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Amanda Icazatti wants to pursue an MD-PhD degree to assist people through both medical knowledge and advanced scientific research. She is interested in areas like cytology, genetics, and regenerative biology that could help understand and cure diseases. An MD-PhD would allow her to directly help patients while also conducting research to develop new treatment techniques and standards of care. She ultimately aims to contribute to scientific advances and improvements in human health and welfare through biomedical research.
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Behavioral pharmaceutical is additionally a moderately new idea according Julio Licinio. It is an interdisciplinary field of study consolidating learning from fields like science, brain research and sociologies.
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1. The document discusses different worldviews on health, illness, healthcare, and wellness from Western, Eastern, African, and South Asian perspectives.
2. In the Western view, health is seen through a biomedical lens focused on the human body. Illness is viewed as the result of natural phenomena. Healthcare involves evidence-based treatment using modern medicine. Wellness encompasses proper physical functioning of the body's systems.
3. The Eastern perspective views health holistically as a balance of body, mind, and spirit. Illness results from imbalances that can be treated by restoring balance. Healthcare considers individual diagnosis and restoring chi (energy) through natural remedies. Wellness requires harmony within oneself and with one
Successful grant proposal (University of Arizona Honors College)
Richard Alan Russell proposes research to be conducted in Oaxaca, Mexico under the supervision of Dr. Downing from the University of Arizona. The research aims to understand how individuals of different socioeconomic statuses value traditional and Western medicine systems in Oaxaca. Russell will conduct interviews and surveys to analyze how wealth and education relate to views of the different treatment types. He will spend the first month improving his Spanish and medical terminology at a language institute. The second month will involve qualitative research methods like interviews and questionnaires with samples from private, public, and traditional healthcare sectors. The research seeks to provide an insider perspective on healthcare definitions in Oaxaca.
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Reflexion essay
This essay reflects on the author's experience in a biology seminar course throughout the spring 2014 semester. The course focused on training undergraduates in biomedical research techniques. Students conducted laboratory work in fields like biology, biochemistry, physics, and chemistry. They isolated and characterized bacteriophages found in tropical soils. The author specifically worked with isolating and purifying a bacteriophage named Gemeos. Additional experiences included isolating plasminogen activator from HeLa cells and learning about electrospinning and nanotechnology. The course helped develop the author's understanding of scientific research and their ability to communicate their work. It served to prepare the author for an upcoming research experience at North Carolina State University that summer.
Criterion
As a young girl, the author had problems communicating effectively and would express herself without considering others or accepting criticism. After a significant mistake on a school math report caused problems, she began reflecting on how to improve. She now analyzes what she wants to say before speaking and accepts feedback from others, believing others can provide valuable perspectives. The author has learned the importance of communicating respectfully and understanding different views as part of personal growth.
Creative writing rise
Diego visited Ancient Greece and sketched many wonders, including monuments built by Greek gods, buildings made of stone and clay, and statues of gods made of gold. In his sketches, he depicted a large wall built by Vikings during wars between northern and southern Vikings, as well as the pyramids of Greece, which the Egyptians built first but on a larger scale. He also saw the Mountains of Zeus, created to protect villages from demons, and Dimensions Lake, said to have a portal to a world of dreams.
Creative story for competition rise
Harold, a mouse, narrates his experience being taken from his home and family to be used in a laboratory experiment. The experiment involves removing a gene that causes mice to overeat uncontrollably and develop health issues like obesity and diabetes. Harold watches as his friend Eugene undergoes the experiment and transforms into an unrecognizable beast. Harold fears undergoing the experiment himself and questions why humans subject animals to such experiments without consent when they would not do the same to their own families. In his final words, Harold expresses that what was done to him and the other mice is unfair and asks when humans will realize the harm of these types of experiments.
Final solar disinfection for water purification
The document describes the SODIS (Solar Disinfection for Water Purification) method for purifying water using sunlight. The method involves filling PET bottles with contaminated water and exposing them to sunlight for 6 hours, as the UV rays are supposed to kill any bacteria. The study aimed to test this method using a UV light machine instead of sunlight. Water samples were exposed to UV light for varying times, but results showed no decrease in bacterial contamination, as coliphage concentrations remained similar regardless of exposure time. This suggests the UV light machine may not have been intense enough or a longer exposure time was needed to effectively disinfect the water.
Final SODIS Method
The document describes the SODIS (Solar Disinfection for Water Purification) method for purifying water using sunlight. The method involves filling PET bottles with contaminated water and exposing them to sunlight for 6 hours, as the UV rays are supposed to kill any bacteria. The study aimed to test this method using a UV light machine instead of sunlight. Results showed equal levels of bacterial contamination in both treated and untreated water samples, suggesting the UV light machine was not strong enough or the exposure time was too short to effectively reduce bacteria as expected using the SODIS method.
Final Bacteriophages
The researchers isolated a novel bacteriophage from tropical soil samples in Brazil and named it Gêmeos. Through enrichment culture and purification techniques, they isolated the phage that infected Bacillus cereus. Initial observations indicated Gêmeos was a lysogenic phage. Electrophoresis of the phage's proteins showed migration, indicating successful separation. Further experiments like genome sequencing could provide more details about Gêmeos.
Experiments with membranes
Cells contain organelles that perform specific functions and are surrounded by a cell membrane composed of a phospholipid bilayer and proteins. The membrane acts as a barrier while also facilitating transport of molecules via osmosis and selective transport. An experiment uses water droplets surrounded by oil to model a cell membrane, with some droplets containing salt to demonstrate osmosis and others dyed to show selective transport. The materials needed, abbreviated as the MOST kit, include a USB microscope, platform, and chip to hold the droplets and observe membrane behavior. Studying membrane function could help develop therapies for membrane-related health issues.
Protein isolation and page electrophoresis
The document discusses protein isolation and polyacrylamide gel electrophoresis (PAGE). Plasminogen activator, an enzyme that prevents blood clotting, is being isolated from Hela cells using magnetic nanoparticles. Chromatography and zymography help determine the presence and activity of proteins. PAGE was then used to separate the isolated proteins by size. During the experiment, it was found that one of the reactants was contaminated based on abnormal absorbency readings.
Protein isolation and page electrophoresis
The document discusses protein isolation and polyacrylamide gel electrophoresis (PAGE). Plasminogen activator, an enzyme that prevents blood clotting, is being isolated from Hela cells using magnetic nanoparticles. Chromatography and zymography help determine the presence and activity of proteins. PAGE was then used to separate the isolated proteins by size. During the experiment, it was found that one of the reactants was contaminated based on abnormal absorbency readings.
Water sampling and testing
Water is essential to life but can become contaminated, posing health risks. The document describes testing water samples from Puerto Rico for coliphages, which would indicate contamination from animal feces. No coliphages were found, suggesting the water was safe, though errors are possible since no positive results occurred. A positive result would mean the water contained animal waste and active, harmful bacteria like Salmonella, making it unsafe for use.
Microscopy and photomicrography
Microscopy and Photomicrography provides an overview of different types of microscopes and their uses. There are various kinds of microscopes that are used for different purposes depending on the specimen being studied. Examples include phase contrast microscopy for living specimens without dyes, dissecting microscopes for examining 3D specimens, and bright field and dark field microscopes for contrasting unstained samples. Students were taught how to use specific microscopes and served as mentors to teach their partners, with the author learning bright field microscopy and their partner teaching phase contrast microscopy. Understanding different microscopy techniques provides valuable knowledge that can be applied across science courses and careers.
6.amanda2nd workshopsummary23andahalfptsrevised corrected
The document summarizes a laboratory workshop on microscopy techniques. It discusses several types of microscopes, including bright field, phase contrast, Normaski, and dissecting microscopes. It explains that each microscope is used for different purposes and specimen preparations. As part of the workshop, students learned how to use specific microscopes and taught their partners. The summarizer learned bright field microscopy and was taught phase contrast by their partner. Knowing various microscopy techniques provides scientists different perspectives to further understand biological processes.
Microscopy and photomicrography
Microscopy and Photomicrography provides an overview of different types of microscopes and their uses. There are various kinds of microscopes that are used for different purposes depending on the specimen being studied. Examples include phase contrast microscopy for living specimens without dyes, Normaski microscopy for transparent samples, and dissecting microscopes for examining three-dimensional specimens. The document also discusses different microscopy techniques for sample preparation, such as transverse, longitudinal, tangential, and cross sections. As part of a learning exercise, students were paired to teach each other how to use different microscopes, such as bright-field and phase contrast microscopy. Mastering various microscopy skills and techniques provides valuable knowledge that can be applied across science courses and
Nanotechnology
Nanotechnology involves manipulating materials at the nano scale of 10-9 meters to reduce energy consumption and improve properties. Electrospinning uses high voltage to create fibers from liquid solutions that can be used for gas sensing and protein separation. Scanning electron microscopy allows viewing particle arrangements at high magnification to characterize samples and understand their behavior. Nanoparticles show promise in less invasive medical treatments like joint implants due to their catalytic properties enabling faster response times and more targeted treatments.
Barcoding and nemerteans
Scientists use DNA barcoding to identify organisms through sequencing genetic material, which provides a precise global technique. Procedures like PCR and electrophoresis are used to amplify and analyze DNA fragments to better understand species. Studying organisms like nermerteans, which can regenerate their nervous system, shows how science works to resolve unknowns and make the world more interesting to explore.
Presentationrise amib
Two bacteriophages, Gêmeos (a bacilophage) and Isinaayusferti (a mycobacteriophage), were isolated from soil samples in Brazil and Puerto Rico, respectively. Gêmeos was found in dark, damp soil near a river in Sao Paolo, Brazil, while Isinaayusferti was discovered in humid soil near garbage in Caguas, Puerto Rico. Through enrichment and purification steps, high titer lysates of both bacteriophages were obtained.
SODIS Method
The document discusses the Solar Disinfection (SODIS) method for water purification. SODIS uses UV rays from sunlight to kill pathogens in water by filling PET bottles with water and exposing them to sunlight for 6 hours. The document outlines how SODIS works, important details like how recontamination is prevented and challenges with turbidity. It then describes testing a water sample from Rio La Plata, Cayey for coliphages using SODIS with a UV light machine. The results found the method was not effective with the machine. Future work would repeat using sunlight and test for Enterococcus.
Curriculum Vitae
Amanda M. Icazatti Burtell is a Biology student at the University of Puerto Rico - Cayey Campus who is expected to graduate in May 2016. She has experience in bacteriophage research through programs like PHAGES and RISE. Her coursework includes Genetics, Botany, Zoology, and Chemistry. She has skills in teamwork, communication, computers, and sign language. Her research experience includes isolating and characterizing bacteriophages from soil samples in Puerto Rico. She has presented her research at conferences and received honors and scholarships for her academic performance.
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Rise 22.1
- 1. Career plan by Amanda Icazatti When I begin to think about what I want to become and do when I graduate with a bachelor degree from the university, many things come to my mind. I want to contribute to my country, Puerto Rico and to the World. I have always had interest for the well-being of people. Being exposed to science courses so far during my bachelor degree, has instilled in me a broad view and understanding about the capabilities of science and all the things it has to offer for society, (and I know there is still a world for me to discover). When I began to explore possibilities and the different things I could study, an MD-PHD is the nearest to my interests. The reason that I am interested in having such a degree is because I will be able to assist people, not only with my knowledge in medicine, but with more advanced knowledge in a specific area of science. What would be interesting is that I will be able to solve patient’s struggles and at the same time do research looking for a way to treat and cure the person’s disease. I will also have the opportunity and possibility to translate my research into a way and a standard technique to be used by others to cure a disease that was being researched. I am very interested in areas such as Cytology, Genetics, and Regenerative Biology. I believe that these are all complementary branches of science that would help in the understanding of diseases, their managements, and even provide tools for the development of therapies. In addition, these are all areas of active research that can provide with answers unknown conditions in a person’s life. Biomedical research promises to be an area of great development for the welfare and health of the human race.