Presentation by Sandip Banerjee, Kefelew B and Sintayehu Y at the 5th All Africa conference on animal production, Addis Ababa, Ethiopia, 25-28 October 2010.

1. Some hematological and biochemical parameters of
Arsi Bale sheep naturally infected with Facilola sp.
Sandip Banerjee, Kefelew B and Sintayehu Y
Department of Animal and Range Science
Hawassa University
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa:
Challenges and opportunities October 25-28, 2010.

2. Arsi Bale sheep is the most predominant
sheep in Hawassa
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa:
Challenges and opportunities October 25-28, 2010.

3. Liver fluke is one of the important causes of loss in carcass
weight
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa:
Challenges and opportunities October 25-28, 2010.

4. Damage to liver causes loss in the value of carcass,
besides death in many cases
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa: Challenges and opportunities
October 25-28, 2010.

5. Most of the times the whole organ is to be discarded
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa: Challenges and opportunities
October 25-28, 2010.

6. Different stages of infection
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa: Challenges and opportunities
October 25-28, 2010.

7. The serum biochemical traits were analyzed using
Humastar-300
Serum alkaline phosphatase (ALKP),
Serum aspartate aminotransferase (AST),
Serum aniline amino transferase, (ALT)
Serum cholesterol( CH),
Serum creatinine (CREA),
Serum total protein (TP)
and
Serum triglycerides (TRI)
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa: Challenges and opportunities
October 25-28, 2010.

8. Hematological traits were analyzed using
Abbott CELL-DYN 1800
White blood corpuscles (WBC),
Red blood corpuscles (RBC),
Lymphocytes (LYM),Total cell count (MID),
Granulocytes, (GRAN) ,Hemoglobin ( Hb),
Hematocrit (HCT),Mean corpuscular volume (MCV),
Mean corpuscular hemoglobin (MCH)
and
Mean corpuscular hemoglobin concentration (MCHC).
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa: Challenges and opportunities
October 25-28, 2010.

9. Parameters Infected Ram Non infected Ram Infected Ewe Non infected Ewe
Mean± SD
Range
Mean± SD
Range
Mean± SD
Range
Mean± SD
Range
ALKP‫٭٭‬
(IU/L)
423.0 ± 134.4ª
(254-622)
170.5± 17.6b
(158-183)
467.3±205.17 ª
(285-917)
163.5±22.35b
(136-202)
ALT‫٭‬
(IU/L)
23.6±5.4 ª
(16-32)
15.5± 4.94 b
(12-19)
25.0±15.8 ª
(10-61)
17.33±6.7 b
(10-25)
AST‫٭٭‬
(IU/L)
292.4±172.25 c
(107-535)
118.0± 4.24 ª
(115-121)
232.0±179.9 b c
(83-682)
165.83±37.8 ª b
(97-210)
CH‫٭٭‬
(mg/dl)
59.2±13.9bc
(38-79)
37.0± 11.3 ª
(29-45)
68.0±17.4c
(42-97)
44.16±8.32 ªb
(34-55)
CREA‫٭‬
(mg/dl)
.75±.36 ª
(.2-1.4)
.85±.07 ª
(.8-.9)
.84±.35 ª
(.2-1.3)
1.03±.1 b
(.9-1.2)
TP‫٭‬
(g/dl)
6.10±.79 ª
(5.1-7.4)
5.1± 1.13 b
(4.3-5.9)
5.8±1.11 ª
(4-7.5)
5.23±1.32 b
(3.6-7.2)
TRI‫٭‬
(mg/dl)
20.11±6.0 ª
(9-27)
15.5± 3.53 b
(13-18)
21.2±10.76 ª
(4-43)
15.16±4.5 b
(9-21)
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa:
Challenges and opportunities October 25-28, 2010.
Results for Serum Biochemical traits

10. Parameters I
Infected Ram Non infected Ram Infected Ewe Non infected Ewe
Mean± SD
Range
Mean± SD
Range
Mean± SD
Range
Mean± SD
Range
WBC‫٭‬
(k/µl) 30.10±13.2 ª
(16-46.8)
42.95± 12.51 b
(34.1-54.8)
39.8±16.7 b
(17-67.8)
44.21±16.09 b
(31.5-73)
LYM‫٭‬
(RM) 26.93±12.7 ª
(13.5-42.8)
38.5± 9.9 b
(31.5-41.5)
36.6±15.5 b
(16.4-62.7)
38.5±16.11 b
(25.5-65.6)
MID‫٭‬
(RM) 2.34±1.07 ª
(.6-4.0)
2.95±1.62 b
(1.8-4.1)
2.2±1.02 ª
(.8-3.5)
3.71±1.88 c
(1.8-6.3)
GRAN‫٭‬
(RM) .811±.61 ª
(.2-2.0)
1.4±1.13 b
(.6-2.2)
.94±.63 ª
(.1-1.7)
2.0±1.52 c
(.6-4.8)
RBC‫٭‬
(M/ µl) 4.78±1.01 ª
(3.43-6.31)
5.65±.35 b
(5.4-5.9)
5.34±1.27 b
(2.85-6.4)
5.49±.76 b
(4.05-6.24)
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa:
Challenges and opportunities October 25-28, 2010.
Results for some hematological traits-1

11. Parameters I
Infected Ram Non infected Ram Infected Ewe Non infected Ewe
Mean± SD
Range
Mean± SD
Range
Mean± SD
Range
Mean± SD
Range
HB‫٭‬
(g/dl)
9.87±2.63 ª
(4.4-11.9)
11.05± 1.34 ª
(10.1-12)
10.36±2.11 ª
(6-13.1)
12.36±3.8 b
(8.7-18.2)
HCT‫٭‬
(%) 16.32±2.8 ª
(13-20.8)
18.15±1.35 b
(17.2-19.1)
17.73±4.6 ªb
(8.7-22.2)
18.23±2.84 b
(12.7-20.5)
MCV‫٭‬
(fl)
34.64±2.62 ª
(31.8-40.6)
32.15± .353 b
(31.9-32.4)
32.64±1.006 b
(30.4-33.9)
32.51±.82 b
(31.4-33.5)
MCH‫٭‬
(pg)
15.78±3.3 ª
(12-21.3)
19.5± 1.13 b
(18.7-20.3)
19.32±3.7 b
(15.7-28.4)
18.15±2.3 b
(15.7-20.5)
MCHC‫٭‬
(g/dl)
50.11±10.4 ª
(31.9-67.1)
60.75±2.9 b
(58.7-62.8)
59.52±13.46 b
(47.7-93.1)
56.6±8.21 b
(47.3-64.5)
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa:
Challenges and opportunities October 25-28, 2010.
Results for some hematological traits-2

12. Results and Conclusion
Significant differences (P≤0.05)were observed in Serum Alkaline phosphatase (
ALKP) , serum aspartate aminotransferase (AST) and cholesterol( CH) levels of the
infected and non infected sheep.
There were differences in values of all the other parameters, both hematological
and serum biochemical parameters, though not significant.
It can thus be concluded that ALKP, AST and CH levels can indicate tehe extent
of hepatic damage due to Faciola sp. in sheep
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa:
Challenges and opportunities October 25-28, 2010.

13. Thank you
The 5th
All Africa Conference on Animal Agriculture
Commercialization of Livestock Agriculture in Africa:
Challenges and opportunities October 25-28, 2010.