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Remedition Using Soy Based Aerobic Co-Metabolism for Removal of Chlorinated Hydrocarbons from Groundwater
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Remedition Using Soy Based Aerobic Co-Metabolism for Removal of Chlorinated Hydrocarbons from Groundwater

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Presentation from the Nov 2008 Alcoa Forum. Thanks for the upload. Note - the majority of the site work is related to Do Blackert - i updated the pdf to include Dons link information. The rest of …

Presentation from the Nov 2008 Alcoa Forum. Thanks for the upload. Note - the majority of the site work is related to Do Blackert - i updated the pdf to include Dons link information. The rest of the information is from the presentation.

Published in: Technology, Business

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  • 1. REMEDIATION USING SOY BASED AEROBICCO-METABOLISM FOR REMOVAL OF CHLORINATED HYDROCARBONS FROM GROUNDWATERForum Presentation
    Neale Misquitta and
    Dale Foster
    Key Environmental, Inc.
    412-279-3363
  • 2. Update Slide
    2008 Presentation
    2009 and Others
    www.soygold.com/products/.../sg5000_RemediationTechnologyUpdate.pdf
    www.eswp.com/brownfields/program09.htm
    cluin.org/search/default.cfm?search_term=aerobic&t...41
    www.battelle.org/ASSETS/.../Presentations.pdf
  • 3. PRESENTATION
    • PROCESS BASICS
    • 4. DEVELOPMENT AND TESTING
    • 5. HIGHLIGHTS FROM 6 PILOT TESTS AND 6 FULL SCALE TESTS
  • BASICS OF SOY BASED AEROBIC C0-METABOLISM
    Inject and disperse highly biodegradable soy methyl ester
    Create and maintain aerobic conditions
    Indigenous aerobic microbe populations increase by up to several orders of magnitude
    In the process of metabolizing the methyl ester, microbes produce oxygenase enzymes
    Enzymes capable of breaking molecular bonds of chlorinated molecules
    Chlorinated hydrocarbons degraded to basic components
  • 6. BIODEGRADABLE FOOD GRADE SURFACTANT ADDED TO SOY METHYL ESTER
    Forms emulsion with water
    Significantly enhances dispersion in aquifer
    Provides co-solvent effect to strip hydrocarbons from water and soil, minimizing rebound
  • 7. DEVELOPMENT, TESTING & APPLICATION
    Chlorinated hydrocarbon concentrations were significantly reduced at a site where toluene was co-mingled in groundwater (toluene oxygenase enzyme process)
    Needed dispersible, biodegradable, food
    grade material to replace toluene
    Jar and column tests indicated that the soy methyl ester surfactant blend had best dispersion characteristics of any carbon sources tested
    First field pilot injections in 2002. Six pilot tests and six full scale applications conducted between 2002 & 2007 – Most of the work by Don Blackert
  • 8. FIRST FIELD SCALE PILOT – 2002
    • One injection well
    • 9. Two monitoring wells
    • 10. 5 years of AS/SVE
    prior to injection
  • 11. Results From First Field Pilot TestTotal Halogenated CompoundsSource Area Well - 2002
    99.9% Reduction
    5 years
    1 year
  • 12. First Sitewide Application - 2002
    • Site size – ½ acre
    • 13. 7 injection wells
    • 14. 5 monitoring wells
    • 15. COCs
    • 16. Carbon tetrachloride
    • 17. Chloroform
    • 18. Tetrachloroethene
    • 19. Fluorocarbons
    • 20. >99% reductions in less than one year. Minimal rebound 2 years after shutdown
  • Results From First Full Scale RemediationSitewide Average ConcentrationTotal Halogenated Compounds
    • 99% reduction
    first year
    • 93% reduction after
    2½ years of rebound
    5 years 1 year 2½ years
  • 21. Second Full Scale Remediation 2003Sitewide Average ConcentrationTotal Halogenated Compounds
    1 year
    • 94% reduction 28 months
    after shutdown
    2½ years
    1 year
    7 years
  • 30. Average Chloride ConcentrationsFirst Pilot Test
  • 31. Heterotrophic Plate Count
  • 32. Dissolved Oxygen Concentration
  • 33. KANSAS CITY, KANSAS
    • Pilot test September 2003
    • 34. Site-wide application December 2004
    • 35. Design in progress for remediation of
    off-site plume during 2008
  • 36. KANSAS CITY PILOT TEST 2003
    • 2 Injection wells
    • 37. 4 Monitoring
    points
    • 99% reduction
    in 9 months
  • KANSAS CITY, KANSASFULL SCALE REMEDIATION SUMMARY
    • PPM Concentrations of TCE and DCE
    • 40. Source area remediation approximately 1 acre
    • 41. Site wide application reduced concentrations approximately 84% in the first year
    • 42. Site-wide reductions through November 2007
    • 43. TCE 98%
    • 44. CIS 1,2 DCE 97%
  • TESTS 5 AND 6 SUPERFUND SITE - WEST VIRGINIA
    • Two Test areas 2004 – Plume core and
    plume fringe
    • Each test area included 2 injection
    points and 4 monitoring points
    • First chemical limitations observed
    • 45. Successful removal of chlorinated
    hydrocarbons
  • 46. Recalcitrant CompoundsCo-metabolic Pilot TestSuperfund Site – West Virginia
  • 47. Chloroethane, Dimethylthiourea, Benzene DegradationCo-metabolic Pilot TestSuperfund Site – West Virginia
  • 48. Microbial ResponseSuperfund Site – West Virginia
    • Plume Core Area Average Microbial Populations
    • 49. Increased from 64,000 cfu/ml to 1.2 million cfu/ml during the first 3 months
    • 50. Plume Core Area Maximum single well Population Increase
    • 51. From non-detectable concentrations to 5.5 million cfu/ml
    • 52. Plume Fringe Area Average Microbial Populations
    • 53. Increased from 45,000 cfu/ml to over 10 million cfu/ml during the first 3 months
    • 54. Plume Fringe Area Maximum single well Population Increase
    • 55. From 51,000 cfu/ml to 48 million cfu/ml
  • Field Pilot Test – Ohio 2005
    • Two injection points – 5 monitoring points
    • 56. First (and only) test determined to be unsuccessful for all chlorinated hydrocarbons present in groundwater
    (TCE, PCE, DCE, DCA)
    • pH >11 precluded adequate microbial activity
    • 57. Provided additional verification of the importance of biological processes
  • Bench Scale Verification of Microbial Response to pH
  • 58. Milwaukee, Wisconsin2005 and 2007 Summary
    Large scale test initiated in 2005 (16 wells)
    Remediation area expanded up-gradient to source area beneath active manufacturing building during 2007
    Sitewide reduction March through October 2007
  • Milwaukee, Wisconsin2005 and 2007 Summary (continued)
    Saturated zone soil analysis indicated greater than 90% reduction in several chlorinated hydrocarbons in 5 months
    Operation terminated in 2008
    “Case Closure” pending approval
    from WDNR
  • 63. Summary & ConclusionsAerobic Co-metabolism
    • Safe – In Situ process
    • 64. Effective for a wide variety of chlorinated hydrocarbons without generation of daughter products
    • 65. Rapid and cost effective
    • 66. 95-99% removal efficiency typical in 6-12 months
    • 67. Combined microbial and co-solvent processes minimize post treatment rebound by treating both groundwater and saturated soil
    • 68. Particularly well suited for:
    • 69. Sites where AS/VE systems already exist and chlorinated hydrocarbons persist
    • 70. Small, high concentration “hot spots”
    • 71. Plume cut-off systems