Molecular detection of HPV and FT-IR spectroscopy analysis in women with normal cervical cytology.

1. DIEGO ALEJANDRO BERRIO USUGA
TERCER SEMESTRE 2020-1
Facultad de Medicina – Universidad Pontificia Bolivariana

2. INTRODUCTION
• they belong to the family of Papillomaviridae.
• It is one of the most common sexually transmitted
diseases. HPVs are viruses that replicate specifically
in the nucleus of squamous epithelial cells.
• They are very similar, therefore HPVs cannot be
classified into serotypes. Its classification in
genotypes and subtypes is based on the differences
in its DNA sequence.

3. INTRODUCTION
• 100 viral types.
• They are usually asymptomatic, only some
cause warts or condylomas and a smaller
proportion cause cervical, vulvar, vagina and
anus cancer in women, or anal and penile
cancer in men.
THEY ARE CLASSIFIED INTO HIGH AND
LOW CANCER RISK TYPES.
HPV types 16, 18, 31,
33, 35, 39, 45, 51,
52, 56, 58, 59 and 66
are carcinogenic to
humans - types of
high cancer risk.
HPV 6 and HPV 11 are
possible carcinogens
for humans - types of
low cancer risk.

4. •This study aimed to demonstrate the
effectiveness and sensitivity of molecular
analysis by PCR in relation to cytological
analysis and to evaluate the sensitivity of
FT-IR spectroscopy in the diagnosis of HPV
for cervical cancer prevention.

5. • CITOLOGÍA (prueba de Papanicolaou)
• FUNDAMENTO: Es un método de tinción policrómatico que consta de una
tinción nuclear y un contraste citoplasmático.

6. • FUNDAMENTO:
• es una técnica en la cual se
amplifica una secuencia de
material genético para
identificar la presencia de
una mutación.
• ¿PARA QUE?: para la
amplificación del gen viral
L1.

7. • FUNDAMENTO:
• Es el estudio del espectro
luminoso de los cuerpos para
poder determinar la composición
cualitativa y cuantitativa de una
muestra. Permite detectar la
absorción o emisión de radiación
electromagnética de ciertas
energías y relacionarlas con
otros tipos de energía que sirvan
de muestra.
¿PARA QUE?
VPH + VPH-
7 MUESTRAS
+
6 MUESTRAS
(CONTROL) +
Amplificación
gen L1 (PCR)
43
MUESTRAS -

8. • FUNDAMENTO:
• Técnica de medición
que permite registrar
espectros infrarrojos a
través de un
interferómetro y luego
a través de la muestra
(o viceversa).
• ¿PARA QUE?: Para clasificar los datos
normales y grupos de VPH, se analizan a
modo de contraste mediante análisis
discriminante según la clasificación de PCR.

12. AUTHOR CONCEPT COMMENT
Rimsza etal The Papanicolaou test is the most
used primary care (SUS) test and is
established by the Brazil Government
Health Service to be applied in
patients from the age of 25 for the
early detection of cervical cancer,
which occurs most commonly in
women above 30 years.
I think that this test is the best in
cost-benefit, but that it is not a very
accurate test. But with the monetary
deficiency of the ministries of health
in the different countries, we will
hardly be able to have the PCR
performed as a primary care test.
A. Adenis, V. Dufit. Studies have shown that the
prevalence of this infection is
significantly higher in the younger
female population.
I agree with this assessment, but I
think they should still do a study to
find out why this happens.
H.C. Kitchener, E.P. Silva. With the application of molecular
biology techniques, it is possible to
identify the HPV DNA in 99.7% of the
types of invasive cervical cancer.
This is totally true, the idea is that a
future will apply molecular biology
techniques, not only to HPV, but also
to all pathologies. Molecular biology
is the medicine of the future.

13. The PCR technique is the most effective technique to diagnose HPV
prematurely, a greater effort must be made so that in the future,
the PCR is a rapid and low-cost test.
FT-IR spectroscopy is not a diagnostic test, despite providing useful
data, in contrast to PCR that is 97% reliable.

14. MAPA CONCEPTUAL

15. GRACIAS