This document outlines a proposed breeding plan to introgress resistance traits from wild peanut species into cultivated peanut varieties. The plan involves first creating a synthetic amphidiploid from a cross between wild species and cultivated peanut. This synthetic would then be backcrossed multiple times to the cultivated variety Florunner while performing marker-assisted selection to track wild introgressions. After 7-10 seasons, lines with introgressed resistance traits would be selected and released as new peanut varieties. The goal is to expand the genetic diversity and introduce biotic and abiotic stress resistance traits that are currently lacking in cultivated peanut germplasm.

1. Breeding for Resistance
to Biotic Stresses: An
Introgression Pathway in
Groundnut
Sameer Khanal
University of Georgia

2. Peanut Breeding Goals, Priorities, and
Constraints
Goals and Priorities:
 Yield
 Early Maturity
 Resistance to Biotic/Abiotic Stresses
 Seed Quality Traits: Improved Oil Quality and Flavor, Aspergillus resistance
Constraints:
 Lack of adequate allelic diversities among cultivated accessions
 Difficult to introgress traits of interest from diploid wild spp.

3. Cultivar Development
A. cardinasii (AA) A. diogoi (AA)X
AA
TXAG-6
Parents
Interspecific hybridization
3-way hybrid
Chromosome doubling
Synthetic
Amphidiploid
X
Florunner
AB
Interspecific hybridization
X
A. batizocoi (BB)
BC5F3
COAN1
Development of COAN and NemaTAM
NemaTAM2
BC7F3
1. Simpson and Starr 2. Simpson et al. 2003

4. Proposed Plan
F1
Modern Cultivar
AABB (2n = 4x =40)
Colchicine Double
Synthethic Allotetraploid
AABB (2n = 4x =40)
Marker-Assisted Selection
A/B-Genome
Wild Introgression Lines
AABB (2n = 4x =40)
A. duranensis A. batizocoi

5. Background for the Proposed Plan
1. More than 2,000 transcriptome-based and couple hundred genomic-based
SSR markers are available
2. A large number of SNPs are identified
3. Saturated A- and B-genome diploid linkage maps are on the pipeline
4. Comparative mapping and synteny analysis with model genomes will facilitate
gene tagging
5. Traits of interest will be mapped along the chromosomes

6. Outline of the Breeding Scheme
1. First Season: Florunner x Synthetic Amphidiploid
2. Second Season: Growing F1 and Backcrossing to Florunner
3. Third Season: BC1F1 Genotyped and Backcrossed to Florunner
4. Fourth – Fifth Season: Repeated Backcrosses
5. Sixth Season: BC3F1 Genotyped and Selfed
6. Seventh-Eighth Season: Towards introgression library (Selfed)
7. Ninth-Tenth Season: Multilocational trials/Marker-trait Association
8. Seed Increase of Select Lines and Varietal Release.
9. Minimum of Seven Years (2 seasons per year) from Initial Crossing to
Ultimate Release of a Variety.

7. Thank you
Acknowledgement:
PBGG