This document is a lab report for a COVID-19 RT-PCR test. It indicates that the test for the sample from Miss. Nidhi Subash, a 24-year-old female, came back negative for SARS-CoV-2 RNA. The test was performed at Sankar's Healthcare Scans and Diagnostics in Alappuzha, Kerala, India and analyzed samples from a nasopharyngeal/oropharyngeal swab using real-time RT-PCR. The report provides background on SARS-CoV-2 and the test methodology, and notes that a negative result does not rule out infection and should be interpreted along with clinical observations.

1. Sample Drawn
Reported
Lab ID
Name
Age / Sex
Ref. By
: Miss. NIDHI SUBASH
: 24 Years / Female
:
: VNDC-22-19975
: 21-Jan-2022 10:00 pm
: 21-Jan-2022 10:00 pm
: 22-Jan-2022 3:16 am
Client
Passport No
: S PATH LAB EKM
:
Registered
KALA ARCADE, NEAR T D MEDICAL COLLEGE HOSPITAL ,
VANDANAM, ALAPPUZHA - 688001
Phone : 9526204488
MOLECULAR BIOLOGY
Result
Test
COVID-19 RT PCR
N Gene
RdRp Gene
SARS-CoV-2 RNA
FINAL REPORT
NOT DETECTED
NOT DETECTED
NOT DETECTED
NEGATIVE
ICMR Reg No: SHCSDVAAK
Test Performed at Sankar’s Healthcare Scans and Diagnostics, Vandanam,
AS
la
p
p
e
p
c
u
im
zh
e
a
n
. :
Method: This is a real-time RT-PCR based test intended for the qualitative detection of nucleic acid from the Severe Acute Respiratory
Syndrome Coronavirus 2 (SARS-CoV-2) in the respiratory specimens. This test involves two principle steps: extraction of RNA from the
patient specimens, followed by one-step reverse transcription and PCR amplification with SARS-CoV-2 specific primers and real-time
detection with 2019-nCoV specific probes.
Background: SARS-CoV-2 is an enveloped and positive-sense single stranded RNA (+ssRNA) virus that belongs to Betacoronavirus genera
together with two highly pathogenic viruses, SARS-CoV and MERS-CoV. The disease caused by SARS-CoV-2, coronavirus disease 2019
(COVID-19)shows varying symptoms amongst individuals, ranging from asymptomatic infection to severe respiratory failure. The
SARS-CoV-2 infection mainly presents flu-like symptoms such as fever, cough and asthenia.
Interpretation:
DETECTED status indicates the presence of SARS-CoV-2 RNA in the patient sample. Clinical correlation with patient history and other
diagnostic findings is recommended.
NOT DETECTED status indicates SARS-CoV-2 RNA is absent or below detection limit of this test. The ‘not detected’ status do not rule out
SARS-CoV-2 infection and hence, should not be used as the sole basis for patient management decisions. This result must be combined
with clinical observations, patient history, and epidemiological information. Further, a false negative results may occur due to various
causes- inadequate specimen quality, specimen collected too early or too late, specimen improperly handled or transported or presence of
PCR inhibitors. Other upper respiratory tract specimens should be collected and tested when a highly suspected COVID-19 patient tests
negative using a single upper respiratory tract specimen. The lower respiratory tract specimens may be collected together and tested. Repeat
testing may be done on a new specimen after a gap of 48 - 72 hours after collection of first specimen, if required.
NASOPHARYNGEAL/OROPHARYNGEAL SWAB
DR. R. MANIKKUMAR
M Sc (Med.Bio) Ph.D(Med.Bio)
Specialist, Molecular Diagnostics
SRF NO : 2481/ALP/2021057942
Note: Results to be interpreted in conjunction with medical history, clinical presentation & other findings.
*----------------------- END OF REPORT -----------------------*
Page 1 of 1
User : SABITHA