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Additional Information
Promoter tools for further development of Aspergillus oryzae
as a platform for fungal secondary metabolite production
Maiko Umemura, Kaoru Kuriiwa, Linh Viet Dao, Tetsuya Okuda, and Goro Terai
1 kb
Fusion PCR
pyrG
5’-UTR
ustR.5F
ustR.5R
pyrG.F
pyrG.R
ustR.3F
ustR.3R
p0X.F
p0X.R
5’-UTR
Transformation
of A. oryzae
Figure S1. Construction of transformants for promoter activity test
ustR
Promoter
pyrG
5’-UTR ustR
Promoter
ustR
1 kb 1 kb
1.9 kb
Figure S2. Fungal cell weight after 10 days in V8 and 20 days in PDB liquid medium culture

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Promoter tools for further development of Aspergillus oryzae.pptx