Eun Jeong Kim conducted an experiment to measure the production of oxygen from different weights of chicken liver exposed to hydrogen peroxide over time. Three weights of liver were tested: 3g, 9g, and 15g. The experiment found that more liver produced more oxygen and that production increased over time, with 15g producing the most. However, 9g and 15g produced similar amounts, possibly because the amount of hydrogen peroxide was not enough to break down fully with 15g of liver. The student identified areas for improvement, such as using a smaller flask to better collect oxygen.

1. Name: Eun Jeong Kim
Enzyme Experiment

3.
Enzyme Lab Report
Name: Eun Jeong Kim
Grade 10c
Aim
The purpose of the experiment is to find the speed of catalase reaction and amount of
oxygen when different weight of liver put in the Hydrogen Peroxide 1%.
Hypothesis
If chicken liver put Hydrogen Peroxide 1% in, It will produce some bubble and oxygen.
The oxygen can be collected in a gas collection cylinder and the gas collection cylinder
will show how much oxygen is produced. And if different weight of liver put Hydrogen
Peroxide in, the result will be different. If less chicken liver is put into Hydrogen Peroxide,
it will produce a small amount of oxygen and more chicken liver is put into Hydrogen
Peroxide, it will produce more oxygen. In this experiment, three different weights will be
used 3g, 9g and 15g. 3g of chicken liver will produce small amount of oxygen. 9g of
chicken liver will produce oxygen more than 3g of chicken liver. And15g of chicken liver
will produce oxygen more than 3g and 9g.
Variable
Input variable
Amount of Liver
‐Amount of liver 3g, 9g, 15g are going to be used in this experiment.
Output variable
Amount of oxygen produced
‐Amount of oxygen produced will measure using gas collection cylinder and a stopwatch
would be used to measure the time taken.
Control Variable
#1. Type of Liver
• Using chicken liver only in this experience would be kept exact result.
#2. Amount of Hydrogen Peroxide
• Using the same amount of Hydrogen Peroxide for each different grams of liver.

4. #3. Concentration of Hydrogen Peroxide 1%
• This would be kept constant by using the same concentration of Hydrogen
Peroxide 1% throughout the experiment.
#4. Time
• The production record at regular intervals of 30 seconds.
Materials
• 1 x Rubber tube
• 1 x Conical flask 250ml
• 1 x Stopwatch
• 3 x 3g Liver
• 3 x 9g Liver
• 3 x 15g Liver
• 1 x Electronic Balance
• 9 x 50 ml Hydrogen Peroxide 1%
• Dissecting Dish
• 1 x Cylinder 50 ml
• Gas collection cylinder
Method
1. Set up equipment as shown as in Diagram 1
2. Cut the liver three of 3g, 9g, 15g
3. Check the weight of the liver using electronic balance
4. Measure 50 ml of Hydrogen Peroxide using gas collection cylinder
5. Put 50 ml of Hydrogen Peroxide in to 250 ml conical flask
6. Put the cut liver into Hydrogen Peroxide in conical flask and put rubber tube on
the conical flask quickly
7. Start the stopwatch
8. Measure the amount of oxygen produced in gas collection cylinder in 30 seconds
intervals
9. Record date in 30 seconds intervals
10. Repeat method 2~9 two times
11. Repeat method 10 using different weight of liver

5. Data table
Table 1: Oxygen produced in gas collection cylinder in different weight of liver
Amount Oxygen produced in gas Time (Seconds)
of Liver (g) collection cylinder 30 60 90 120 150 180
Trial 1 6 8 9 11 14 15
Trial 2 4 6 7 9 11 13
3 Trial 3 6 10 14 16 20 24
Trial 1 10 18 27 40 54 67
Trial 2 11 17 24 34 43 53
9 Trial 3 9 16 25 36 45 60
Trial 1 10 19 29 40 54 67
Trial 2 10 18 28 39 48 56
15 Trial 3 10 15 25 35 45 55
Table 2: Average of oxygen produced in gas collection cylinder in different
weight of liver
Oxygen Produced in gas collection Cylinder
Amount of Time (Seconds)
Liver (g) 30 60 90 120 150 180
3 5.33 8.00 10.00 12.00 15.00 17.33
9 10.00 17.00 25.33 36.67 47.33 60.00
15 10.00 17.33 27.33 38.00 49.00 59.33

6. Diagram
Graph
Oxygen Prodced in Gas Collection
Cylinder in different weight of Liver
70
Amount of oxygen produced (cm3)
60
50
40
30 3
20 9
10 15
0
0 30 60 90 120 150 180
Time (s)

7. Conclusion
was very well. Oxygen in the gas collection cylinder was increased steadily in the course
of time. Bubble was also increased steadily. When one experiment finished, the Catalase
reaction still worked.
The result of this experiment, there is interesting pattern. The weight 3g, 9g and 15g of
liver was produced more oxygen at regular intervals. And they have pattern. The weight
3g of liver was increase to produce oxygen into the gas collection cylinder at regular
intervals 2 or 3 mm when we record the result at regular intervals 30 seconds. For
example, 3g liver produce oxygen that in 30 seconds, it was produced 5.33 mm of
oxygen. In 60 seconds, it was produced 8 mm of oxygen. And in 90 seconds, it was
produced 10 mm of oxygen. Finally, in 180 seconds, it produced 17.33 mm of oxygen.
The weight 15g of liver was increased to produce oxygen into gas collection cylinder at
regular intervals 10 mm when we record the result at regular intervals 30 seconds. The
oxygen was increased in a pattern. For example, in 60 seconds, It was produced 17.33
mm of oxygen. And in 90 seconds, it was produced 27.33 mm of oxygen. The oxygen
increased 10 mm. Next record was also increased 10 mm of oxygen. In 3g and 15g had a
pattern. However, the weight 9g of liver was different with 3g and 15g. The weight 9g of
liver also had a pattern. But the weight 9g of liver had two patterns. First one was that
the oxygen was increased regular intervals 7 mm. This pattern was appeared in the
opening part. However, latter, the oxygen was increased at regular intervals 10 mm. It
increase very quickly.
The oxygen and bubbles were produced when liver join to Hydrogen Peroxide in this
experiment. The Hydrogen Peroxide is broke down by Catalase. The Catalase is enzyme.
And this enzyme helps the Hydrogen Peroxide when Hydrogen Peroxide break down to
water and oxygen. And liver has Catalase. So when liver put into Hydrogen Peroxide,
Hydrogen Peroxide is broke down to water and oxygen by Catalase in liver. If the liver is
large, it can break down more Hydrogen Peroxide. So the weight 9g and 15g of liver
produced more oxygen than 3g of liver. However, 9g and 15g was not big different. This
is because the weight 15g of liver have more Catalase than 9g, but the amount of
Hydrogen Peroxide is same with 9g of liver. In the weight 9g of liver was enough the
Hydrogen Peroxide to break down to water and oxygen. But the weight 15g of liver was
overflow to break down to water and oxygen, It's unbalance. The Hydrogen Peroxide are
not enough for the weight 15g of liver break down Hydrogen Peroxide to water and
oxygen. So the result is similar with 9g and 15g of liver.
Evaluation
In this experiment, I identified some errors in materials. For example, we used bigger
conical flask than we need. We used 250 ml conical and we used 50 ml Hydrogen
Peroxide. The big conical flask was wasteful to collect oxygen. If we use 100 ml conical
flask, we could be collected more oxygen than we got in this experiment. Also, we used

8. two pieces of liver when we experience 9g livers. I think, this made error to collect
oxygen. If we used one piece of liver in 9g, the result will be small amount of oxygen
than 15g of liver. Next experiment, I will make an intensive about our experiment and I
will make good plan. I will try to make a success experiment.