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CHROMATOGRAPHY




   Biochemistry of Medics   1
Chromatography




       Biochemistry of Medics   2
Chromatography




       Biochemistry of Medics   3
Chromatography- Principle




        Biochemistry of Medics   4
Chromatography- Classification




       Biochemistry of Medics    5
Chromatography- Classification(Contd.)




         Biochemistry of Medics          6
Partition Chromatography




        Biochemistry of Medics   7
Paper chromatography




       Biochemistry of Medics   8
Paper chromatography




       Biochemistry of Medics   9
Paper chromatography- Procedure




       Biochemistry of Medics     10
Paper chromatography- Procedure




        Biochemistry of Medics    11
Ascending and Descending Paper Chromatography




           Biochemistry of Medics               12
Paper Chromatography -Analysis




        Biochemistry of Medics   13
Rƒ value




           Biochemistry of Medics   14
Two dimensional chromatography




       Biochemistry of Medics    15
Significance of Paper chromatography




        Biochemistry of Medics         16
Thin layer chromatography (TLC)




        Biochemistry of Medics    17
TLC- Procedure




        Biochemistry of Medics   18
TLC -Overview




           Biochemistry of Medics   19
Significance of TLC




        Biochemistry of Medics   20
Advantages of TLC over paper chromatography




           Biochemistry of Medics             21
2)Adsorption chromatography




       Biochemistry of Medics   22
2)Adsorption chromatography- Elution




         Biochemistry of Medics        23
Adsorption chromatography




The substance loosely adsorbed to the stationary medium comes in the earlier fraction.

                       Biochemistry of Medics                                        24
Ion – Exchange chromatography




        Biochemistry of Medics   25
Ion – Exchange chromatography




       Biochemistry of Medics   26
Ion – Exchange chromatography- Procedure




          Biochemistry of Medics           27
Ion – Exchange chromatography-
Procedure (Contd.)




         Biochemistry of Medics   28
Types of ion exchanges resins




        Biochemistry of Medics   29
Ion – Exchange chromatography-
            An Overview




          Biochemistry of Medics   30
4) Gel filtration chromatography or
Molecular Sieve chromatography




        Biochemistry of Medics        31
Gel filtration chromatography or
Molecular Sieve chromatography




        Biochemistry of Medics     32
Gel filtration chromatography-An
Overview




 Larger particles come out first, while smaller particles come in later fractions
                   Biochemistry of Medics                                           33
5) Affinity chromatography




        Biochemistry of Medics   34
Affinity chromatography- Significance




         Biochemistry of Medics         35
Affinity chromatography- Significance
By using antibodies, antigens can be easily separated.
Conversely, antibodies can be purified by passing through a column
containing the antigen.




 Specific Antibody binds to a specific antigen attached to the stationary medium, the
remaining antibodies come out in earlier fractions.          Biochemistry of Medics
                                                                                        36
6) High performance liquid chromatography-




          Biochemistry of Medics             37
High performance liquid chromatography-




          Biochemistry of Medics          38
High performance liquid chromatography-
              Significance




          Biochemistry of Medics          39
High performance liquid
chromatography- Apparatus




       Biochemistry of Medics   40
6) Gas liquid chromatography-




        Biochemistry of Medics   41
Gas liquid chromatography-




        Biochemistry of Medics   42
Gas liquid chromatography-Apparatus




        Biochemistry of Medics        43
Summary




      Biochemistry of Medics   44

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