Presentation from the Nov 2008 Alcoa Forum. Thanks for the upload. Note - the majority of the site work is related to Do Blackert - i updated the pdf to include Dons link information. The rest of the information is from the presentation.

1. REMEDIATION USING SOY BASED AEROBICCO-METABOLISM FOR REMOVAL OF CHLORINATED HYDROCARBONS FROM GROUNDWATERForum Presentation Neale Misquitta and Dale Foster Key Environmental, Inc. 412-279-3363

2. Update Slide 2008 Presentation 2009 and Others www.soygold.com/products/.../sg5000_RemediationTechnologyUpdate.pdf www.eswp.com/brownfields/program09.htm cluin.org/search/default.cfm?search_term=aerobic&t...41 www.battelle.org/ASSETS/.../Presentations.pdf

4. DEVELOPMENT AND TESTING

6. BIODEGRADABLE FOOD GRADE SURFACTANT ADDED TO SOY METHYL ESTER Forms emulsion with water Significantly enhances dispersion in aquifer Provides co-solvent effect to strip hydrocarbons from water and soil, minimizing rebound

7. DEVELOPMENT, TESTING & APPLICATION Chlorinated hydrocarbon concentrations were significantly reduced at a site where toluene was co-mingled in groundwater (toluene oxygenase enzyme process) Needed dispersible, biodegradable, food grade material to replace toluene Jar and column tests indicated that the soy methyl ester surfactant blend had best dispersion characteristics of any carbon sources tested First field pilot injections in 2002. Six pilot tests and six full scale applications conducted between 2002 & 2007 – Most of the work by Don Blackert

9. Two monitoring wells

10. 5 years of AS/SVEprior to injection

11. Results From First Field Pilot TestTotal Halogenated CompoundsSource Area Well - 2002 99.9% Reduction 5 years 1 year

13. 7 injection wells

14. 5 monitoring wells

15. COCs

16. Carbon tetrachloride

17. Chloroform

18. Tetrachloroethene

19. Fluorocarbons

22. 6 injection wells

23. 10 monitoring points

24. COCs

25. Tetrachloroethene

26. Carbon Tetrachloride

27. Chloroform

28. Fluorocarbons

30. Average Chloride ConcentrationsFirst Pilot Test

31. Heterotrophic Plate Count

32. Dissolved Oxygen Concentration

34. Site-wide application December 2004

35. Design in progress for remediation of off-site plume during 2008

38. TCE

40. Source area remediation approximately 1 acre

41. Site wide application reduced concentrations approximately 84% in the first year

42. Site-wide reductions through November 2007

43. TCE 98%

45. Successful removal of chlorinated hydrocarbons

46. Recalcitrant CompoundsCo-metabolic Pilot TestSuperfund Site – West Virginia

47. Chloroethane, Dimethylthiourea, Benzene DegradationCo-metabolic Pilot TestSuperfund Site – West Virginia

49. Increased from 64,000 cfu/ml to 1.2 million cfu/ml during the first 3 months

50. Plume Core Area Maximum single well Population Increase

51. From non-detectable concentrations to 5.5 million cfu/ml

52. Plume Fringe Area Average Microbial Populations

53. Increased from 45,000 cfu/ml to over 10 million cfu/ml during the first 3 months

54. Plume Fringe Area Maximum single well Population Increase

59. DCA 92%

60. TCE 90%

61. PCE 98%

64. Effective for a wide variety of chlorinated hydrocarbons without generation of daughter products

65. Rapid and cost effective

66. 95-99% removal efficiency typical in 6-12 months

67. Combined microbial and co-solvent processes minimize post treatment rebound by treating both groundwater and saturated soil

68. Particularly well suited for:

69. Sites where AS/VE systems already exist and chlorinated hydrocarbons persist

70. Small, high concentration “hot spots”

71. Plume cut-off systems