Participants of the workshop learn the necessary background information and techniques to diagnose Sars-CoV-2 using the mobile diagnostic laboratory. The laboratory is shipped ready to use with all devices, reagents, certificates, and protocols. After one day of preparation together with a local assistant, a five-day course is given where every step is carried out by each participant. Experts accompany the learning process with written teaching materials, video training, virtual live coaching, and short exams to verify the learned content.
3. Bio.Kitchen is an open community lab for tech-talents
5 workstations: micro- and molecular biology, chemistry, synthetic bio and lab automation
Rüdiger
Trojok
Lab Lead
Leo
Sprengel
Lab Assistant
Andreas
Jefimowicz
Lab Assistant
4.
5. Tools & Tech: rapid prototyping for agile life science
DNA Diagnostics Bentolab
Open Source Lab Hardware Open Source Pipetting
Robot
DNA Sequencer MinION
DNA Printer Kilobaser
Rapid Prototyping (digital)
Microfluidics
Cell Free Protein
Synthesis
Open Reagents
Collection
13. RT-QPCR is one globally accepted “Gold Standard” for testing on COVID-19. It is
expensive and time consuming. In addition handling in the lab is complex.
QPCR Thermocycler
14. RT-LAMP provides a cheaper and more reliable protocol compared to RT-
QPCR. Devices are cheaper, need less expertise. Time to result can be as low as
30min.
Heat block
reaction dependent dye for
visual readout
15. qPCR
LAMP
Sample
Sample preparation
(RNA extraction)
Sample preparation
(RNA extraction)
RNA amplification
RNA amplification
Analysis & report
RT-qPCR protocol
with results in 3,5
hours
Combination of qPCR and LAMP
15
0 0,5 1,0 1,5 2,0 2,5
Time
[hours]
3,0 3,5
Sample
Analysis & report
RUO LAMP quick test with
results in 2 hours without
thermocyclers
Test strategy:
Perform LAMP with all patient
samples.
Re-test LAMP-positive samples
with qPCR.
RT-LAMP = Reverse
Transcription Loop- Mediated
Isothermal Amplification.
17. 17
Mobile Sars-COV-2
Diagnostic Lab
Mobile Lab
Core Technology
Training
Support
qPCR & LAMP technology
and workflow for
Diagnostic
Easy to transport and
ready to use in 24 h
education courses to
train users
Supply of Consumables,
Reagents & online help
desk
18.
19. S2 Sample preparation room
S2 Patient
sampling
room
S1 Amplification
room
Patient
registration
& entrance
Patient
exit
S1 Reagent
preparation room
Patient
sample
Virus RNA
PCR & LAMP
samples
Patient sample RNA Extraction
RT-LAMP & RT-QPCR
Mastermix Preparation
RT-LAMP & RT-
QPCR
Amplification
Patient
sampling
Covid-19 Diagnostic process using open source
technology and reagents with the Bio.Kitchen Mobile Lab
20. Our Bio.Kitchen Mobile Corona Diagnostic Lab is a
ruggedized lab-in-a-box for pandemic response
▪ 4 mobile lab stations for installation in separated rooms for process
▪ Easily transported and operation - ready to use within ~24h, anywhere in EU
▪ Compatible to our molecular and microbiological lab tools
▪ Optimized equipment storage compatible with Eurocontainers
▪ Extendable table top area to a maximum of 7.2 m² (5 work spaces)
▪ On demand: mobile energy generator and voltage regulators
21. UnternehmerTUM GmbH / 21.11.19
With 6 mobile lab stations Ghana could increase testing
rates equivalent to Germany’s
Assumptions:
● Staffing: 3 clinical laboratory technician working in 3 shifts 24/7
● 24 samples handled per hour, 20h diagnostic service, 4h maintenance
● a positive rate of less than 10%
● QPCR: hands on time 45 mins + 120 mins incubation time to result
● LAMP: hands on time 45 mins + 30 mins incubation time to result
capacity:
more than 500 diagnoses per station per 24h
