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Of the three organisms Escherichia coli, Bacillus anthracis, and Micrococcus luteus, _____
would be likely to stain improperly due to the culture being old. This occurs because of the
degradation of_____ (compound) by enzymes called _____ which accumulate with aging. In
colonies of the spore forming genus Clostridium, spores would be more likely to form in the
_____ of the colony. Bacteria in the genus _____, when grown on solid media containing
cysteine and iron, would have colonies that may be black in the _____and white in the_____.
Solution
Cell wall
Peptidoglycan
Lysozyme or autocatalytic enzymes
Center or terminal of the colony
Legionella genus
Thiosulfate citrate bile salts sucrose agar
Buffered charcoal yeast extract agar

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