NPR-1 is required for regulating anxiety, food consumption, social and solitary feeding, in regulating social behaviour and sensing pain. Indirectly, food is the main factor in the solitary or social behaviour. The nematode with wild type npr-1, move slowly and disperse all over, while mutant npr-1 move rapidly and aggregate together at the border (the phenomen called as bordering). This behaviour in locomotion is the main cause of social or solitary behaviour. Solution NPR-1 is required for regulating anxiety, food consumption, social and solitary feeding, in regulating social behaviour and sensing pain. Indirectly, food is the main factor in the solitary or social behaviour. The nematode with wild type npr-1, move slowly and disperse all over, while mutant npr-1 move rapidly and aggregate together at the border (the phenomen called as bordering). This behaviour in locomotion is the main cause of social or solitary behaviour..