1. Bassam F Kashgari
555 N Commonwealth Ave., Fullerton, CA 92831, apt# 2163, (C) +1(937) 344-3799, bassam.kashgari@hotmail.com
Education:
o 2015 M.S. Degree in Microbiology & Immunology
Microbiology & Immunology M.S. program, Collage of Science & Mathematics
PI: Dr. Courtney Sulentic, Associate professor of Pharmacology & Toxicology,
Boonshoft School of Medicine
Wright State University, Dayton, OH, United States
GPA: 3.88 out of 4
Thesis: Determining the Role of the AhR in Immunoglobulin Expression and Class
Switch Recombination
Side projects: Using The Raman Technique to Detect the Human-IgH Looping in Ig
Production
o 2010 B.S. Degree In Biochemistry
Bachelor of Science, Biochemistry Dept.
PI: Dr. Samir Khoja, Professor of Biochemistry
King Abdul Aziz University, Jeddah, Saudi Arabia
GPA: 3.58 out of 5
Thesis: Asymmetric Dimethyl-L-Arginine With Renal Failure
Work Experiences:
o Graduate Researcher (Nov, 2013 - Sep, 2015)
Pharmacology & Toxicology Dept., Wright State University, Dayton, OH, United States
Designed experiments, optimized methodology, and established protocols for molecular
biology techniques: both real-time and qualitative PCR, Western blot, lentiviral transduction,
ELISA, and transient transfection by electroporation and luciferase gene reporters
Used lentiviral vectors to genetically modify B-cell lines, expanded and maintained gene-
modified B cell cultures, and characterized genetically modified B lymphocytes using
standard immunological assays that include western blot, ELISA, transient transfection, and
luciferase system
Worked with various B-cell lines for different species, isolated clones derived from a single
cell population, and measured cell count and viability using hemocytometer protocols
Trained and monitored less experienced graduate students in research techniques and ensure
they are adhering to protocol
Effectively planed and organized work activities to meet schedules and deadlines
Provided critical lab support while working in a collaborative team environment
Handled large amount of data, prepared reports, results, chart, graphs base on standardized
form, maintaned lab notebooks, and contributed to supportive documentation when
necessary
Became proficient in molecular cloning techniques: DNA amplification, separation,
purification, restriction digest, and preparing and analysis of DNA sequencing
Software analysis programs: GraphPad, Prism, CFX Manager, SOFTmax PRO analysis
software, MS-Office, and MS-outlook
o Undergraduate Medical Researcher Intern (Feb, 2009 - Apr, 2010)
King Fahd Centre for Medical Research, Jeddah, Saudi Arabia
Collected, labeled and stored blood samples in compliance with good clinical practice (GCP)
Measured protein expression using ELISA and flow cytometry
2. Gained hands on experience in spectrophotometer, HPLC, and chromatography
Maintained, set up, adjusted and operated laboratory equipment and instruments
Executed, analyzed, and recorded test results on standardized forms, prepared reports, results,
graphs and charts, and created and maintained database of subject information
Prepared chemical solutions and media according to standard formulas and calibrated
laboratory instruments
Ordered goods and supplies for the laboratory
o Laboratory Assistant Intern (Nov - Dec, 2012)
PepsiCo, Jeddah, Saudi Arabia
Managed goods and supplies databases
Preformed the chemical titration, dilution, solution, evaporation studies, pH studies, and
quality insurance
Prepared reports, results, graphs and charts
o Clinical Laboratory Assistant Intern (Aug - Oct, 2010)
Al-Sulimani Hospital, Jeddah, Saudi Arabia
Provided information for patient diagnosis and treatment by performing laboratory tests in
toxicology, chemistry, hematology, immunology, and microbiology laboratories; receiving,
typing, testing, and recording blood bank inventories
Served as technical resource by participating in staff training; answering questions of other
professionals; participating in educational opportunities; reading professional publications;
maintaining personal networks; participating in professional organizations
Preformed all laboratory tests and procedures, maintained quality results by running
standards and controls, calibrating equipment utilizing approved testing procedures, and
monitoring quality control measures and protocols
Seminars & Conferences:
International
o Society of toxicology 54th
annual meeting in San Diego (Mar 22-26, 2015)
Poster presentation about “Determining The role of the AhR in Class Switch
Recombination and IgH expression”
Regional
o Ohio Valley Chapter of the Society of Toxicology Annual Meeting (Sep 26, 2014)
Poster Presentation about ”The Mechanism of the AhR in The IgH Gene Expression”
Local
o Celebration of Research, Scholarship, and Creative Activities (April 10, 2015)
Oral presentation about ”Determining the role of the AhR in Class Switch
Recombination and IgH expression”
o Biomedical Sciences PhD Program Research Retreat (Aug 20, 2014)
Platform presentation about ”Determining the role of the AhR in Ig Expression”
o Celebration of Research, Scholarship, and Creative Activities (Apr 11, 2014)
Platform presentation about ”The Mechanism of the AhR in the IgH Gene Expression”
Professional Suciety Memberships:
o The Society of Toxicology (SOT)
o The Immunotoxicology Specialty Section (ITSS)
o Ohio Valley Society of Toxicology (OVSOT)
o Saudis in USA, Research and Science Students Section
3. Safety Trainings:
o Animal Occupation Health and Safety
o Radiation Generating Equipments
o Safe Use of Radioactive Materials
o Bloodborne pathogens
o Spill Prevention, Control, and
Countermeasure (SPCC) Rule
o Environmental Health & Safety
Technical Skills:
o Cell Culture
o Enyme-linked Immunosorbant Assay
o Western Blot
o RT-PCR
o qPCR
o Virul Transduction
o Transient Transfection
o Stable Transfection
o Flow Cytometry
Professional Skills:
o Project management to meet deadlines
o Proficient in data analysis softwares
o Public speaking
o International communication
o Strong work ethics
o Ambitious, optimistic, and eager to learn
o Documentation skills
o Laboratory management & organization
Career Development Courses:
o Leadership and management classes- Raj Soin Collage of Business, WSU
o Lab management classes, WSU
o General English Language classes, Education and Vocation Training
o Learning English as a Second Language- LEAP program, WSU
Community Services:
o Motivational speeches and guidance for post-graduate LEAP students at Wright State
University (2012-2015)
o Helping LEAP students with registration and introduction to the program at Wright State
University (2012-2014)
o Community outreach to K-12 students- presented laboratory techniques and discussed
science in general (2013)
o Guiding advisor in King Abdul-Aziz Airport (2008)
References:
Dr. Courtney Sulentic - Associate Professor of Pharmacology and Toxicology - Wright State University
+1(937)-755-3583 – courtney.sulentic@wright.edu
Dr. Nancy J. Bigley - Professor of Neuroscience, Cell Biology, and Physiology- Wright State University
+1(937)-755-2824 – nancy.bigley@wright.edu
Dr. Khalid Elasad - Professor of Pharmacology and Toxicology - Wright State University
+1(937)-755-2159 – khalid.elased@wright.edu
Dr. Katherine Excoffon - Associated Professor of Biology - Wright State University
+1(937)-755-2301 – katherine.excoffon@wright.edu
o Management for laboratory staff to ensure
employees are adhering to protocol and
resting procedure
o Conducting studies and litereture
searches
o Teaching and supervision of less expertise
o Ability to adapt oneself to new surroundings
o Hazardous Communication
o Waste Management
o Cold Weather Injury Prevention
o Asbestos Awareness
o Heat stress & Stormwater
o Heath & Food Safety
o Luciferase Assay
o Molecular Cloning
o Plasmid Construction
o RNA/DNA Extraction
o Protien Extraction
o Bacterial Transformation
o Electroporation
o Hemocytometer