Presentation on "Determination of packed cell volume" is performed by first Prof. D.V.M. in the Department of Physiology & Biochemistry, Sindh Agriculture University Tandojam Pakistan.

1. ِِ‫م‬ْ‫س‬ِ‫ب‬
ِ
ِ ّٰ
‫ٱَلل‬
ِِ‫ن‬ ٰ‫م‬ْ‫ح‬ّ‫ٱلر‬
ِّ‫ٱلر‬
‫مم‬ ِ‫ح‬
ِ
In the Name of Allah, the Most
Beneficent, the Most Merciful.

2. Assignment
Subject: PHY-101
Topic: Determination Of PCV (Packed Cell Volume)
Presented By : Muqadas Soomro, Moazza Khalid
Lareb qaimkhani, Murk bhutto, Rukhsana Ghazi, Nimra Rajput, Muhammad
Kashif, Muhammad Bilal, Muhammad Jamshed, Muntazar Hussain,
Muhammad Aamir, Muti-ur-rehman Saad, Yagaana Ali, Muhammad Zain,
pawan Kumar, Muhammad Asim, Kazim Raza, Muhammad Talha, Mohsin and
Pirbhat bheel

3. SUBMITTED TO
Dr. ALLAH BUX KACHIWAL
PROFESSOR , CHAIRMAN
DEPARTMENT OF PHYSIOLOGY AND BIOCHEMISTRY

4. Introduction :
• The packed cell volume (PCV) is a
measurement of the proportion of
blood that is made up of cells.
• PCV also Know as Hematocrit

5. Principle :
• Hematocrit is usually determined by a spined blood-filled capillary tube in
a Centrifuge
Specimen :
• Venous blood anticoagulant with EDTA or capillary blood
collected directly into heparinized capillary tube can be used
• Specimens should be centrifuged within 6 hours of collection

6. Reagent and
Equipment :
• Capillary Tubes
• Heparinized for finger sticks (red tip)
• Plain for anticoagulatet blood (blue tip)
• Claye-type tube sealant
• Microhematocrit Centrifuge
• Microhematocrit reader

7. Procedures :
• Fill Two capillary tubea approximately three quarters full with blood anticoagulated EDTA or
Heparin
• Wipe any excess blood from the outside of tube
• Seal the end of the tube with the colored ring with nonabsorbent clay
• Balance thw tubes in the centrifuge with the clay end Facing the outside away from the centre,
touching the gasket
• Tighten the head cover on the centrifuge and close the top
• Activate the centrifuge for 5minutes Between 10,000 rpm to 15,00 rpm
• Determine the HCT by using a microhematocrit reading device
• Read the level of RBC packing

9. Hematocrit Reading :
• Different stages has different Ranges of Hematocrit or PCV
• Such as ;
• New Born 53-65%
• Infant / Child 30-43%
• Adult Male 42-52%
• Adult female 37-47%

10. High Hematocrit
Level :
• Some Factors may cause a rise in hematocrit value such as :
• Polycythemia
• Heart or kidney problems
• Intake of anabolic Steroids
• Dehydration
• Diarrhea
• Lung problems
• Smoking or Burns

11. Low Hematocrit
Level :
•The most common symptoms of low
hematocrit levels are : Fatigue ,
weakness, dizzness and low hemoglobin
level and pregnancy is commonly
associated with a decreased hematocrit
ranges
Other Causes are :
• Anemia
• Hemolysis
• Bleeding
• Renal failure
• Nutritional deficiencies and Medications

12. https://www.youtube.com/watch?v=ofMTeoYSNn4
https://www.youtube.com/watch?v=ofMTeoYSNn4
https://www.youtube.com/watch?v=ofMTeoYSNn4