Participants of the workshop learn the necessary background information and techniques to diagnose Sars-CoV-2 using the mobile diagnostic laboratory. The laboratory is shipped ready to use with all devices, reagents, certificates, and protocols. After one day of preparation together with a local assistant, a five-day course is given where every step is carried out by each participant. Experts accompany the learning process with written teaching materials, video training, virtual live coaching, and short exams to verify the learned content.
5. Bio.Kitchen is an open community lab for tech-talents
5 workstations: micro- and molecular biology, chemistry, lab-on-Chip and lab automation
Rüdiger
Trojok
Lab Lead
Leo
Sprengel
Lab Assistant
Andreas
Jefimowicz
Lab Assistant
6.
7. Tools & Tech: rapid prototyping for agile life science
DNA Diagnostics Bentolab
Open Source Lab Hardware Open Source Pipetting
Robot
DNA Sequencer MinION
DNA Printer Kilobaser
Rapid Prototyping (digital)
Microfluidics
Cell Free Protein
Synthesis
Open Reagents
Collection
15. PCR testing for COVID-19 needs to happen while the infection
is ongoing
https://www.rki.de/DE/Content/InfAZ/N/Neuartiges_Coronavirus/Steckbrief.html#doc13776792bodyText8
https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(20)30566-
3/fulltext?fbclid=IwAR3Hyjh3ax53XAGCxWpC3aNWx1k8_VEPxiOlfptMkVUinaN_MWHodgsIy1k
16. COVID-19 testing needs to happen rapidly to keep infectious
zones local and under control.
21. RT-QPCR is one globally accepted “Gold Standard” for testing on COVID-19. It is
expensive and time consuming. In addition handling in the lab is complex.
QPCR Thermocycler
22. RT-LAMP provides a cheaper and more reliable protocol compared to RT-QPCR.
Devices are cheaper , need less expertise. Time to result can be as low as 30min
Heat block
reaction dependent dye for
visual readout
23. qPCR
LAMP
Sample
Sample preparation
(RNA extraction)
Sample preparation
(RNA extraction)
RNA amplification
RNA amplification
Analysis & report
RT-qPCR protocol
with results in 3,5
hours
Combination of qPCR and LAMP
24
0 0,5 1,0 1,5 2,0 2,5
Time
[hours]
3,0 3,5
Sample
Analysis & report
RUO LAMP quick test with
results in 2 hours without
thermocyclers
Test strategy:
Perform LAMP with all patient
samples.
Re-test LAMP-positive samples
with qPCR.
RT-LAMP = Reverse
Transcription Loop- Mediated
Isothermal Amplification.
24. RT-QPCR has been automated by various biotech companies as desktop solutions...
27. 28
Mobile Sars-COV-2
Detection Lab
Mobile Lab
Core Technology
Training
Support
qPCR & LAMP technology
and workflow for
Diagnostic
Easy to transport and
ready to use in 24 h
education courses to
train users
Supply of Consumables,
Reagents & online help
desk
28.
29. S2 Sample preparation room
S2 Patient
sampling
room
S1 Amplification
room
Patient
registration
& entrance
Patient
exit
S1 Reagent
preparation room
Patient
sample
Virus RNA
PCR & LAMP
samples
Patient sample RNA Extraction
RT-LAMP & RT-QPCR
Mastermix Preparation
RT-LAMP & RT-
QPCR
Amplification
Patient
sampling
Covid-19 Diagnostic process using open source
technology and reagents with the Bio.Kitchen Mobile Lab
31. UnternehmerTUM GmbH / 21.11.19
Research use only LAMP quick test give results in 1.5h
without the need for thermocyclers or centrifuges
https://international.neb.com/protocols/2016/08/15/warmstart-colorimetric-lamp-2x-master-mix-typical-lamp-protocol-m1800
http://oxgen.ge/product/pcr-fragment-purification/
0. Sample collection by
medical staff.
The patient sample is
taken with a swab,
packaged and labelled.
Patient data is stored with
a smartphone. QR code
labels are used throughout
the process.
1. Virus RNA extraction
using a simplified virus
lysis and RNA purification
protocol in 45 mins
without the need for a
centrifuge.
2. RNA amplification
is done with a isothermal
LAMP molecular biology
test kits in 30 mins using
a heatblock instead of a
thermocycler.
3. machine result readout
is ready after 1h with
minimal hands on time. QR
codes link the results to
the patient data.
Documentation happens by
smartphone photo and is
stored offline. Positive
results should be
confirmed with QPCR
32. UnternehmerTUM GmbH / 21.11.19
certified qPCR test protocol give
high quality results in 3h in the mobile lab
https://www.chaibio.com/openqpcr
0. Sample collection by
medical staff.
The patient sample is taken
with a swab, packaged and
labelled. Patient data is
stored with a smartphone.
1. Virus RNA extraction
virus lysis and RNA
purification using
established protocols with a
centrifuge in 45mins. QR
code labels are used
throughout the process.
2. QPCR RNA
amplification
is done with a reverse
transcription and QPCR
protocol and CE certified
molecular biology test kits
in 2h.
3. machine result readout
is ready after 2h. QR codes
link the results to the
patient data.
Documentation happens by
smartphone photo and is
stored offline.
33. UnternehmerTUM GmbH / 21.11.19
Micro.bio Lab.bio
Safely isolate and extract coronaviruses using a mobile
glovebox with negative pressure and autoclave to sterilize
contaminated materials. The station is designed for SARS-
Cov-2 RNA extraction. The station turns any tent, airport, or
hospital room or any place you need into a fully functional
biosafety level 2 facility.
The station provides a heat block, pipettes, a fridge and
freezer to keep your reagents fresh. Centrifuges and QPCR
machines (certified or research use only) can be ordered
additionally. Diagnostic results are documented by label
printer and the users smartphone.
Our Bio.Kitchen Mobile Lab covers all processes for
COVID-19 detection and safe sample handling
UnternehmerTUM GmbH / 21.11.19
34
https://www.chaibio.com/openqpcr http://www.glovebox-systemtechnik.de/GS-
Kunststoff-Glovebox.php
all pictures are for illustration only. The final product may differ.
34. Our Bio.Kitchen Mobile Corona Diagnostic Lab is a
ruggedized lab-in-a-box for pandemic response
▪ 3 mobile lab stations for installation in separated rooms for process
▪ Easily transported and operation - ready to use within ~24h, anywhere in EU
▪ Compatible to our molecular and microbiological lab tools
▪ Optimized equipment storage compatible with Eurocontainers
▪ Extendable table top area to a maximum of 7.2 m² (5 work spaces)
▪ On demand: mobile energy generator and voltage regulators
35. Bio.Kitchen Mobile Lab Properties
Durable and Secure
▪ Steel frame 25x25x2 mm welded and powder-coated
▪ TRESPA® Toplab Plus® 20 mm lab grade tabletop
▪ Lightweight, cleanable and robust eloxed aluminium AlucoBond
base cabinets
Practical
▪ All machines can be stored in “euro containers”
fitted into base cabinets
▪ Table addons such as garbage bins, hand soap holders, power-
plugs, lights and hygienic paper dispensers available
▪ Each stations tabletops can be extended by 850x700mm
extensions
Mobile
▪ Each station can be lifted into a transporter or up the
stairs by two people
▪ Using its robust wheels a station can be positioned anywhere
e.g. a wheelchair can access
Rapid and scalable production
First mobile lab prototype by October 2020
Production ready to scale from November 2020 on
36. UnternehmerTUM GmbH / 21.11.19
With 6 mobile lab stations Ghana could increase testing
rates equivalent to Germany’s
Assumptions:
● Staffing: 3 clinical laboratory technician working in 3 shifts 24/7
● 24 samples handled per hour, 20h diagnostic service, 4h maintenance
● a positive rate of less than 10%
● QPCR: hands on time 45 mins + 120 mins incubation time to result
● LAMP: hands on time 45 mins + 30 mins incubation time to result
capacity:
more than 500 diagnoses per station per 24h
38. 39
● 3x PPE –
personal
protective
equipment
● 2x
Pipettes+holde
rs
● Waste system
(autoclave)
● storage system
● Hand-hygiene
system
● Heat block
● qPCR
● digital
documentation
system (label
printer+smartp
hone)
● Table
centrifuge
● vortex
Lab Equipment
Mobile Lab
OxGEn certified
● hygiene kit
● sampling kit
● RNA extraction
kit
reagent supply
5 days life education
for Covid-19 sampling,
RNA Extraction, QPCR
and LAMP methods
course capacity up to
10 local students
Trainings
operate a Mobile Corona Laboratory with Instrumentation,
reagent supply and training
● Waste system
● Hand-hygiene
system
● PPE-personel
protective
equipment
● glovebox
● Storage
● 2x Fridge,
Freezer
● sink
Hie Biolab
● LAMP enzyme
production
Incas Diagnostics
● LAMP kits
manufacturing
Teaching of Teachers
for Covid-19
Diagnostics
LAMP kit Production
Methodology
Trainings reagent supply
41. Rüdiger
Trojok
Project Lead
trojok@
unternehmertum.de
Andreas
Jefimowicz
Tech Assistant
andreas.jefimowicz@
unternehmertum.de
Leo
Sprengel
Tech Assistant
sprengel@
unternehmertum.de
Prof. Gil Westmeyer
Holger Till
Florian Küster
florian.kuester@
maker-space.de
The Bio.Kitchen Team leads the project and is responsible for
project management in partnership with GIZ and HIVE Biolab
Dr Samuel Sakyi
Harry Akligoh + 3 TBD
drsakyi@gmail.com
Jasper Sablah
jasper.sablah@giz.de
Natascha Wisniewsky
natascha.wisniewsky@
maker-space.de
Steering Committee
Represenatives
GIZ | UnternehmerTUM
Susanne
Pettinger
Scientific Consultant
susannepettinger
@gmail.com
Vladimir
Baramidze
CEO Oxgen Ltd.
lado.baramidze
@gmail.com
