Microbial culture collections are important for the development of scientific research and industrial exploitation. In order to provide easier access to the collection, the list of microbial cultures should be made available online. A survey on the CCINFO, powered by the WDCM reveals that 60 collections from Thailand registered into CCINFO database during 1983-2013, and only 2 collections: BCC (BIOTEC culture collection) and TISTR provide the online access directly to their microbe information. In addition, at WDCM, 48 from 60 Thai collections are affiliate at universities, but their information in CCINFO is totally outdated. Likewise, only BCC and TISTR published their information in the GCM with the total number of 4,948 and 1,032 strains respectively. It is observed that some data of 4 collections including BCC, TISTR, DOA and DMST can be online accessed through the Thailand Network on Culture Collections (TNCC) website. Established since 2000, TNCC is the first data network of the aforementioned four culture collections in Thailand. The main activity of TNCC is to integrate information from four members and disseminate microbial information to public. To transfer their data into an integrated database, TNCC members can submit the data file in excel or enter each record into a web application. Now there are only 5,550 strains available at TNCC website that is far from what registered at CCINFO, WDCM. The lack of up-to-date information is the main problem at both WDCM and TNCC, although WDCM and TNCC focus at different network levels: individual collections and country service collections. The data and system integration across levels of network might help to co-create the integrated information for understanding the microbial diversity, and integrated services for connecting to industrial networks.
From the network integration perspective, BIOTEC has launched Thailand Bioresource Research Center (TBRC) initiative in 2012 to deliver the co-creating services to bridge a gap between academic research and industry. TBRC aims to (1) leverage the network strength of service collections (2) create a research network based biological resource center and (3) establish a common technology infrastructure to provide open standards and interoperable information systems across the network levels. TBRC network applies the concept for cooperative work based on a hosted solution using a shared server. The collection registered at TBRC will get a software called iCollect to be used in managing the data from their preservation activities, update the list of microbial cultures to TBRC online catalogs and retrieve orders from TBRC via SOAP-based Web services. Alternatively, the members can manage, process their data and transmit back and forth through TBRC website. Integrated data and information will then be curated, annotated and categorized using data mining techniques and made accessible through TBRC online distribution service, www.tbrcnetwork.org.
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TBRC: an online networking platform for biological collections in Thailand
1. TBRC:
an
online
networking
pla3orm
for
biological
collec8ons
in
Thailand
9/11/13 Copyright
2012-‐2013
BIOTEC
1
TBRC: a National BRC
Supawadee Ingsriswang
Bioresources Technology Research Unit,
National Center for Genetic Engineering and
Biotechnology (BIOTEC), Thailand
http://www.biotec.or.th/btu
Thailand Bioresource Research Center
(TBRC), Thailand
http://www.tbrcnetwork.org
2. 9/11/13Copyright
2012-‐2013
BIOTEC
2
Country
Culture
Collec8ons
Cultures
Service
Collec8ons
Indonesia
18
11,476
5
Malaysia
7
2,915
2
Philippines
6
3,456
5
Singapore
3
1,389
0
Thailand
60
43,251
4
Viet
Nam
1
6,449
1
ASEAN
94
68,791
17
Sources:
h@p://www.wfcc.info/ccinfo/sta8s8cs/ updated
23
August
2013
Culture
Collec8ons
in
Thailand
and
ASEAN
3. 9/11/13 Copyright
2012-‐2013
BIOTEC
3
www.biotec.or.th/bcc/
Only BCC and
TISTR have their
own e-Catalog
and join the
GCM. (2 of 60)
www.Kstr.or.th/Kstr_culture/
4. 9/11/13 Copyright
2012-‐2013
BIOTEC
4
Culture
Collec8ons
#Cultures
registered
in
CCINFO
#Cultures
available
in
TNCC
BCC
(wdcm
783)
5,010
3,430
DMST
(wdcm
707)
10,000
442
DOA
(wdcm
704,
705)
76
1,163
TISTR
(wdcm
383)
2,425
515
Total
(TNCC)
17,511
5,550
UNIVERSITIES
25,740
TOTAL
43,251
Thailand Network on Culture
Collections (TNCC)
4 of 60 : Service Collections (7%)
http://www.biotec.or.th/tncc/
5. 9/11/13 Copyright
2012-‐2013
BIOTEC
5
http://www.biotec.or.th/tncc/
Culture
Collec8ons
#Cultures
registered
in
CCINFO
#Cultures
available
in
TNCC
BCC
(wdcm
783)
5,010
3,430
DMST
(wdcm
707)
10,000
442
DOA
(wdcm
704,
705)
76
1,163
TISTR
(wdcm
383)
2,425
515
Total
(TNCC)
17,511
5,550
UNIVERSITIES
25,740
TOTAL
43,251
Thailand Network on Culture
Collections (TNCC)
Thai Service Collections
6. TNCC
e-catalog
Direct Data Entry
l Easy to implement, but laborious data conversion,
data entry at data node
TNCC ( established since 2000)
Internal
DB
Data Conversion
File Transfer
=TNCC Data standard
= Data Update-
(Modify in-house DB,
Convert data, submit or
input the data)
Member
9/11/13 Copyright
2012-‐2013
BIOTEC
6
7. 9/11/13 Copyright
2012-‐2013
BIOTEC
7
Culture
Collec8ons
#Cultures
registered
in
CCINFO
#Cultures
available
in
TNCC
BCC
(wdcm
783)
5,010
3,430
DMST
(wdcm
707)
10,000
442
DOA
(wdcm
704,
705)
76
1,163
TISTR
(wdcm
383)
2,425
515
Total
(TNCC)
17,511
5,550
UNIVERSITIES
25,740
TOTAL
43,251
54 of 60 Research/
Specialized Collections
8. 9/11/13
Copyright
2012-‐2013
BIOTEC
8
TBRC,
Thailand
Bioresource
Research
Center
has
been
iniKated
by
BIOTEC,
to
• facilitate
the
integraKon
and
the
access
of
biomaterial
and
related
informaKon
beyond
the
scope
of
a
single
BRC,
and
•
fill
the
missing
link
between
BRC
and
industry
9. 9/11/13 Copyright
2012-‐2013
BIOTEC
9
TBRC
aims
to
(1)
leverage
the
network
strength
of
service
collecKons
(2)
create
a
network
of
research
collecKons
(3)
establish
a
common
technology
infrastructure
to
provide
standards
and
interoperable
informaKon
systems
across
the
network
levels
11. 9/11/13 Copyright
2012-‐2013
BIOTEC
11
TBRC member/hub
Management System
TBRC Central
Management System
TBRC Internet
Distribution System
TBRC-miner
TBRC-Link TBRC-catalog
Microbe++
Retrieve orders
Avail. Cultures
Mine for associated info
Categorization
Categorized microbe++
Submit orderDispatch order
TBRC-node
15. Tagged abstracts
Abstracts ”
from PubMed
PMID- 22782181
OWN – NLM
DP - 2012 Jul 11
TI - In vitro human cell-free expression system for
synthesis of malaria proteins.
AB - Malaria rhoptry genes (PFc14_0344,
PFc0120w, PY01759, PY00763, PY07482, and
PY04666) and a Maurer's cleft gene (PfA0680c)
identified from proteome analysis studies were cloned
into the pT7CFE1-Chis ...
AD - Department of Biological, Geological,
And Environmental Sciences,
PMID- 22782471
OWN – NLM
DP - 2012 Jul 11
TI - In vitro human cell-free expression system for
synthesis of malaria proteins.
AB - Malaria rhoptry genes (PFc14_0344, PFc0120w,
PY01759, in Plasmodium faciparum, ..
AD - Department of Biological, Geological,
And Environmental Sciences,
Enzyme Ontology
Pathway Ontology
KEGG pathways,
PANTHER pathways, …
Taxonomy
& gene info
from NCBI
PMID- 22782181
OWN – NLM
DP - 2012 Jul 11
TI - In vitro human cell-free expression system for
synthesis of malaria proteins.
AB - Malaria rhoptry genes (PFc14_0344,
PFc0120w, PY01759, PY00763, PY07482, and
PY04666) and a Maurer's cleft gene (PfA0680c)
identified from proteome analysis studies were cloned
into the pT7CFE1-Chis ...
AD - Department of Biological, Geological,
And Environmental Sciences,
PMID- 22782471
OWN – NLM
DP - 2012 Jul 11
TI - In vitro human cell-free expression system for
synthesis of malaria proteins.
AB - Malaria rhoptry genes (<START: GENE> PFc14_0344
<END>, <START:GENE> PFc0120w <END>
<START:GENE>PY01759 <END> , in <START: SPECIES>
Plasmodium faciparum <END>...
AD - Department of Biological, Geological,
And Environmental Sciences,
Microbe++ Search Engine
1
2
3
4
TBRC-‐miner:
Ontology-‐driven
Indexing
and
tagging
of
publicaKons
Enzyme
nomenclature
Industrial
ClassificaKon
9/11/13
Copyright
2012-‐2013
BIOTEC
15
16. 9/11/13 Copyright
2012-‐2013
BIOTEC
16
TBRC
:
Opening
2014
Become
the
informaKon
hub
for
microbial
resources
in
Thailand
Deliver
diverse
microbial
cultures
and
services
for
science
and
industry
www.tbrcnetwork.org