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Cultivating Mushrooms in a
Cold Room Chamber: A Step-
by-Step Guide
Presentation by Awotech

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Mushroom cultivation is a captivating and
rewarding endeavor that doesn’t always
require a traditional setup. With a cold room
chamber, you can create an ideal
environment for growing these fungi. Let’s
delve into the step-by-sep process of
cultivating mushrooms in a cold room
chamber.

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Understanding the Cold Room
Chamber
A cold room chamber provides
controlled conditions with lower
temperatures and controlled humidity,
which are beneficial for certain
mushroom varieties. Ideal
temperatures vary depending on the
mushroom species, but generally fall
between 45°F to 65°F (7°C to 18°C).

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Selecting Mushroom Varieties
Different mushroom species thrive in various
conditions. Varieties like oyster, shiitake, and
lion's mane can flourish in cooler environments,
making them suitable choices for cold room
cultivation.

5. Preparing the Cold Room Chamber
Cleanliness: Ensure the chamber is thoroughly
cleaned and sanitized to prevent contamination.
Sterilize surfaces and equipment to create a sterile
environment.
Humidity Control: Install a humidifier or use trays
of water to maintain optimal humidity levels. Aim
for around 90% humidity for most mushroom
species.
Temperature Control: Utilize a thermometer to
monitor and regulate the temperature. A
thermostat-controlled heater or cooler can help
maintain the desired temperature range.
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6. Mushroom Cultivation Steps
Spawn Preparation: Obtain mushroom spawn
from a reputable supplier or prepare your own
by mixing spores with a substrate such as
sawdust, straw, or compost.
Substrate Preparation: Sterilize the
substrate by pasteurizing or heat-treating
it to eliminate any competing organisms.
Fill containers or bags with the substrate
and spawn mixture.
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Inoculation: Once the substrate is prepared, inoculate it
with the mushroom spawn. This can be done by mixing
the spawn thoroughly into the substrate or layering it
within the container.
Incubation: Place the inoculated substrate in the cold
room chamber. During this phase, the mycelium will
colonize the substrate. Maintain proper humidity and
temperature levels.

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Fruiting Stage: After the substrate is fully colonized,
initiate the fruiting stage by adjusting temperature
and humidity conditions. Mushrooms will start to
develop and grow.
Harvesting: Harvest mushrooms when they reach
maturity. Carefully cut or twist them off the substrate
to avoid damaging the mycelium.

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Maintenance and Troubleshooting
Monitoring: Regularly check temperature, humidity,
and the development of mushrooms.
Pest and Contamination Control: Keep the environment
clean and watch for any signs of contamination or
pests. Remove any contaminated substrates promptly.
Air Circulation: Ensure proper air circulation to prevent
the buildup of carbon dioxide and encourage healthy
mushroom growth.

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Conclusion
Cultivating mushrooms in a cold room
chamber requires attention to detail, but the
process is highly rewarding. From selecting the
right mushroom species to maintaining the
ideal environment, each step contributes to
successful cultivation. With patience and
diligence, you can enjoy a bountiful harvest of
flavorful, home-grown mushrooms.

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Final Thoughts
The versatility of a cold room chamber allows mushroom
enthusiasts to explore and experiment with different species,
fostering a deeper understanding of the fascinating world of
mushroom cultivation. Whether for personal consumption or
commercial purposes, this method offers an exciting opportunity to
grow fresh, organic mushrooms year-round.
Creating a controlled environment like a cold room chamber
for mushroom cultivation can be a fantastic way to grow them
even in less conventional settings. With attention to detail and
the right conditions, you can yield a bountiful harvest of
delicious mushrooms.

12. For inquiries or follow-ups
Get In Touch
With Us
Company Address
Phone Number
Email Address
226,Neerav Nikunj Sikandra Agra UP
9411466708
awotechindia@gmail.com
Web Address
www.awotech.in

13. Thank
You
For Attention
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