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Ppt spectroscopy
1. TO
DEVELOP AND VALIDATE
DERIVATIVE SPECTROSCOPIC
METHOD FOR ESTIMATION OF
SERRATIOPEPTDASE AND DICLOFENAC SODIUM
IN BULK AND IN TABLET
BY
ROHIT BIYANI
GUIDE
MRS. MANSI. J. WAGARIKAR
2. To develop Derivative Spectroscopic method
for UV visible spectrophotometric qualitative
and quantitative estimation of
Serratiopeptidase and Diclofenac Sodium in
bulk and in tablet.
To validate the developed analytical method
for Linearity, Accuracy and Precision as per the
ICH guidelines.
4. Instrument: The instrument used is
SCHIMADZU UV-1700
Solvents: All the chemicals used in this work
were of AR grade.
Drug: Pure drug sample was obtained from
Alkem Pharmaceuticals limited.
5. Principle
Beer-Lamberts Law
Different methods for multicomponent analysis
Simultaneous Equation Method
Area Under Curve Method
Derivative Spectroscopic Method
Multicomponent mode Method
Q-Analysis Method
6. Instruments for measuring the absorption of
Ultraviolet and Visible radiation are made up
of Following parts-
1. Radiation Source
2. Wavelength selectors
3. Sample containers
4. Radiation transducers
5. Signal processors and Read-out device.
9. PRINCIPLE
Derivative spectrophotometry involves the conversion of a normal spectrum
(fundamental,zero ,D spectrum) to its first ,second or higher derivative spectrum by
differentiating absorbance of a sample w.r.t wavelength λ for high accuracy
From fig above,
[A]=f(λ):zero order
[dA/d λ]=f(λ):first order
[d2A/d λ2 ]=f(λ):second order.
The strong positive &negative bands with max and min at same wavelength of an
absorption band as inflection point in absorbance band governs the odd (first and third )
of an absorption band as inflection point in absorbance band governs the odd (first and
third )derivative spectrum whereas the strong positive &negative band with min or max
at same wavelength as λ max of absorbance band governs the even (second and fourth)
derivative spectrum.
No of bands=Derivative order+1
The amplitude (D) is Directly proportional to the conc of analyte provided beer law is
obeyed by D0spectrum.
In First Order Derivative spectroscopy ,zero crossing point for both drugs is found and
wavelength are selected in a manner such tht at zero crossing of one drug .the other drug
should show substantial absorbance.
10.
11. Preparation of stock solution
Preparation of standard solution
Preparation of sample solution: BULK and
TABLET
21. Method Drug Standard deviation RSD COV
Bulk Tablet Bulk Tablet Bulk Tablet
derivative
SER
1.41231 2.69733 0.029161 0.04680 2.9161074 4.68078
DIC
0.32548 3.05189 0.095560 0.18384 9.5560775 0.18384
22. UV Visible spectrophotometric derivative spectroscopy
Method was developed for qualitative and quantitative
estimation of Serratiopeptidase and Diclofenac Sodium
in tablet formulation and in bulk.
The developed method was found to be simple, rapid
and precise for routine estimation of Serratiopeptidase
and Diclofenac Sodium in tablet dosage form. The
standard deviation was satisfactorily low indicating
precision of methods. Method was found to be
accurate, precise.
Thus, developed UV Visible spectrophotometric
method can be satisfactorily used for determining the
content of Serratiopeptidase and Diclofenac Sodium in
bulk and tablet dosage form.
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