2. -: Topics to be covered :-
• What is Transpiration
• Experiments to demonstrate Transpiration
• Kinds of Transpiration
• Mechanism of stomatal Transpiration
• Factor affecting rate of Transpiration
3. What Is Transpiration :-
Large quantities of water are absorbed by the plants from the
soil but a small amount of it is utilized.
The excess amount of water is lost from the aerial parts of the
plants in the form of vapour is known as Transpiration.
Transpiration is the vital physiological process in which the
water is lost from the aerial parts of the plant.
Transpiration was first worked out by Stephen Hales, an English
clergyman.
He first gave the idea of loss of water in the form of vapour,
which is still belived.
4. Experiments to demonstrate transpiration
Some important experiments have done to demonstrate and measure the rate of
transpiration.
Some of them are
5. Types Of Transpiration
3 types of Transpiration is found these are;
i. Stomatal transpiration
ii. Cuticular transpiration
iii. Lenticular transpiration
Stomatal Transpiration:-
The transpiration process which occurs through stomata is called Stomatal
Transpiration. The stomata are found numerously on the leaf surface.
6. Cuticular Transpiration :-
cuticle is impervious to water still it looses some water i.e. about 10%
of total transpiration.
Lenticular transpiration :-
Some of the water may be lost by the woody stem through lenticels
which is called as Lenticular transpiration .
7. Mechanism of transpiration :-
The stomatal transpiration occurs due to the stomatal movement i.e. the mechanism
of opening and closing of stomata.
Structure of stomata :-
stomata are small minute pore like structure present in surface of leaf
and are guarded by two guard cells.
• According to the number of distribution of Stomata, they are of 3 types i.e.
epistomatal, hypostomatal, amphistomatal respectively.
• Guardcell contain chloroplast, nucleus, contractile vacule.
• Subsidary cell doesnot contain chloroplast .
8. Opening and closing of Stomata :-
The mechanism of oprning and closing of stomata involves 2 steps;
a) Starch sugar Interconversion Theory
b) ATP-driven proton- K+ Exchange pump
i. Starch sugar Interconversion Theory :-
• According to Liyord (1908);
Starch Sugar
• According to Sayre (1948)
Starch Sugar (at certain pH )
• According to Yin and Tung
(close) Starch glucose-1-phosphate ( open)
9. • According to steward(1964) the starch during day time at pH 7.0
converted to glucose-1-phosphate, which causes the stomata open by
increasing the OP of guard cell.
• During night the glucose-1-phosphate converted into starch, which
causes the stomata close by decreasing the OP of guard cell.
10. ii. ATP-driven proton- K+ Exchange pump :-
During day:-
6𝐶𝑂2 + 12 H20 𝐶6 𝐻12 𝑂6 + 6𝑂2
𝐶6 𝐻12 𝑂6 Malic Acid
Malic Acid Malate ion + 𝐻+
During night :-
• As 𝐶𝑂2 is not utilized by photosynthesis during night hence;
6𝐶𝑂2 + 12 H20 Carbonic acid
• Then it forms ABA (Abscisic acid).
• The 𝑘+ are transported back to the guard cell.
12. Factor affecting rate of Transpiration :-
Two important factors are responsible;
a) External Factor
b) Internal Factor
I. External Factor :-
• Atmospheric Humidity is inversely proportional to rate of transpiration.
• Temperature is directly proportional to rate of transpiration.
• Light intensity is directly proportional to rate of transpiration.
• Wind velocity is directly proportional to rate of transpiration.
• Carbon dioxide concentration is inversely proportional to rate of transpiration.
II. Internal Factor :- The factors like structure of leaf area of transpiring surface, number of
stomata, orientation of leaf are included in the category.