Thinking about antibody validation

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St John's Laboratory Ltd

Addressing the growing concerns associated with the use of commercial antibodies in research and in diagnostics.

Science

There is no governing agency
monitoring what can be
sold in the antibody based
assay market.

• High standards for antibody validation
• Greater confidence
• Eliminate wasted time and money
• Incentive to validate

EVERYONE
Suppliers
Distributors
Laboratory directors
Scientists

1. Bordeaux J, Welsh A, Agarwal S, Killiam E, BaqueroM, Hanna J, Anagnostou V, Rimm D.
Antibody validation. Biotechniques. 2010 Mar;48(3):197-209.
2. Welsh AW, Lannin DR, Young GS, Sherman ME, Figueroa JD, Henry NL, Ryden L, Kim C, Love
RR, Schiff R, Rimm DL. Cytoplasmic estrogen receptor in breast cancer. Clin Cancer Res. 2012
Jan 1;18(1):118-26.
3. Lukinavičius G, Lavogina D, Gönczy P, Johnsson K. Commercial Cdk1 antibodies recognize the
centrosomal protein Cep152. Biotechniques. 2013 Sep;55(3):111-4.
4. Couchman JR: Commercial antibodies: the good, bad, and really ugly. J Histochem
Cytochem. 2009; 57(1): 7–8. PubMed Abstract | Publisher Full Text | Free Full Text
5. Saper CB: An open letter to our readers on the use of antibodies. J Comp Neurol. 2005; 493(4):
477–8.PubMed Abstract | Publisher Full Text
6. Voskuil J: The troubles with commercial research antibodies dissected. Everest Biotech
Blog. 2013

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