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Explain the heterogenous subunits of Rpb1 by answering these questions. What is the difference
between IIo, IIa and IIb? Which enzymes convert IIa to IIo, IIo to IIa and IIa to IIb?
Solution
Rpb I is a subunit of DNA directed RNA polymerase II which is encoded by POLR2A GENE.
IIa - stabilises TATA binding proteins (TBP ) and TAF binding ( TBP associated factors ) . This
has one subunit.
IIb - this GTF HAS 2 subunits. intermediate in the recruitment of RNA polymerase II. Influences
selection of the start point of transcription. This protein would enter the pre initiation complex
after TBP. This protein bridges the TATA bound TBP and polymerase.
*There appears to be a mistype in IIo as none of the transcription factors are named as IIo, but
yes there is a transcription factor as IId. Kindly leave in the comments if that is the same you
want details about and feel free to ask any question.

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