1. Fresh fish quality is assessed using sensory and non-sensory methods to determine freshness and detect spoilage. Sensory methods rely on human panels while non-sensory methods include biochemical, biological, and physical tests.
2. Processing fresh fish prevents spoilage through methods like removal of scales, filleting, and packaging. Common fish products include fish sauce, fish paste, fish oil, and fish protein concentrate created through various extraction and fermentation processes.
3. Fish protein concentrate is created through a multi-step extraction process using solvents to remove oils and concentrate proteins from fish or fishery waste to over 85% protein content.
1. QUALITY OF FRESH FISH
SUBMITTED BY
SAKSHI THAKUR
1732065
M.Sc. FOOD SCIENCE AND TECHNOLOGY
2. INTRODUCTION
• Freshness describes the presence and absence of deterioration.
• Fish is highly perishable food.
• Spoilage in fish starts as soon as the fish dies after fishing.
• Freshness makes major contribution to the quality of fish or fishery products.
• The detection of freshness of foods has become an important routine in the fields of food industry.
• There are different methods used for the assessment of fish quality
3. METHODS FOR QUALITY OF FISH
SENSORY METHOD
• Based on changes in appearance, odour, colour, flavour and texture.
• Fast, simple, sensitive and objective.
• It rely on human judgment and proper training of panels.
• Instrument like Instron, Bohlin Rheometer for measuring texture and other rheologic properties are used and articial
nose to evaluate odour.
• Some sensory methods used are
QUALITY INDEX METHOD (QIM)
TORRY SENSORY ASSESMENT SCHEME
4.
5. NON SENSORY METHOD
• Can be cheaper and often quicker.
• Can appear more reliable and objective.
• Courts of law may find it much easier to accept the results of physical and chemical tests.
• There are different non sensory methods :
• BIOCHEMICAL METHOD
A variety of chemical compounds accumulate post-mortem fish muscles and these chemical are either intermediate or end products
of biochemical changes occurring in muscles after death.
a. Proximate Composition : For the determination of moisture, ash, lipid, protein of fish sample. It varies with species, age, sex, season etc. So
conclusive result are difficult to obtain
b. Histamine content: Histamine develops in freshly caught fish after 40-50 hrs of death, if fish is not properly iced. Histamine content over 20
mg/100g in canned fish is prohibited by US. FDA.
c. pH: Changes in fish muscle is a good index for freshness assesement.
d. Ammonia: Bacteria can generate small amount of ammonia in spoiling fish, mainly from free amino acid. There are several chemical and
enzymatic method for measuring ammonia.
e. Peroxide value: Use to determine oxidative rancidity in fish tissue. Good quality fish have value less than 10.
6. • BIOLOGICAL METHOD
a. Total Plate Count: Total number of microbial flora is changed with the time in fish or fish products. The
number per grams of fish and fish products or per square centimetre of surface area indicate quality of fish
from microbiological point of view.
• PHYSICAL METHOD
7. PROCESSING OF FISH
• The purpose of processing and preserving fish is to get fish to an ultimate consumer in good, usable
condition.
• Fish that becomes spoiled is obviously unusable.
• The aim of fish processing and preservation is to slow down or prevent the enzymatic, bacterial, and
chemical deterioration, and to maintain the fish flesh in a condition as near as possible to that of fresh
fish.
• Steps include in the processing of fish are:
9. FISH PRODUCTS
• There are different products of fish:
• FISH SAUCE
• FISH PASTE
• FISH OIL
• FISH PROTEIN CONCENTRATE
10. FISH PASTE (BAGOONG)
• Bagoong is undigested residue of partially hydrolysed fish or shrimp.
• It has salty and slightly cheese-like odour.
WASHED AND DRAINED
MIXING OF SALT AT DIFFERENT PROPORTIONS
MIXTURE IS ALLOWED TO FERMENT
• Bagoong is eaten raw or cooked.
• Used as flavouring or condiment in many traditional recipies.
• As an appetizer it is sautéed with onion and garlic and served with tomatoes or green mangoes.
• Main source of protein in the diet.
11. FISH SAUCE (PATIS)
• The raw material used is similar to that of fish paste.
• They differ only with respect to the period of fermentation.
• To obtain fish sauce the fermentation is continued until liquid forms on top of the mixture, after
which it is drained and filtered.
• Fish sauce may be obtained either from fish or shrimp bagoong after 1-2 years of fermentation.
• The longer the digestion period, the better.
• The total bacterial counts decreased rapidly upto 6 month and declined slightly until the end of
fermentation.
12. FISH PROTEIN CONCENTRATE (FPC)
• FPC usually refers to fish meal intended for human consumption.
• It can be prepared from any type of fish or fishery waste.
• It is prepared from fish by extracting out the oil, screening or settling out the bones and drying, so
that the resultant product (Fish Protein Concentrate) is higher in protein *85% to 95 % and lower in
ash content than fish meal.
• The Food and Agriculture Organization of the United Nations defines three types:
•
Type A: a virtually odourless and tasteless powder having a
maximum total fat content of 0·75 per cent.
Type B: a powder having no specific limits as to odour or
flavour, but definitely having a fishy flavour and a
maximum fat content of 3 per cent.
Type C: normal fish meal produced under satisfactorily
hygienic conditions
13. 1. Fresh whole fish are rinsed with fresh water soon after landing, weighed and fed
to a mincer by conveyor.
2. First extraction: the minced fish are fed to extractor 1 which dehydrates the fish;
it is an unheated vessel in which the mince is agitated for about 50 minutes
together with the liquid recovered from extractor 2, which contains some
isopropanol.
3. Centrifuging: the contents of extractor 1 are fed to a continuous centrifuge,
where the slurry separates into a solid known as wet cake, and a liquid. The wet
cake is conveyed to extractor 2, and the liquid to a still for recovery of solvent and
fat.
4. Second extraction: extractor 2 is jacketed, and the temperature is about 75°C.
Here the liquid recovered from extractor 3 is added to the wet cake from extractor
1 and the mixture is agitated for 90 minutes. At the beginning of this stage the cake
is almost completely dehydrated, but has a fat content of about 5 per cent, which is
reduced to about 1 per cent during the extraction.
14. 5.Centrifuging: the contents of extractor 2 are centrifuged, the wet cake is conveyed to
extractor 3, and the liquid is returned to extractor 1 for the next batch of raw material.
6.Third extraction: extractor 3 is jacketed, and the temperature is again about 75°C.
Fresh isopropanol is added to the wet cake and agitated for about 70 minutes. During
this stage the fat content is reduced to about 0·3 per cent.
7. Centrifuging: the contents of extractor 3 are centrifuged and the wet cake is washed
with pure isopropanol for about 50 minutes. The liquid is returned to extractor 2 for
the next batch.
8. Solvent removal: the wet cake is heated in a rotating vacuum dryer to evaporate the
solvent; the vapours are drawn off, condensed and used again.
9. Grinding and packing: the dried material is conveyed to a hammer mill, where it is
ground to a fine powder and sieved. The FPC is typically packed in 50-lb fibreboard
containers and sent to store ready for shipment.
15. REFERENCES
• www.ncbi.nlm.nih.gov.in
• www.fao.org
• Solanki J, et al., (2016), Freshness evaluation of fish by quality index method (QIM) and instrumental
method at Veraval Fish Landing Centre, International Journal of Processing and Post Harvest
Technology, 42-46.
• Bremner, H.A., (2002), Safety and quality issues in fish processing, Woodhead Publishing Limited,
607-625.
• Nwaigwe U.V., (2017), Fish preservation and processing, Journal of food.
Editor's Notes
Torry freshness score shows the state of fish freshness. The scoring system starts from 10 and declining. 10 is highest score for newly caught fish 7 is in neutral range 6 is borderline 3 or less is considered spoiled. . Evaluation of result generally shows th presence of m/o which cause spoilage ,ie, lower score shows more presence of m/o
QIM IS SECOND METHOD FOR SENSORY EVALUATION… QIM GIVES 0 SCORE FOR THE FRESH FISH…LARGER NO. SHOWS SPOILAGE OF FISH.
Histamine is major prob in warm water species that cause a form of food poisoning.
The amount of ammonia gives indication, though not accurate one, but some extent of spoilage. Much larger amnt of ammonia are produce during the spoilage of some fish like dogfish, skate etc bcs the have larger amnt of urea in their flesh.
Main problem with the quality of fish is rancidity due to oxidation. PEROXIDE VALUE above 20 is considered as rancid.
TPC IS DETERMINED BY CULTURING THE BACTERIA IN SUITABLE CULTURE MEDIAWHICH RECOVER MAXIMUM number of bacteria in fish tisuue.
Electrical properties of fish skin and muscle changes after death so it can be determine by using the instrument like torrymeter. Changes in electrical prop is not due to the action of bacteria or any spoilage mechanism but it can correlate with stage of spoilage. Instrument used only on whole fish or fillet with skin. Frozen fish when thawed give no response to meter.
Handling of fish : When fish are captured or harvested for commercial purposes, they need some preprocessing so they can be delivered to the next part of the marketing chain in a fresh and undamaged condition. This means, for example, that fish caught by a fishing vessel need handling so they can be stored safely until the boat lands the fish on shore. Typical handling processes are (FAO, 2011): transferring the catch from the fishing gear (such as a trawl, net or fishing line)
to the fishing vessel
holding the catch before further handling
sorting and grading
bleeding, gutting and washing
chilling
storing the chilled fish
unloading, or landing the fish when the fishing vessel returns to port
Removal of scales: or a whole flat fish, wash and cut off the head. Holding the fish by the tail and using
a sharp knife, Scale it by scrapping toward the head. Scrap until all the scales are
removed (UNDFFW, 2003). Turn the fish and scale the other side.
Cutting: Cutting the removal of the guts (intestines) of the fish. Gutting should be carried out
on fish no matter what method of preservation is going to be applied. After gutting,
the fish should be washed thoroughly with clean running water. Gutting and washing f the fish helps to prevent bacterial attack before and during processing,
preservation and storage
Filleting of fish: The processing industry also adopted freezing of fish in the form of fillets at times when prawns are not available. Fillets are nothing but the strips of flesh cut parallel to the backbone of the fish. Fishes like milk fish, cat fish, perches, mullets, carps, eel, etc (Bekker-Nielsen, 2005) are suitable for filleting and freezing (Luten et al.,
2006). Filleting can be done by hand which is economical or by using a filleting machine. Fillets may be with or without skin and it fetches a much higher price in
the luxury market. Fillets are dripped in brine to enhance their appearance and to reduce the amount of drip and it also gives a salty flavor (Bekker-Nielsen, 2005). The freezing of fillets can be an individual quick freezing of block freezing (Zohar et al., 2001). After dropping in brine, the fillets wrapped in polythene sheet are frozen in contact
plate freezer at -350C to 400C. In block freezing the fillets in known weight 500grm, 1Kg, 2Kg. are packed in polythene bags lined with wax and sufficient quantity of
glazed water is poured to cover the fillets (Bremner, 2003). The fillets are put in a freezer at -35 to 40 C and stored at -23 C.
Fish used for bagoong are sardines, herring, shrimp. Slipmouth, oyster, clams.Salt mixed with drained samples at different proportions e.g. 1:3 or 2:7 depend on bulk of preparation.Mixture is allowed to ferment for several months or longer until it develops characteristic flavour and aroma of bagoong. Some studies shown that total viable count decrease with time.
The important point to note is that the solid FPC material and the solvent move through the extractors in opposite directions. Thus the solvent becomes more contaminated with water and fat as it moves from extractor 3 to extractor 1, while the FPC loses water and fat as it moves the other way. The fat can be recovered from the solvent, and the solvent can be used again; solvent losses are said to be only about 1 per cent of the amount used for a batch. First, of course, because it is concentrated; untreated and unprocessed foods do not generally contain more than about 20 percent protein, whereas FPC contains about 80 per cent. Secondly, the quality of the protein is high; by this is meant that the amino acids which make up the protein are present in just the right balance for human nutrition. Other foods such as cereals may contain useful amounts of protein but are frequently deficient in one or more of the amino acids that are essential for growth.