This document discusses potential future treatments for diabetes using stem cells and gene therapy. It outlines the limitations of using mature B-cells as a treatment source and describes the developmental stages of beta cells. Gene therapy involves preparing genetic material and a delivery system. Non-embryonic stem cell sources include adult bone marrow, umbilical cord cells, and amniotic fluid cells. Induced pluripotent stem cells can be created by reprogramming adult cells back to a stem cell state. Recent research focuses on using mesenchymal stem cells for their anti-inflammatory and immune-suppressive properties to protect pancreatic beta cells from immune destruction in type 1 diabetes.

1. ِMEDICINE OF THE FUTURE
STEM CELLS AND GENE THERAPY
IN DIABETES
by
Prof Morsi Arab
University of Alexandria

2. The mature B-cells as a source (B-cell
transplantation ) is not satisfactory
because they are :
-Limited in number,
- have very limited proliferation capacity.
- and stimulating their growth by growth factors
diminishes their secretory power.

8. :
Stages of Beta cell development
and differentiation
- Fetal Entoderm. cell ( high growth
potentiality)

- Panc. Duct epith. Cell

- Committed Endoc. precursor cell

- Growth Stimulated B-cell

- Mature B- cell (V. low division capacity)

10. GENE THERAPY and STEM CELLS
in DIABETES

11. Gene Therapy is achieved in
two steps
i. Prepare The Genetic Material
+
ii. Delivery system

15. Non Embryonic Sources of Stem Cells
Adult Bone Marrow
Umbilical cord Stem Cells
Amniotic Fluid Stem Cells
Adult Mesenchymal Stem Cells*….etc

16. REPROGRAMMING ADULT
CELLS BACK TO THE STEM
CELL STATE
i.e. Inducing Pluropotency ))

17. The production of induced Pluripotent
Stem(Cells ( iPSC
By genetic reprogramming of mature adult
cells to go back to the pluripotent state
(adding genes + transcription factors (TF)

22. New Developments in Stem
Cells Research in Diabetes 2015

23. - In type 1 Diabetes pancreatic cell
destruction results from inflammation + immune
reaction
- Adult Mesenchymal Stem Cells ( MSCs)
have potent anti- inflammatory and
immune suppressive power .

24. “Trafficking of Adult Mesenchyme Stem Cells and
using Adhesive Molecules towards the Threatened
Pancreatic β cells
saves them from immune destruction”

25. Thank You