URBAS ASHIQ presentation on bacterial biofilms
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AASALAMU ALIKUM dea friends... i am urbas ashiq...(MSC Microbiology) i am hear to get help and help you ghyz regarding the knowledge of microbiology and other related fields
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Microbial biofilms formation
There are many studies about bacterial and fungal biofilm which they were considered a very big problem now days ,because of that it was one of the most virulence factors which in turns increased resistant for antibiotics
Bacterial biofilms are complex surface attached communities of bacteria held together by self-produced polymer matrixs mainly composed of polysaccharides, secreted proteins, and extracellular DNAs
A biofilm can consist of a single microbial species or a combination of different species of bacteria, protozoa, archaea, algae, filamentous fungi, and yeast that strongly attach to each other and to biotic or abiotic surfaces
bacterial biofilm formation relies on the interaction between the bacterial cells, the substrates and the surrounding media . And the formation of bacterial biofilms is a multi-step process starting with reversible attachment to surfaces aided by intermolecular forces and hydrophobicity, and then progress to extracellular polymeric substances (EPS) production which enable the cells to permanently adhere to a surface.
there are five main phases involved in the biofilm formation process:
reversible attachment
irreversible attachment
EPS production
maturation of biofilm
dispersal/detachment
Microbial biofilms formation
Formation of microbial biofilms preparing by:
Assist. Lect. Aysar Ashour Khalaf
There are many studies about bacterial and fungal biofilm which they were considered a very big problem now days ,because of that it was one of the most virulence factors which in turns increased resistant for antibiotics . Bacterial biofilms are complex surface attached communities of bacteria held together by self-produced polymer matrixs mainly composed of polysaccharides, secreted proteins, and extracellular DNAs
Bacterial biofilms are complex surface attached communities of bacteria held together by self-produced polymer matrixs mainly composed of polysaccharides, secreted proteins, and extracellular DNAs
A biofilm can consist of a single microbial species or a combination of different species of bacteria, protozoa, archaea, algae, filamentous fungi, and yeast that strongly attach to each other and to biotic or abiotic surfaces
bacterial biofilm formation relies on the interaction between the bacterial cells, the substrates and the surrounding media . And the formation of bacterial biofilms is a multi-step process starting with reversible attachment to surfaces aided by intermolecular forces and hydrophobicity, and then progress to extracellular polymeric substances (EPS) production which enable the cells to permanently adhere to a surface
there are five main phases involved in the biofilm formation process:
reversible attachment
irreversible attachment
EPS production
maturation of biofilm
dispersal/detachment
There are various methods to detect biofilm production like :
The microtiter plate (also called 96-well plate) assay
Tissue Culture Plate (TCP).
Tube method (TM).
Congo Red Agar method (CRA).
bioluminescent assay.
piezoelectric sensors.
fluorescent microscopic examination.
Biofilm
Biofilm is a group of microorganisms that form a slimy layer on surfaces. It begins with bacteria attaching to a surface through weak bonds and then excreting a protective matrix. As more bacteria accumulate, the biofilm develops into a complex structure. Biofilms can form in many environments and have properties that make the bacteria very resistant. They can be found in places like hot springs, rivers, medical implants, and pipelines. Biofilms impact many industries and can promote infections.
Clinical implications of bacterial biofilms
Biofilm formation has been implicated in persistent tissue infections such as chronic wound infection, chronic otitis media, chronic osteomyelitis, chronic rhinosinusitis, recurrent urinary tract infection, endocarditic and cystic fibrosis-associated lung infection.They are equally resistant to various antimicrobial treatments compared to their planktonic form
04 microbial biofilm_i_2008
This document provides an overview of microbial biofilms. It discusses how biofilms form and develop through attraction, adhesion, aggregation and accumulation of cells and extracellular matrix. The architecture and properties of biofilms are described as depending on nutrient levels and forming complex structures with channels. Biofilms provide benefits for microbes like cooperation, gene transfer and protection. They display higher resistance to toxic substances than planktonic microbes. The document traces the history of biofilm research and argues microbiology was previously misled by a focus on planktonic cultures rather than natural biofilm growth.
Biofilm
introduction of biofilm in microorganisms and stage of biofilm formation and method of detected biofilm-forming bacteria.
Getinge ATP Biofilm Course Booklet & Test
This document provides an overview of biofilm and bioburden contamination monitoring. It begins with an introduction to healthcare-associated infections (HAIs) and their costs. It then defines bioburden and describes how biofilm forms and behaves, including its resistance properties. Key areas where biofilm and bioburden commonly occur are medical devices like endoscopes. The document outlines testing methods for contamination, including protein and ATP bioluminescence tests. It concludes that contamination monitoring is important for improving patient safety and preventing HAIs.
Biophilms
Biofilm is a complex aggregate of microorganisms that attach to each other and to surfaces. Bacteria predominantly exist in biofilms, embedded in a self-produced matrix. Biofilm formation involves three phases - attachment, maturation, and dispersion. During attachment, bacteria attach reversibly and then irreversibly to surfaces. In maturation, bacteria activate genes to recruit other cells and build a matrix. Mature biofilms are resistant to antibiotics and host defenses. Dispersion spreads biofilms to new surfaces. Biofilms pose threats as the leading cause of healthcare-associated infections and are resistant to treatment.
Recommended
Microbial biofilms formation
There are many studies about bacterial and fungal biofilm which they were considered a very big problem now days ,because of that it was one of the most virulence factors which in turns increased resistant for antibiotics
Bacterial biofilms are complex surface attached communities of bacteria held together by self-produced polymer matrixs mainly composed of polysaccharides, secreted proteins, and extracellular DNAs
A biofilm can consist of a single microbial species or a combination of different species of bacteria, protozoa, archaea, algae, filamentous fungi, and yeast that strongly attach to each other and to biotic or abiotic surfaces
bacterial biofilm formation relies on the interaction between the bacterial cells, the substrates and the surrounding media . And the formation of bacterial biofilms is a multi-step process starting with reversible attachment to surfaces aided by intermolecular forces and hydrophobicity, and then progress to extracellular polymeric substances (EPS) production which enable the cells to permanently adhere to a surface.
there are five main phases involved in the biofilm formation process:
reversible attachment
irreversible attachment
EPS production
maturation of biofilm
dispersal/detachment
Microbial biofilms formation
Formation of microbial biofilms preparing by:
Assist. Lect. Aysar Ashour Khalaf
There are many studies about bacterial and fungal biofilm which they were considered a very big problem now days ,because of that it was one of the most virulence factors which in turns increased resistant for antibiotics . Bacterial biofilms are complex surface attached communities of bacteria held together by self-produced polymer matrixs mainly composed of polysaccharides, secreted proteins, and extracellular DNAs
Bacterial biofilms are complex surface attached communities of bacteria held together by self-produced polymer matrixs mainly composed of polysaccharides, secreted proteins, and extracellular DNAs
A biofilm can consist of a single microbial species or a combination of different species of bacteria, protozoa, archaea, algae, filamentous fungi, and yeast that strongly attach to each other and to biotic or abiotic surfaces
bacterial biofilm formation relies on the interaction between the bacterial cells, the substrates and the surrounding media . And the formation of bacterial biofilms is a multi-step process starting with reversible attachment to surfaces aided by intermolecular forces and hydrophobicity, and then progress to extracellular polymeric substances (EPS) production which enable the cells to permanently adhere to a surface
there are five main phases involved in the biofilm formation process:
reversible attachment
irreversible attachment
EPS production
maturation of biofilm
dispersal/detachment
There are various methods to detect biofilm production like :
The microtiter plate (also called 96-well plate) assay
Tissue Culture Plate (TCP).
Tube method (TM).
Congo Red Agar method (CRA).
bioluminescent assay.
piezoelectric sensors.
fluorescent microscopic examination.
Biofilm
Biofilm is a group of microorganisms that form a slimy layer on surfaces. It begins with bacteria attaching to a surface through weak bonds and then excreting a protective matrix. As more bacteria accumulate, the biofilm develops into a complex structure. Biofilms can form in many environments and have properties that make the bacteria very resistant. They can be found in places like hot springs, rivers, medical implants, and pipelines. Biofilms impact many industries and can promote infections.
Clinical implications of bacterial biofilms
Biofilm formation has been implicated in persistent tissue infections such as chronic wound infection, chronic otitis media, chronic osteomyelitis, chronic rhinosinusitis, recurrent urinary tract infection, endocarditic and cystic fibrosis-associated lung infection.They are equally resistant to various antimicrobial treatments compared to their planktonic form
04 microbial biofilm_i_2008
This document provides an overview of microbial biofilms. It discusses how biofilms form and develop through attraction, adhesion, aggregation and accumulation of cells and extracellular matrix. The architecture and properties of biofilms are described as depending on nutrient levels and forming complex structures with channels. Biofilms provide benefits for microbes like cooperation, gene transfer and protection. They display higher resistance to toxic substances than planktonic microbes. The document traces the history of biofilm research and argues microbiology was previously misled by a focus on planktonic cultures rather than natural biofilm growth.
Biofilm
introduction of biofilm in microorganisms and stage of biofilm formation and method of detected biofilm-forming bacteria.
Getinge ATP Biofilm Course Booklet & Test
This document provides an overview of biofilm and bioburden contamination monitoring. It begins with an introduction to healthcare-associated infections (HAIs) and their costs. It then defines bioburden and describes how biofilm forms and behaves, including its resistance properties. Key areas where biofilm and bioburden commonly occur are medical devices like endoscopes. The document outlines testing methods for contamination, including protein and ATP bioluminescence tests. It concludes that contamination monitoring is important for improving patient safety and preventing HAIs.
Biophilms
Biofilm is a complex aggregate of microorganisms that attach to each other and to surfaces. Bacteria predominantly exist in biofilms, embedded in a self-produced matrix. Biofilm formation involves three phases - attachment, maturation, and dispersion. During attachment, bacteria attach reversibly and then irreversibly to surfaces. In maturation, bacteria activate genes to recruit other cells and build a matrix. Mature biofilms are resistant to antibiotics and host defenses. Dispersion spreads biofilms to new surfaces. Biofilms pose threats as the leading cause of healthcare-associated infections and are resistant to treatment.
Prasanth02
Biofilm formation involves the accumulation of microorganisms on surfaces where they form sessile communities encased in an extracellular polymeric substance matrix. Biofilm formation progresses through initial attachment, irreversible attachment aided by EPS production, early development, maturation, and dispersion. Virulence factors and quorum sensing play important roles in biofilm formation. Mature biofilms pose challenges for antibiotic treatment and are implicated in various pathogenic conditions like cystic fibrosis, periodontitis, and device-related infections. Therapeutic strategies aim to target biofilm formation, virulence factors, and quorum sensing.
Biofilm
This document discusses wound biofilms and their impact on wound healing. It begins by defining biofilms and planktonic cells. Biofilms are bacterial communities enclosed in an extracellular matrix that are adherent to surfaces. They are resistant to antibiotics and host immune responses. The presence of biofilms in wounds can trigger chronic inflammation and inhibit wound healing by damaging tissue through reactive oxygen species. There are no definitive diagnostic tests for biofilms but tissue biopsies may reveal them. Treatment involves thorough debridement combined with antibiofilm agents like lactoferrin or quorum sensing inhibitors, along with preventing their formation in at-risk wounds through proper care.
05 microbial biofilm_ii_2008
This document summarizes key properties and challenges of microbial biofilms. It discusses how biofilms form complex structures that protect microbes and enable gene transfer. Antibiotics have difficulty penetrating biofilms and the local environment inside biofilms can alter antibiotic effectiveness. Biofilms also harbor subpopulations of highly resistant cells. The document outlines how biofilms contribute to various chronic infections and problems in fields like engineering. It reviews current and potential future strategies for disrupting or preventing biofilms.
Biofilms
Biofilms allow bacteria to survive in hostile environments by forming protective extracellular matrices. Bacteria in biofilms are up to 1,000 times more resistant to antibiotics and disinfectants than individual planktonic bacteria. Key factors in biofilm formation include bacterial attachment, production of extracellular polymeric substances, and cell-cell communication via quorum sensing. Biofilms play an important role in many infectious diseases by protecting bacteria and allowing resistance to host immune responses and antibiotic treatment.
Biofilms in chronic suppurative otitis media and cholesteatoma
This document discusses biofilms and their role in chronic otorhinolaryngologic infections. It describes the 5 stages of biofilm development and explains how biofilms differ from planktonic bacteria. Biofilms form complex structures that are resistant to antibiotics due to reduced metabolic activity, stress response activation, collective neutralization of antimicrobials, and accumulation of persister cells. Scanning electron microscopy is used to visualize biofilms and reveal their fine structures. Biofilms are implicated in a variety of chronic infections like chronic suppurative otitis media, cholesteatoma, implanted medical devices, and rhinosinusitis. Effective treatment strategies include interrupting quorum sensing and biofilm matrix disruption.
Biofilms
This document provides information about biofilms and their role in implant-associated infections. It discusses how biofilms form in four steps and how bacteria in biofilms differ from planktonic bacteria through increased cooperation and resistance to antimicrobial agents. Treatment of implant infections is challenging as antibiotics are often ineffective against biofilms. The document outlines approaches to treatment, including combination antibiotics, surgical debridement and implant removal, as well as newer modalities like local antibiotic delivery and quorum sensing inhibitors. However, it notes that implant infections often require complete removal of the infected device for elimination.
Biofilms in otorhinolaryngology
This document discusses bacterial biofilms, including their structure, formation through quorum sensing, role in chronic and recurrent infections, and diagnosis using techniques like confocal laser microscopy. Biofilms play an important role in many diseases like chronic rhinosinusitis, otitis media, and implant infections by protecting bacteria from antibiotics and host defenses. Their presence is associated with worse treatment outcomes. Diagnosis of biofilm infections still relies on direct microscopy visualization of the biofilm matrix and embedded microbes.
Intro To Biofilms
This document provides an overview of biofilms and their relevance to pharmaceutical manufacturing. It discusses how biofilms can cause both opportunities and problems in the industry. Biofilms are defined as the unwanted adhesion of microorganisms to surfaces, which can lead to manufacturing issues like product contamination, process downtime, and sampling difficulties. The document outlines the typical sequence of biofilm formation and highlights their increased resistance to antimicrobials and cleaning agents. It also introduces hurdle technology as an approach to prevent biofilms through multiple barriers in the manufacturing process.
Biofilm
This document discusses dental biofilms, also known as dental plaque. It explains that dental biofilms are three-dimensional, multispecies microbial communities that form on teeth and other oral surfaces. The key points covered include:
- Dental biofilms provide benefits to microorganisms like increased habitat range and stress tolerance.
- They form through the adsorption of a conditioning film, followed by reversible and then permanent bacterial attachment and colonization.
- As biofilms mature, they develop complex architecture, metabolic gradients, cell signaling pathways, and interspecies interactions between diverse microbes.
- While associated with diseases like caries and periodontitis, the oral microbiome also benefits the host through commensalism
Biofilms IN ENT
This document summarizes biofilms and their role in ENT diseases. It discusses the formation and development of biofilms, mechanisms of antibiotic resistance, and how biofilms are involved in diseases like chronic rhinosinusitis, otitis media, tonsillitis and infected implants. Detection methods like SEM, FISH and CLSM are described. Treatment focuses on physically removing biofilms, using macrolide antibiotics or disrupting quorum sensing, though developing effective anti-biofilm treatments remains a challenge.
BIOFILM FORMATION
this ppt deals with biofilm formation mechanism y microbes produce it and how it affects humans in a brief manner
Fungal biofilm
This document summarizes key information about fungal biofilms. It discusses how biofilms are formed through distinct developmental phases. Biofilms are composed of microbial cells embedded in an extracellular matrix that provides structure and protects cells. Many medically important fungi like Candida, Aspergillus, and Cryptococcus can form biofilms. Biofilms contribute to infections associated with medical devices and are highly resistant to antifungal treatments. The formation and structure of fungal biofilms is regulated by complex genetic and environmental factors.
Biofilms
Bacterial biofilms are communities of surface-associated microorganisms encased in a self-produced extracellular matrix. Biofilm formation is a nearly universal bacterial trait found on natural and artificial surfaces. The document discusses the complex structure of biofilms and the different environmental niches within biofilms that allow cells to experience different conditions. It also examines the stages of biofilm formation and dispersion, and how biofilms can lead to drug resistance and infectious diseases in humans.
Biofilm
The document discusses biofilms and their antibiotic resistance. It notes that biofilms are assemblages of surface-attached microbial cells encased in an extracellular matrix. This matrix provides protection from antibiotics by acting as a diffusion barrier and binding antimicrobial agents. Additionally, the heterogeneous conditions within biofilms, including nutrient depletion and slower growth, contribute to antibiotic tolerance in some cells. Genetic transfer of resistance genes is also facilitated within dense biofilm communities.
Probiotics and biofilms
This document provides an overview of probiotics and biofilms. It begins with definitions of probiotics, prebiotics, and synbiotics. The mechanisms of action of probiotics are then described, including anti-cancer, anti-diarrheal, immunomodulation, and anti-allergy effects. Biofilm formation, development, and dispersal are explained. Key properties of biofilms like their structure, quorum sensing, and antibiotic resistance are covered. The roles of biofilms in infectious diseases and examples like dental caries are highlighted. Control and removal of biofilms is also discussed.
Microbial biofilm
This document provides information about bacterial biofilms. It discusses what biofilms are, examples of biofilms, how they form, their properties, composition, impacts and problems they can cause. It also covers antibiotic resistance in biofilms and strategies to control biofilms, including using quorum sensing inhibitors, improving drug delivery systems, and preventing biofilm formation and colonization.
Micro chapter 31 biofilms - architects of disease
Biofilms are communities of organisms attached to a solid surface that can consist of multiple species living and interacting together. They evolve over time and are important as they represent cooperative multi-organism populations. Biofilms exist in two main types - sessile biofilms that are permanently anchored to a surface, and planktonic biofilms that are free-floating and can move to new habitats. Examples of biofilms include those found in water pipes, airplane ventilation systems, wine casks, and the lungs of cystic fibrosis patients.
Biofilms- Dr. Richa Maurya
- Bacteria frequently form biofilms, which are groups of microorganisms that attach to surfaces and produce an extracellular matrix. Over 99% of bacteria exist in biofilms.
- Biofilms provide bacteria protection from environmental threats like antibiotics. The extracellular matrix restricts antibiotic penetration and biofilms can be up to 1000 times more resistant to antibiotics than planktonic bacteria.
- Biofilm formation involves an initial attachment phase, followed by growth of colonies on the surface and eventual detachment of planktonic cells to form new biofilms elsewhere.
Biofilm information in pathogen bacteria
This document provides an overview of biofilm formation in pathogens bacteria. It defines biofilms and describes their composition and structure. Biofilms provide bacteria advantages like increased antibiotic resistance. The document discusses where biofilms are commonly found and their role in various infectious diseases. It also reviews several studies examining biofilm formation in specific pathogens like Pseudomonas aeruginosa, Staphylococcus aureus, and Mycobacterium avium.
Biofilms by krishanchand mahra
Microorganisms like bacteria, fungi and protists can form biofilms, which are colonies that grow attached to surfaces. Biofilms have a complex structure with layers of polysaccharides and channels that allow for nutrient transport and waste removal. They form through initial attachment to a surface, irreversible attachment, microcolony formation, maturation, and eventual detachment of cells. Quorum sensing allows biofilm bacteria to communicate and coordinate behaviors. While biofilms can be used for bioremediation and wastewater treatment, they also pose disadvantages as they are implicated in infections, environmental impacts, industrial issues, and device infections.
Formation Structure and Internal Functions of Microbial mats And Biofilms
This document provides an overview of microbial mats and biofilms. It defines both as surface-associated layers of microbial cells embedded in an extracellular matrix. Microbial mats are multilayer sheets that cover sediments and are composed primarily of bacteria and archaea. Microbial mats were some of the earliest life forms on Earth, dating back 3.5 billion years ago. They form complex communities that allow bacteria to survive in harsh environments. Biofilms are microbial communities that attach to and cover solid surfaces. They develop through a multi-stage process and contain diverse microorganisms, including bacteria, archaea, protozoa, fungi and algae. Both microbial mats and biofilms are important in environmental engineering applications like wastew
BIOFILMS.pptx
The document discusses aerobic biofilm processes and the formation and characteristics of biofilms. It explains that biofilms are assemblages of microbial cells that attach to surfaces and each other, encased in a self-produced matrix. Biofilm formation involves initial attachment of cells, proliferation, and eventual dispersion. Biofilms are ubiquitous in nature and important for processes like bioremediation and wastewater treatment. They create unique microenvironments and cells can communicate within biofilms.
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Prasanth02
Biofilm formation involves the accumulation of microorganisms on surfaces where they form sessile communities encased in an extracellular polymeric substance matrix. Biofilm formation progresses through initial attachment, irreversible attachment aided by EPS production, early development, maturation, and dispersion. Virulence factors and quorum sensing play important roles in biofilm formation. Mature biofilms pose challenges for antibiotic treatment and are implicated in various pathogenic conditions like cystic fibrosis, periodontitis, and device-related infections. Therapeutic strategies aim to target biofilm formation, virulence factors, and quorum sensing.
Biofilm
This document discusses wound biofilms and their impact on wound healing. It begins by defining biofilms and planktonic cells. Biofilms are bacterial communities enclosed in an extracellular matrix that are adherent to surfaces. They are resistant to antibiotics and host immune responses. The presence of biofilms in wounds can trigger chronic inflammation and inhibit wound healing by damaging tissue through reactive oxygen species. There are no definitive diagnostic tests for biofilms but tissue biopsies may reveal them. Treatment involves thorough debridement combined with antibiofilm agents like lactoferrin or quorum sensing inhibitors, along with preventing their formation in at-risk wounds through proper care.
05 microbial biofilm_ii_2008
This document summarizes key properties and challenges of microbial biofilms. It discusses how biofilms form complex structures that protect microbes and enable gene transfer. Antibiotics have difficulty penetrating biofilms and the local environment inside biofilms can alter antibiotic effectiveness. Biofilms also harbor subpopulations of highly resistant cells. The document outlines how biofilms contribute to various chronic infections and problems in fields like engineering. It reviews current and potential future strategies for disrupting or preventing biofilms.
Biofilms
Biofilms allow bacteria to survive in hostile environments by forming protective extracellular matrices. Bacteria in biofilms are up to 1,000 times more resistant to antibiotics and disinfectants than individual planktonic bacteria. Key factors in biofilm formation include bacterial attachment, production of extracellular polymeric substances, and cell-cell communication via quorum sensing. Biofilms play an important role in many infectious diseases by protecting bacteria and allowing resistance to host immune responses and antibiotic treatment.
Biofilms in chronic suppurative otitis media and cholesteatoma
This document discusses biofilms and their role in chronic otorhinolaryngologic infections. It describes the 5 stages of biofilm development and explains how biofilms differ from planktonic bacteria. Biofilms form complex structures that are resistant to antibiotics due to reduced metabolic activity, stress response activation, collective neutralization of antimicrobials, and accumulation of persister cells. Scanning electron microscopy is used to visualize biofilms and reveal their fine structures. Biofilms are implicated in a variety of chronic infections like chronic suppurative otitis media, cholesteatoma, implanted medical devices, and rhinosinusitis. Effective treatment strategies include interrupting quorum sensing and biofilm matrix disruption.
Biofilms
This document provides information about biofilms and their role in implant-associated infections. It discusses how biofilms form in four steps and how bacteria in biofilms differ from planktonic bacteria through increased cooperation and resistance to antimicrobial agents. Treatment of implant infections is challenging as antibiotics are often ineffective against biofilms. The document outlines approaches to treatment, including combination antibiotics, surgical debridement and implant removal, as well as newer modalities like local antibiotic delivery and quorum sensing inhibitors. However, it notes that implant infections often require complete removal of the infected device for elimination.
Biofilms in otorhinolaryngology
This document discusses bacterial biofilms, including their structure, formation through quorum sensing, role in chronic and recurrent infections, and diagnosis using techniques like confocal laser microscopy. Biofilms play an important role in many diseases like chronic rhinosinusitis, otitis media, and implant infections by protecting bacteria from antibiotics and host defenses. Their presence is associated with worse treatment outcomes. Diagnosis of biofilm infections still relies on direct microscopy visualization of the biofilm matrix and embedded microbes.
Intro To Biofilms
This document provides an overview of biofilms and their relevance to pharmaceutical manufacturing. It discusses how biofilms can cause both opportunities and problems in the industry. Biofilms are defined as the unwanted adhesion of microorganisms to surfaces, which can lead to manufacturing issues like product contamination, process downtime, and sampling difficulties. The document outlines the typical sequence of biofilm formation and highlights their increased resistance to antimicrobials and cleaning agents. It also introduces hurdle technology as an approach to prevent biofilms through multiple barriers in the manufacturing process.
Biofilm
This document discusses dental biofilms, also known as dental plaque. It explains that dental biofilms are three-dimensional, multispecies microbial communities that form on teeth and other oral surfaces. The key points covered include:
- Dental biofilms provide benefits to microorganisms like increased habitat range and stress tolerance.
- They form through the adsorption of a conditioning film, followed by reversible and then permanent bacterial attachment and colonization.
- As biofilms mature, they develop complex architecture, metabolic gradients, cell signaling pathways, and interspecies interactions between diverse microbes.
- While associated with diseases like caries and periodontitis, the oral microbiome also benefits the host through commensalism
Biofilms IN ENT
This document summarizes biofilms and their role in ENT diseases. It discusses the formation and development of biofilms, mechanisms of antibiotic resistance, and how biofilms are involved in diseases like chronic rhinosinusitis, otitis media, tonsillitis and infected implants. Detection methods like SEM, FISH and CLSM are described. Treatment focuses on physically removing biofilms, using macrolide antibiotics or disrupting quorum sensing, though developing effective anti-biofilm treatments remains a challenge.
BIOFILM FORMATION
this ppt deals with biofilm formation mechanism y microbes produce it and how it affects humans in a brief manner
Fungal biofilm
This document summarizes key information about fungal biofilms. It discusses how biofilms are formed through distinct developmental phases. Biofilms are composed of microbial cells embedded in an extracellular matrix that provides structure and protects cells. Many medically important fungi like Candida, Aspergillus, and Cryptococcus can form biofilms. Biofilms contribute to infections associated with medical devices and are highly resistant to antifungal treatments. The formation and structure of fungal biofilms is regulated by complex genetic and environmental factors.
Biofilms
Bacterial biofilms are communities of surface-associated microorganisms encased in a self-produced extracellular matrix. Biofilm formation is a nearly universal bacterial trait found on natural and artificial surfaces. The document discusses the complex structure of biofilms and the different environmental niches within biofilms that allow cells to experience different conditions. It also examines the stages of biofilm formation and dispersion, and how biofilms can lead to drug resistance and infectious diseases in humans.
Biofilm
The document discusses biofilms and their antibiotic resistance. It notes that biofilms are assemblages of surface-attached microbial cells encased in an extracellular matrix. This matrix provides protection from antibiotics by acting as a diffusion barrier and binding antimicrobial agents. Additionally, the heterogeneous conditions within biofilms, including nutrient depletion and slower growth, contribute to antibiotic tolerance in some cells. Genetic transfer of resistance genes is also facilitated within dense biofilm communities.
Probiotics and biofilms
This document provides an overview of probiotics and biofilms. It begins with definitions of probiotics, prebiotics, and synbiotics. The mechanisms of action of probiotics are then described, including anti-cancer, anti-diarrheal, immunomodulation, and anti-allergy effects. Biofilm formation, development, and dispersal are explained. Key properties of biofilms like their structure, quorum sensing, and antibiotic resistance are covered. The roles of biofilms in infectious diseases and examples like dental caries are highlighted. Control and removal of biofilms is also discussed.
Microbial biofilm
This document provides information about bacterial biofilms. It discusses what biofilms are, examples of biofilms, how they form, their properties, composition, impacts and problems they can cause. It also covers antibiotic resistance in biofilms and strategies to control biofilms, including using quorum sensing inhibitors, improving drug delivery systems, and preventing biofilm formation and colonization.
Micro chapter 31 biofilms - architects of disease
Biofilms are communities of organisms attached to a solid surface that can consist of multiple species living and interacting together. They evolve over time and are important as they represent cooperative multi-organism populations. Biofilms exist in two main types - sessile biofilms that are permanently anchored to a surface, and planktonic biofilms that are free-floating and can move to new habitats. Examples of biofilms include those found in water pipes, airplane ventilation systems, wine casks, and the lungs of cystic fibrosis patients.
Biofilms- Dr. Richa Maurya
- Bacteria frequently form biofilms, which are groups of microorganisms that attach to surfaces and produce an extracellular matrix. Over 99% of bacteria exist in biofilms.
- Biofilms provide bacteria protection from environmental threats like antibiotics. The extracellular matrix restricts antibiotic penetration and biofilms can be up to 1000 times more resistant to antibiotics than planktonic bacteria.
- Biofilm formation involves an initial attachment phase, followed by growth of colonies on the surface and eventual detachment of planktonic cells to form new biofilms elsewhere.
Biofilm information in pathogen bacteria
This document provides an overview of biofilm formation in pathogens bacteria. It defines biofilms and describes their composition and structure. Biofilms provide bacteria advantages like increased antibiotic resistance. The document discusses where biofilms are commonly found and their role in various infectious diseases. It also reviews several studies examining biofilm formation in specific pathogens like Pseudomonas aeruginosa, Staphylococcus aureus, and Mycobacterium avium.
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Biofilms in chronic suppurative otitis media and cholesteatoma
Biofilms in chronic suppurative otitis media and cholesteatoma
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Microorganisms like bacteria, fungi and protists can form biofilms, which are colonies that grow attached to surfaces. Biofilms have a complex structure with layers of polysaccharides and channels that allow for nutrient transport and waste removal. They form through initial attachment to a surface, irreversible attachment, microcolony formation, maturation, and eventual detachment of cells. Quorum sensing allows biofilm bacteria to communicate and coordinate behaviors. While biofilms can be used for bioremediation and wastewater treatment, they also pose disadvantages as they are implicated in infections, environmental impacts, industrial issues, and device infections.
Formation Structure and Internal Functions of Microbial mats And Biofilms
This document provides an overview of microbial mats and biofilms. It defines both as surface-associated layers of microbial cells embedded in an extracellular matrix. Microbial mats are multilayer sheets that cover sediments and are composed primarily of bacteria and archaea. Microbial mats were some of the earliest life forms on Earth, dating back 3.5 billion years ago. They form complex communities that allow bacteria to survive in harsh environments. Biofilms are microbial communities that attach to and cover solid surfaces. They develop through a multi-stage process and contain diverse microorganisms, including bacteria, archaea, protozoa, fungi and algae. Both microbial mats and biofilms are important in environmental engineering applications like wastew
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Biofilms
- Biofilms are groups of microorganisms that attach to each other and often surfaces, and are found nearly everywhere in nature. They can include bacteria, fungi, and protists. Common examples are dental plaque and pond scum.
- Biofilm formation is a complex, multi-step process involving initial attachment, microcolony formation within an extracellular matrix, maturation, and eventual dispersal or detachment of cells. Dispersal allows biofilms to spread and colonize new surfaces.
- Biofilms grow in many habitats and can cause problems like infections, corrosion, and difficulties with sanitation. They are implicated in about 80% of all infections and can involve both gram-positive and gram-negative bacteria as
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Biofilms
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URBAS ASHIQ presentation on bacterial biofilms
- 1. Presented By : Urbas Ashiq MSc Microbiology
- 2. 1) BACTERIAL BIOFILM 2) FORMATION... 3) SIGNIFICANCES and IMPACTS OF BACTERIAL BIOFILMS....
- 3. Biofilm is a well assembled microbial community of bacteria in general along with other microbes like fungi algae protozoa
- 4. Bacterial Bio film is made up of bacteria and their extracellular polymeric substances by which they attach to biotic or a biotic surfaces...
- 6. 1) A BACTERIAL BIO FILM IS A SURFACE ATTACHED COMMUNITY OF BACTERIAL CELLS. 2) FORMS IN AN AQUEOUS ENVIRONMENT, INCLUDING RIVERS,OCEANS,DRAINS....
- 7. 3) Involves a series of bacterial processes and begins as bacteria Seattle up on any surface and multiply
- 8. 4) Requires many bacteria, and may involve different types of bacteria working together... 5) Bacteria are equipped with a molecular signalling system, used in a process called “QUORM SENSING”.
- 9. 6)These signals triggers changes in Gean regulation 7)This causes reorganisation of cellular machinery for biofilm formation purpose...
- 10. 8) Now Bacteria produces thin extracellular fibres called “Pilli’”. Used for attachment to the substrate and to the each other. Pilli are also used to adjust their position , in a process called “twiitching motility”.
- 11. 9) NOW BACTERIA SECRET A SLIMY EXTRACELLULAR MATRIX OF PROTEINS,POLYSACCHIRIDES AND NUCLEIC ACIDS...
- 13. 12) These cells may produce flagella and swim to more favourable areas ,where they will form a new Biofilm
- 15. PROBLEMATIC IN SEVERAL FOOD INDUSTRIES DUE TO THE ABILITY TO FORM ON PLANTS DURING INDUSTRIAL PROCESSES. Bio films can also be harnessed for constructive purposes.
- 16. Bio films can absorb a variety of toxic substances including metal cations, toxins and antibiotics and protect residing bacteria from external environment....
- 17. Protects residing Bacteria from external environment such as UV radiation, pH,osmotic shock etc...
- 18. Protects residing Bacteria from external environment such as UV radiation, pH,osmotic shock etc...
- 19. Their presence in water pipelines, food processing units are also a subject to worry...
- 20. Moreover their formation leads to development of different species which are muti-drug resistant and is a cause of grate havoc to available treatment....
- 21. Thus this is very essential to stop their formulation at first stage by use of proper and clean material as well as effective sanitization and treatment...
- 22. Thank You