Thermo Fisher Scientific Vanquish Duo Finland tour

1. The world leader in serving scienceProprietary & Confidential
Separate Your Science Tour
Oskari Aro (a.k.a. Tuomo Lukkari)
2019

2. 2 Proprietary & Confidential
Agenda
9:00 Thermo Scientific liquid chromatography solutions Oskari Aro
9:30 Smarter workflows with Chromeleon Merja Castrén
10:00 Charged Aerosol Detectors - What to try after UV and MS fail? Jan Pettersson
10:30 UV and DAD detectors - an in-depth overview Jan Pettersson
11:10 Coffee
11:30 Column selectivity Naghmeh Berglund
11:50 Enhanced bioseparations for peptide mapping and Mabs Naghmeh Berglund
12:10 Lunch
13:00 How solvent quality affects your analysis Jan Pettersson
13:20 Advanced detection with mass spectrometry Tuomo Lukkari
13:50 Carbohydrates analysis Jan Pettersson
14:30 Tips and tricks for HPLC Jan Pettersson
15:10 Chat with experts, Vanquish HPLC demo
16:00 Close

3. 3 Proprietary & Confidential
We enable our customers to make the world healthier, cleaner and safer.
A Mission We are Proud of

4. 4 Proprietary & Confidential
• ~$21 billion in revenues
• >$900 million spent on R&D
• >60,000 employees
• 50 countries
• 5 premier brands
The World Leader in Serving Science

5. 5 Proprietary & Confidential
Complementary Segments Create Competitive Advantage
Analytical Instruments Life Sciences Solutions
Laboratory Products and Services
Specialty Diagnostics
27%
17%
22%
34%Microbiology
Antimicrobial Susceptibility
Testing Solutions
QuantStudio
Dx R qPCR
Genetic
Sciences
BioPharma
Services
Laboratory
Chemicals
Bioproduction
Cell Culture
Reagents
Enterprise and
Instrument
Services
Biosciences
Life Science
Reagents
Pathology
Rotary
Microtome
Mass
Spectrometry
Fusion Lumos MS
Chromatography
Vanquish
UHPLC
Electron
Microscopy
Titan Krios TEM
Reproductive
Health
Microarrays
iCAP Triple Quad MS
Clinical
Diagnostics
PCT Biomarkers
Lab Equipment
TSX ULT Freezers
ImmunoDiagnostics
ImmunoCAP Allergy and
EliA Autoimmunity Tests
~$21B
Revenue
NOTE: Revenue and segment percentages
based on LTM through Q1 2017.
SeqStudio
CE System
Lab
Consumables
E1 ClipTip
Pipette System
Clinical Oncology
Next-Gen Sequencing
Transplant
Diagnostics
NXType High-Resolution
Genotyping
Chemical Analysis
Gemini Handheld Analyzer

6. 6 Proprietary & Confidential
Innovation in Orbitrap Technology
Next Generation GC-MS
Orbitrap™: Ten Years Young

7. 7 Proprietary & Confidential
Increased flexibility in liquid chromatography
Vanquish DUO UHPLC System
Chromeleon 7.2
SMART
Digest Kit
MAbPac Columns
Delivers exceptional performance, reliability
and reproducibility
Three workflows. Two flow paths. One
integrated UHPLC solution.
Complemented by kits, software and
columns
Thermo Scientific
ISQ EC Mass Spec

8. 8 Proprietary & Confidential
40+ years of innovation in Ion Chromatography
Thermo Scientific Dionex Ion Chromatography Portfolio: Peace of Mind Every Day
Thermo Scientific
Dionex Aquion
IC System
Thermo Scientific
Dionex Integrion
HPIC system
Thermo Scientific
Dionex ICS-4000
Thermo Scientific
Dionex ICS-6000 HPIC
Thermo Scientific
ISQ EC Mass Spec

9. 9 Proprietary & Confidential
Sample Prep, Kits and
Consumables
• MS Crosslinking reagents
• Capillary flow HPLC columns
• Metabolomics Standards Kits
Software
Instruments
Orbitrap HRAM MS
• Small molecule analysis
• Protein analysis
• Cloud Applications
• Spectral Libraries
Mass Spec Innovations in 2018
Triple Quadrupole LC-MS
Orbitrap Fusion™
Lumos™ MS – Options
TSQ Altis™ MS TSQ Quantis™ MS
Q Exactive™ HF-X
LC-MS
Proteome Discoverer™
Software
Compound Discoverer™
Software
Biocrates
AbsoluteIDQ
p400HR Kit
DSSO and DSBU
MS-Cleavable
Linkers
EASY-Spray™
PepMap
150µm Column
• UVPD
• 1M
• APD
Food and
Beverage
Environmental
Translational
Research/
Precision
Medicine
Life Science
Research
“omics”
Biopharmaceuticals
Exactive™ GC Orbitrap™
GC-MS
TSQ 9000 GC-MSISQ 7000 GC-MS

10. 10 Proprietary & Confidential
NEW! Thermo Scientific ISQ EC/EM Single Quadrupole Mass Spectrometers
• Mass range 10-1250 m/z
• HESI only
• Perfect for IC-MS or LC-MS
• For ion chromatography (IC-MS) and liquid chromatography (LC-MS)
• Built for routine analysis and long-term signal stability
• Controlled by Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)
Thermo Scientific™ ISQ™ EC
Single Quadruple Mass Spectrometer
• Mass range 10-2000 m/z
• HESI and APCI Options
• Perfect for LC-MS
Thermo Scientific™ ISQ™ EM
Single Quadruple Mass Spectrometer

11. 12 Proprietary & Confidential
Sample Preparation ProductsSample Handling Products
Liquid Chromatography Columns Gas Chromatography Products
Chromatography Consumables Product Overview
Smart Digest kits
Bio-LC columns
Vials, Inserts
& closures
Accucore
core-shell
columns
Small molecule columns
Low-flow columns
GC columns
GC accessories
GC reagents
Gas
filters
Micro plates
& mats Virtuoso Vial
Identification
System
Sola SPE
cartridges
SPE cartridges
Sample filtration

12. 13 Proprietary & Confidential
Automated, photometric testing for water, wine, beer, juice, food and enzymes
Discrete Industrial Analyzer
Common Water Quality Tests
Phosphate
Chloride
Nitrate
Nitrite
Ammonia
Hardness and Alkalinity
Fluoride
Ready for immediate analysis; no reagent priming, no
method changeover time
Gallery Discrete Analyzers have very fast sample throughput
from 200 – 350 tests/hour
Achieves low detection levels (to ppb levels) using proven
photometric technology

13. 14 Proprietary & Confidential
Vanquish UHPLC

14. 15
620 bar
Thermo ScientificTM
UltiMateTM 3000 Basic
Automated
•Highly economic &
reliable
Up to 1000 bar 1500 bar
Vanquish Horizon
•High pressure binary
solvent mixing
•Industry-leading precision
and accuracy
•Two thermostatting modes
•Unmatched detection
sensitivity
•Biocompatible
•Integration of multiple
detection technologies
Vanquish Flex
•High pressure binary
and low pressure
quaternary solvent
mixing options
•Two thermostatting
modes
•Biocompatible
•Integration of multiple
detection technologies
Thermo ScientificTM
UltiMateTM 3000 SD
•Workhorse for standard
HPLC applications
Thermo ScientificTM
UltiMateTM 3000
RS/BioRS
•Specialty workflow
support
•Binary and quaternary
UHPLC systems
800-1200 bar
Thermo ScientificTM
UltiMateTM 3000
RSLCnano
Thermo Scientific™
EASY-nLC™ 1200
•UHPLC systems for
Nano/Cap/Micro range
•Ideal front-end for
proteomics applications
HPLC systems UHPLC systems
Routine analysis Low-flow
Flexibility, more performance and method
development
Research & HT
Thermo Scientific HPLC Portfolio

15. 16
Vanquish Platform
Thermo Scientific™ Vanquish™ Flex
Quaternary system
• 1000 bar Quaternary System
• Medium Gradient Delay Volume
UHPLC for resolution or method development
Highest performance for best separation or
throughput
Thermo Scientific™ Vanquish™ Horizon
system
• 1500 bar Binary System
• Ultra low Gradient Delay Volume
Vanquish Systems – Comparison
Thermo Scientific™ Vanquish™ Flex
Binary system
• 1000 bar Binary System
• Low Gradient Delay Volume
Throughput for targeted UHPLC separations
• Improved retention time precision
• Biocompatible by default
• Increased sample capacity
• Active and passive pre-heating
• Many detection options
• Platform-inherent robustness & maintenance features

16. 17
Column efficiency improvement - Van Deemter plots
5m
1.9m
u (mm/s)
H(m)
5
10
15
0 61 2 3 4 5
3m
IncreasingColumnEfficiency(N)
pd
N
1

L
N
H 
Increasing Flow Rate
Faster runs with better efficiency and
without compromise in performance!

17. 18
Vanquish Horizon Delivers Method Speed-Up
Standard Method Sequence Coverage
100%
100%
100%
100%
100%
Acclaim 120 C18 column, 2.1 x 250 mm, 2 - 45% ACN + 0.1% FA, 60℃.
5 10 15 20 25 [min]
Time (min)
14.64
20.23
10.592.73
10.78
14.52
7.99
2.70
6.97 9.43
5.20
1.86
4.45
6.02
3.35
1.13
3.12
4.12
2.43
1.08
30 min gradient
0.4 mL min-1
600 bar
20 min gradient
0.4 mL min-1
600 bar
13 min gradient
0.6 mL min-1
850 bar
8 min gradient
1.0 mL min-1
1150 bar
5 min gradient
1.1 mL min-1
1300 bar
• Vanquish used for high
throughput peptide mapping
• Gradient time reduced from 3 to
5 min with constant 100%
sequence coverage
• Thermo Scientific™ Q
Exactive™ HF hybrid
quadrupole-Orbitrap mass
spectrometer delivers enough
data points per
chromatographic peak
• Vanquish is able to speed up
peptide mapping significantly

18. 19 Proprietary & Confidential
Vanquish – A Completely New Developed Autosampler
• Metering device replaces syringe
• Fine aspiration drive for ultra precise dosage
• Sample pre-compression for outstanding
retention time precision and column lifetime
• Newly developed ceramic valve
• Advanced air-to-air cooling
• Extended sample capacity
• 4 sample racks and/or well plates
• Optimized fluidic design

19. 20 Proprietary & Confidential
Experimental Results – Pre-Compression
Column: Hypersil Gold, 1.9 µm, 2.1 × 100 mm
System: Vanquish System
Mobile Phase: A–water
B–acetonitrile
Gradient:
Flow rate: 260 L/min
Pressure: 300 bar (max.)
Comp. Vol: 0 µL and 3 µL
Temperature: 25 ºC
Injection: 1 µL
Detection: pump pressure sensor
Time
[min]
% A %B
0 60 40
0.4 60 40
4.0 0 100
4.8 0 100
4.9 60 40
10.0 60 40
w/o pre-
compression
w/ pre-
compression
Inject command at 0.1 min to track the pressure drop
 isocratic step from 0 to 0.4 min (cycle completed)
 No pressure drop during injection
Why is this important?
• Reduced physical stress on column longer lifetime w/o
performance loss less work
• More stable flow in early gradient forming  better retention time
reproducibility  better results  more confidence

20. 21
Column Lifetime Stability Evaluation
injection
#1953
#1213
#404
Vanquish UHPLC Family:
Intelligent Smart Inject feature
with sample pre-compression
enables excellent column
performance up to 1950 injections!
Vanquish Horizon &
Vanquish Flex
UHPLC Systems
MAbPac SEC-1, 4x300 mm, 0.3 mL/min
On Column
Injection #
Retention
Time (min)
Monomer
Relative Peak
Area (%)
Monomer Peak
Width @ 50%
Height (min)
Asymmetry
(EP)
Theoretical
Plates (EP)
25 9.554 96.96 0.237 0.92 9032
404 9.558 97.21 0.240 0.92 8797
665 9.558 96.96 0.241 0.93 8736
1213 9.567 97.27 0.243 0.89 8604
1551 9.544 96.99 0.244 0.88 8460
1953 9.524 96.30 0.255 0.89 7737
85% initial column efficiency following 1866 mAb
injections (1953 on-column injections)

21. 22 Proprietary & Confidential
VAS – Injection Valve
• Innovative new valve design by Thermo Scientific
• Compact valve design for optimized flow path
• Bio-compatible valve
• Stator and rotor made of ceramics
• Long-life DLC coating
• Valve is available as an entire assembly
• Valve is made for Viper fittings
• Needle seat is directly installed on the injection valve
• Integrated needle seat connection to the valve
• Reduces extra column volume
Port Connected Component
1 Inlet capillary from the pump
2 Outlet capillary to the column
3 Needle seat (pre-installed)
4 Wash port waste (pre-installed)
6 Metering device (pre-installed)

22. 23 Proprietary & Confidential
• Bioinert 1/32″ capillary
• Finger tight, correct fit every time!
• Universal
• Virtually zero-dead volume connections
• Ease of use, one-piece design
PEEK Seal
Removable knurl
Adapter (no ferrule!)
Finger Tight Screw Bioinert
1/32″ capillary
Biocompatible Viper™ Fingertight Fitting System

23. 24 Proprietary & Confidential
Vanquish – Column Compartment
• High temperature accuracy and precision to host 2 columns
• Temperature range 5 – 120˚C
• Support of forced air and still air
operating modes
• Actively controlled pre-heater
• Upgradeable with up to two
switching valves
• Expandable for multiple columns
and temperature zones

24. 25
Three Workflows. Two Flow Paths. One Integrated UHPLC Solution.
Dual LC
• A two channel LC system
with standard footprint
Tandem LC/LC-MS
• System for efficient
detector utilization
Inverse gradient
• System for advanced
quantification capabilities