The cell membrane separates a cell from its environment and regulates what passes in and out through selective permeability. It helps maintain homeostasis through balancing pH, temperature, glucose, and water levels via active and passive transport. Passive transport moves particles down their concentration gradient without energy, through diffusion, facilitated diffusion, and osmosis. Active transport moves particles against their gradient by using ATP energy and protein pumps.
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Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
2. Function of the Cell Membrane:
• Cell membrane separates the components of a cell
from its environment—surrounds the cell
• “Gatekeeper” of the cell—regulates the flow of
materials into and out of cell—selectively permeable
• Cell membrane helps cells maintain homeostasis—
stable internal balance
3. The Cell Membrane & Homeostasis
• The cell membrane is
responsible for maintaining
homeostasis (home-E-O-
Stay-sis) within the cell
• Homeostasis is a stable,
internal environment
• The cell membrane
maintains homeostasis
through balancing the pH,
temperature, glucose
(sugar intake), water
balance
• It does this through active
and passive transport
In homeostasis, everything is PERFECT
4. pH and homeostasis
• The pH of a solution tells how
acidic or basic it is.
pH ranges from a scale to 0-14
• Solutions with a pH from 0-6
are acidic
• Solutions with a pH of 8-14
are basic
• Solutions with a pH of 7 are
Neutral.
If a solution’s pH is unbalanced,
it is corrected with a BUFFER.
5. Is it Basic, Acidic, or Neutral?
• Orange juice w/ a pH of 2
• Gastric juices (stomach juices) w/ a pH of 1
• Tap water w/ a pH of 7
• Sodium hydroxide w/ a pH of 10
• Ammonia w/ a pH of 14
1 (acid)………………6 7(neutral) 8…………………14 (basic)
6. Cell Membrane aka “The Phospholipid Bilayer”
• ALL cells have a cell membrane made of Phosphate,
proteins, and lipids
•That’s why it’s called the Phospholipid Bilayer
Cell Membrane
lipid bilayer
protein channel
protein pump
Layer 1
Layer 2
All Cells have a cell (plasma membrane):
• Prokaryotes (have a cell wall + cell membrane)
• Eukaryotes:
• a) Animal Cells ( cell membrane only)
• b) Plant cells (cell membrane + cell wall)
7. The cell membrane in detail
• It’s a double layer (bilayer)
of phosphates, and fats
(lipids)
• A single phospholipid has
hydrophilic (water loving)
phosphate heads AND
hydrophobic (water hating)
fatty acid tails
• The cell membrane both
repels and attracts water
through the membrane at
the same time
HydroPHILIC head
hydroPHOBIC tails
8. Passive Transport
A process that does not require energy to move
molecules from a HIGH to LOW concentration
Diffusion
Facilitated Diffusion (uses proteins to push
particles across)
Osmosis
9. •Diffusion is the movement of small particles across the
cell membrane like the cell membrane until homeostasis
is reached.
• Facilitated diffusion requires the help of carrier and
channel proteins
These particles move from an area of high concentration
to an area of low concentration.
outside of cell
inside of cell
10. • Examples of diffusion: spraying aerosols, and perfumes.
• High concentration (inside of the can)—the molecules are
packed tightly together….
• To a LOW concentration – when sprayed, the molecules
are released to a more free environment
• The particles SPREAD OUT
11. • Osmosis is the movement of water through a selectively
permeable membrane like the cell membrane
Water moves across the cell membrane from an area of
high concentration to an area of low concentration.
Semi-permeable
membrane is
permeable to water,
but not to sugar
12. Hypertonic Solutions: contain a high concentration of solute
relative to another solution (e.g. the cell's cytoplasm). When
a cell is placed in a hypertonic solution, the water diffuses
out of the cell, causing the cell to shrivel.
Hypotonic Solutions: contain a low concentration of solute
relative to another solution (e.g. the cell's cytoplasm). When
a cell is placed in a hypotonic solution, the water diffuses
into the cell, causing the cell to swell and possibly explode.
Isotonic Solutions: contain the same concentration of solute
as another solution (e.g. the cell's cytoplasm). When a cell is
placed in an isotonic solution, the water diffuses into and
out of the cell at the same rate. The fluid that surrounds the
body cells is isotonic.
13. Osmosis Concentration
• Hypertonic: the water or solution OUTSIDE of
the cell is saltier than the INSIDE of the cell.
• Hyper = “more” ore “above”
• This will cause it to shrivel, and shrink
• Ex. Pouring salt on a slug will cause it to shrink
14. Osmosis Concentration
• Hypotonic: the water or solution OUTSIDE of
the cell
• Hypo means “less than” or “below”
• A hypotonic solution will cause the cell to take
in water, and swell
15. Osmosis Concentration
• Isotonic: the water outside of the cell has an
EQUAL amount of salt as the water INSIDE of
the cell.
• Iso means “equal”
• Will cause NO CHANGE in cell size
18. Types of Active Transport
• Active transport uses ENERGY (ATP)
• EXOcytosis = how materials EXIT the cell (how the
cell uses the bathroom)
• ENDOcytosis = how materials ENTER the cell (cell
eating/engulfing)
• PINOcytosis= how small materials ENTER the cell
(cell eating/engulfing)
• PHAGOcytosis = how larger materials ENTER the
cell (cell eating/engulfing)
19. Active Transport
Active transport is the movement of molecules from LOW to HIGH
concentration.
Energy is required as molecules must be pumped against the
concentration gradient.
Proteins that work as pumps are called protein pumps.
Ex: Body cells must pump carbon dioxide out into the surrounding
blood vessels to be carried to the lungs for exhale. Blood vessels are
high in carbon dioxide compared to the cells, so energy is required
to move the carbon dioxide across the cell membrane from LOW to
HIGH concentration.
outside of cell
inside of cell
Carbon Dioxide
molecules
20. NO ENERGY NEEDED:
Diffusion
Osmosis
Facilitated Diffusion
ENERGY NEEDED:
Active Transport
ANALOGY: Passive Transport vs. Active Transport
Passive Transport: Like
going DOWNHILL
Active Transport: like going
UPHILL