5. HOW TO EXTRACT NUCLEIC ACIDS?
Manual Extractions Automated Extractions
90%
10%
Manual
Automation
6. MOLECULAR DIAGNOSTIC MARKET
---A Rapid Growth Market of IVD
Rapidly Increasing of Molecular Market
Data source: DARK Daily Laboratory and Pathology News
Data source: Frost & Sullivan
• Economic downturn and saturation
• Unstable reimbursement regulations
• For developed countries
• Increasing infectious diseases
• Rising incidences of cancer
• Genetic disorders
• Blood screening
Year 2013-2019: CAGR: 11.1%
7. MOLECULAR DIAGNOSTIC MARKET
16%
15%
5%
64%
Fish
Real Time PCR
Microarray
PCR
• Dominant nature & acceptance
of PCR & Real Time PCR over
other technologies, represents
almost 80% of the molecular
diagnostic market.
8. Nucleotide:
ATP, CTP,TTP,GTP
Primers
Template DNA
The Polymerase Chain Reaction (PCR) is a technology in molecular
biology used to amplify a single copy or a few copies of a piece
of DNA across several orders of magnitude, generating thousands to
millions of copies of a particular DNA sequence.
POLYMERASE CHAIN REACTION (PCR)
14. INTENDED USE OF SOLMAG
®
Magnetic Bead Separation:
Bench-top automated system for quick purification of nucleic acids from bio-
specimens using magnetic bead separation technology.
Liquid Handling System:
Liquid handling automation, using pre-filled reagent cartridges
For research and in vitro diagnosis use
15. CORE TECHNOLOGIES
The Solis-Charger
®
Technology
The Solis-ChargerR Technology is a novel magnetic bead-based technology
that provides a switchable surface charge dependent on the pH and salt
concentration of the surrounding buffer to facilitate nucleic acid purification.
In low pH conditions, the magnetic beads have a positive charge that binds
the negatively charged nucleic acid backbone (see figure1 below). Proteins
and other contaminants are not bound and are simply washed away in an
aqueous wash buffer. To elute nucleic acids, the charge on the surface of the
bead is neutralized by raising the pH to 8.0 using a low salt elution buffer (see
figure below). Purified nucleic acid elutes instantly into this elution buffer, and
is ready for use in downstream applications.
16. SOLMAG
®
12 SPECIFICATION
Automated Nucleic Acid Purification System
SolMag
®
12:
Compact Bench-top Instrument
Process 1-12 samples simultaneously
7 inches touch screen
Light: Total weight less than 42 Kg
Fast: Up to 12 results in 35 minutes
Precise liquid volume and temperature control
Extremely easy to operate and maintain
SolMag
®
24:
The SolMag®24 provides all the advantages of the
SolMag®12, and increases the throughput to 24
samples per run
17. SOLMAG
®
12 SPECIFICATIONS
Light as feather:
SolMag
®
12 weights only
42kgw, which is 50% lighter in
average than other similar
throughputs liquid handling
extractor in the market.
MagNaPure Compact: 60Kg
Qiagen EZ1 Advanced XL: 48Kg
MagCore HF16: 70Kg
SolMag
®
12
Part No. SVP0101
Dimensions (W x D x H) 637mm x 462mm x 540mm
Weight 42kgw
Interface 7” LCD touch-screen
Processing time 20 – 45mins
Throughput 1-12 Samples per run
Temperature
Uniformity
±0.5℃
Liquid Extraction
Volume Error
Less than 5％
Sample Volume 50ul – 3000ul
Elution Volume 30ul – 200ul
Power Input power :
90 ~ 132V&180 ~ 264Vby switch
50/60Hz
Out power :5V60A
Certification CE、IVD、FDA
18. UNIQUE SPECIFICATIONS
7” Touch Screen:
Optimized built-in protocol covers six major applications such as clinical
diagnostic, forensic science, food safety and CFC.
Built-in Template:
Up to three preferable
templates can be set up by
the users for performing the
same routine protocol
daily, which can also
reduce the risk from human
operational error.
19. SOLMAG ISOLATION KITS
Ready-to-use pre-filled, Reagent Cartridge:
The SolMag
®
Nucleic Acid Isolation kits contain all reagents and
disposables needed for 48 nucleic acid isolation reactions.
SolMag
®
NA Isolation Kits
1. Blood/Tissue Nucleic Acid Isolation Kit
2. Virus Nucleic Acid Isolation Kit
3. Pathogen Nucleic Acid Isolation Kit
4. Food Safety Nucleic Acid Isolation Kit
5. Forensic Science DNA Isolation Kit
6. Circulation Nucleic Acid Isolation Kit
7. Virus Nucleic Acid Isolation Kit - Large Volume
• Use of prefilled, film-sealed
reagent cartridges
• No manual adding chemicals
or reagent buffers required
• Auto piercing after the
purification protocol runs
20. INTEGRATED ADVANTAGES
Reliable
Advanced magnetic separation technology
High collection rate of Magnetic particles (≥98%) and
sensitivity(≥98% of 100 copies/mL)
High purity
Consistent heating temperature: ≤1.5% well to well CV
Efficient
Short set up time.
extract 24 specimens within 30~40 minutes
User-friendly
UV lamp, effective to avoid contamination
Exceptionally stable & quiet during whole extraction
process.
Human-friendly touch screen user interface
21. TARGET MARKETS
•Pregnant women: NIFTY
•Blood donators: Screening such as HBV, HCV
etc. from blood
Screening
•Cancer patients: Tumor target gene mutation
detect for drug use
•Infectious disease patients: Drug resistance
detection such as HBV YMDD mutation detect
Monitor
•Vaccine acceptors: Genotyping detection for
virus before vaccine injection, such as HPV.
•Patients with chronic kidney disease or liver
disease, HBV, HCV…: Blood test.
Prevention
•PCR for target gene accumulation
•Sequencing for mutation & methylation
analysis
•Further biological research such as
transcription, plasmid composing, RNAi etc.
Research
Hospital;
Blood stations
Pharmacies;
Dialysis center
Community
Health Centers ;
CDC
Biology Labs:
Academies
Research
Institutes
22. TARGET CUSTOMERS
Region:
• Undeveloped, Under-Developed Countries: High infectious diseases rate
(STD, HBV, HCV, HPV)
• Developed Countries: Gene Therapy, Sequencing (Micro Array, NGS)
Potential Customers:
• Hospital & CDC
• Basic biological
research
• Blood bank, DNA bank
• Forensic lab
• Food inspection lab
• GMO lab
23. COMPETITIONS- Magnetic Pillar/Rod System
Advantage
Compact Size
Relative low cost on instrument and kits
Disadvantage
Easy to have magnetic bead residues in eluted products
Limited or fixed sample/ elute volume handling choice
No liquid handling system, extremely low flexibility for downstream
automated integration.
Semi-Automation: tedious manual set up procedure required, kills time and
cause humor errors.
Ex:
Maxwell (Promega)
KingFisher (Thermo Fisher)
Tan Beads (Taiwan)
Vibrance (Acon, China)
TianLong (China)
24. COMPETITIONS- Liquid Handling System
Advantage
Fully walk away automation: Liquid Handling ensures the highest possible
automated integration. (NA extraction PCR set up PCR
detection, sample in to result out solution in future)
Enable to handle a wide range of volumes on starting material.
Extra manual steps during before or during extraction are not required.
Disadvantage
Compare with Magnetic Pillar system, size of instrument is bigger and
heavier.
Higher instrument cost
Ex:
MagNa Pure Compact (Roche)
EZ1 Advanced (Qiagen)
iPrep (Invitrogen)
(All Manufactured by PSS, Japan)
SOLMAG (SOLISON)
MagCore (RBC)
25. COMPETITION COMPARISON
Instruments
Solison
(SolMag12)
Maxwell
(Promega)
Qiagen
Qia Symphony
MagNaPure
(Compact)
Mechanical Design Liquid Handling
Magnetic
Pillar/Rod
Working Station Liquid Handling
Open/Closed Platform Closed Open Closed Closed
Technology (magnetic and/or
silica/filtration (vacuum) extraction)
Magnetic beads Magnetic beads Magnetic beads Magnetic beads
Input specimen volume range,
min-max(ul)
50ul-3000ul 400ul 100-1000ul 200-400ul
Output volume, min-max(ul) 30-200ul N/A 20-150ul 60-200ul
Throughput 12 16 1-96 1-8
Estimated cost/ Instrument US$10,000 $17,000 US$50,000 US$30,000
Estimated cost/specimen $2.0 $5.5 $5.6 US$7
