1. 阿魏酸對人類肺癌細胞之抗腫瘤作用
Anti-tumor effect of ferulic acid on human lung cancer H1299 cells
長榮大學生物科技學系
陳宥安
實習單位:高雄醫學大學生物科技系
指導老師: 邱建智老師
Abstract
由過去研究發現，阿魏酸Ferulic acid (FA) 在藥理活性上具抗發炎、抑制血栓形成、抗自由基、抑制腫瘤細
胞生長及調節人體免疫功能的作用等等的活性。本論文主要以人類非小細胞肺癌細胞株作為研究對象，探討
FA引起H1299細胞生長及轉移的抑制能力，並藉由蛋白質表現的分析，探討其對肺癌細胞的作用機制。研究
結果顯示，FA轉移能力及抑制活性，與FA處理濃度和時間有關，在高濃度FA有明顯的生長抑制活性、轉移
能力也下降。利用PI的染色結果，FA能夠引起H1299細胞細胞週期停滯於G2/M期及誘導H1299細胞內ROS表
現下降，及不會導致細胞內粒線體膜電位下降。以明膠蛋白酵素電泳法 zymography assay進行肺癌細胞外培
養基的蛋白質分析，顯示FA處理後的 MMP-9及MMP-2 活性有明顯降低的趨勢。此外，以Migration assay及
Invasion assay進行FA對肺癌細胞作用的評估結果，也證實肺癌細胞在高濃度的轉移能力可被FA所抑制。綜合
上述結果，我們發現了FA萃取物與細胞轉移、移動能力有關，表示了天然植物所分離之物質FA在化學預防
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(chemoprevention)中有希望能成為癌症預防的新策略。
Experimental results FA
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vehicle 0.03 0.06 0.15 0.3 0.6 (mM)
30
25
Denuded zone
Denude zone (%)
20
0h
15
10
14 h 5
0
con 0.03 0,06 0,15 0.3 0.6
Fig 4. NSCLC H1299 cells were incubated with a series of FA dose (mM)
different concentration of FA for 14hr to observation cells
Fig 1. Structure of Ferulic acid migration.
Vehicle 0.03 0.06 120
cell number
140 100
24 hr
invasion index (%)
48 hr
120 80
viable cell number (%)
100 60
80 40
60 20
40 0
0.15 0.3 0.6 (mM)
con 0.03 0.06 0.15 0.3 0.6
20 FA Dose (mM)
0 Fig 5. NSCLC H1299 cells were incubated with a series of different concentration
Control 0.03 0.06 0.15 0.3 0.6
FA Dose (mM)
of FA for 16hr to observation cells invasion.
Fig 2. NSCLC H1299 cells were incubated with a (mM)
series of different concentration of FA for 24 and Con 0.03 0.06 0.15 0.3 0.6
48 hr to observation cell viability.
_________________ FA
0 0.03 0.06 0.15 0.3 0.6 (mM)
MMP-2
MMP-9
120 120
100 100 Fig 6. NSCLC H1299 cells were incubated with a series of different concentration
Fold relative activity (%)
Flod relative activity (%)
80 80
of FA for 16hr to observation cell cycle distribution through flow cytometric
60 60
analysis.
40 40
20
0
20
0
Discussion
con 0.03 0.06 0.15 0.3 0.6 con 0.03 0.06 0.15 0.3 0.6
FA Dose (mM) FA Dose (mM)
1、The inhibitory effect of FA on migration of lung cancer cells,
Fig 3. NSCLC H1299 cells were incubated with a suggesting that FA could be a chemoprevention agent in the
series of different concentration of FA for 24hr after future。
take supernatant to observation matrix metallopro-
teinases (MMP-2 and MMP-9) activity.