2. INTRODUCTION
Chromatography –’colour’
and ‘to write”.
Basically it is a broad range of
physical methods used to
separate and /or to analyse
complex mixtures.
Components to be separated
are distributed between two
phases :a stationary phase
bed and a mobile phase
which flows through the
stationary bed.
Schematic Diagram of GC
3. Individual species are retained by the stationary
phase (packing) based on various interactions
such as surface adsorption, relative solubility of
the sample in the mobile phase and charge.
4. APPLICATIONS
Exhaled species often reflect the health
conditions of the human body.
To date, many investigations have been
conducted for diagnostics of health
conditions and disease based on analysis of
breath air . Breath analysis has many
applications and so is of great interest.
5. Exhaled NO: It’s the most interesting target of
medical gas analysis is exhaled nitric oxide
(eNO), especially as a marker of asthma.
Halitosis: Halitosis is caused by NH3 , amines,
and most importantly certain sulfur gases, such
as H2S, CH3SH, dimethylsulfide (DMS).