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PRIMARY CELLS
VS.
CELL LINES
Creative Bioarray
A Division Of Creative Dynamics Inc
WHAT IS
PRIMARY CELL?
Primary cells are cells taken directly from living
tissue and established for growth in vitro.
These cells are retaining of the main natural
functional of the tissue.
Generally, they retain normal morphology, cellular
function, growth characteristics, cellular markers,
signaling and genetic integrity when propagated in
culture.
Creative Bioarray
A Division Of Creative Dynamics
Inc
CELL LINES PROFILE
➤ Cells that have been continually
passaged over a long period of time.
➤ Cell lines can be finite or continuous.
➤ They are preferably used for convenience
as they are easy to handle and widely
published
➤ However, these cells less preferred as a
biologically relevant option, since they
have lost the true characteristics of the
original tissue from which they were
isolated.
Creative BioarrayA Division Of Creative Dynamics
Inc
The Major Differences Between Primary Cells and Cell Lines
Primary Cells Cell Lines
Cell Division In Vitro Limited Indefinite
More Closely Mimic
In Vivo Conditions
Yes No
Chromosomal Aberrations Minimal Several
Provide High Quality Data on
Interactive Pathways,
Processes, and Function
Retain functional enzymatic and
signaling pathways of parent tissue
Potentially provide misleading data
due to aberrant enzymatic or
signaling pathway activities
Authentication required
before use
No, if bought commercially
Yes, mandated by many government
institutes and scientific journals
Care and Maintenance of
the Cells
Can be complex and difficult,
especially in maintaining
differentiated state
Easy to maintain or propagate
“
Creative BioarrayA Division Of Creative Dynamics
Inc
With the development of technology, the primary cell culture has changed.
New tools, new approach.
Primary cell types: They’re the real deal!
Knowing the “how &
where” of the cells and
support materials is
crucial.
Using a well-characterized,
terminally differentiated cell
type can deliver confidence
and assurance in data
generated in vitro.
Entrusting biomedical
research to verified or
more thoroughly
characterized cell culture
tools will deliver the most
meaningful and
trustworthy experimental
results short of performing
Cost to use, maintenance
in culture, and other
unique challenges
associated with primary
cells should be considered.
Some of the applications where primary cells are being
used
➤ Cellular pathway analysis
➤ ADME & toxicology studies
➤ Regenerative medicine
➤ Adult stem cells
➤ Metabolic disease research
➤ Comparative studies vs. transformed cell lines
➤ HTS/HCA screening
➤ Other platforms
Commercial primary cell providers
——Creative Bioarray
➤ 35 human cell systems with over 160 different cell types
➤ Over 13 types of other animals & A selection of primary
cells of animals for comparative testing
➤ Also provide related services to meet specific client
requirements
➤ Strict quality control and supply whole information with
each of our products
Creative Bioarray
A Division Of Creative Dynamics Inc
USA
 
 COMPANY: CREATIVE BIOARRAY
 PHONE: 1-631-626-9181
 FAX: 1-631-614-7828
 ADDRESS: 45-1 RAMSEY ROAD, SHIRLEY,
NY 11967, USA
 EMAIL: INFO@CREATIVE-
BIOARRAY.COM
 
 EUROPE
 
 COMPANY: CREATIVE BIOARRAY
 PHONE: 44-207-097-1828
 EMAIL: INFO@CREATIVE-
BIOARRAY.COM
Looking forward to cooperating with you!

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Primary Cells vs Cell Lines: Key Differences

  • 1. PRIMARY CELLS VS. CELL LINES Creative Bioarray A Division Of Creative Dynamics Inc
  • 2. WHAT IS PRIMARY CELL? Primary cells are cells taken directly from living tissue and established for growth in vitro. These cells are retaining of the main natural functional of the tissue. Generally, they retain normal morphology, cellular function, growth characteristics, cellular markers, signaling and genetic integrity when propagated in culture. Creative Bioarray A Division Of Creative Dynamics Inc
  • 3. CELL LINES PROFILE ➤ Cells that have been continually passaged over a long period of time. ➤ Cell lines can be finite or continuous. ➤ They are preferably used for convenience as they are easy to handle and widely published ➤ However, these cells less preferred as a biologically relevant option, since they have lost the true characteristics of the original tissue from which they were isolated. Creative BioarrayA Division Of Creative Dynamics Inc
  • 4. The Major Differences Between Primary Cells and Cell Lines Primary Cells Cell Lines Cell Division In Vitro Limited Indefinite More Closely Mimic In Vivo Conditions Yes No Chromosomal Aberrations Minimal Several Provide High Quality Data on Interactive Pathways, Processes, and Function Retain functional enzymatic and signaling pathways of parent tissue Potentially provide misleading data due to aberrant enzymatic or signaling pathway activities Authentication required before use No, if bought commercially Yes, mandated by many government institutes and scientific journals Care and Maintenance of the Cells Can be complex and difficult, especially in maintaining differentiated state Easy to maintain or propagate
  • 5. “ Creative BioarrayA Division Of Creative Dynamics Inc With the development of technology, the primary cell culture has changed. New tools, new approach.
  • 6. Primary cell types: They’re the real deal! Knowing the “how & where” of the cells and support materials is crucial. Using a well-characterized, terminally differentiated cell type can deliver confidence and assurance in data generated in vitro. Entrusting biomedical research to verified or more thoroughly characterized cell culture tools will deliver the most meaningful and trustworthy experimental results short of performing Cost to use, maintenance in culture, and other unique challenges associated with primary cells should be considered.
  • 7. Some of the applications where primary cells are being used ➤ Cellular pathway analysis ➤ ADME & toxicology studies ➤ Regenerative medicine ➤ Adult stem cells ➤ Metabolic disease research ➤ Comparative studies vs. transformed cell lines ➤ HTS/HCA screening ➤ Other platforms
  • 8. Commercial primary cell providers ——Creative Bioarray ➤ 35 human cell systems with over 160 different cell types ➤ Over 13 types of other animals & A selection of primary cells of animals for comparative testing ➤ Also provide related services to meet specific client requirements ➤ Strict quality control and supply whole information with each of our products Creative Bioarray A Division Of Creative Dynamics Inc
  • 9. USA    COMPANY: CREATIVE BIOARRAY  PHONE: 1-631-626-9181  FAX: 1-631-614-7828  ADDRESS: 45-1 RAMSEY ROAD, SHIRLEY, NY 11967, USA  EMAIL: INFO@CREATIVE- BIOARRAY.COM    EUROPE    COMPANY: CREATIVE BIOARRAY  PHONE: 44-207-097-1828  EMAIL: INFO@CREATIVE- BIOARRAY.COM Looking forward to cooperating with you!