This study aimed to determine the genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients. Samples were collected from 62 patients and genotyped using pulsed-field gel electrophoresis (PFGE). PFGE analysis found high genetic similarity between isolates from the same patient, with one patient showing identical strains across all three sample sites. Some strains from different patients were also genetically similar, suggesting possible transmission within the ICU. The study demonstrates PFGE is effective for analyzing intra- and inter-patient diversity of C. albicans strains.

1. Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients Seok-Mo Heo, Robert S. Sung, Frank A. Scannapieco and Elaine M. Haase Presented by: Susana Ríos Echavarría. Carolina Severiche Mena. Medicine- Third semester Universidad Pontificia Bolivariana.

2. INTRODUCTION

4. GENETIC vs PATHOLOGY

5. GENERAL OBJECTIVE Determine genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients.

7. 2. MUESTREO MICROBIANO DE LA CAVIDAD ORAL, TRÁQUEA Y PULMÓN. MATERIALES Y METODOS

8. 3. LISIS CELULAR EN GEL Y ADICIÓN DEL TAPÓN DE ENZIMA DE RESTRICCIÓN. MATERIALES Y METODOS

9. MATERIALES Y METODOS 4. PFGE (ELECTROFORESIS EN GEL DE CAMPO PULSADO) Y BANDAS DE ANÁLISIS Y PATRONES DE INTERPRETACIÓN.

15. DISCUSSION YES NOT Author’s Name What the author said? Is it related with the study? Adair CG, Gorman SP, Feron BM, et al. The endotracheal tube can serve as a reservoir for pathogens in VAP patients and can facilitate the transport of pathogens to the lungs. Shin JH, Park MR, Song JW, et al. The low rate of microevolution of C. albicans in ICU patients was attributed to insufficient time for strains to diversify.

16. DISCUSSION YES NOT Author’s Name What the author said? Is it related with the study? Voss A, Pfaller MA, Hollis Rj, et al. Comparison of the strains from different patients indicates that there may be a possibility of acquisition of the organism from environment after admission to the hospital. That’s why PFGE typing methods may be used to detect nosocomial transmission.

17. DISCUSSION YES NOT Author’s Name What the author said? Is it related with the study? Chen KW, Chen YC,Lo HJ, et al. MLST have been shown to be more discriminatory for C. albicans , but no other Candida species.

20. CAROLINA SEVERICHE MENA

21. SUSANA RIOS ECHAVARRIA