Task 2: Western blot trouble-shooting theory: Sadly, things don't always go as expected in the lab! Arg! A very real part of research is trying to figure out what might have gone wrong. Consider the examples below. a) Oh no! There are NO BANDS on the blot for tubulin. NONE! It looks like the one on the right. Your Ponceau stain indicated that there were cell lysate proteins on the nitrocellulose. Suggest one explanation that would generate this result:.