Biology PBL

1. Mutation Illustration

2. Our Organism
• Chromosome 3 has 1561 nucleotides, and is the
chromosome where the coding for a tail/ tail
type is found. In our organism, this expresses
the two-fin tail allele.

3. Chromosome 3
•
1 gtgtccccgg agctgaaaga tcgcaaagag gatgcgaaag ggatggagga cgaaggccag 61 accaaaatca
agcagaggcg aagtcggacc aatttcaccc tggaacaact caatgagctg 121 gagaggcttt ttgacgagac ccactatccc
gacgccttca tgcgagagga actgagccag 181 cgactgggcc tgtcggaggc ccgagtgcag gtttggtttc aaaatcgaag
agctaaatgt 241 agaaaacaag aaaatcaact ccataaaggt gttctcatag gggccgccag ccagtttgaa 301
gcttgtagag tcgcacctta tgtcaacgta ggtgctttaa ggatgccatt tcagcaggtt 361 caggcgcagc tgcagctgga
cagcgctgtg gcgcacgcgc accaccacct gcatccgcac 421 ctggccgcgc acgcgcccta catgatgttc ccagcaccgc
ccttcggact gccgctcgcc 481 acgctggccg cggattcggc ttccgccgcc tcggtagtgg cggccgcagc agccgccaag
541 accaccagca aggactccag catcgccgat ctcagactga aagccaaaaa gcacgccgca 601 gccctgggtc
tgtgacgcca acgccagcac caatgtcgcg cctgtcccgc ggcactcagc 661 ctgcacgccc tccgcgcccc gctgcttctc
cgttacccct ttgagacctc gggagccggc 721 cctcttcccg cctcactgac catccctcgt cccctatcgc atcttggact
cggaaagcca 781 gactccacgc aggaccaggg atctcacgag gcacgcaggc tccgtggctc ctgcccgttt 841
tcctactcga gggcctagaa ttgggttttg taggagcggg tttgggggag tctggagaga 901 gactggacag ggtagtgctg
gaaccgcgga gtttggctca ccgcaaagct acaacgatgg 961 actcttgcat agaaaaaaaa aatcttgtta acaatgaaaa
aatgagcaaa caaaaaaatc 1021 gaaagacaaa cgggagagaa aaagaggaag gcaacttatt tcttaactgc
tatttggcag 1081 aagctgaaat tggagaacca aggagcaaaa acaaatttta aaattaaagt attttataca 1141
tttaaaaata tggaaaaaca acccagacga ttctcgagag actgggggga gttaccaact 1201 taaatgtgtg
ttttaaaaaa tgcgctaaga aggcaaagca gaaagaagag gtatacttat 1261 ttaaaaaact aagatgaaaa
aagtgcgcag gtgggaagtt cacaggtttt gaaactgacc 1321 tttttctgcg aagttcacgt taatacgaga aatttgatga
gagaggcggg cctcctttta 1381 cgttgaatca gatgctttga gtttaaaccc accatgtatg gaagagcaag
aaaagagaaa 1441 atattaaaac gaggagagag aaaaataatg gcaaaactgt ctggactgct gacagtaaat 1501
tccggtttgc atggaaaaaa aaaaaaaaaa aaaaaaaaaa a

4. Chromosome 3: Two-Fin Tail

5. Oh My! A Mutation Occurred!!
• This is the original 290 nucleotides in the gene :
241 agaaaacaag aaaatcaact ccataaaggt gttctcatag gggccgccag ccagtttgaa 301 gcttgtagag tcgcacctta
tgtcaacgta ggtgctttaa ggatgccatt tcagcaggtt 361 caggcgcagc tgcagctgga cagcgctgtg gcgcacgcgc accaccacct
gcatccgcac 421 ctggccgcgc acgcgcccta catgatgttc ccagcaccgc ccttcggact gccgctcgcc 481 acgctggccg
cggattcggc ttccgccgcc tcggtagtgg cggccgcagc agccgccaag
• However in the sequence below, the 290 nucleotides have been
deleted:
1 gtgtccccgg agctgaaaga tcgcaaagag gatgcgaaag ggatggagga cgaaggccag 61 accaaaatca agcagaggcg
aagtcggacc aatttcaccc tggaacaact caatgagctg 121 gagaggcttt ttgacgagac ccactatccc gacgccttca tgcgagagga
actgagccag 181 cgactgggcc tgtcggaggc ccgagtgcag gtttggtttc aaaatcgaag agctaaatgt 541 accaccagca
aggactccag catcgccgat ctcagactga aagccaaaaa gcacgccgca 601 gccctgggtc tgtgacgcca acgccagcac caatgtcgcg
cctgtcccgc ggcactcagc 661 ctgcacgccc tccgcgcccc gctgcttctc cgttacccct ttgagacctc gggagccggc 721 cctcttcccg
cctcactgac catccctcgt cccctatcgc atcttggact cggaaagcca 781 gactccacgc aggaccaggg atctcacgag gcacgcaggc
tccgtggctc ctgcccgttt 841 tcctactcga gggcctagaa ttgggttttg taggagcggg tttgggggag tctggagaga 901 gactggacag
ggtagtgctg gaaccgcgga gtttggctca ccgcaaagct acaacgatgg 961 actcttgcat agaaaaaaaa aatcttgtta acaatgaaaa
aatgagcaaa caaaaaaatc 1021 gaaagacaaa cgggagagaa aaagaggaag gcaacttatt tcttaactgc tatttggcag 1081
aagctgaaat tggagaacca aggagcaaaa acaaatttta aaattaaagt attttataca 1141 tttaaaaata tggaaaaaca acccagacga
ttctcgagag actgggggga gttaccaact 1201 taaatgtgtg ttttaaaaaa tgcgctaaga aggcaaagca gaaagaagag gtatacttat
1261 ttaaaaaact aagatgaaaa aagtgcgcag gtgggaagtt cacaggtttt gaaactgacc 1321 tttttctgcg aagttcacgt
taatacgaga aatttgatga gagaggcggg cctcctttta 1381 cgttgaatca gatgctttga gtttaaaccc accatgtatg gaagagcaag
aaaagagaaa 1441 atattaaaac gaggagagag aaaaataatg gcaaaactgt ctggactgct gacagtaaat 1501 tccggtttgc
atggaaaaaa aaaaaaaaaa aaaaaaaaaa a

6. The Result
• The result of this mutation causes the tail to not split
into two fins, but stay as a whole.