, which can then return to muscle (the Cori cycle).
In the fed state, most glucose is used to synthesize glycogen
Free fatty acids & ketone bodies are a major source of energy Proteolysis supplies amino acids for gluconeogenesis.
Metabolism of branched-chain amino acids
Major amino acids emanating from muscle are alanine (destined mainly for gluconeogenesis in liver and forming part of the glucose-alanine cycle) and glutamine (destined mainly for the gut and kidneys).
Epinephrine stimulates glycogenolysis in skeletal muscle, whereas
Glucagon does not because of absence of its receptors. Insulin acts on skeletal muscle to increase uptake of glucose
Actin, myosin, tropomyosin, troponin complex (TpT, Tpl, and TpC), ATP, and Ca2+ are key constituents in relation to contraction.
The Ca2+ ATPase, the Ca2+ release channel, and calsequestrin are proteins involved in various aspects of Ca2+ metabolism in muscle.
Biochemical events that occur during one cycle of muscle contraction & list the determinants that lead to relaxation Muscle contraction subject to fine regulation via the nervous system (1)Discharge of motor neuron (2) Release of transmitter (acetylcholine) at motor endplate then binding receptors (3) Increased Na+ and K+ conductance in endplate membrane
Steps in Muscle Contraction (Resting sarcomere) Step 1: Head of myosin hydrolyzes ATP to ADP and Pi to result ADP-Pi- myosin complex (high-energy conformation.) Step 2: (in response to nerve/Ca2+ stimulation) Ca+ binds to troponin exposing active site on actin forming actinomycin complex Then binding to ADP-Pi- myosin complex Step 3: Promotes the release of Pi, which initiates the power stroke (conformational change in myosin heads ), then release of ADP Pulling of crossbridgeactin towards center of sarcomere(shortening )
Step 4: Myosin head binds another ATP Forming an actin-myosin-ATP complex. Step 5: (key component of relaxation) Myosin-ATP has a low affinity for actin, and actin is thus released. In step 4: If intracellular levels of ATP drop (eg, after death), ATP does not bind myosin head (step 4 above), and actin does not dissociate, and relaxation (step 5)does not occur.
Steps in relaxation Step 1: Ca2+ pumped back into sarcoplasmic reticulumbyCa2+ ATPase Step 2: Release of Ca2+ from troponinC Step 3:Troponin, via interaction with tropomyosin, inhibits further myosin head and F-actin interaction. Step 4: Presence of ATP, myosin head lead to release of F-actin. Step 5: The end of interaction between actin and myosin
The role of calcium ions in muscle contraction & relaxation During contraction Sarcolemma depolarization: Spreads to internal T tubule system Ca2+ is released from the SR and from the extracellular space Ca2+ interacts with calmodulin (TpC) and myosin light chain kinase to activate myosin (uncovering of myosin binding sites). Activated calmodulin activates the Myosin/ATPase (kinase) Activated kinase transfers phosphate from ATP to myosin cross bridges Phosphorylated cross bridges interact with actin to produce shortening (contraction)
During relaxation: SarcoplasmicCa + + reduced and pumped into the SR by ATP-driven Ca + + pump Troponin and tropomyosin, inhibits myosin head and F-actin interaction (covering of myosin binding sites) , and in the presence of ATP the myosin head detaches from the F-actin. Reference: Harper’s Illustrated Biochemistry Chapter 49:Muscle & the Cytoskeleton