1. The study examined the role of CD24 in regulating tumor cell invasion through suppression of tissue factor pathway inhibitor-2 (TFPI-2) in a c-Src dependent manner. Knockdown of CD24 or overexpression of TFPI-2 significantly reduced invasion in lung, ovarian, and brain cancer cell lines.
2. Results demonstrated for the first time that CD24 regulates the expression of TFPI-2, a known tumor suppressor that inhibits invasiveness and metastasis. Depletion of CD24 reduced TFPI-2 expression and increased invasion.
3. The findings suggest that depletion of CD24 in malignant tumors may interfere with tumor progression by modulating TFPI-2 expression and cell
On March 14 I presented the history of my research activities and proposals for MS Biology thesis work for the students entering the program at National University,
Identification of Rare and Novel Alleles in FFPE Tumor Samples | ESHG 2015 Po...Thermo Fisher Scientific
Tumors are becoming recognized as genetically heterogeneous masses of cells with different clonal histories. Identifying the mutations present in these heterogeneous masses can lead to important insights into the future behavior of the tumor and possible intervention mechanisms. However, the rarity of pathogenic mutations in small subsets of cells can make identification of such alleles difficult. In this study, we demonstrate a complete workflow that facilitates the identification of rare and novel alleles from FFPE tumor sections. We collected small regions with different cellular morphologies from lung tumor samples using laser capture microdissection, extracted both DNA and RNA from these regions, and characterized mutations present and transcript abundances by using Ion AmpliSeq™ targeted sequencing. We show that LCM facilitates the detection of alleles that are not detectable in macrodissected tissue scrapes. We also show that different regions of a tumor have very different patterns of alleles detectable and have a great deal of genetic diversity. Finally, we show that RNA expression patterns are also clearly different in the different regions. Interestingly, dissected regions with similar gross tissue morphologies display differences in alleles present and RNA expression patterns. These results suggest how we may in the future use this method to analyze mutations present in a tumor is to microdissect different subregions of the tumor, and using Ion AmpliSeq™ panels to identify the alleles present in those subregions.
El 3 de noviembre de 2015, la Fundación Ramón Areces organizó en su sede en Madrid (C/ Vitruvio, 5) una jornada sobre ‘El cáncer como consecuencia del envejecimiento: posibles soluciones’. Coordinado por la investigadora María Vallet Regí, del Departamento de Química Inorgánica y Bioinorgánica de la Universidad Complutense de Madrid, contó con la presencia, entre otros científicos, de Mariano Barbacid, Lodovico Balducci y Theresa Guise.
On March 14 I presented the history of my research activities and proposals for MS Biology thesis work for the students entering the program at National University,
Identification of Rare and Novel Alleles in FFPE Tumor Samples | ESHG 2015 Po...Thermo Fisher Scientific
Tumors are becoming recognized as genetically heterogeneous masses of cells with different clonal histories. Identifying the mutations present in these heterogeneous masses can lead to important insights into the future behavior of the tumor and possible intervention mechanisms. However, the rarity of pathogenic mutations in small subsets of cells can make identification of such alleles difficult. In this study, we demonstrate a complete workflow that facilitates the identification of rare and novel alleles from FFPE tumor sections. We collected small regions with different cellular morphologies from lung tumor samples using laser capture microdissection, extracted both DNA and RNA from these regions, and characterized mutations present and transcript abundances by using Ion AmpliSeq™ targeted sequencing. We show that LCM facilitates the detection of alleles that are not detectable in macrodissected tissue scrapes. We also show that different regions of a tumor have very different patterns of alleles detectable and have a great deal of genetic diversity. Finally, we show that RNA expression patterns are also clearly different in the different regions. Interestingly, dissected regions with similar gross tissue morphologies display differences in alleles present and RNA expression patterns. These results suggest how we may in the future use this method to analyze mutations present in a tumor is to microdissect different subregions of the tumor, and using Ion AmpliSeq™ panels to identify the alleles present in those subregions.
El 3 de noviembre de 2015, la Fundación Ramón Areces organizó en su sede en Madrid (C/ Vitruvio, 5) una jornada sobre ‘El cáncer como consecuencia del envejecimiento: posibles soluciones’. Coordinado por la investigadora María Vallet Regí, del Departamento de Química Inorgánica y Bioinorgánica de la Universidad Complutense de Madrid, contó con la presencia, entre otros científicos, de Mariano Barbacid, Lodovico Balducci y Theresa Guise.
Stem Cells in A New Era of Cell based Therapies - Creative BiolabsCreative-Biolabs
A stem cell can replicate itself or differentiate into cells that carry out the specific functions of the body. The application of stem cells in regenerative medicine and disease therapeutics is one of the most exciting advances in medical science today. In cell-based therapies, stem cells may play two roles. The first role is as drug-delivery vehicles. The second role is as therapeutic agents themselves. Stem cells also offer opportunities for scientific advances that go far beyond cell-based therapies. Creative Biolabs is dedicated to facilitate the research of stem cells in both basic science and therapeutics development. Please contact us if you are interested in our services or products.
[PDF] Pressemitteilung: Neuer Bluttest identifiziert aggressive Tumoren mit Resistenzen gegen Strahlen- und Chemotherapien
[http://www.lifepr.de?boxid=371468]
Does your cell line have a secret? Avoid surprises with characterizationMerck Life Sciences
Watch the recording of this webinar here: https://bit.ly/2Y05bV4
The first step to avoiding an unpleasant and costly contamination event is characterization of your cell banks.
Regardless of the biotech product, careful characterization of the cell banks used in its production is the first step in mitigating the risk of a contamination event. In fact, cell line characterization is an important component of the overall viral safety strategy for the product. We will describe the testing necessary to ensure cell banks are free from infectious and other adverse agents and that meets current regulatory expectations. Different levels of testing are performed for master, working, and end of production cell banks, and the differences in testing for each of these types of banks will be discussed.
In this webinar, you will learn:
• The types of tests that are needed to fully characterize your cell banks
• The best tests to use for your particular cell line
• Reasons why a viral contaminant may be missed
Stem Cells in A New Era of Cell based Therapies - Creative BiolabsCreative-Biolabs
A stem cell can replicate itself or differentiate into cells that carry out the specific functions of the body. The application of stem cells in regenerative medicine and disease therapeutics is one of the most exciting advances in medical science today. In cell-based therapies, stem cells may play two roles. The first role is as drug-delivery vehicles. The second role is as therapeutic agents themselves. Stem cells also offer opportunities for scientific advances that go far beyond cell-based therapies. Creative Biolabs is dedicated to facilitate the research of stem cells in both basic science and therapeutics development. Please contact us if you are interested in our services or products.
[PDF] Pressemitteilung: Neuer Bluttest identifiziert aggressive Tumoren mit Resistenzen gegen Strahlen- und Chemotherapien
[http://www.lifepr.de?boxid=371468]
Does your cell line have a secret? Avoid surprises with characterizationMerck Life Sciences
Watch the recording of this webinar here: https://bit.ly/2Y05bV4
The first step to avoiding an unpleasant and costly contamination event is characterization of your cell banks.
Regardless of the biotech product, careful characterization of the cell banks used in its production is the first step in mitigating the risk of a contamination event. In fact, cell line characterization is an important component of the overall viral safety strategy for the product. We will describe the testing necessary to ensure cell banks are free from infectious and other adverse agents and that meets current regulatory expectations. Different levels of testing are performed for master, working, and end of production cell banks, and the differences in testing for each of these types of banks will be discussed.
In this webinar, you will learn:
• The types of tests that are needed to fully characterize your cell banks
• The best tests to use for your particular cell line
• Reasons why a viral contaminant may be missed
Now is the time to apply for the Asheville City Schools Board of Education. This document provides information about the position and the process for applying.
Asheville Bond Rating Increases to AAAGordon Smith
Standard & Poor's Ratings Services raised its rating on Asheville, N.C.'s general obligation (GO) debt one notch to 'AAA' from 'AA+'. The outlook is stable.
Esse Blog tem o intuito de interagir com educadores, que como eu, são eternos aprendizes. Hoje, Coordenadora Pedagógica do Programa Mais Educação/EM Clori, em busca de meios de ampliarmos nosso trabalho, com eficiência e eficácia, acredito que nesse blog teremos a oportunidade de interagirmos e nos aperfeiçoarmos!
Social Network Analysis en Semantic Web voor BibliothekenRory Sie
Presentatie voor de library school waarin kort uitgelegd wordt wat het semantic web en social network analysis zijn, en hoe ze belangrijk kunnen zijn voor bibliotheken
Objective: To probe into the influence of miR-21 on the proliferation as well as apoptosis of oral squamous cell carcinoma (OSCC) and its causative role.
Study Design: We adopted microarray for detecting the differentially expressed genes in OSCC tumor tis-sues and paracancerous tissues. We assessed the link of miR-21 expression with tumor size, lymph node metastasis, and tumor differentiation. We employed CCK-8 and EdU assay for detecting the impact of miR-21 inhibitor and miR-21 mimic on Cal-27 cell proliferation, as well as TUNEL and AnnexinV-FITC/PI double staining for detecting miR-21 expression on cell apoptosis. We forecasted the possible target of miR-21 via TargetScan, as well as detected the interaction of miR-21 with PTEN via luciferase reporter experiment. The function of miR-21 expression in PTEN signaling pathway was monitored via western blot. We constructed PTEN overexpression plasmid and conducted rescue experiment to evaluate overexpressed PTEN on miR-21–induced proliferation.
Results: Microarray and RT-qPCR indicated that miR-21 expression increased demonstrably in OSCC. Subsequently, statistical analysis showed that miR-21 expression was plainly correlated with tumor size, lymph node metastasis, tumor differentiation, and smoking history. CCK-8 and EdU method exhibited that miR-21 mimics manifestly promoted Cal-27 cell proliferation, while miR-21 inhibitor blatantly inhibited Cal-27 cell proliferation. TUNEL and V-FITC/PI double staining assay showed that miR-21 inhibitor conspicuously promoted Cal-27 cell apoptosis. CCK-8 and EdU assay exhibited that overexpressed PTEN abolished the pro-proliferation influence of miR-21 mimic. TUNEL and V-FITC/PI experiments pointed out that knocking down PTEN abrogated the pro-apoptosis impact of miR-21 inhibitor.
Conclusion: miR-21 contributes to OSCC cell proliferation via targeting PTEN and inhibits its apoptosis.
Keywords: Akt/PKB signaling pathway; apoptosis; biomarkers, tumor; carcinoma, squamous cell; cell line, tumor; cell proliferation; microRNAs; miR-21; miRNA-21; mouth neoplasms; oral cancer; oral squamous cell carcinoma; proliferation; real time PCR
In Vivo Bioluminescent / Fluorescent Imagingachang07
The presentation is an introduction to using bioluminescent "reagents" to evaluate drug efficacy in tumor models. This presentation briefly highlights one of many research platforms available at Caliper Life Sciences\' Discovery Alliances\' In Vivo Division that performs contract research for the life science community.
Hallmarks of cancer and radiopharmaceuticalsAlice Viana
In this presentation I review the article Hallmarks of cancer: next generation, from Hanahan and Weinberg, and make a parallel with potential and current targets of radiopharmaceuticals for diagnosis and treatment.
Enhanced NK cell adoptive antitumor effects against breast cancer in vitroRahul Gupta
This is the research paper which i have been choosen for presentation "Enhance NK cell adoptive antitumor effects against breast cancer in vitro via blockade of the Transforming Growth Factor-Beta".
As an uncommon malignant tumor, hypopharyngeal cancer accounts for 3–5% of head and neck tumors [1]. Most pathological types of hypopharyngeal cancer are squamous cell carcinoma. Due to the occult anatomical location of hypopharyngeal cancer and poor surgical effect, local recurrence or distant metastasis often occurs in patients with hypopharyngeal cancer following surgery.
Seminario final 2012 biología molecular.laura-sebastían
1. Laura María Másmela Gil.
Sebastián Gómez Restrepo.
School of Medicine.
3th Semester.
CD24 promotes tumor cell invasion by
suppressing tissue factor
pathway inhibitor-2 (TFPI-2) in a c-Src-
dependent fashion.
Niko Bretz , Aurelia Noske, Sascha Keller , Natalie Erbe-Hofmann,
Thomas Schlange , Alexei V. Salnikov ,Gerd Moldenhauer ,
Glen Kristiansen , Peter Altevogt.
2. Introduction.
CD24.
Importantly, CD24 is
often highly
Is a glycoprotein expressed on the expressed in human
cell surface of most B solid tumors (lung,
lymphocytes and mature brain,
granulocytes; Is a cell adhesion stomach, colorectal,
molecule. prostate, breast
and ovary) and is
associated with a
poor prognosis.
3. Introduction
Increase in
number of cells
composing a
tissue.
Tumour.
A benign tumor is a type
Malignant tumors are
of tumor does not
grow disproportionately cancerous. Cancer cells can
and aggressively, does not invade and damage tissues
invade surrounding tissues. and organs near the tumor
4. Introduction
TFPI-2 The first K domain inhibits
coagulation factor VIIa
complexed to tissue factor (TF).
Human TFPI is a
physiological inhibitor of
the extrinsic pathway of The second K domain inhibits
factor Xa.
coagulation and functions in
anticoagulation and anti-
inflammation. It is a
secreted protein with three The third K domain binds to
heparin.
Kunitz (K) domains.
5. Introduction
Tissue factor (TF) is a transmembrane
glycoprotein that initiates the extrinsic pathway of
coagulation cascade and acts as a
receptor and enzyme cofactor factor VII. Also is
directly related to the aggressive cancer.
6. Introduction
C Src.
Is an endogenous protein
from the family of
tyrosine kinases, which is
present in the cytosol of
the cell.
c-Src is the gene product
of the same proto-
oncogene c-Src, that is a
precursor of a potentially
cancer causing.
8. General Objective.
• Study the mechanism of cell
FPI-2
invasion with CD24-dependent CD24 knock-
down or transient over expression in human
cancer cell lines.
15. Materiales y métodos
Es una técnica que
permite medir
Utlización de FACS
simultáneamente
Citometría de flujo Canto II FlowJo
múltiples
software.
marcadores de una
sola célula.
16. Materiales y métodos
Western blot
Detección de proteínas
Lisis celular
Mediante una electroforesis en pesadas
gel: separación de proteínas
transferencia a membrana
absorbente
Detección: anticuerpo primario-
anticuerpo secundario
17. Materiales y métodos
Separación de
Muestra de la matriz
extracelular y de
células
para
detección de la
proteína TFPI-2 se
Sembrado celular
utilizo un
anticuerpo.
Análisis
de TFPI-2
18. Materiales y métodos
Procedimiento
histopatológic
Uso de
o de cánceres
anticuerpo
de mama
especifico, Localización
primarios,
marcado con Muestra del
Inmunohisto lesiones in situ
una enzima fijada en complejo
química. de carcinoma,
que puede parafina. antígeno-
metástasis
transformar anticuerpo.
recurrentes
CD24 en
locales y
visible.
distales.
19. Resultados
La caída de CD24 afecta la invasión de líneas
celulares cancerosas.
• Se demuestra el rol de CD24 en la regulación
de la invasión de células tumorales.
• La ausencia de CD24 reduce la
invasión de células tumorales humanas.
21. Resultados
En células de carcinoma colorrectal y de
páncreas CD24 fue expresado
para controlar la expresión génica
por un mecanismo desconocido hasta ahora.
Disminución de la
expresión del CD24.
El agotamiento trasitorio
de TFPI-2 modula
la invasión de células Aumenta el TPFI-2
cancerígenas en el En todas las líneas
pulmón celulares estudiadas.
23. Resultados
• Expresión del TFPI-2 inhibe la invasión
celular.
TFPI-2 juega un papel
importante en la regulación
de la invasión de células
tumorales.
24. Resultados
• Sobre- expresión de CD24, suprime el TFPI-2;
aumentando la invasión célular.
Isoformas.
CD24-Ala CD24-Val
La expresión
de ambas formas
reduce la
cantidad del TFPI-2.
25. Resultados
Agotamiento de CD24 afecta la actividad de C Src.
CD24 se localiza en las balsas de membrana junto
con el C Src.
El eje CD24-Src juega un papel importante en la
regulación y expresión del TFPI 2.
27. Discusión
AUTHOR WHAT HE/SHE SAID AGREE DESAGREE
konduri SD and “Importantly, the up-regulation of TFPI-2 mRNA X
others was not only detected in SKOV3ip but also in
HS683 and A549 cells. Concomitantly, in all
three cell lines we noticed a significantly
reduced cell invasion that was not seen in
SNB19 cells having no detectable levels of TFPI-
2.”
Sierko E, “Our findings establish a link between CD24 X
Wojtukiewicz and TFPI-2, a proteinase inhibitor known for its
MZ, Kisiel W important role in the inhibition of tumor cell
invasiveness, neoplastic growth, and
metastases formation”
28. DISCUSIÓN
AUTHOR WHAT HE/SHE SAID AGREE DESAGREE
Taniuchi K, “In the presence of CD24 the x
Nishimori I, cleavage of BART
Hollingsworth mRNA by G3BP was blocked
MA whereas both BART or
CD24 siRNA depletion resulted in
enhanced cell motility
and invasion”
Sierko E, “The TFPI-2 gene is considered a x
Wojtukiewicz tumor suppressor
MZ, Kisiel W gene as its down-regulation or loss
is associated with
increasing malignancy”
31. conclusiones
• 1. In summary the results demonstrate for the
first time that CD24 can regulate the
expression of TFPI-2.
• 2. it was showed that cellular invasion was
significantly reduced after CD24 knock down
or after TRPI-2 overexpression
32. conclusiones
• 3. these findings suggest that the depletion of
CD24 in malignant tumors might offer a new
possibility to interfere with tumor progression.
• 4. in clinical practice is important to
know about the expression of CD24 and TFPI-
2 in tumor invasion or no invasion of
many body cells for potential cancer
treatments.