Types of PCR (Polymerase Chain Reaction) Nested & Multiplex PCR with Difference

1. DISCOVER . LEARN . EMPOWER
Nested PCR and Multiplex PCR
UNIVERSITY INSTITUTE OF
BIOTECHNOLOGY
Master of Sciences (Biotechnology)
Subject Name: Genetic Engineering
Code : 24BTT612

2. Nested PCR
Two pairs (instead of one pair) of PCR
primers are used to amplify a fragment.
• First pair -amplify a fragment similar to a
standard PCR. Second pair of primers-
nested primers (as they lie / are nested
within the first fragment) bind inside the
first PCR product fragment to allow
amplification of a second PCR product
which is shorter than the first one.
• Advantage- Very low probability of
nonspecific amplification

3. Multiplex PCR
• Multiplex PCR is a variant of PCR which enabling
simultaneous amplification of many targets of interest
in one reaction by using more than one pair of primers.

4. UIBT, Chandigarh University
Dr. Gajendra B. Singh 4
OUTCOME
• After the completion of lecture students will gain in-depth knowledge on
PCR, its modified forms and their applications in genetic engineering.
• Students will also be able to apply the knowledge in problem solving and in
practice.

5. UIBT, Chandigarh University
Dr. Gajendra B. Singh 5
BOOKS
Text Books
Sr No Title Author Edition Year Publisher Name
1
An Introduction to Genetic
Engineering Desmond S. T. Nicholl III 2008 Cambridge University Press
2
Gene Cloning and DNAAnalysis:
An Introduction T.A. Brown VII 2016 Wiley-Blackwell
3
Techniques in Genetic
Engineering Isil Aksan Kurnaz I 2015 CRC Press
Reference Books
Sr.No. Title Author Edition Year Publisher Name
1
Principles of Gene Manipulation
and Genomics
S. B. Primrose and R. M.
Twayman
VII 2006 Wilky-Blackwell
2
Genetic Engineering of
Horticultural Crops
Gyana Ranjan Rout, K.V.
Peter I 2018 Academic Press

6. THANK YOU
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