Imagine that you want to analyze a sample of aspirin by TLC, with a mobile phase of 10:90 ethyl acetate:hexanes. In order to prepare your sample to be spotted on the TLC plate you decide to dissolve your sample in 100% ethyl acetate (assume that it is completely soluble). In two sentences or less explain if this is an acceptable way to prepare your sample in order to be “spotted†on the TLC plate (assume the concentration is correct).
please explain correctly
Solution
When spotting on TLC you should dissolve your compound in a suitable solvent. If you have a solid sample and you place on TLC it will not get spotted on TLC. If you have a liquid sample and you spot on TLC it will be too concentrated spot and while developing the TLC, you will observe a borad peak, which will be difficult to analyase. Hence, you should have to have a minimum dilute solution of your compound which need to be spotted on TLC. After spotting on TLC and after few minutes, the compound will get absorbed on TLC and the solvent will evaporate.
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Imagine that you want to analyze a sample of aspirin by TLC- with a mo (1).docx
1. Imagine that you want to analyze a sample of aspirin by TLC, with a mobile phase of 10:90 ethyl
acetate:hexanes. In order to prepare your sample to be spotted on the TLC plate you decide to
dissolve your sample in 100% ethyl acetate (assume that it is completely soluble). In two
sentences or less explain if this is an acceptable way to prepare your sample in order to be
“spotted― on the TLC plate (assume the concentration is correct).
please explain correctly
Solution
When spotting on TLC you should dissolve your compound in a suitable solvent. If you have a
solid sample and you place on TLC it will not get spotted on TLC. If you have a liquid sample
and you spot on TLC it will be too concentrated spot and while developing the TLC, you will
observe a borad peak, which will be difficult to analyase. Hence, you should have to have a
minimum dilute solution of your compound which need to be spotted on TLC. After spotting on
TLC and after few minutes, the compound will get absorbed on TLC and the solvent will
evaporate.
