Sanchita Dey is a microbiologist seeking a new career opportunity. She has over 2 years of experience in research and development conducting experiments involving fermentation, purification, identification and characterization of microbes. Her skills include microbial culturing techniques, molecular biology techniques like DNA extraction and PCR, and analytical equipment experience.

1. SANCHITA DEY Email: dsanchita427@gmail.com
Microbiologist ℡:+91-9986322167
SUMMARY
A hardworking,motivated individual with excellent communication skills, very organized with a
strong attention to details and time management. Energetic team player who takes prides in quality of
work.Looking for an opportunity to begin a new career while contributing to the success of the
business.
PROFESSIONAL EXPERIENCE
Research Microbiologist
Natural Capsules Limited- February 2019 to September 2019
Responsibilities:
• Performing process of Fermentation Operation 50L, 500LBioreactor.
• Preparation of seed media,production media, feed preparation, seedpreparation.
• Flask level experiment: Reuse of Antifoam, Reuse of Fermented Broth, and Reuse of
Substrate. Experiment with seed 2 to increaseconversion.
• Carryingoutregularbasicexperiments,tests-TLC,Microscopy,RedTest,Staining.
• Routine culturing, maintain culture, slant preparation, streaking,microscopy,performed
experiment on comparison of media.
• Performed Microbiological testing and analysis for finished product and raw materials.
• Fermentation: Planning and carrying out Trials and standardization. Performed Bioconversion
experiments.Recording,analyzing and interpreting data.
• Downstream process-Processing fermented trials and purification.
• Sterilefiltration.
• Documentation andSOP’s.
Research Trainee
ITC Life Sciences & Technology Centre- December 2017 to October 2018
Responsibilities:
• Purification and identification of 5000 culture, maintainingpure culture,stock preparation.
• Cultural media preparation and sterilization and growth promotion testing.
• Prepare samples for microbiological analysis using aseptic technique.
• Performed antibiotic sensitive test,motility tests,serial dilution,microbial limit
test,biochemical test,hemolysis test,antimicrobial test.
• Performd Gram staining,Microscopy,Identification and susceptibility testing of bacterial
isolates.
• Use of microbiology and molecular biology technique such as DNA extraction and PCR to
identify and characterize.
• His-tag Protein Purification using Ni-NTA Affinity column chromatography purification.
• Performed molecular technique-SDS PAGE,Electrophoresis, Sonication, Plasmid
purification,Protein induction,competent cell preparation.

2. SKILLS
• Microbial culturing techniques (including media preparation, spread plate, pour plate, streak
plate, SWAB).
• Basic techniques like media preparation, platting, microscopy, handling of
bacterial cultures and aseptic techniques.
• Practical knowledge of molecular biology techniques and instruments such as DNA
isolation, PCR, western blot analysis, protein extraction/purification, PAGE, SDS-PAGE.
• Electrophoresis technique (Gel electrophoresis, SDS-PAGE).
• Blotting techniques (mostly Western blotting, southern blotting).
• Skilled in performing aseptic techniques with experience in microbiology lab in
accordance with GLP, GMP and SOP.
• Practical knowledge in using Microscope, Electrophoresis apparatus, Centrifuge, Autoclave,
Vacuum filter, pH meter, Colony counter, PCR, Vortex mixer, Bioreactor, Water bath, Shaker
Incubator, Spectophotometer, Hot Over, deep freezer (-80C) Elisa reader, Electro Spinning
Unit, Sonicator, Anaerobic Chamber, Magnetic stirrer.
• Ability to maintain log book and interpret data statistically.
• Knowledge of analytical chemistry instruments such as HPLC, and Spectrophotometer.
• Knowledge and experience of Hemolysis test, Coagulase test for toxin producing bacteria
which cause foodborne illness.
• Experienced with DNA isolation, operation of Bioreactor, SDS-page, Blotting techniques,
PCR,Competent cell preparation and Protein purification.
Computational Skills
Operating Systems: Windows10/7
Software:Basics in computer, basics of MS-Word andMS-Excel
EDUCATION
M.Sc.in Microbiology from University Of Mysore, Karnataka with CGP-7.5 in 2017
ACADEMIC PROJECTS
• 3 Months project work on ‘Isolation, Characterization and Molecular Detection of E.coli
pathotypes’ at Defence Food Research Lab(DFRL)
TECHNICAL TRAININGS
• 6 months training in R&D department at Defense Food Research Lab(DFRL).
• 3 months training in Molecular Biology Department at Chromous Biotech.
• 6 months rigorous Internship in Microbiology department at GNRChospital, Guwahati.
DECLARATION
I, SANCHITA DEY, do hereby confirm that the above-furnished Information
given is true to the best of my knowledge.
Date:
Place: Bangalore. (SANCHITA DEY)