Kit Development using quantum computer and Genetic Engineering.

1. Development of Nucleic Acid Extraction Kit:
Employing Quantum Computing and Genetic
Engineering Approach
Preseneted by:
Kazy Ebnul Hasan
Nibedita Deb, Nazmir-Nur Showva, Tahmina
Afroze Bondhon, Md Sakibul Islam,
Dr. Akhtaruzzaman Chowdhury
BANGLADESH REFERENCE INSTITUTE for CHEMICAL MEASUREMENTS
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2. Robotics
AI
Nano-
technology
Quantum
Computing
IoT
Genetic
Engineering
4th
Industrial
Revolution
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3. 1. Nucleic Acid Extraction
from micro-organism
2. Production of proteinase k
enzyme through
genetically modified
microorganism.
1. Time Saving
Approach
2. Cost Effective.
Quantum Computing and Genetic Engineering
1. Qubit Orbital Mapping to
simplify the problem
2. Considering Gate Error
3. VQE, Trotterization for
Excited state
4. Run the circuit with
iteration 3

4. Nucleic Acid Extraction Kit
• Molecular Diagnosis Purpose
Covid-19 Detection, HIV, Cancer
• Research purpose
Academia, Industrial
• Available Kit in Bangladesh and Market Price:
Bioland Scientific LLC – 180 USD
Mag-Bind Viral RNA extraction kit - 410 USD
GeneProof RNA extraction Kit- 200 USD
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5. Project Purpose
Nucleic Acid
Extraction Kit
SDG Goal 9
Cost
Effective
Reduce Import
Dependency
Skilled
Manpower
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6. Project Objectives
Development of Nucleic Acid Extraction Kit
Development of Standard Protocol by Quantum
Computing
Quantification of Nucleic Acid
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7. Method
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8. Alkaline Extraction Method
• Glucose, Tris-Cl, EDTA
• Lysis solution (NaOH, SDS)
Resuspension Buffer
• Na-acetate
• Glacial Acetic Acid
•Neutralization Solution
• 75% Ethanol
•Wash Buffer
• DNAase free Water
• TE Buffer (Tris-Cl, EDTA)
Elution Buffer
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9. Silica gel membrane Method
• SDS
• 0.5% (w/v) SDS, 0.05 M Tris⋅Cl, Adjust pH to
8.0, Add 1 mM fresh dithiothreitol (DTT)
Lysis Buffer
• NaCl, 𝑀𝑔𝐶𝑙2 (High Salt Concentration)
• Ethanol/ Isopropanol
Binding Buffer
• 80% Ethanol
Wash Buffer
• Low salt water
Elution Buffer
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10. Sample+
Lysis
Buffer +
Proteinas
ek
enzyme
Add Wash
Buffer 1
Centrifuge
Add Wash Buffer 2
Add Elution
Buffer
RNA/
DNA
Lysis Buffer
Wash 1
Wash 2
Elution
Buffer
Proteinase k
enzyme
Extraction Process
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11. Application
• Specimens Identification
• Obtaining Biological Information
• Personalized Medicine & Pharmacogenomics
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12. Conclusion
• Nucleic acid extraction plays a vital role in molecular biology as the primary
step form any downstream applications.
• No general gold-standard method has yet been established.
• Despite complexity and long time simulation, Quantum Computing could be
viable for precise calculation which may lead to a standard Nucleic Acid
Extraction protocol.
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13. Thank You
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