1. @StJohnsLabs
www.stjohnslabs.com

3. A Standard for
Antibody Validation !

5. There is no governing
agency monitoring what can
be sold in the anti-body
based assay market.

7. Suppliers
Distributors
Laboratory directors
Scientists

8. • Poor standards for antibody validation
• Unreliable results
•Wasted time and money
• Reproducibility problems

10. Find out in Part Two.

11. 1. Bordeaux J, Welsh A, Agarwal S, Killiam E, BaqueroM, Hanna J, Anagnostou V, Rimm D.
Antibody validation. Biotechniques. 2010 Mar;48(3):197-209.
2. Welsh AW, Lannin DR, Young GS, Sherman ME, Figueroa JD, Henry NL, Ryden L, Kim C, Love
RR, Schiff R, Rimm DL. Cytoplasmic estrogen receptor in breast cancer. Clin Cancer Res. 2012
Jan 1;18(1):118-26.
3. Lukinavičius G, Lavogina D, Gönczy P, Johnsson K. Commercial Cdk1 antibodies recognize the
centrosomal protein Cep152. Biotechniques. 2013 Sep;55(3):111-4.
4. Couchman JR: Commercial antibodies: the good, bad, and really ugly. J Histochem
Cytochem. 2009; 57(1): 7–8. PubMed Abstract | Publisher Full Text | Free Full Text
5. Saper CB: An open letter to our readers on the use of antibodies. J Comp Neurol. 2005; 493(4):
477–8.PubMed Abstract | Publisher Full Text
6. Voskuil J: The troubles with commercial research antibodies dissected. Everest Biotech
Blog. 2013