Replication in Plant Viruses:
A Molecular Perspective
Plant viruses lead to significant crop losses, estimated at 15-20% globally.
Understanding their replication is crucial for developing robust disease
management strategies.
1. Virus multiply in living host
2.It takes energy from host Machinery
3.Virus spread its infection by the process of replication
Understanding Plant Viruses:
Structure and Diversity
Genetic Material &
Structure
Genetic Material:
Predominantly RNA (e.g.,
Tobacco Mosaic Virus -
TMV), less commonly DNA
(e.g., Geminiviruses).
Transmission
Routes
Transmission: Primarily
via insect vectors (>60% by
aphids), mechanical injury,
or infected seeds.
• Understanding these
fundamental
characteristics helps
us appreciate the
sophistication of plant
viruses.
Life cycle
virus completes its two life
cycle
1.Lytic cycle
Lysing means break or
burst of the cell(master
and slave cycle)
2.Lysogenic cycle
where the virus integrates
its genetic material into
host cell's DNA
Replication steps:
1.ATTACHMENT
This is the first step in virus infection in which interaction of virus with a
specific receptor site on the surface of host cell occurs.Virus attach
through tail fiber and make grip.
Viral protein on the capsid or phospholipid envelope interact with
specific receptors of the host.
Penetration:
After attachment there are some conformational change.leads to fusion
of viral and cellular membrane
As the host cell membrane performs ENDOCYTOSIS for entry of food
and water molecules. So virus also penetrate in the cell through
endocyctosis.
Uncoating and Genome Release
The capsid is removed , exposing the viral RNA/DNA.
The genome becomes accessible for replication and translation
Genome Replication
Strategies: DNA vs. RNA
Viruses
•
● RNA viruses: replicate using RNA- dependent RNA polymerase
•
● DNA viruses: use host's DNA polymerase or bring their own.
Transcription:
1. The process of transcription involves copying information from
strand of DNA into a new messenger RNA molecule(mRNA).DNA
preserves genetic material in the cells.
Translation:
A process by which viral mRNA is translated into viral protein using the host cellular machinery.The ribosome a structure
that is a factory for the creation of proteins is where translation takes place.
Assembly of New Virions:
Packaging the Genome
and movement.
The assembly of new virions is a fascinating display of molecular
precision. Once viral proteins are synthesised, the packaging process
begins.
•
● New virions are assembled in the cyt oplasm.
•
● Move to nearby cells via plasmodesmat a.
•
● Long- distance spread through phloem.
Conclusion
•
● Viral replication is host - dependent.
•
● Involves genome release, replication, translation, and spread.
•
● Import ant in plant disease managemen

Replication-in-Plant-Viruses-A-Molecular-Perspective (1).pptx

  • 1.
    Replication in PlantViruses: A Molecular Perspective Plant viruses lead to significant crop losses, estimated at 15-20% globally. Understanding their replication is crucial for developing robust disease management strategies. 1. Virus multiply in living host 2.It takes energy from host Machinery 3.Virus spread its infection by the process of replication
  • 2.
    Understanding Plant Viruses: Structureand Diversity Genetic Material & Structure Genetic Material: Predominantly RNA (e.g., Tobacco Mosaic Virus - TMV), less commonly DNA (e.g., Geminiviruses). Transmission Routes Transmission: Primarily via insect vectors (>60% by aphids), mechanical injury, or infected seeds. • Understanding these fundamental characteristics helps us appreciate the sophistication of plant viruses. Life cycle virus completes its two life cycle 1.Lytic cycle Lysing means break or burst of the cell(master and slave cycle) 2.Lysogenic cycle where the virus integrates its genetic material into host cell's DNA
  • 3.
    Replication steps: 1.ATTACHMENT This isthe first step in virus infection in which interaction of virus with a specific receptor site on the surface of host cell occurs.Virus attach through tail fiber and make grip. Viral protein on the capsid or phospholipid envelope interact with specific receptors of the host.
  • 4.
    Penetration: After attachment thereare some conformational change.leads to fusion of viral and cellular membrane As the host cell membrane performs ENDOCYTOSIS for entry of food and water molecules. So virus also penetrate in the cell through endocyctosis.
  • 5.
    Uncoating and GenomeRelease The capsid is removed , exposing the viral RNA/DNA. The genome becomes accessible for replication and translation
  • 6.
    Genome Replication Strategies: DNAvs. RNA Viruses • ● RNA viruses: replicate using RNA- dependent RNA polymerase • ● DNA viruses: use host's DNA polymerase or bring their own.
  • 7.
    Transcription: 1. The processof transcription involves copying information from strand of DNA into a new messenger RNA molecule(mRNA).DNA preserves genetic material in the cells.
  • 8.
    Translation: A process bywhich viral mRNA is translated into viral protein using the host cellular machinery.The ribosome a structure that is a factory for the creation of proteins is where translation takes place.
  • 9.
    Assembly of NewVirions: Packaging the Genome and movement. The assembly of new virions is a fascinating display of molecular precision. Once viral proteins are synthesised, the packaging process begins. • ● New virions are assembled in the cyt oplasm. • ● Move to nearby cells via plasmodesmat a. • ● Long- distance spread through phloem.
  • 10.
    Conclusion • ● Viral replicationis host - dependent. • ● Involves genome release, replication, translation, and spread. • ● Import ant in plant disease managemen