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Rapid multiplication
system in the greater
Mekong subregion
12 September, 2019
Vientiane, Lao P.D.R.
Erik Delaquis & Roosevelt Escobar
e.delaquis@cgiar.org
r.escobar@cgiar.org
Contents
- Context & background
- Rationale
- CIAT’s cassava seed system approach
- Tunnel-based rapid multiplication system
Context & background
- 100+ years of ‘disease-free’ multiplication,
evolution of complex regional farmer network
- ‘Formal’ system: government, research &
extension players
- Different models of production, dissemination
- Levels of subsidy – no strictly business models
Delaquis et al., 2018
Context & background
- Old goal of multiplication:
- New varieties -> farmers ->  production
- New goals for multiplication:
- Varietal identity control
- Phytosanitary control
- Product quality control / market differentiation
Thai cassava variety distribution (2017) - OAE
Rationale
- Preliminary results (CAVAC): clean planting
material can defend yield penalty from CMD
infection (at least in year 1)
- Laws limiting transport of untested materials,
but no sources of clean material available in
most countries/settings
- Need for increased capacity to rapidly
multiply resistant materials in the pipeline
Seed multiplication models
- Stem multiplication pathways
- Need for increasing multiplication rates,
lowering cost per unit
New variety
Redistribution
Tissue
culture
Greenhouse /
screenhouse
Open field
multiplication
Virus
testing
‘Conventional’ cassava multiplication pathway
- Relies on repeated open field
multiplication cycles
- Low 1:10 multiplication rate / year; very
slow to multiply new material
- No phytosanitary control in major
multiplication stages
- In reality…
In vitro
Distribution
Isolated field
(greenhouse)
4 Multiplication
tunnels
Isolated field
multiplication
Virus
testing
3-4 cycles per year
1:10 multiplication rate
10 ha planting material / cycle
10,000 plants / ha
1,000,000
conventional stakes
Tunnel based rapid stem multiplication (4 tunnels + 10 ha field)
500 clean
mother plants
10 Ha field plan for stem multiplication (4 tunnels)
In vitro
Distribution
Isolated field
(greenhouse)
4 Multiplication
tunnels
Isolated field
multiplication
- Start with 1000
tested in vitro
plants
- 500 mother plants
maintained in
clean greenhouse
- Rapid multiplication tunnels
increasing multiplication
rate with cycle time 3
months
- Production in each year of
10 ha planting material
Tunnel system: speeding up scaling
Inexpensive, major increase in multiplication rate
Uses immature sprout cuttings instead of conventional stakes, speeding up multiplication
Each tunnel can produce material for 0.75-1 Ha / year
How does the system work?
Using immature tissues (cuttings and branches*)
Material on field conditions with
agronomic & practical management
In most of Asian clones is not occurs many branches. It works for Latin American clones
Timeline for tunnel multiplication – 4 tunnel system for 10 ha
Year 1 Year 2 Year 3
Tunnels cumulative output (plants) 100,000 200,000 300,000 Guaranteed clean
Isolated field material (plants) 1,000,000 2,000,000 3,000,000 1st round isolation field
Secondary field multiplication (farmer) 10,000,000 20,000,000 30,000,000 2nd round farmer field
Tunnel multiplication
STAGE Month
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
Receiving plantlets
In-vitro multiplication
Hardening
Clean field (nethouse)
Tunnel 1
Tunnel 2
Tunnel 3
Tunnel 4
STAGE Month
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
Receiving plantlets
In-vitro multiplication
Hardening
Clean field (nethouse)
Tunnel 1
Tunnel 2
Tunnel 3
Tunnel 4
STAGE Month
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40
Receiving plantlets
In-vitro multiplication
Hardening
Clean field (nethouse)
Tunnel 1
Tunnel 2
Tunnel 3
Tunnel 4
Summary
- Tunnel system advantages:
- Greatly shortens time to increase available planting material
- Increases annual multiplication rate through use of miniset and immature cuttings
- Could implement a QC program
- Tunnel system can support releases of new planting material
- Required infrastructure:
- Centralized greenhouse for mother plants
- Set of 4 multiplication tunnels
- Electric/solar power, water supply
- 8 staff & manager
- 10 ha fields for first round multiplication (pre-basic seeds)
Considerations for discussion groups
- Clones (by level of resistance &
agronomic performance) to put into
the system
- Location of centralized greenhouse
facilities (mother plants)
- Number & location of field systems,
considering strategy for dissemination
- Evaluation of existing facilities, in vitro
varieties (where, #, QC)
- Future: dissemination strategies,
dealer concept and
tracking/monitoring
- Training & capacity building
Thank you!

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Rapid multiplication systems in the greater Mekong subregion

  • 1. Rapid multiplication system in the greater Mekong subregion 12 September, 2019 Vientiane, Lao P.D.R. Erik Delaquis & Roosevelt Escobar e.delaquis@cgiar.org r.escobar@cgiar.org
  • 2. Contents - Context & background - Rationale - CIAT’s cassava seed system approach - Tunnel-based rapid multiplication system
  • 3. Context & background - 100+ years of ‘disease-free’ multiplication, evolution of complex regional farmer network - ‘Formal’ system: government, research & extension players - Different models of production, dissemination - Levels of subsidy – no strictly business models Delaquis et al., 2018
  • 4. Context & background - Old goal of multiplication: - New varieties -> farmers ->  production - New goals for multiplication: - Varietal identity control - Phytosanitary control - Product quality control / market differentiation Thai cassava variety distribution (2017) - OAE
  • 5. Rationale - Preliminary results (CAVAC): clean planting material can defend yield penalty from CMD infection (at least in year 1) - Laws limiting transport of untested materials, but no sources of clean material available in most countries/settings - Need for increased capacity to rapidly multiply resistant materials in the pipeline
  • 6. Seed multiplication models - Stem multiplication pathways - Need for increasing multiplication rates, lowering cost per unit
  • 7. New variety Redistribution Tissue culture Greenhouse / screenhouse Open field multiplication Virus testing ‘Conventional’ cassava multiplication pathway - Relies on repeated open field multiplication cycles - Low 1:10 multiplication rate / year; very slow to multiply new material - No phytosanitary control in major multiplication stages - In reality…
  • 8. In vitro Distribution Isolated field (greenhouse) 4 Multiplication tunnels Isolated field multiplication Virus testing 3-4 cycles per year 1:10 multiplication rate 10 ha planting material / cycle 10,000 plants / ha 1,000,000 conventional stakes Tunnel based rapid stem multiplication (4 tunnels + 10 ha field) 500 clean mother plants
  • 9.
  • 10. 10 Ha field plan for stem multiplication (4 tunnels) In vitro Distribution Isolated field (greenhouse) 4 Multiplication tunnels Isolated field multiplication - Start with 1000 tested in vitro plants - 500 mother plants maintained in clean greenhouse - Rapid multiplication tunnels increasing multiplication rate with cycle time 3 months - Production in each year of 10 ha planting material
  • 11. Tunnel system: speeding up scaling Inexpensive, major increase in multiplication rate Uses immature sprout cuttings instead of conventional stakes, speeding up multiplication Each tunnel can produce material for 0.75-1 Ha / year
  • 12. How does the system work?
  • 13. Using immature tissues (cuttings and branches*) Material on field conditions with agronomic & practical management In most of Asian clones is not occurs many branches. It works for Latin American clones
  • 14. Timeline for tunnel multiplication – 4 tunnel system for 10 ha Year 1 Year 2 Year 3 Tunnels cumulative output (plants) 100,000 200,000 300,000 Guaranteed clean Isolated field material (plants) 1,000,000 2,000,000 3,000,000 1st round isolation field Secondary field multiplication (farmer) 10,000,000 20,000,000 30,000,000 2nd round farmer field Tunnel multiplication STAGE Month 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Receiving plantlets In-vitro multiplication Hardening Clean field (nethouse) Tunnel 1 Tunnel 2 Tunnel 3 Tunnel 4 STAGE Month 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Receiving plantlets In-vitro multiplication Hardening Clean field (nethouse) Tunnel 1 Tunnel 2 Tunnel 3 Tunnel 4 STAGE Month 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Receiving plantlets In-vitro multiplication Hardening Clean field (nethouse) Tunnel 1 Tunnel 2 Tunnel 3 Tunnel 4
  • 15. Summary - Tunnel system advantages: - Greatly shortens time to increase available planting material - Increases annual multiplication rate through use of miniset and immature cuttings - Could implement a QC program - Tunnel system can support releases of new planting material - Required infrastructure: - Centralized greenhouse for mother plants - Set of 4 multiplication tunnels - Electric/solar power, water supply - 8 staff & manager - 10 ha fields for first round multiplication (pre-basic seeds)
  • 16. Considerations for discussion groups - Clones (by level of resistance & agronomic performance) to put into the system - Location of centralized greenhouse facilities (mother plants) - Number & location of field systems, considering strategy for dissemination - Evaluation of existing facilities, in vitro varieties (where, #, QC) - Future: dissemination strategies, dealer concept and tracking/monitoring - Training & capacity building