More Related Content
What's hot
What's hot (19)
Similar to Seminario Biología Molecular
Similar to Seminario Biología Molecular (20)
Recently uploaded
Recently uploaded (20)
Seminario Biología Molecular
- 1. Universidad Pontificia Bolivariana Facultad de ciencias de la salud Ana Sofía Córdoba III semestre
- 2. INTRODUCTION Symptoms: - Cough: Dry or produce thick - Difficulty breathing - High temperature - Loss of appetite - Sweating and shivering - Chest pain
- 3. INTRODUCTION Multiplex PCR RT PCR Multiplex RT PCR
- 4. GENERAL OBJECTIVE “Propose an accelerated method for determining the pathogen in a sample using multiplex solid-phase RT- PCR.”
- 5. MÉTODOS Multiplex RT PCR Cepas Multiplex RT-PCR es capaz de la detección simultánea de viroides (ARN) y eubacterias (ADN) Busca detectar simultáneamente un amplio espectro de agentes patógenos CEPAS DE BACTERIAS COLECCIONES DE CULTIVO Streptococcus pneumoniae ATCC 25923 Staphylococcus aureus ATCC 43300 Haemophilus influenzae ATCC 49247 Legionella pneumophila ATCC 33152 Pseudomonas aeruginosa ATCC 25668 - 10662 NCTC Klebsiella pneumoniae ATCC 13883 - 9633 NCTC Influenza A - SARS-Cov-2 -
- 7. RESULTADOS
- 8. RESULTADOS
- 9. DISCUSSION AUTHOR AUTHOR´S POSTULATES AGREE OR DISAGREE WITH THE ARTICLE Inglis P.W., Pappas M.C.R., Resende L.V., Grattapaglia Chiquito Almanza E., Acosta Gallegos J.A., García Álvarez N.C. They propose that is necessary to unify the method for isolating nucleic acids to create a universal method for detecting RNA and DNA pathogenic agents known as CTAB method Lung O., Fisher M., Beeston A., Hughes K.B., Clavijo A Scuderi G., Catara A.F., Licciardello G In most methods based on RT, the reaction is carried out in the liquid phase (without using biochips), and only the subsequent hybridization is performed on the chip Westin L., Xu X., Miller C., Wang L., Edman C.F., Nerenberg M Pemov A., Modi H., Chandler D.P., Bavykin S. Approaches have been proposed to carry out solid-phase enzymatic reactions to create compact, specialized biochips, developed for isothermal amplification, PCR bridge, and next-generation sequencing (NGS).
- 10. CONCLUSIONS The Multiplex RT PCR manages to identify among 8 of the most common causes which is responsible for presenting a clinical picture of pneumonia in a patient to start treatment promptly and correctly. Molecular biology is of the utmost importance for medicine, since thanks to discoveries and advances such as Multiplex RT PCR, it is possible to make an optimal and sooner diagnosis for the patient
- 11. MAPA CONCEPTUAL
- 12. ¡THANK YOU!