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Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
Resume
Innovative, self-starter, Pharmaceutical sciences Ph.D. with strong background in ocular molecular biology, biochemistry,
and drug discovery and development with specialization in pre-clinical development of novel in-vitro/in-vivo models of
disease. Expertise in development and execution of genetic, molecular, and ophthalmic research methods to support and
accelerate the drug discovery pipeline relating to hit-to-lead optimization of compounds. Enthusiastic about learning and
developing new scientific methodologies. Dynamic team player adept at providing leadership while also learning from
fellow team members. Experienced in managing multiple cross-functional projects simultaneously. Effective
communicator and teacher that can deliver complex scientific topics and ideas to audiences of varied expertise.
EDUCATION
The University of Arizona College of Pharmacy, Tucson AZ 2009 - 2014
• Ph.D. Pharmaceutical Sciences, with a focus in Drug Discovery and Development & Cancer Biology
Thesis: The Regulatory Significance and Molecular Targeting of novel non-B-DNA Secondary Structures Formed
from the PDGFR-Beta Core Promoter Nuclease Hypersensitivity Element
The University of Arizona, Tucson AZ 2002 - 2007
• BS Biochemistry and Molecular Biophysics
• BS Molecular and Cellular Biology
EXPERIENCE
National Institute of Environmental Health Sciences (NIEHS), RTP NC 2014 - present
Postdoctoral Research Fellow (Laboratory of Anton M. Jetten Ph.D.)
• Designed, executed, and coordinated, concurrent multi-institute scientific research and development projects
managing cross-disciplinary teams at Duke (NC), UNC (NC), National Eye Institute (MD), University of Arizona
(AZ), and National Institute of Environmental Health Sciences (NC). Management responsibilities included day-
to-day project progression, pitfalls, timeline, and quality control; deliverables included data analysis, manuscript
composition.
• Actively sought out and engaged in collaborations, with high profile thought leaders and high profile cross-
disciplinary scientists, that leveraged my expertise in molecular biology and biochemistry to overcome vexing
project goals, promote timely project progression, data acquisition/analysis, and manuscript composition.
• Contributing writer for NIEHS Environmental Factor, an internationally renowned monthly periodical.
• Developed, staffed, and directed, short-term pilot projects.
Pima Community College, Tucson AZ 2009 - 2014
Adjunct Faculty (Department of Science and Technology)
• Led instruction and presented complex scientific concepts at an easy to understand level in traditional lecture-
based, hybrid lecture/web-based, and self-paced web/discovery hybrid classes.
• Developed and maintained course websites in D2L and Adobe Dreamweaver to facilitate, discussion forums,
interactive study materials, online assessments including homework, quizzes, and exams.
• Implemented ‘Clicker cards’ an innovative and effective low/no-budget solution for lecture based classes to
survey knowledge and provide immediate feedback for class and instructor
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
• Instructor of Record for: Biotechnology & Instrumentation I & II; General Biology I, II; General Chemistry I, II,
& Introductory; Biology for Allied Health; Human Anatomy & Physiology I, II, & Introductory; Organic
Chemistry I & II
The University of Arizona College of Pharmacy, Tucson AZ 2009 - 2014
Graduate Student (Laboratory of Laurence H. Hurley Ph.D.)
• Awarded supplemental training funds for NIH R01 and wrote annual progress reports to granting agency to
maintain funding for 5-year term of the grant.
• Developed, validated, and implemented novel cell-based and cell-free assays for hit-to-lead drug discovery and
development with a team of medicinal chemists and structural biologists to identify structure activity
relationships, target ligand specificity, cytotoxicity, and efficacy threshold.
• Coordinated, trained, and managed teams of undergraduate scientists into highly functional compound screening
groups to facilitate high-throughput data acquisition.
• Elected student body president and appointed College of Pharmacy representative to nationally recognized
graduate and professional student council (GPSC) and helped raised over $300,000 to start University of Arizona
GPSC grant awards.
SKILLS
Molecular Biology & Biochemistry
DNA isolation RNA isolation Plasmid and genomic DNA isolation Flow Cytometry Fluorescent microscopy
reporter assays Molecular cloning Gateway molecular cloning Site-directed mutagenesis Genomic sequence analysis
Chromatin immunoprecipitation (ChIP) Chromatin immunoprecipitation sequencing (ChIP-Seq) Tandem affinity
Chromatin co-immunoprecipitation (Re-ChIP) Microarray sample prep and data analysis RNA-Seq sample prep and
analysis Protein expression in mammalian and bacterial systems Protein purification from mammalian and bacterial
systems Immunoprecipitation (IP) Co-Immunoprecipitation (CoIP) Tandem affinity immunoprecipitation for >95% pure
protein (Tap-IP) Tandem affinity co-immunoprecipitation (Tap-CoIP) Size exclusion chromatography Western blotting
Northern blotting Southern blotting South-western blotting Electromobility shift assay (EMSA) DNA footprinting
(radioactive and non-radioactive) Circular Dichroism (CD) Fluorescent resonance energy transfer (FRET) Molecular
beacon FRET Bioluminescence resonance energy transfer (BRET) Nano-BRET (luciferase based BRET)
Ophthalmic
Tonometry Optical coherence tomography (OCT) Electroretinography (ERG) In-vivo assessment of Aqueous humor
dynamics by gadolinium-enhanced MRI In-vivo anterior chamber micro-injection for viral delivery of transgenes to the
trabecular meshwork Laser-capture microdissection (LCM) of trabecular meshwork
Cell Culture
Cell line maintenance RNAi knockdowns Primary cell isolation and culture (from mouse trabecular meshwork)
Transfection methodologies (lipid-based, nucleofection, electroporation) Lentivirus and adenovirus infection Passive and
active generation of stable cell lines Cell cytotoxicity assays Cell Proliferation assays Cell migration assays Cell
viability assays
Animal Models
• Mouse and rat handling, oral gavage, intraperitoneal and tail injections,
Teaching
• Curriculum design and development, syllabus construction, lecturing
FELLOWSHIPS AND AWARDS
Intramural Research Trainee Award Fellowship 2014
National Institutes of Health/National Institute of Environmental Health Sciences
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
College of Pharmacy Deans Travel Award 2013
The University of Arizona College of Pharmacy
Excellence in Pharmaceutical Sciences Research Award 2012
The University of Arizona College of Pharmacy
Faculty Standards Teaching Award (Chemistry) 2011
Pima Community College
Faculty Standards Teaching Award (Biology) 2010
Pima Community College
Susan G. Komen Scholar-in-Training Award 2010
American Association of Cancer Research (AACR), 101st
Annual Meeting
Initiative for Minority Ph.D.’s Graduate Study Award 2009
National Institute of General Medical Sciences (GM062584)
PUBLICATIONS
1. Brown RV, Wang T, Chappeta R, Wu G, Onel B, Quijada H, Camp SM, Chiang ET, Garcia JGN, Gokhale V,
Yang D, Hurley LH Deekonda S, Gokhale V, Onel B, Yang D, and Hurley LH. A Unique G-quadruplex in the
PDGFR-β Core Promoter Nuclease Hypersensitive Element Can Be Selectively Targeted by an Ellipticine Analog
to Downregulate Gene Expression. (UNDER JACS REVIEW FOR 2017)
2. Brown RV, Onel B, Yang D, and Hurley LH. The importance of the PDGFR-β promoter based i-motif in
transcriptional regulation and identification of compounds that bind to this structure to effect transcription.
(UNDER JACS REVIEW FOR 2017)
3. Tao S, Tillotson J, Wijeratne EM, Xu YM, Kang M, Wu T, Lau EC, Mesa C, Mason DJ, Brown RV, La Clair JJ,
Gunatilaka AA, Zhang DD, Chapman E. Withaferin A Analogs That Target the AAA+ Chaperone p97. ACS
Chem Biol. 2015 Aug 21;10(8):1916-24
4. Frett B, Brown RV, Ma M, Hu W, Han H, and Li HY. Therapeutic Melting Pot of Never in Mitosis Gene A
Related Kinase 2 (Nek2): A Perspective on Nek2 as an Oncology Target and Recent Advancements in Nek2 Small
Molecule Inhibition. J Med Chem. 2014 Feb 25. [Epub ahead of print]
5. Brown RV, Gaerig VC, Simmons T, and Brooks TA. Helping Eve overcome ADAM: G-quadruplexes in the
ADAM-15 promoter as new molecular targets for breast cancer therapeutics. Molecules. 2013 Dec
5;18(12):15019-34.
6. Chen Y, Agrawal P, Brown RV, Hatzakis E, Hurley L, and Yang D. The major G-quadruplex formed in the
human platelet-derived growth factor receptor β promoter adopts a novel broken-strand structure in K+ solution.
J Am Chem Soc. 2012 Aug 15; 134(32):13220-3.
7. Brown RV, Danford FL, Gokhale V, Hurley LH, and Brooks TA. Demonstration that drug-targeted down-
regulation of MYC in non-Hodgkins lymphoma is directly mediated through the promoter G-quadruplex. J Biol
Chem. 2011 Nov 25;286(47):41018-27.
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
8. Brown RV and Hurley LH. DNA acting like RNA. Biochem Soc Trans. 2011 Apr;39(2):635-40.
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
Extended CV
The University of Arizona College of Pharmacy: Tucson, AZ 8/2009 – 10/2014
Ph.D. Pharmaceutical Sciences, with a focus in Drug Discovery and Development and Cancer Biology
Dissertation: The Regulatory Significance and Molecular Targeting of novel non-B-DNA Secondary
Structures Formed From the PDGFR-Beta Core Promoter Nuclease Hypersensitivity Element
The University of Arizona: Tucson, AZ 8/2002 – 6/2007
B.S. Biochemistry and Molecular Biophysics
B.S. Molecular and Cellular Biology
PROFESSIONAL EXPERIENCE
Postdoctoral Fellow 11/2014 – Present
National Institute of Health (NIH)
National Institute of Environmental Health Science (NIEHS)
Immune and Inflammatory Disease Laboratory
Advisor: Anton M. Jetten, Ph.D.
Graduate Student 8/2009 – 10/2014
The University of Arizona College of Pharmacy
Department of Pharmaceutical Sciences
Advisor: Laurence H. Hurley, Ph.D.
Adjunct Faculty 8/2009 – 10/2014
Pima Community College
Departments of Biology and Chemistry
Research Associate I 10/2008 - 8/2009
The University of Arizona College of Pharmacy
Department of Pharmacology and Toxicology
Supervisor: Tracy A. Brooks, Ph.D.
Research Technician I 5/2006 - 10/2008
The University of Arizona Medical Center
Department of Surgery
Supervisor: Ronald L. Heimark, Ph.D.
Laboratory Assistant 10/2003 - 5/2006
The University of Arizona College of Agriculture & Life Sciences
Department of Plant Sciences
Supervisor: Vicki Chandler, Ph.D.
Wildlife Express Engineer 5/2002 - 8/2003
Walt Disney World
Disney’s Animal Kingdom
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
PUBLICATIONS
9. Brown RV, Wang T, Chappeta R, Wu G, Onel B, Quijada H, Camp SM, Chiang ET, Garcia JGN, Gokhale V,
Yang D, Hurley LH Deekonda S, Gokhale V, Onel B, Yang D, and Hurley LH. A Unique G-quadruplex in the
PDGFR-β Core Promoter Nuclease Hypersensitive Element Can Be Selectively Targeted by an Ellipticine Analog
to Downregulate Gene Expression. (UNDER JACS REVIEW FOR 2017)
10. Brown RV, Onel B, Yang D, and Hurley LH. The importance of the PDGFR-β promoter based i-motif in
transcriptional regulation and identification of compounds that bind to this structure to effect transcription.
(UNDER JACS REVIEW FOR 2017)
11. Tao S, Tillotson J, Wijeratne EM, Xu YM, Kang M, Wu T, Lau EC, Mesa C, Mason DJ, Brown RV, La Clair JJ,
Gunatilaka AA, Zhang DD, Chapman E. Withaferin A Analogs That Target the AAA+ Chaperone p97. ACS
Chem Biol. 2015 Aug 21;10(8):1916-24
12. Frett B, Brown RV, Ma M, Hu W, Han H, and Li HY. Therapeutic Melting Pot of Never in Mitosis Gene A
Related Kinase 2 (Nek2): A Perspective on Nek2 as an Oncology Target and Recent Advancements in Nek2 Small
Molecule Inhibition. J Med Chem. 2014 Feb 25. [Epub ahead of print]
13. Brown RV, Gaerig VC, Simmons T, and Brooks TA. Helping Eve overcome ADAM: G-quadruplexes in the
ADAM-15 promoter as new molecular targets for breast cancer therapeutics. Molecules. 2013 Dec
5;18(12):15019-34.
14. Chen Y, Agrawal P, Brown RV, Hatzakis E, Hurley L, and Yang D. The major G-quadruplex formed in the
human platelet-derived growth factor receptor β promoter adopts a novel broken-strand structure in K+ solution.
J Am Chem Soc. 2012 Aug 15; 134(32):13220-3.
15. Brown RV, Danford FL, Gokhale V, Hurley LH, and Brooks TA. Demonstration that drug-targeted down-
regulation of MYC in non-Hodgkins lymphoma is directly mediated through the promoter G-quadruplex. J Biol
Chem. 2011 Nov 25;286(47):41018-27.
16. Brown RV and Hurley LH. DNA acting like RNA. Biochem Soc Trans. 2011 Apr;39(2):635-40.
ABSTRACTS
1. Brown RV, Foley J, Mahler BT, Wang Y, Borras T, Badea A, Johnson GA, Qian H, Kyathanahalli J, Kang HS,
Miller L, and Jetten AM. The transcriptional factor Glis1 regulates a critical subset of genes involved in the
pathogenesis of Primary Open Angle Glaucoma November 2016
2. Weil B, Brown RV, and Jetten AM. The transcriptional factor Glis3 plays a vital role in Primary Angle Closure
Glaucoma (PACG), NIH/NIEHS Summer Symposium. August 2016
3. Jeon K, Brown RV, Kang HS, and Jetten AJ. Glis3 regulates anterior-posterior patterning in hESC-derived
neural stem cells via the WNT signaling pathway, International Society for Stem Cell Research. June 2016
4. Bouldin J, Brown RV, Foley J, Yang XP, Kang HS, and Jetten AJ. Characterization of N- and C-terminal
transactivation domains in the transcriptional factor Glis1, NIH/NIEHS Summer Symposium. August 2015
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
5. Brown RV, Foley J, Yang XP, Kyathanahalli J, Kang HS, and Jetten AJ. Characterization of Glis1 mechanism of
action and physiological function, NIH/NIEHS Science Days Symposium. November 2015
6. Brown RV, Yang XP, Kang HS, and Jetten AJ. Characterization of Glis1 functional domains and mechanism of
action NIH/NIEHS Science Days Symposium. November 2014
7. Brown RV Onel B, Yang D, and Hurley LH. Both G-quadruplex and i-Motif forming sequences are important in
understanding mechanisms for transcriptional regulation of the PDGFR-β promoter. Quartets in G-major.
International Meeting on G-quadruplex Nucleic acids. July 2013.
8. Brown RV, Brooks TA, and Hurley LH. Elucidating biological roles of non-B-form DNA secondary structures
within the PDGFR-β core promoter. The American Chemical Society National Medicinal Chemistry Symposium.
June 2012.
9. Brown RV, Brooks TA, and Hurley LH. Direct demonstration of c-MYC G-quadruplex stabilization in vitro:
elucidating the CA46 system. Proceedings of the American Association of Cancer Research (AACR). April 2011.
10. Brown RV, Gaerig VC, Hurley LH, and Brooks TA. Helping Eve overcome ADAM: Characterization of the G-
quadruplex in the ADAM15 core promoter. Proceedings of the American Association of Cancer Research
(AACR), April 2010.
11. Gaerig VC, Fortin J, Brown RV, and Brooks TA. A novel approach to develop anti-VEGFR2/flk-1 therapeutics:
characterizing and targeting the G-quadruplex. Proceedings of the American Association of Cancer Research
(AACR). April 2010.
12. Brown RV, Gaerig VC, Hurley LH, and Brooks TA. Novel small molecule compounds targeting the c-MYC G-
quadruplex for the treatment of non-Hodgkin lymphoma. Graduate and Professional Student Showcase,
November 2009.
13. Brown RV, Gaerig VC, Hurley LH, and Brooks TA. Transcriptional down-regulation of c-Myc and Bcl-2 via
DNA secondary structure stabilization as novel therapy for non-Hodgkin lymphoma. AHSC Frontiers in
Biomedical Research, October 2009.
14. Gorgiev I, Shi J, Brown RV, and Heimark RL. Regulatory modules of N-Cadherin transcription of EMT of
epithelial cancers. October 2009.
15. Brown RV, Gaerig VC, Beman C, Pangasa M, Karolak MR, Hurley LH, and Brooks TA. Transcriptional down-
regulation of c-Myc and Bcl-2 via DNA secondary structure stabilization as novel therapy for non-Hodgkin
lymphoma. Proceedings of the American Association of Cancer Research (AACR). April 2009.
16. Brooks TA, Brown RV, Beman CS, Jenkins S, Flynn GA, Hurley LH, and Baker AF. Characterization of novel
DNA-interactive small molecule compounds targeting transcriptional regulation of c-Myc and Bcl-2 in pancreatic
cancer. Proceedings of the American Association of Cancer Research (AACR). April 2008.
17. Bair WB III, Brown RV, Gorgiev I, Zanello S, Heimark RL. Transcriptional repression of N-Cadherin in
differentiated prostate carcinomas. Cancer Biology Training Consortium. November 2007.
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
18. Brown RV, Viswesh VV, Sidorenko LV, Chandler VI. Alteration in DNA methylation of the endogenous b’ allele
upon exposure to the unstable factor for orange1 mutation. The University of Arizona Biochemistry and
Molecular Biophysics Research Symposium. April 2006.
INVITED & DEPARTMENTAL TALKS
1. Glis1 plays a vital role in the development and progression of ophthalmic disease, NIH/NIEHS, Immune,
Inflammation, and Disease Laboratories Work In Progress seminar series (2016)
2. Glis1 physiological function and mechanism of action, NIH/NIEHS, Immune, Inflammation, and Disease
Laboratories Work In Progress seminar series (2015)
3. Glis1 regulatory domains and transcriptional mechanism of action, NIH/NIEHS, Immune and Inflammation and
Disease Laboratories Work In Progress seminar series (2014)
4. Targeting novel non-B-DNA Secondary Structures Formed From the PDGFR-Beta Core Promoter University of
Arizona College of Pharmacy Drug Discovery and Development retreat, 30min talk (2013)
5. Both G-quadruplex and i-Motif DNA secondary structures are important in understanding PDGFR-β promoter
transcriptional regulation, University of Arizona College of Pharmacy Drug Discovery and Development retreat,
30min talk (2012)
6. A tale of two strands: Characterization and molecular targeting of the PDGFR-β promoter G-quadruplex and i-
Motif, University of Arizona College of Pharmacy Drug Discovery and Development retreat, 15min talk (2011)
7. Form and function of Non-B-form DNA secondary structures within the PDGFR-β core promoter, University of
Arizona College of Pharmacy Drug Discovery and Development retreat, 15min talk (2010)
8. Molecular targeting of the c-MYC G-quadruplex for the treatment of non-Hodgkin and Mantle cell lymphoma,
University of Arizona Leukemia and Lymphoma Society seminar series, 60min talk (2010)
9. Helping Eve overcome ADAM: Characterization and molecular targeting of the G-quadruplex in the ADAM15
core promoter, University of Arizona Comprehensive Cancer Center AACR seminar series, 15min talk ($500
travel award) (2009)
TEACHING EXPERIENCE
Traditional Lecture/Lab-Based Courses
BIO206: Biotechnology Instrumentation I 8/2012 - 12/2012
BIO207: Biotechnology Instrumentation II 1/2012 - 12/2013
CHM080: Preparation for General Chemistry 8/2011 - 12/2011
• Immediate feedback assessments (clicker & group discussion)
• Problem-based assessments (case-study based)
• Individual assessment (homework, quiz, exams)
• Traditional laboratory training
Hybrid Lecture & Internet/Lab-Based Course
BIO156: Introductory Biology for Allied Health 8/2009 - 5/2014
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
• Immediate feedback assessments (clicker & group discussion)
• Problem-based assessments (case-study based)
• Individual assessment (homework, quiz, exams)
• D2L and Blackboard assignments and discussions
• Traditional Laboratory training
Self-Paced Web/Discovery Lab Hybrid Courses
BIO100: Biology Concepts for Non-Majors 8/2009 - 5/2011
BIO105: Environmental Biology 8/2009 - 5/2011
BIO160: Introduction to Human Anatomy and Physiology 8/2009 - 5/2011
BIO181: General Biology I 8/2009 - 5/2011
BIO182: General Biology II 8/2009 - 5/2011
BIO183: Marine Biology 8/2009 - 5/2011
BIO201: Human Anatomy and Physiology I 8/2009 - 5/2011
BIO202: Human Anatomy and Physiology II 8/2009 - 5/2011
CHM235: General Organic Chemistry I 8/2009 - 5/2011
CHM236: General Organic Chemistry II 8/2009 - 5/2011
• Internet based course materials and lecture (D2L, Blackboard, Dreamweaver)
• Hybrid Web/Discovery-lab (discussion and participation)
• Individual assessment (homework, quiz, exams)
• Hybrid Web/Discovery-lab Laboratory training
HONORS AND AWARDS
Intramural Research Trainee Award Fellowship 2014
National Institutes of Health/National Institute of Environmental Health Sciences
College of Pharmacy Deans Travel Award 2013
The University of Arizona College of Pharmacy
Excellence in Pharmaceutical Sciences Research Award 2012
The University of Arizona College of Pharmacy
Faculty Standards Teaching Award (Chemistry) 2011
Pima Community College
Faculty Standards Teaching Award (Biology) 2010
Pima Community College
Susan G. Komen Scholar-in-Training Award 2010
American Association of Cancer Research (AACR), 101st
Annual Meeting
Initiative for Minority Ph.D.’s Graduate Study Award 2009
National Institute of General Medical Sciences (GM062584)
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
PROFESSIONAL CLUBS & SOCIETIES
Graduate and Professional Student Council 2012
The University of Arizona
Graduate Student Council 2010
The University of Arizona College of Pharmacy
American Chemical Society (ACS) 2010
American Chemical Society (ACS) Medicinal Chemistry Club 2010
The University of Arizona
American Association of Cancer Researchers (AACR) 2008
PROFESSIONAL LEADERSHIP POSITIONS
Reporter for Environmental Factor News Letter 2015- Present
NIEHS Office of Communication and Public Liaison
STaRS Lecturer (Topic: Biochemistry and Molecular Biology) 2015- 2016
NIEHS Office of Science Education & Diversity
Project Mentor Vernon Malone College and Career Academy 2015- 2016
NIEHS Office of Science Education & Diversity
Project Mentor Southern School of Energy and Sustainability 2015- 2016
NIEHS Office of Science Education & Diversity
Science Education Consultant for Vernon Malone College and Career Academy 2015- 2016
NIEHS Office of Science Education & Diversity
Science Education Consultant for Vernon Malone College and Career Academy 2015- 2016
NIEHS Office of Science Education & Diversity
DNA Day Ambassador 2012 - 2014
North Carolina DNA Day
College of Pharmacy Ph.D. Track Representative 2012 - 2014
The University of Arizona Graduate and Professional Student Council
President 2011 - 2014
The University of Arizona College of Pharmacy Graduate Student Council
Vice-President 2011 - 2012
The University of Arizona College of Pharmacy Graduate Student Council
Medicinal Chemistry Student Representative 2010 - 2011
The University of Arizona College of Pharmacy Graduate Student Council
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
President and Co-Founder 2009 - 2011
The University of Arizona American Chemical Society (ACS) Club
COMMITTEE INVOLVEMENT
NIH/NIEHS Committee
Immune, Inflammation and Disease Laboratory Annual Retreat Steering Committee
Role: Committee Member
Duties: Attend monthly meetings, participate in programmatic planning and decision-making for
departmental retreat. Serve on smaller event-specific focus committees organization and present findings
and recommendations to steering committee and committee chair
The University of Arizona College of Pharmacy Internal Committees
College of Pharmacy Graduate Student Council, Ph.D. Track
Role: Graduate Student Representative
Duties: Attend departmental meetings, act as a liaison between graduate students and faculty in the Ph.D.
track of the college of pharmacy, communicate the needs of graduate students to faculty, participate in
decision-making including but not limited to curriculum and career development on behalf of the graduate
students college of pharmacy, serve on appointed committees, assist in the organization of departmental
functions and solicit volunteers including but not limited to student recruitment, welcome incoming
graduate students and organize student orientation activities
College of Pharmacy Faculty Council,
Role: Graduate Student Representative
Duties: Attend College of Pharmacy faculty meetings, act as a liaison between graduate students and
faculty in the college of pharmacy, communicate the needs of graduate students to faculty, participate in
decision-making including but not limited to curriculum and career development on behalf of the graduate
students college of pharmacy and perform other responsibilities as required
College of Pharmacy Executive Council
Role: Graduate Student Representative
Duties: Attend College of Pharmacy executive council meetings, act as a liaison between graduate
students and faculty in the college of pharmacy, communicate the needs of graduate students to faculty,
participate in decision-making, serve on appointed committees, and perform other responsibilities as
required
College of Pharmacy Strategic Planning and Deep Dive Committee
Role: Graduate Student Representative
Duties: Assist in development and implementation of a 5 and 10 year strategic plan for growth and
development in the College of Pharmacy as a liaison between graduate students and faculty and
participate in decision-making
Scholarship and Awards Committee
Role: Graduate Student Representative
Duties: Assist in budget management and identification of new sources of scholarship funding for the
College of Pharmacy
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
Software and Technology Steering Committee
Role: Graduate Student Representative
Duties: Act as a liaison between graduate and IT committee to communicate the software needs of
graduate students in the College of Pharmacy
Medicinal Chemistry Student-Hosted Speaker Committee
Role: Committee Chair
Duties: Plan, organize, manage, and support the biannual identification and invitation of an invited
speaker for the College of Pharmacy medicinal chemistry track seminar series
The University of Arizona Graduate and Professional Student Council Internal Committees
Governing Documents Committee
Role: Committee Chair
Duties: Organize, manage, and support the University of Arizona graduate and professional student
council governing documents including but not limited to forming committees and recruiting volunteers
to review and assess suggested changes to the governing documents, to uphold the integrity of the policies
and to promote collaboration not litigation
Research and Project Grants Committee, Member
Role: Graduate Student Committee Member
Duties: Serve on a graduate student peer review panel to evaluate and score scientific applications
research project grants
Strategic Planning and Advisory Board, Co-Chair
Role: Graduate Student Committee Member
Duties: Assist in development and implementation of a 5 and 10 year strategic plan for growth and
development for the greater University of Arizona graduate and professional student council
Appropriations Committee, Member
Role: Graduate Student Committee Member
Duties: Serve on a graduate student panel to evaluate and ensure the proper management and allocation of
student fees paid to the graduate and professional student council
Scientific Review Committee, Member
Role: Graduate Student Committee Member
Duties: Serve on a graduate student peer review panel to evaluate and score scientific applications for
travel awards, professional opportunity and development awards
The University of Arizona Graduate and Professional Student Council External Committees
Faculty Senate
Role: Graduate Student Representative
Duties: Act as student liaison between the faculty at the University of Arizona and the graduate and
professional student council, communicate the needs of graduate students, participate in decision-making,
policy development, financial planning, and curricula development.
Research Policy Committee
Role: Graduate Student Representative
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
Duties: Act as a student liaison communicating matters relating to research, conflict of interest, data
retention, intellectual property, research ethics, research parks, interaction with industry, human and
animal research, and safety
Student Health Insurance Advisory Committee
Role: Graduate Student Advisor
Duties: Act as a student liaison communicating the needs of graduate students by negotiating insurance
policies and benefits, advocating for increased benefits, organizing educational seminars, for graduate
students and their families and assisting in website development and mass communication of insurance
benefits.
Student Services Fees Advisory Committee
Role: Graduate Student Representative
Duties: Act as a student liaison to evaluate communicate the needs of graduate students and ensure the
proper management and allocation of student fees paid to the University of Arizona for IT and Library
use
Diversity Coalition
Role: Graduate Student Representative
Duties: Act as minority student liaison to between minority graduate students and faculty to communicate
and assist in the strategic resolution of the needs and the challenges that face ethnic and demographic
minorities in the graduate colleges at the University of Arizona
MENTORING
Affiliated Programs
Biochemistry Program (BCP) 2011 - 2014
Role: Graduate Student Mentor
Undergraduate Biology Research Program (UBRP) 2011 - 2014
Role: Graduate Student Mentor
Initiative for Maximizing Student Diversity (IMSD) 2009 - 2014
Role: Graduate Student Mentor
Keeping Youth Engaged in Science (KEYS) 2009 - 2010
Role: Graduate Student Mentor
Students Trained
Brian Weil (Duke University) 2015 - 2016
Jordan Bouldin (UNC) 2014 - 2015
Megan A. Neeb (UBRP) 2012 - 2014
Carmen Lee (BCP) 2011 - 2014
Quinea R. Lassater (IMSD) 2011 - 2014
Shivani Phanse (BCP) 2011 - 2013
Alexa Willamson (UBRP) 2010 - 2011
Ravi Ram (UBRP) 2010 - 2011
Robert V. Brown, PhD
200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016
http://www.linkedin.com/RVBrownPhD
Forest L. Danford (UBRP) 2009 - 2011
Jessica Shelton (KEYS) 2009 - 2010
Jessica Kashien (IMSD) 2009 - 2010
Mathew R. Karolak (UBRP) 2009 - 2010
Misha Pangasa (BCP) 2008 - 2010
Vanessa C. Gaerig (BCP) 2008 - 2010
Lisette Rodriguez 2006 - 2008
Annan Kumar 2006 - 2008
Anthony J. Thomas 2006 - 2007
PROFESSIONAL & CHARACTER REFERENCES
Terete Borrás, Ph.D.
Affiliation: Collaborator, Character reference
Phone: (919) 843-0184
Email: tborras@med.unc.edu
Anton M. Jetten, Ph.D. (Please do not contact without prior consent from Robert V. Brown)
Affiliation: PI, Postdoctoral Advisor
Phone: (919) 541-2768
Email: jetten@niehs.nih.gov
W. Daniel Stamer, Ph.D.
Affiliation: Collaborator
Phone: (919) 681-1566
Email: william.stamer@dm.duke.edu
Caleb D. Sutherland, Ph.D.
Affiliation: Collaborator, Character reference
Phone: (919) 541-0189
Email: caleb.sutherland@nih.gov
Laurence H. Hurley, Ph.D.
Affiliation: PI, Predoctoral Ph.D. Advisor
Phone: (520) 626-5622
Email: hurley@pharmacy.arizona.edu
Tracy A. Brooks, Ph.D.
Affiliation: Collaborator, Character reference
Phone: (662) 915-2309
Email: tabrooks@olemiss.edu
Daekyu Sun, Ph.D.
Affiliation: Predoctoral Committee Member
Phone: (520) 626-0323
Email: sun@pharmacy.arizona.edu

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Resume_&_CV_RVB_12_15_16

  • 1. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD Resume Innovative, self-starter, Pharmaceutical sciences Ph.D. with strong background in ocular molecular biology, biochemistry, and drug discovery and development with specialization in pre-clinical development of novel in-vitro/in-vivo models of disease. Expertise in development and execution of genetic, molecular, and ophthalmic research methods to support and accelerate the drug discovery pipeline relating to hit-to-lead optimization of compounds. Enthusiastic about learning and developing new scientific methodologies. Dynamic team player adept at providing leadership while also learning from fellow team members. Experienced in managing multiple cross-functional projects simultaneously. Effective communicator and teacher that can deliver complex scientific topics and ideas to audiences of varied expertise. EDUCATION The University of Arizona College of Pharmacy, Tucson AZ 2009 - 2014 • Ph.D. Pharmaceutical Sciences, with a focus in Drug Discovery and Development & Cancer Biology Thesis: The Regulatory Significance and Molecular Targeting of novel non-B-DNA Secondary Structures Formed from the PDGFR-Beta Core Promoter Nuclease Hypersensitivity Element The University of Arizona, Tucson AZ 2002 - 2007 • BS Biochemistry and Molecular Biophysics • BS Molecular and Cellular Biology EXPERIENCE National Institute of Environmental Health Sciences (NIEHS), RTP NC 2014 - present Postdoctoral Research Fellow (Laboratory of Anton M. Jetten Ph.D.) • Designed, executed, and coordinated, concurrent multi-institute scientific research and development projects managing cross-disciplinary teams at Duke (NC), UNC (NC), National Eye Institute (MD), University of Arizona (AZ), and National Institute of Environmental Health Sciences (NC). Management responsibilities included day- to-day project progression, pitfalls, timeline, and quality control; deliverables included data analysis, manuscript composition. • Actively sought out and engaged in collaborations, with high profile thought leaders and high profile cross- disciplinary scientists, that leveraged my expertise in molecular biology and biochemistry to overcome vexing project goals, promote timely project progression, data acquisition/analysis, and manuscript composition. • Contributing writer for NIEHS Environmental Factor, an internationally renowned monthly periodical. • Developed, staffed, and directed, short-term pilot projects. Pima Community College, Tucson AZ 2009 - 2014 Adjunct Faculty (Department of Science and Technology) • Led instruction and presented complex scientific concepts at an easy to understand level in traditional lecture- based, hybrid lecture/web-based, and self-paced web/discovery hybrid classes. • Developed and maintained course websites in D2L and Adobe Dreamweaver to facilitate, discussion forums, interactive study materials, online assessments including homework, quizzes, and exams. • Implemented ‘Clicker cards’ an innovative and effective low/no-budget solution for lecture based classes to survey knowledge and provide immediate feedback for class and instructor
  • 2. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD • Instructor of Record for: Biotechnology & Instrumentation I & II; General Biology I, II; General Chemistry I, II, & Introductory; Biology for Allied Health; Human Anatomy & Physiology I, II, & Introductory; Organic Chemistry I & II The University of Arizona College of Pharmacy, Tucson AZ 2009 - 2014 Graduate Student (Laboratory of Laurence H. Hurley Ph.D.) • Awarded supplemental training funds for NIH R01 and wrote annual progress reports to granting agency to maintain funding for 5-year term of the grant. • Developed, validated, and implemented novel cell-based and cell-free assays for hit-to-lead drug discovery and development with a team of medicinal chemists and structural biologists to identify structure activity relationships, target ligand specificity, cytotoxicity, and efficacy threshold. • Coordinated, trained, and managed teams of undergraduate scientists into highly functional compound screening groups to facilitate high-throughput data acquisition. • Elected student body president and appointed College of Pharmacy representative to nationally recognized graduate and professional student council (GPSC) and helped raised over $300,000 to start University of Arizona GPSC grant awards. SKILLS Molecular Biology & Biochemistry DNA isolation RNA isolation Plasmid and genomic DNA isolation Flow Cytometry Fluorescent microscopy reporter assays Molecular cloning Gateway molecular cloning Site-directed mutagenesis Genomic sequence analysis Chromatin immunoprecipitation (ChIP) Chromatin immunoprecipitation sequencing (ChIP-Seq) Tandem affinity Chromatin co-immunoprecipitation (Re-ChIP) Microarray sample prep and data analysis RNA-Seq sample prep and analysis Protein expression in mammalian and bacterial systems Protein purification from mammalian and bacterial systems Immunoprecipitation (IP) Co-Immunoprecipitation (CoIP) Tandem affinity immunoprecipitation for >95% pure protein (Tap-IP) Tandem affinity co-immunoprecipitation (Tap-CoIP) Size exclusion chromatography Western blotting Northern blotting Southern blotting South-western blotting Electromobility shift assay (EMSA) DNA footprinting (radioactive and non-radioactive) Circular Dichroism (CD) Fluorescent resonance energy transfer (FRET) Molecular beacon FRET Bioluminescence resonance energy transfer (BRET) Nano-BRET (luciferase based BRET) Ophthalmic Tonometry Optical coherence tomography (OCT) Electroretinography (ERG) In-vivo assessment of Aqueous humor dynamics by gadolinium-enhanced MRI In-vivo anterior chamber micro-injection for viral delivery of transgenes to the trabecular meshwork Laser-capture microdissection (LCM) of trabecular meshwork Cell Culture Cell line maintenance RNAi knockdowns Primary cell isolation and culture (from mouse trabecular meshwork) Transfection methodologies (lipid-based, nucleofection, electroporation) Lentivirus and adenovirus infection Passive and active generation of stable cell lines Cell cytotoxicity assays Cell Proliferation assays Cell migration assays Cell viability assays Animal Models • Mouse and rat handling, oral gavage, intraperitoneal and tail injections, Teaching • Curriculum design and development, syllabus construction, lecturing FELLOWSHIPS AND AWARDS Intramural Research Trainee Award Fellowship 2014 National Institutes of Health/National Institute of Environmental Health Sciences
  • 3. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD College of Pharmacy Deans Travel Award 2013 The University of Arizona College of Pharmacy Excellence in Pharmaceutical Sciences Research Award 2012 The University of Arizona College of Pharmacy Faculty Standards Teaching Award (Chemistry) 2011 Pima Community College Faculty Standards Teaching Award (Biology) 2010 Pima Community College Susan G. Komen Scholar-in-Training Award 2010 American Association of Cancer Research (AACR), 101st Annual Meeting Initiative for Minority Ph.D.’s Graduate Study Award 2009 National Institute of General Medical Sciences (GM062584) PUBLICATIONS 1. Brown RV, Wang T, Chappeta R, Wu G, Onel B, Quijada H, Camp SM, Chiang ET, Garcia JGN, Gokhale V, Yang D, Hurley LH Deekonda S, Gokhale V, Onel B, Yang D, and Hurley LH. A Unique G-quadruplex in the PDGFR-β Core Promoter Nuclease Hypersensitive Element Can Be Selectively Targeted by an Ellipticine Analog to Downregulate Gene Expression. (UNDER JACS REVIEW FOR 2017) 2. Brown RV, Onel B, Yang D, and Hurley LH. The importance of the PDGFR-β promoter based i-motif in transcriptional regulation and identification of compounds that bind to this structure to effect transcription. (UNDER JACS REVIEW FOR 2017) 3. Tao S, Tillotson J, Wijeratne EM, Xu YM, Kang M, Wu T, Lau EC, Mesa C, Mason DJ, Brown RV, La Clair JJ, Gunatilaka AA, Zhang DD, Chapman E. Withaferin A Analogs That Target the AAA+ Chaperone p97. ACS Chem Biol. 2015 Aug 21;10(8):1916-24 4. Frett B, Brown RV, Ma M, Hu W, Han H, and Li HY. Therapeutic Melting Pot of Never in Mitosis Gene A Related Kinase 2 (Nek2): A Perspective on Nek2 as an Oncology Target and Recent Advancements in Nek2 Small Molecule Inhibition. J Med Chem. 2014 Feb 25. [Epub ahead of print] 5. Brown RV, Gaerig VC, Simmons T, and Brooks TA. Helping Eve overcome ADAM: G-quadruplexes in the ADAM-15 promoter as new molecular targets for breast cancer therapeutics. Molecules. 2013 Dec 5;18(12):15019-34. 6. Chen Y, Agrawal P, Brown RV, Hatzakis E, Hurley L, and Yang D. The major G-quadruplex formed in the human platelet-derived growth factor receptor β promoter adopts a novel broken-strand structure in K+ solution. J Am Chem Soc. 2012 Aug 15; 134(32):13220-3. 7. Brown RV, Danford FL, Gokhale V, Hurley LH, and Brooks TA. Demonstration that drug-targeted down- regulation of MYC in non-Hodgkins lymphoma is directly mediated through the promoter G-quadruplex. J Biol Chem. 2011 Nov 25;286(47):41018-27.
  • 4. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD 8. Brown RV and Hurley LH. DNA acting like RNA. Biochem Soc Trans. 2011 Apr;39(2):635-40.
  • 5. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD Extended CV The University of Arizona College of Pharmacy: Tucson, AZ 8/2009 – 10/2014 Ph.D. Pharmaceutical Sciences, with a focus in Drug Discovery and Development and Cancer Biology Dissertation: The Regulatory Significance and Molecular Targeting of novel non-B-DNA Secondary Structures Formed From the PDGFR-Beta Core Promoter Nuclease Hypersensitivity Element The University of Arizona: Tucson, AZ 8/2002 – 6/2007 B.S. Biochemistry and Molecular Biophysics B.S. Molecular and Cellular Biology PROFESSIONAL EXPERIENCE Postdoctoral Fellow 11/2014 – Present National Institute of Health (NIH) National Institute of Environmental Health Science (NIEHS) Immune and Inflammatory Disease Laboratory Advisor: Anton M. Jetten, Ph.D. Graduate Student 8/2009 – 10/2014 The University of Arizona College of Pharmacy Department of Pharmaceutical Sciences Advisor: Laurence H. Hurley, Ph.D. Adjunct Faculty 8/2009 – 10/2014 Pima Community College Departments of Biology and Chemistry Research Associate I 10/2008 - 8/2009 The University of Arizona College of Pharmacy Department of Pharmacology and Toxicology Supervisor: Tracy A. Brooks, Ph.D. Research Technician I 5/2006 - 10/2008 The University of Arizona Medical Center Department of Surgery Supervisor: Ronald L. Heimark, Ph.D. Laboratory Assistant 10/2003 - 5/2006 The University of Arizona College of Agriculture & Life Sciences Department of Plant Sciences Supervisor: Vicki Chandler, Ph.D. Wildlife Express Engineer 5/2002 - 8/2003 Walt Disney World Disney’s Animal Kingdom
  • 6. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD PUBLICATIONS 9. Brown RV, Wang T, Chappeta R, Wu G, Onel B, Quijada H, Camp SM, Chiang ET, Garcia JGN, Gokhale V, Yang D, Hurley LH Deekonda S, Gokhale V, Onel B, Yang D, and Hurley LH. A Unique G-quadruplex in the PDGFR-β Core Promoter Nuclease Hypersensitive Element Can Be Selectively Targeted by an Ellipticine Analog to Downregulate Gene Expression. (UNDER JACS REVIEW FOR 2017) 10. Brown RV, Onel B, Yang D, and Hurley LH. The importance of the PDGFR-β promoter based i-motif in transcriptional regulation and identification of compounds that bind to this structure to effect transcription. (UNDER JACS REVIEW FOR 2017) 11. Tao S, Tillotson J, Wijeratne EM, Xu YM, Kang M, Wu T, Lau EC, Mesa C, Mason DJ, Brown RV, La Clair JJ, Gunatilaka AA, Zhang DD, Chapman E. Withaferin A Analogs That Target the AAA+ Chaperone p97. ACS Chem Biol. 2015 Aug 21;10(8):1916-24 12. Frett B, Brown RV, Ma M, Hu W, Han H, and Li HY. Therapeutic Melting Pot of Never in Mitosis Gene A Related Kinase 2 (Nek2): A Perspective on Nek2 as an Oncology Target and Recent Advancements in Nek2 Small Molecule Inhibition. J Med Chem. 2014 Feb 25. [Epub ahead of print] 13. Brown RV, Gaerig VC, Simmons T, and Brooks TA. Helping Eve overcome ADAM: G-quadruplexes in the ADAM-15 promoter as new molecular targets for breast cancer therapeutics. Molecules. 2013 Dec 5;18(12):15019-34. 14. Chen Y, Agrawal P, Brown RV, Hatzakis E, Hurley L, and Yang D. The major G-quadruplex formed in the human platelet-derived growth factor receptor β promoter adopts a novel broken-strand structure in K+ solution. J Am Chem Soc. 2012 Aug 15; 134(32):13220-3. 15. Brown RV, Danford FL, Gokhale V, Hurley LH, and Brooks TA. Demonstration that drug-targeted down- regulation of MYC in non-Hodgkins lymphoma is directly mediated through the promoter G-quadruplex. J Biol Chem. 2011 Nov 25;286(47):41018-27. 16. Brown RV and Hurley LH. DNA acting like RNA. Biochem Soc Trans. 2011 Apr;39(2):635-40. ABSTRACTS 1. Brown RV, Foley J, Mahler BT, Wang Y, Borras T, Badea A, Johnson GA, Qian H, Kyathanahalli J, Kang HS, Miller L, and Jetten AM. The transcriptional factor Glis1 regulates a critical subset of genes involved in the pathogenesis of Primary Open Angle Glaucoma November 2016 2. Weil B, Brown RV, and Jetten AM. The transcriptional factor Glis3 plays a vital role in Primary Angle Closure Glaucoma (PACG), NIH/NIEHS Summer Symposium. August 2016 3. Jeon K, Brown RV, Kang HS, and Jetten AJ. Glis3 regulates anterior-posterior patterning in hESC-derived neural stem cells via the WNT signaling pathway, International Society for Stem Cell Research. June 2016 4. Bouldin J, Brown RV, Foley J, Yang XP, Kang HS, and Jetten AJ. Characterization of N- and C-terminal transactivation domains in the transcriptional factor Glis1, NIH/NIEHS Summer Symposium. August 2015
  • 7. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD 5. Brown RV, Foley J, Yang XP, Kyathanahalli J, Kang HS, and Jetten AJ. Characterization of Glis1 mechanism of action and physiological function, NIH/NIEHS Science Days Symposium. November 2015 6. Brown RV, Yang XP, Kang HS, and Jetten AJ. Characterization of Glis1 functional domains and mechanism of action NIH/NIEHS Science Days Symposium. November 2014 7. Brown RV Onel B, Yang D, and Hurley LH. Both G-quadruplex and i-Motif forming sequences are important in understanding mechanisms for transcriptional regulation of the PDGFR-β promoter. Quartets in G-major. International Meeting on G-quadruplex Nucleic acids. July 2013. 8. Brown RV, Brooks TA, and Hurley LH. Elucidating biological roles of non-B-form DNA secondary structures within the PDGFR-β core promoter. The American Chemical Society National Medicinal Chemistry Symposium. June 2012. 9. Brown RV, Brooks TA, and Hurley LH. Direct demonstration of c-MYC G-quadruplex stabilization in vitro: elucidating the CA46 system. Proceedings of the American Association of Cancer Research (AACR). April 2011. 10. Brown RV, Gaerig VC, Hurley LH, and Brooks TA. Helping Eve overcome ADAM: Characterization of the G- quadruplex in the ADAM15 core promoter. Proceedings of the American Association of Cancer Research (AACR), April 2010. 11. Gaerig VC, Fortin J, Brown RV, and Brooks TA. A novel approach to develop anti-VEGFR2/flk-1 therapeutics: characterizing and targeting the G-quadruplex. Proceedings of the American Association of Cancer Research (AACR). April 2010. 12. Brown RV, Gaerig VC, Hurley LH, and Brooks TA. Novel small molecule compounds targeting the c-MYC G- quadruplex for the treatment of non-Hodgkin lymphoma. Graduate and Professional Student Showcase, November 2009. 13. Brown RV, Gaerig VC, Hurley LH, and Brooks TA. Transcriptional down-regulation of c-Myc and Bcl-2 via DNA secondary structure stabilization as novel therapy for non-Hodgkin lymphoma. AHSC Frontiers in Biomedical Research, October 2009. 14. Gorgiev I, Shi J, Brown RV, and Heimark RL. Regulatory modules of N-Cadherin transcription of EMT of epithelial cancers. October 2009. 15. Brown RV, Gaerig VC, Beman C, Pangasa M, Karolak MR, Hurley LH, and Brooks TA. Transcriptional down- regulation of c-Myc and Bcl-2 via DNA secondary structure stabilization as novel therapy for non-Hodgkin lymphoma. Proceedings of the American Association of Cancer Research (AACR). April 2009. 16. Brooks TA, Brown RV, Beman CS, Jenkins S, Flynn GA, Hurley LH, and Baker AF. Characterization of novel DNA-interactive small molecule compounds targeting transcriptional regulation of c-Myc and Bcl-2 in pancreatic cancer. Proceedings of the American Association of Cancer Research (AACR). April 2008. 17. Bair WB III, Brown RV, Gorgiev I, Zanello S, Heimark RL. Transcriptional repression of N-Cadherin in differentiated prostate carcinomas. Cancer Biology Training Consortium. November 2007.
  • 8. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD 18. Brown RV, Viswesh VV, Sidorenko LV, Chandler VI. Alteration in DNA methylation of the endogenous b’ allele upon exposure to the unstable factor for orange1 mutation. The University of Arizona Biochemistry and Molecular Biophysics Research Symposium. April 2006. INVITED & DEPARTMENTAL TALKS 1. Glis1 plays a vital role in the development and progression of ophthalmic disease, NIH/NIEHS, Immune, Inflammation, and Disease Laboratories Work In Progress seminar series (2016) 2. Glis1 physiological function and mechanism of action, NIH/NIEHS, Immune, Inflammation, and Disease Laboratories Work In Progress seminar series (2015) 3. Glis1 regulatory domains and transcriptional mechanism of action, NIH/NIEHS, Immune and Inflammation and Disease Laboratories Work In Progress seminar series (2014) 4. Targeting novel non-B-DNA Secondary Structures Formed From the PDGFR-Beta Core Promoter University of Arizona College of Pharmacy Drug Discovery and Development retreat, 30min talk (2013) 5. Both G-quadruplex and i-Motif DNA secondary structures are important in understanding PDGFR-β promoter transcriptional regulation, University of Arizona College of Pharmacy Drug Discovery and Development retreat, 30min talk (2012) 6. A tale of two strands: Characterization and molecular targeting of the PDGFR-β promoter G-quadruplex and i- Motif, University of Arizona College of Pharmacy Drug Discovery and Development retreat, 15min talk (2011) 7. Form and function of Non-B-form DNA secondary structures within the PDGFR-β core promoter, University of Arizona College of Pharmacy Drug Discovery and Development retreat, 15min talk (2010) 8. Molecular targeting of the c-MYC G-quadruplex for the treatment of non-Hodgkin and Mantle cell lymphoma, University of Arizona Leukemia and Lymphoma Society seminar series, 60min talk (2010) 9. Helping Eve overcome ADAM: Characterization and molecular targeting of the G-quadruplex in the ADAM15 core promoter, University of Arizona Comprehensive Cancer Center AACR seminar series, 15min talk ($500 travel award) (2009) TEACHING EXPERIENCE Traditional Lecture/Lab-Based Courses BIO206: Biotechnology Instrumentation I 8/2012 - 12/2012 BIO207: Biotechnology Instrumentation II 1/2012 - 12/2013 CHM080: Preparation for General Chemistry 8/2011 - 12/2011 • Immediate feedback assessments (clicker & group discussion) • Problem-based assessments (case-study based) • Individual assessment (homework, quiz, exams) • Traditional laboratory training Hybrid Lecture & Internet/Lab-Based Course BIO156: Introductory Biology for Allied Health 8/2009 - 5/2014
  • 9. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD • Immediate feedback assessments (clicker & group discussion) • Problem-based assessments (case-study based) • Individual assessment (homework, quiz, exams) • D2L and Blackboard assignments and discussions • Traditional Laboratory training Self-Paced Web/Discovery Lab Hybrid Courses BIO100: Biology Concepts for Non-Majors 8/2009 - 5/2011 BIO105: Environmental Biology 8/2009 - 5/2011 BIO160: Introduction to Human Anatomy and Physiology 8/2009 - 5/2011 BIO181: General Biology I 8/2009 - 5/2011 BIO182: General Biology II 8/2009 - 5/2011 BIO183: Marine Biology 8/2009 - 5/2011 BIO201: Human Anatomy and Physiology I 8/2009 - 5/2011 BIO202: Human Anatomy and Physiology II 8/2009 - 5/2011 CHM235: General Organic Chemistry I 8/2009 - 5/2011 CHM236: General Organic Chemistry II 8/2009 - 5/2011 • Internet based course materials and lecture (D2L, Blackboard, Dreamweaver) • Hybrid Web/Discovery-lab (discussion and participation) • Individual assessment (homework, quiz, exams) • Hybrid Web/Discovery-lab Laboratory training HONORS AND AWARDS Intramural Research Trainee Award Fellowship 2014 National Institutes of Health/National Institute of Environmental Health Sciences College of Pharmacy Deans Travel Award 2013 The University of Arizona College of Pharmacy Excellence in Pharmaceutical Sciences Research Award 2012 The University of Arizona College of Pharmacy Faculty Standards Teaching Award (Chemistry) 2011 Pima Community College Faculty Standards Teaching Award (Biology) 2010 Pima Community College Susan G. Komen Scholar-in-Training Award 2010 American Association of Cancer Research (AACR), 101st Annual Meeting Initiative for Minority Ph.D.’s Graduate Study Award 2009 National Institute of General Medical Sciences (GM062584)
  • 10. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD PROFESSIONAL CLUBS & SOCIETIES Graduate and Professional Student Council 2012 The University of Arizona Graduate Student Council 2010 The University of Arizona College of Pharmacy American Chemical Society (ACS) 2010 American Chemical Society (ACS) Medicinal Chemistry Club 2010 The University of Arizona American Association of Cancer Researchers (AACR) 2008 PROFESSIONAL LEADERSHIP POSITIONS Reporter for Environmental Factor News Letter 2015- Present NIEHS Office of Communication and Public Liaison STaRS Lecturer (Topic: Biochemistry and Molecular Biology) 2015- 2016 NIEHS Office of Science Education & Diversity Project Mentor Vernon Malone College and Career Academy 2015- 2016 NIEHS Office of Science Education & Diversity Project Mentor Southern School of Energy and Sustainability 2015- 2016 NIEHS Office of Science Education & Diversity Science Education Consultant for Vernon Malone College and Career Academy 2015- 2016 NIEHS Office of Science Education & Diversity Science Education Consultant for Vernon Malone College and Career Academy 2015- 2016 NIEHS Office of Science Education & Diversity DNA Day Ambassador 2012 - 2014 North Carolina DNA Day College of Pharmacy Ph.D. Track Representative 2012 - 2014 The University of Arizona Graduate and Professional Student Council President 2011 - 2014 The University of Arizona College of Pharmacy Graduate Student Council Vice-President 2011 - 2012 The University of Arizona College of Pharmacy Graduate Student Council Medicinal Chemistry Student Representative 2010 - 2011 The University of Arizona College of Pharmacy Graduate Student Council
  • 11. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD President and Co-Founder 2009 - 2011 The University of Arizona American Chemical Society (ACS) Club COMMITTEE INVOLVEMENT NIH/NIEHS Committee Immune, Inflammation and Disease Laboratory Annual Retreat Steering Committee Role: Committee Member Duties: Attend monthly meetings, participate in programmatic planning and decision-making for departmental retreat. Serve on smaller event-specific focus committees organization and present findings and recommendations to steering committee and committee chair The University of Arizona College of Pharmacy Internal Committees College of Pharmacy Graduate Student Council, Ph.D. Track Role: Graduate Student Representative Duties: Attend departmental meetings, act as a liaison between graduate students and faculty in the Ph.D. track of the college of pharmacy, communicate the needs of graduate students to faculty, participate in decision-making including but not limited to curriculum and career development on behalf of the graduate students college of pharmacy, serve on appointed committees, assist in the organization of departmental functions and solicit volunteers including but not limited to student recruitment, welcome incoming graduate students and organize student orientation activities College of Pharmacy Faculty Council, Role: Graduate Student Representative Duties: Attend College of Pharmacy faculty meetings, act as a liaison between graduate students and faculty in the college of pharmacy, communicate the needs of graduate students to faculty, participate in decision-making including but not limited to curriculum and career development on behalf of the graduate students college of pharmacy and perform other responsibilities as required College of Pharmacy Executive Council Role: Graduate Student Representative Duties: Attend College of Pharmacy executive council meetings, act as a liaison between graduate students and faculty in the college of pharmacy, communicate the needs of graduate students to faculty, participate in decision-making, serve on appointed committees, and perform other responsibilities as required College of Pharmacy Strategic Planning and Deep Dive Committee Role: Graduate Student Representative Duties: Assist in development and implementation of a 5 and 10 year strategic plan for growth and development in the College of Pharmacy as a liaison between graduate students and faculty and participate in decision-making Scholarship and Awards Committee Role: Graduate Student Representative Duties: Assist in budget management and identification of new sources of scholarship funding for the College of Pharmacy
  • 12. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD Software and Technology Steering Committee Role: Graduate Student Representative Duties: Act as a liaison between graduate and IT committee to communicate the software needs of graduate students in the College of Pharmacy Medicinal Chemistry Student-Hosted Speaker Committee Role: Committee Chair Duties: Plan, organize, manage, and support the biannual identification and invitation of an invited speaker for the College of Pharmacy medicinal chemistry track seminar series The University of Arizona Graduate and Professional Student Council Internal Committees Governing Documents Committee Role: Committee Chair Duties: Organize, manage, and support the University of Arizona graduate and professional student council governing documents including but not limited to forming committees and recruiting volunteers to review and assess suggested changes to the governing documents, to uphold the integrity of the policies and to promote collaboration not litigation Research and Project Grants Committee, Member Role: Graduate Student Committee Member Duties: Serve on a graduate student peer review panel to evaluate and score scientific applications research project grants Strategic Planning and Advisory Board, Co-Chair Role: Graduate Student Committee Member Duties: Assist in development and implementation of a 5 and 10 year strategic plan for growth and development for the greater University of Arizona graduate and professional student council Appropriations Committee, Member Role: Graduate Student Committee Member Duties: Serve on a graduate student panel to evaluate and ensure the proper management and allocation of student fees paid to the graduate and professional student council Scientific Review Committee, Member Role: Graduate Student Committee Member Duties: Serve on a graduate student peer review panel to evaluate and score scientific applications for travel awards, professional opportunity and development awards The University of Arizona Graduate and Professional Student Council External Committees Faculty Senate Role: Graduate Student Representative Duties: Act as student liaison between the faculty at the University of Arizona and the graduate and professional student council, communicate the needs of graduate students, participate in decision-making, policy development, financial planning, and curricula development. Research Policy Committee Role: Graduate Student Representative
  • 13. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD Duties: Act as a student liaison communicating matters relating to research, conflict of interest, data retention, intellectual property, research ethics, research parks, interaction with industry, human and animal research, and safety Student Health Insurance Advisory Committee Role: Graduate Student Advisor Duties: Act as a student liaison communicating the needs of graduate students by negotiating insurance policies and benefits, advocating for increased benefits, organizing educational seminars, for graduate students and their families and assisting in website development and mass communication of insurance benefits. Student Services Fees Advisory Committee Role: Graduate Student Representative Duties: Act as a student liaison to evaluate communicate the needs of graduate students and ensure the proper management and allocation of student fees paid to the University of Arizona for IT and Library use Diversity Coalition Role: Graduate Student Representative Duties: Act as minority student liaison to between minority graduate students and faculty to communicate and assist in the strategic resolution of the needs and the challenges that face ethnic and demographic minorities in the graduate colleges at the University of Arizona MENTORING Affiliated Programs Biochemistry Program (BCP) 2011 - 2014 Role: Graduate Student Mentor Undergraduate Biology Research Program (UBRP) 2011 - 2014 Role: Graduate Student Mentor Initiative for Maximizing Student Diversity (IMSD) 2009 - 2014 Role: Graduate Student Mentor Keeping Youth Engaged in Science (KEYS) 2009 - 2010 Role: Graduate Student Mentor Students Trained Brian Weil (Duke University) 2015 - 2016 Jordan Bouldin (UNC) 2014 - 2015 Megan A. Neeb (UBRP) 2012 - 2014 Carmen Lee (BCP) 2011 - 2014 Quinea R. Lassater (IMSD) 2011 - 2014 Shivani Phanse (BCP) 2011 - 2013 Alexa Willamson (UBRP) 2010 - 2011 Ravi Ram (UBRP) 2010 - 2011
  • 14. Robert V. Brown, PhD 200 Adefield Lane, NC Rbrown842@icloud.com (321)-437-9016 http://www.linkedin.com/RVBrownPhD Forest L. Danford (UBRP) 2009 - 2011 Jessica Shelton (KEYS) 2009 - 2010 Jessica Kashien (IMSD) 2009 - 2010 Mathew R. Karolak (UBRP) 2009 - 2010 Misha Pangasa (BCP) 2008 - 2010 Vanessa C. Gaerig (BCP) 2008 - 2010 Lisette Rodriguez 2006 - 2008 Annan Kumar 2006 - 2008 Anthony J. Thomas 2006 - 2007 PROFESSIONAL & CHARACTER REFERENCES Terete Borrás, Ph.D. Affiliation: Collaborator, Character reference Phone: (919) 843-0184 Email: tborras@med.unc.edu Anton M. Jetten, Ph.D. (Please do not contact without prior consent from Robert V. Brown) Affiliation: PI, Postdoctoral Advisor Phone: (919) 541-2768 Email: jetten@niehs.nih.gov W. Daniel Stamer, Ph.D. Affiliation: Collaborator Phone: (919) 681-1566 Email: william.stamer@dm.duke.edu Caleb D. Sutherland, Ph.D. Affiliation: Collaborator, Character reference Phone: (919) 541-0189 Email: caleb.sutherland@nih.gov Laurence H. Hurley, Ph.D. Affiliation: PI, Predoctoral Ph.D. Advisor Phone: (520) 626-5622 Email: hurley@pharmacy.arizona.edu Tracy A. Brooks, Ph.D. Affiliation: Collaborator, Character reference Phone: (662) 915-2309 Email: tabrooks@olemiss.edu Daekyu Sun, Ph.D. Affiliation: Predoctoral Committee Member Phone: (520) 626-0323 Email: sun@pharmacy.arizona.edu