Oasis PRiME is a new reversed-phase solid phase extraction sorbent that provides cleaner, simpler, and faster sample cleanup. It removes over 95% of common matrix interferences like salts, proteins, fats, and phospholipids using a generic 3-step protocol without requiring SPE expertise. The sorbent also removes 99% of phospholipids compared to 50% for competitive sorbents. For basic compound extraction, Oasis PRiME MCX uses a 3 or 4-step protocol to remove 95-98% of phospholipids, simplifying mixed-mode cation exchange methods. Both sorbents provide optimized flow rates for more consistent and predictable processing times.

1. [ OASIS PRIME ]
SIMPLER. CLEANER. FASTER.
Sample Clean-up with Oasis PRiME
Process
Robustness
improvements in...
Matrix Effects
Ease of Use
What is
PRiME?
©2018 Waters Corporation. Waters, The Science of What’s Possible, and Oasis are
registered trademarks of Waters Corporation. All other trademarks are the property
of their respective owners. 720006177EN January 2018 LM-SIG
Remaining Phospholipids
Competitive Reversed-Phase
SPE Sorbent
Oasis PRiME
HLB
0%
70%
80%
90%
100%
110%
120%
60%
50%
40%
30%
20%
10%
5.0%
3-STEP PROTOCOL >> No SPE expertise required
CLEAN-UP AND CONCENTRATE ACIDS, BASES, AND NEUTRALS FROM COMPLEX
BIOLOGICAL SAMPLE MATRICES >> Concentrate samples up to 15X
A reversed-phase, solid-phase extraction device
Cleaner
Removes more than
95% of common
matrix interferences
such as salts, proteins,
fats, pigments, and
phospholipids,
with the generic
3-step protocol
Elute: 90/10: Acetonitrile/MeOH
Wash: 5% MeOH
Load: Pre-treated aqueous sample
Simpler
Easy protocols that result
in cleaner samples
No condition and
equilibration steps
are required
Easy to implement into
laboratory workflows
without SPE expertise
Faster
More even flows across
cartridges and plates
with less plugging
Faster overall
processing with
the elimination
of condition and
equilibration steps
Oasis PRiME
HLB removes
95%of phospholipids compared
to other reversed-phase
solid-phase extraction
sorbents
Patent Pending
Designed to simplify
solid-phase extraction
Oasis PRiME HLB
Remaining Phospholipids
Oasis PRiME
MCX 4-Step
Protocol
Oasis PRiME
MCX 3-Step
Protocol
Oasis MCX
Generic Protocol
0
7000000
8000000
9000000
10000000
6000000
5000000
4000000
3000000
2000000
1000000
• 100% Methanol
• 5% NH4OH in methanol
• Pre-treated sample
• Typically acidified by diluting 1:1
(v/v) with 4% H3PO4 in water
• 100 mM Ammonium formate
with 2% formic acid in water
Elute
Wash 2
Wash 1
Load
**Contains an extra wash step that can be used to remove additional matrix
interferences if needed.
4-STEP OASIS PRiME MCX PROTOCOL**
• 5% NH4OH in methanol
• Pre-treated sample
• Diluted 1:1 (v/v) with an aqueous
solution of 200 mM Ammonium
formate with 4% H3PO4
• 100% Methanol
Elute
Wash 1
Load
*The sample is diluted 1:1 with 200 mM Ammonium formate with 4% H3PO4,
making a final concentration of 100 mM Ammonium formate and 2% H3PO4.
3-STEP OASIS PRiME MCX PROTOCOL*
3-STEP PROTOCOL >>
Need a higher level of specificity for basic compounds?
Mixed-mode (Reversed-phase/Cation-eXchange)
3-step
method removes
95%of phospholipids
4-step
method removes
98%of phospholipids compared
to Oasis MCX with the
generic protocol
Simplify your steps by compared to competitive mixed-mode
cation-exchange protocols50%
For more information visit
waters.com/prime
Simpler method removes 99% of
phospholipids compared to protein
precipitation, a major cause of matrix
effects, ion suppression, shorter column
lifetimes, increased MS maintenance, and
higher variability in LC-MS quantification
Oasis PRiME MCX is QC’d with this
protocol for phospholipid removal
CleanerSimpler
A defined, generic, 3- or 4-step
SPE protocol based on the water
wettable Oasis MCX strong
Cation-eXchange/reversed-phase
sorbent that does not require
extensive method development
Allows targeted clean-up
of basic compounds
Faster
Cartridges and plates are
designed with a manufacturing
optimization to increase flow
reproducibility across devices,
making processing time
more predictable
No conditioning and
equilibration steps are required
Oasis PRiME MCX