MMP's and the Role of Zinc in Wound Healing
•
4 likes•1,340 views
Matrix metalloproteinase (MMP) and Zinc play an important role in wound healing. As MMP's are dependent upon Zinc, you can't have one without the other. Without MMP's you can not have collenganese activity (or other endogenous proteolytic enzymes) resulting in a wound that will not heal. For more information on this topic and many others regarding wound healing, check out our other presentations here.
Report
Share
Report
Share
Recommended
Abstract
1) Protein phosphatase 5 (PP5) regulates the differentiation of mesenchymal stem cells into either adipocytes or osteoblasts through phosphorylation of PPARγ and RUNX2. 2) Mice deficient in PP5 have increased bone formation and decreased fat accumulation in bone marrow. 3) Experiments analyzing mice with tissue-specific knockout of PP5 in adipose tissue or long bones found that loss of PP5 in adipose tissue increased trabecular bone, while loss in long bones increased cortical bone thickness, implicating PP5 in regulating the balance between bone and fat formation in different tissues.
presentation
1) Protein phosphatase 5 (PP5) regulates the differentiation of mesenchymal stem cells into either adipocytes or osteoblasts through phosphorylation of PPARγ and RUNX2.
2) Mice deficient in PP5 showed increased bone formation and decreased marrow adipocytes, suggesting PP5 promotes adipogenesis over osteogenesis.
3) Experiments on mice with tissue-specific PP5 knockout found loss of PP5 in bone led to increased cortical bone thickness while loss in adipose tissue led to increased trabecular bone formation, demonstrating PP5 regulates the balance between bone and fat formation in different tissues.
073 collagenases vs collagen
This document discusses research on the roles of collagen and collagenases in atherosclerotic plaque vulnerability. It presents several hypotheses: 1) that human and experimental plaques contain collagenases, 2) that lipid lowering improves features associated with vulnerability by reducing macrophages and collagenase expression while increasing collagen, and 3) that collagenolysis regulates collagen accumulation in plaques of genetically altered mice. Several studies are described that provide evidence supporting these hypotheses, finding that collagenases are expressed in human and animal plaques, lipid lowering reduces vulnerability features in rabbits by altering collagenase and collagen levels, and collagenase-resistant mice have altered collagen content in plaques. The research suggests collagenases play a critical role in plaque collagen metabolism and vulnerability.
The balancee of proteolysis
This document discusses research on the roles of collagen and collagenases in atherosclerotic plaque vulnerability. It presents several hypotheses: 1) that human and experimental plaques contain collagenases, 2) that lipid lowering improves features associated with vulnerability by reducing macrophages and collagenase expression while increasing collagen, and 3) that collagenolysis regulates collagen accumulation in plaques of genetically altered mice. Several studies are described that provide evidence supporting these hypotheses, showing that collagenases are expressed in human and animal plaques, lipid lowering reduces vulnerability features in rabbits, and collagenase-resistant mice have altered collagen content in plaques. The research suggests collagenases play a critical role in plaque collagen metabolism and vulnerability.
Masanori aikawa powerpoint
The document discusses collagenases and their role in plaque vulnerability and stability. It presents evidence that:
1) Macrophages in human and experimental atherosclerotic plaques express collagenases like MMP-1, MMP-8, and MMP-13 which can degrade collagen.
2) Lipid lowering in rabbits reduces macrophage accumulation and collagenase expression in plaques, while increasing collagen content, suggesting collagenases regulate collagen balance.
3) Mice with genetically reduced collagenase activity have altered collagen content in plaques, providing direct evidence collagenases regulate plaque collagen levels.
CVA Biology I - B10vrv4133
Mutations are changes in genetic information that can be inherited. There are two types: gene mutations involving changes in single genes, and chromosomal mutations involving changes to whole chromosomes. Gene mutations include point mutations like substitutions, insertions, and deletions. Chromosomal mutations involve changes in chromosome number or structure like deletions, duplications, inversions, and translocations. Mutations can have harmful effects by disrupting gene function, but can also occasionally provide benefits by generating genetic variation and helping organisms adapt.
Nutrição e Inflamação, Maio, 2013
The document discusses the immune system and inflammation. It begins with an overview of the cellular components of the immune system, including macrophages, monocytes, basophils, eosinophils, neutrophils, and lymphocytes. It then discusses the process of exercise-induced muscle damage and the subsequent muscle inflammation and regeneration process. This involves leukocyte infiltration, cytokine production, phagocytosis to remove damaged muscle fibers, and satellite cell proliferation to aid regeneration over 1-2 weeks after exercise. NSAIDs are shown to inhibit this inflammatory process and blunt the activity of satellite cells that aid muscle repair.
FOS ALS '13 Poster (final)
This study examined the effects of cerium oxide nanoparticles (CeO2 NPs) on disease progression in a transgenic mouse model of amyotrophic lateral sclerosis (ALS). The mice expressed a mutation in the SOD1 gene associated with familial ALS. Following disease onset, mice were given injections of either saline (control) or CeO2 NPs. Mice treated with CeO2 NPs showed improved motor performance and increased survival time, particularly female mice. The results suggest CeO2 NPs may be a potential treatment for slowing progression of ALS and other neurodegenerative diseases associated with oxidative stress.
Recommended
Abstract
1) Protein phosphatase 5 (PP5) regulates the differentiation of mesenchymal stem cells into either adipocytes or osteoblasts through phosphorylation of PPARγ and RUNX2. 2) Mice deficient in PP5 have increased bone formation and decreased fat accumulation in bone marrow. 3) Experiments analyzing mice with tissue-specific knockout of PP5 in adipose tissue or long bones found that loss of PP5 in adipose tissue increased trabecular bone, while loss in long bones increased cortical bone thickness, implicating PP5 in regulating the balance between bone and fat formation in different tissues.
presentation
1) Protein phosphatase 5 (PP5) regulates the differentiation of mesenchymal stem cells into either adipocytes or osteoblasts through phosphorylation of PPARγ and RUNX2.
2) Mice deficient in PP5 showed increased bone formation and decreased marrow adipocytes, suggesting PP5 promotes adipogenesis over osteogenesis.
3) Experiments on mice with tissue-specific PP5 knockout found loss of PP5 in bone led to increased cortical bone thickness while loss in adipose tissue led to increased trabecular bone formation, demonstrating PP5 regulates the balance between bone and fat formation in different tissues.
073 collagenases vs collagen
This document discusses research on the roles of collagen and collagenases in atherosclerotic plaque vulnerability. It presents several hypotheses: 1) that human and experimental plaques contain collagenases, 2) that lipid lowering improves features associated with vulnerability by reducing macrophages and collagenase expression while increasing collagen, and 3) that collagenolysis regulates collagen accumulation in plaques of genetically altered mice. Several studies are described that provide evidence supporting these hypotheses, finding that collagenases are expressed in human and animal plaques, lipid lowering reduces vulnerability features in rabbits by altering collagenase and collagen levels, and collagenase-resistant mice have altered collagen content in plaques. The research suggests collagenases play a critical role in plaque collagen metabolism and vulnerability.
The balancee of proteolysis
This document discusses research on the roles of collagen and collagenases in atherosclerotic plaque vulnerability. It presents several hypotheses: 1) that human and experimental plaques contain collagenases, 2) that lipid lowering improves features associated with vulnerability by reducing macrophages and collagenase expression while increasing collagen, and 3) that collagenolysis regulates collagen accumulation in plaques of genetically altered mice. Several studies are described that provide evidence supporting these hypotheses, showing that collagenases are expressed in human and animal plaques, lipid lowering reduces vulnerability features in rabbits, and collagenase-resistant mice have altered collagen content in plaques. The research suggests collagenases play a critical role in plaque collagen metabolism and vulnerability.
Masanori aikawa powerpoint
The document discusses collagenases and their role in plaque vulnerability and stability. It presents evidence that:
1) Macrophages in human and experimental atherosclerotic plaques express collagenases like MMP-1, MMP-8, and MMP-13 which can degrade collagen.
2) Lipid lowering in rabbits reduces macrophage accumulation and collagenase expression in plaques, while increasing collagen content, suggesting collagenases regulate collagen balance.
3) Mice with genetically reduced collagenase activity have altered collagen content in plaques, providing direct evidence collagenases regulate plaque collagen levels.
CVA Biology I - B10vrv4133
Mutations are changes in genetic information that can be inherited. There are two types: gene mutations involving changes in single genes, and chromosomal mutations involving changes to whole chromosomes. Gene mutations include point mutations like substitutions, insertions, and deletions. Chromosomal mutations involve changes in chromosome number or structure like deletions, duplications, inversions, and translocations. Mutations can have harmful effects by disrupting gene function, but can also occasionally provide benefits by generating genetic variation and helping organisms adapt.
Nutrição e Inflamação, Maio, 2013
The document discusses the immune system and inflammation. It begins with an overview of the cellular components of the immune system, including macrophages, monocytes, basophils, eosinophils, neutrophils, and lymphocytes. It then discusses the process of exercise-induced muscle damage and the subsequent muscle inflammation and regeneration process. This involves leukocyte infiltration, cytokine production, phagocytosis to remove damaged muscle fibers, and satellite cell proliferation to aid regeneration over 1-2 weeks after exercise. NSAIDs are shown to inhibit this inflammatory process and blunt the activity of satellite cells that aid muscle repair.
FOS ALS '13 Poster (final)
This study examined the effects of cerium oxide nanoparticles (CeO2 NPs) on disease progression in a transgenic mouse model of amyotrophic lateral sclerosis (ALS). The mice expressed a mutation in the SOD1 gene associated with familial ALS. Following disease onset, mice were given injections of either saline (control) or CeO2 NPs. Mice treated with CeO2 NPs showed improved motor performance and increased survival time, particularly female mice. The results suggest CeO2 NPs may be a potential treatment for slowing progression of ALS and other neurodegenerative diseases associated with oxidative stress.
ECCLU 2011 - N. Clarke - Bone targeted agents
1. The document discusses bone metabolism and prostate cancer, describing how factors like RANK ligand and osteoprotegerin regulate the balance between bone formation and resorption.
2. It summarizes a clinical trial comparing denosumab, a RANK ligand inhibitor, to zoledronic acid for treating bone metastases in prostate cancer patients. Denosumab delayed time to first skeletal-related event compared to zoledronic acid and reduced risk of multiple events.
3. Rates of adverse events were generally similar between the treatments, though denosumab resulted in fewer acute phase reactions and more cases of osteonecrosis of the jaw compared to zoledronic acid.
Extracellular vesicles from human liver stem cells restore arginosuccinate sy...
This study investigated using extracellular vesicles (EVs) derived from normal human liver stem cells (HLSCs) to treat argininosuccinate synthase deficiency (ASS1), which causes citrullinemia type I. The study found that:
1) EVs from HLSCs contained wild-type ASS1 protein, mRNA, and DNA.
2) When EVs from normal HLSCs were applied to ASS1-deficient hepatocytes differentiated from patients' HLSCs, they increased urea production, ASS1 enzymatic activity, and decreased DNA mutations in the recipient cells.
3) This suggests EVs derived from stem cells have potential to correct ASS1 enzyme deficiencies by transferring functional proteins and
Effect Of Ethanol In Oligodendrocytes, research miami
This study aimed to determine if ethanol's protective effects against oxidative stress in injured organisms is due to increasing cAMP signaling pathways. The researchers hypothesized that treating cells with ethanol after injury would increase cAMP, PKA, and CREB production, decreasing cell death. Their methods involved inducing spinal cord injuries in rats, culturing cells from the spinal cord and brain, treating cells with ethanol and hydrogen peroxide, and analyzing PKA and CREB levels and cell apoptosis. Their results showed that treating cells with 5% ethanol for 24 hours after oxidative stress increased PKA and CREB levels and reduced cell death. They concluded that combining a 5% ethanol treatment for 24 hours with a pharmacological agent could be a functional treatment to reduce paralysis
Following the Evolution of New Protein Folds via Protodomains
Protein evolution proceeds through genetic mechanisms, but selection acts on biological assemblies. I define a protodomain as a minimal independently evolving unit with conserved structure. Protodomain rearrangements have minimal impact on biological assemblies, so they represent a valid evolutionary path through fold space.
These slides are from my Candidacy Exam on Jan 28, 2013 at University of California, San Diego. It discusses my current research in Philip Bourne's lab, as well as proposes research for my thesis over the next two years. An audio version is available at http://www.scivee.tv/node/57082
ASBMB_Poster_3_25_2015_Jake
This document summarizes research analyzing relationships between lysine ubiquitylation and acetylation sites from proteomic datasets in Arabidopsis thaliana proteins. The research compared ubiquitylation and acetylation sites identified in previous publications and found that 9 non-nuclear proteins experienced both post-translational modifications. Most of these proteins were located in energy-associated organelles. While this provides support for potential cross-talk between ubiquitylation and acetylation, more acetylation and ubiquitylation data is still needed to further investigate these relationships and whether acetylation directly or indirectly influences ubiquitylation.
ASBMB_Poster_3_25_2015_Jake
This document summarizes research analyzing relationships between lysine ubiquitylation and acetylation sites in Arabidopsis thaliana proteins from proteomic datasets. The researchers compared ubiquitylation and acetylation sites identified in previous publications and found that 9 proteins experienced both post-translational modifications. Most of these proteins were located in energy-associated organelles, supporting the hypothesis that ubiquitylation and acetylation interactions could influence metabolic pathways. Future work is needed to identify more acetylation and ubiquitylation sites to better understand potential direct or indirect influences between the two modifications.
MSBMB 2016 conference poster
The document describes cloning, expressing, and purifying a glutathione S-transferase (GST) from Pseudomonas putida KT2440 and investigating its potential role in iron metal bioremediation. Key findings include:
- The GST gene (PP_2933) was cloned, expressed producing a 31kDa protein, and purified using ion exchange chromatography.
- The purified GST showed activity with certain substrates, including ethacrynic acid and cumene peroxide, indicating a role in oxidative stress response.
- Cells expressing the recombinant GST showed increased resistance to iron, suggesting it may bind iron complexes and aid in bioremediation of iron toxicity.
JBC1
This study investigated the degradation of soluble and myofibrillar proteins in rabbit muscle extracts by the ATP-ubiquitin-proteasome pathway. The key findings were:
1) Degradation of soluble muscle proteins was stimulated up to 6-fold by ATP and inhibited by removal of proteasomes or addition of proteasome inhibitors, indicating these proteins are substrates of the ATP-ubiquitin-proteasome pathway.
2) Purified myofibrillar proteins (myosin, actin, troponin, tropomyosin) added to the extracts were also degraded in an ATP-dependent manner, showing they can serve as substrates of this pathway.
3)
DEREKCV1216most current
This document provides biographical and professional details about Derek J. Milner, including his education, research experience, teaching experience, awards, publications, and presentations. It outlines his educational background, including a Ph.D. in Molecular and Cellular Biology from Baylor College of Medicine and a B.S. in Biochemistry from the University of Iowa. It then details his extensive research experience in regenerative biology and tissue engineering at the University of Illinois over the past 15 years, focusing on projects involving muscle regeneration, stem cells, and animal models of bone and cartilage repair.
PNAS
1) Rates of ubiquitin conjugation (attachment of ubiquitin molecules) to proteins increase in muscles undergoing atrophy (wasting away), largely due to activation of the N-end rule pathway.
2) The N-end rule pathway is responsible for most changes in ubiquitin conjugation rates in atrophying muscles from tumor-bearing or septic rats. Inhibitors of this pathway suppressed the increased ubiquitin conjugation seen in these conditions.
3) Ubiquitin conjugation rates increase in atrophying conditions like cancer cachexia and sepsis but decrease in hypothyroid muscles, and this response is mediated by the N-end rule pathway.
ATP Review
ATP (adenosine triphosphate) is the molecular unit that stores and transports energy within cells. It fuels enzymes and is present in all cells, providing energy for physiological processes. ATP is continuously recycled in the body, turning over its own body weight equivalent daily. Several studies have found that ATP supplementation can help reduce muscle fatigue, improve force output during exercise, increase muscle mass, strength and power, and provide small ergogenic effects on muscular strength under some conditions. However, more research is needed before recommending ATP supplementation to athletes seeking ergogenic aids.
poster final
This study aimed to validate an osteocyte-specific growth hormone receptor (GHR) knockout mouse model generated using the Cre/loxP system with Cre recombinase driven by the dentin matrix protein 1 (DMP1) promoter. The researchers found that while the DMP1-derived Cre mediated GHR knockout specifically in osteocytes as intended, GHR gene recombination was also detected in muscle tissue. They concluded that the DMP1-GHRKO mouse model is valid for studying the role of GHR in osteocytes, but the bone phenotype needs to be characterized with the knowledge that the gene recombination also occurred in muscle.
Smith_CV_Full
Austin E. Smith is a PhD candidate in chemistry at UNC-Chapel Hill studying protein amide-hydrogen exchange in vitro and in living cells using NMR. He received his BS from Furman University and his MS from Furman, studying inhibition of HMGA1 proteins by distamycin A using NMR and fluorescence spectroscopy. His research experience includes protein expression, purification, calorimetry, fluorescence, and molecular biology techniques. He has mentored undergraduates, post-bacs, and graduate students in his lab.
Hum. Mol. Genet.-2014-Goldstein-hmg-ddu390
1) Muscle injury in mice lacking the protein γ-sarcoglycan causes an increase in SMAD signaling, which contributes to transforming growth factor β (TGFβ) signaling.
2) Reducing SMAD signaling through introducing a heterozygous mutation of the gene SMAD4 in γ-sarcoglycan mice led to improved body mass, cardiac function, and muscle force compared to γ-sarcoglycan mice.
3) While fibrosis was not reduced, membrane damage was decreased, suggesting intracellular SMAD4 targets are important for mediating muscle and heart dysfunction in muscular dystrophy.
Osteoimmunology
This document discusses osteoimmunology, which examines the interactions between the bone and immune systems. It notes that bone cells and immune cells share a common origin and influence each other bidirectionally. Specifically, it outlines that osteoblasts regulate the hematopoietic stem cell niche that gives rise to immune cells, while osteoclasts are related to macrophages and dendritic cells. Additionally, cytokines and other signaling molecules regulate both bone and immune cell activity. A key example is RANKL, produced by osteocytes, which contributes to bone loss and influences lymphocyte development by activating RANK on osteoclast progenitors.
SCE Presentation
This study investigated the structural stability of the cystic fibrosis transmembrane conductance regulator (CFTR) protein using tryptic digestion. Wild-type and mutant ΔF508 CFTR were treated with ATP, AMP-PNP, and corrector VX-809 before limited tryptic digestion and analysis via western blotting. Data showed wild-type CFTR was stabilized by ATP and AMP-PNP binding, as indicated by stronger bands after digestion. ΔF508 CFTR showed little stability change until adjusting the analysis to a potential half-length fragment, then treatments produced significant stabilization. Future studies could investigate additional correctors and ATP analogues, plus other mutations and in vivo analyses.
Using Pathway Studio in Neurodegenerative disease
Dr. Gabor Juhasz of ELTE University in Budapest discusses use of Pathway Studio in the study of neurodegenerative diseases such as Alzheimer’s Disease.
FASEBJ-2015-277350v1-Varisco
1) Cela1 protein levels increase 176-fold during lung development from embryonic day 15.5 through 8 weeks postnatal.
2) After pneumonectomy, Cela1 mRNA increases 6-fold, protein increases 3-fold, and Cela1-positive cells increase 2-fold, indicating Cela1 is involved in compensatory lung growth.
3) Cela1 is expressed predominantly in alveolar type II cells during embryonic lung development and predominantly in CD90-positive fibroblasts in the postnatal lung.
Host modulation
This document discusses host modulation therapies for the treatment of periodontal disease. It first introduces concepts of host modulation and describes how therapies aim to downregulate destructive aspects of the host inflammatory response. A major focus is on matrix metalloproteinases (MMPs), which degrade connective tissue during periodontal disease. The document outlines various stages of MMP inhibition that could be targeted therapeutically, including inhibiting MMP induction, activation of latent MMPs, and upregulating natural MMP inhibitors. It provides extensive details on the mechanisms and clinical studies of sub-antimicrobial doses of doxycycline, one of the few approved host modulation drugs for treating periodontitis.
The use of repiderma in the healing of dehorning wounds 07 10 2013
This document discusses the use of Intra Repiderma spray for treating wounds from dehorning cattle. It finds that Intra Repiderma, which contains copper and zinc chelates, healed dehorning wounds faster than an antibiotic spray in a study of 20 calves. The copper has antimicrobial properties while both copper and zinc promote wound healing. No adverse effects or infections occurred with Intra Repiderma treatment. It provides a good alternative to antibiotic sprays for disinfecting and accelerating the healing of dehorning wounds.
Mechanisms of periodontal disease production
Bacteria are the primary cause of periodontal disease. To cause damage, bacteria must colonize the gingival crevice, damage the epithelial barrier, and produce substances that cause tissue damage. Key pathogens like Porphyromonas gingivalis produce proteases and other enzymes that degrade collagen and bone, as well as evade host defenses. The immune response also contributes to tissue degradation through the release of cytokines and enzymes from cells like neutrophils and macrophages. Osteoclasts are the main bone resorbing cells, and are activated by factors from bacteria and host tissues to break down bone.
14. wound healing (65) Dr Rahul Tiwari OMFS SIBAR Institute of Dental Science...
14. wound healing (65) Dr Rahul Tiwari OMFS SIBAR Institute of Dental Science...CLOVE Dental OMNI Hospitals Andhra Hospital
This document discusses wound healing and fracture healing processes. It describes the phases of wound healing including inflammation, granulation tissue formation, and wound contraction in primary and secondary healing. Factors influencing wound healing like infection, nutrition and diabetes are covered. Complications of healing such as hypertrophic scarring and keloid formation are mentioned. Commonly used wound healing materials like oxidized regenerated cellulose and Whitehead's varnish are briefly described. Fracture healing through procallus formation, osseous callus formation and remodeling is summarized.More Related Content
What's hot
ECCLU 2011 - N. Clarke - Bone targeted agents
1. The document discusses bone metabolism and prostate cancer, describing how factors like RANK ligand and osteoprotegerin regulate the balance between bone formation and resorption.
2. It summarizes a clinical trial comparing denosumab, a RANK ligand inhibitor, to zoledronic acid for treating bone metastases in prostate cancer patients. Denosumab delayed time to first skeletal-related event compared to zoledronic acid and reduced risk of multiple events.
3. Rates of adverse events were generally similar between the treatments, though denosumab resulted in fewer acute phase reactions and more cases of osteonecrosis of the jaw compared to zoledronic acid.
Extracellular vesicles from human liver stem cells restore arginosuccinate sy...
This study investigated using extracellular vesicles (EVs) derived from normal human liver stem cells (HLSCs) to treat argininosuccinate synthase deficiency (ASS1), which causes citrullinemia type I. The study found that:
1) EVs from HLSCs contained wild-type ASS1 protein, mRNA, and DNA.
2) When EVs from normal HLSCs were applied to ASS1-deficient hepatocytes differentiated from patients' HLSCs, they increased urea production, ASS1 enzymatic activity, and decreased DNA mutations in the recipient cells.
3) This suggests EVs derived from stem cells have potential to correct ASS1 enzyme deficiencies by transferring functional proteins and
Effect Of Ethanol In Oligodendrocytes, research miami
This study aimed to determine if ethanol's protective effects against oxidative stress in injured organisms is due to increasing cAMP signaling pathways. The researchers hypothesized that treating cells with ethanol after injury would increase cAMP, PKA, and CREB production, decreasing cell death. Their methods involved inducing spinal cord injuries in rats, culturing cells from the spinal cord and brain, treating cells with ethanol and hydrogen peroxide, and analyzing PKA and CREB levels and cell apoptosis. Their results showed that treating cells with 5% ethanol for 24 hours after oxidative stress increased PKA and CREB levels and reduced cell death. They concluded that combining a 5% ethanol treatment for 24 hours with a pharmacological agent could be a functional treatment to reduce paralysis
Following the Evolution of New Protein Folds via Protodomains
Protein evolution proceeds through genetic mechanisms, but selection acts on biological assemblies. I define a protodomain as a minimal independently evolving unit with conserved structure. Protodomain rearrangements have minimal impact on biological assemblies, so they represent a valid evolutionary path through fold space.
These slides are from my Candidacy Exam on Jan 28, 2013 at University of California, San Diego. It discusses my current research in Philip Bourne's lab, as well as proposes research for my thesis over the next two years. An audio version is available at http://www.scivee.tv/node/57082
ASBMB_Poster_3_25_2015_Jake
This document summarizes research analyzing relationships between lysine ubiquitylation and acetylation sites from proteomic datasets in Arabidopsis thaliana proteins. The research compared ubiquitylation and acetylation sites identified in previous publications and found that 9 non-nuclear proteins experienced both post-translational modifications. Most of these proteins were located in energy-associated organelles. While this provides support for potential cross-talk between ubiquitylation and acetylation, more acetylation and ubiquitylation data is still needed to further investigate these relationships and whether acetylation directly or indirectly influences ubiquitylation.
ASBMB_Poster_3_25_2015_Jake
This document summarizes research analyzing relationships between lysine ubiquitylation and acetylation sites in Arabidopsis thaliana proteins from proteomic datasets. The researchers compared ubiquitylation and acetylation sites identified in previous publications and found that 9 proteins experienced both post-translational modifications. Most of these proteins were located in energy-associated organelles, supporting the hypothesis that ubiquitylation and acetylation interactions could influence metabolic pathways. Future work is needed to identify more acetylation and ubiquitylation sites to better understand potential direct or indirect influences between the two modifications.
MSBMB 2016 conference poster
The document describes cloning, expressing, and purifying a glutathione S-transferase (GST) from Pseudomonas putida KT2440 and investigating its potential role in iron metal bioremediation. Key findings include:
- The GST gene (PP_2933) was cloned, expressed producing a 31kDa protein, and purified using ion exchange chromatography.
- The purified GST showed activity with certain substrates, including ethacrynic acid and cumene peroxide, indicating a role in oxidative stress response.
- Cells expressing the recombinant GST showed increased resistance to iron, suggesting it may bind iron complexes and aid in bioremediation of iron toxicity.
JBC1
This study investigated the degradation of soluble and myofibrillar proteins in rabbit muscle extracts by the ATP-ubiquitin-proteasome pathway. The key findings were:
1) Degradation of soluble muscle proteins was stimulated up to 6-fold by ATP and inhibited by removal of proteasomes or addition of proteasome inhibitors, indicating these proteins are substrates of the ATP-ubiquitin-proteasome pathway.
2) Purified myofibrillar proteins (myosin, actin, troponin, tropomyosin) added to the extracts were also degraded in an ATP-dependent manner, showing they can serve as substrates of this pathway.
3)
DEREKCV1216most current
This document provides biographical and professional details about Derek J. Milner, including his education, research experience, teaching experience, awards, publications, and presentations. It outlines his educational background, including a Ph.D. in Molecular and Cellular Biology from Baylor College of Medicine and a B.S. in Biochemistry from the University of Iowa. It then details his extensive research experience in regenerative biology and tissue engineering at the University of Illinois over the past 15 years, focusing on projects involving muscle regeneration, stem cells, and animal models of bone and cartilage repair.
PNAS
1) Rates of ubiquitin conjugation (attachment of ubiquitin molecules) to proteins increase in muscles undergoing atrophy (wasting away), largely due to activation of the N-end rule pathway.
2) The N-end rule pathway is responsible for most changes in ubiquitin conjugation rates in atrophying muscles from tumor-bearing or septic rats. Inhibitors of this pathway suppressed the increased ubiquitin conjugation seen in these conditions.
3) Ubiquitin conjugation rates increase in atrophying conditions like cancer cachexia and sepsis but decrease in hypothyroid muscles, and this response is mediated by the N-end rule pathway.
ATP Review
ATP (adenosine triphosphate) is the molecular unit that stores and transports energy within cells. It fuels enzymes and is present in all cells, providing energy for physiological processes. ATP is continuously recycled in the body, turning over its own body weight equivalent daily. Several studies have found that ATP supplementation can help reduce muscle fatigue, improve force output during exercise, increase muscle mass, strength and power, and provide small ergogenic effects on muscular strength under some conditions. However, more research is needed before recommending ATP supplementation to athletes seeking ergogenic aids.
poster final
This study aimed to validate an osteocyte-specific growth hormone receptor (GHR) knockout mouse model generated using the Cre/loxP system with Cre recombinase driven by the dentin matrix protein 1 (DMP1) promoter. The researchers found that while the DMP1-derived Cre mediated GHR knockout specifically in osteocytes as intended, GHR gene recombination was also detected in muscle tissue. They concluded that the DMP1-GHRKO mouse model is valid for studying the role of GHR in osteocytes, but the bone phenotype needs to be characterized with the knowledge that the gene recombination also occurred in muscle.
Smith_CV_Full
Austin E. Smith is a PhD candidate in chemistry at UNC-Chapel Hill studying protein amide-hydrogen exchange in vitro and in living cells using NMR. He received his BS from Furman University and his MS from Furman, studying inhibition of HMGA1 proteins by distamycin A using NMR and fluorescence spectroscopy. His research experience includes protein expression, purification, calorimetry, fluorescence, and molecular biology techniques. He has mentored undergraduates, post-bacs, and graduate students in his lab.
Hum. Mol. Genet.-2014-Goldstein-hmg-ddu390
1) Muscle injury in mice lacking the protein γ-sarcoglycan causes an increase in SMAD signaling, which contributes to transforming growth factor β (TGFβ) signaling.
2) Reducing SMAD signaling through introducing a heterozygous mutation of the gene SMAD4 in γ-sarcoglycan mice led to improved body mass, cardiac function, and muscle force compared to γ-sarcoglycan mice.
3) While fibrosis was not reduced, membrane damage was decreased, suggesting intracellular SMAD4 targets are important for mediating muscle and heart dysfunction in muscular dystrophy.
Osteoimmunology
This document discusses osteoimmunology, which examines the interactions between the bone and immune systems. It notes that bone cells and immune cells share a common origin and influence each other bidirectionally. Specifically, it outlines that osteoblasts regulate the hematopoietic stem cell niche that gives rise to immune cells, while osteoclasts are related to macrophages and dendritic cells. Additionally, cytokines and other signaling molecules regulate both bone and immune cell activity. A key example is RANKL, produced by osteocytes, which contributes to bone loss and influences lymphocyte development by activating RANK on osteoclast progenitors.
SCE Presentation
This study investigated the structural stability of the cystic fibrosis transmembrane conductance regulator (CFTR) protein using tryptic digestion. Wild-type and mutant ΔF508 CFTR were treated with ATP, AMP-PNP, and corrector VX-809 before limited tryptic digestion and analysis via western blotting. Data showed wild-type CFTR was stabilized by ATP and AMP-PNP binding, as indicated by stronger bands after digestion. ΔF508 CFTR showed little stability change until adjusting the analysis to a potential half-length fragment, then treatments produced significant stabilization. Future studies could investigate additional correctors and ATP analogues, plus other mutations and in vivo analyses.
Using Pathway Studio in Neurodegenerative disease
Dr. Gabor Juhasz of ELTE University in Budapest discusses use of Pathway Studio in the study of neurodegenerative diseases such as Alzheimer’s Disease.
FASEBJ-2015-277350v1-Varisco
1) Cela1 protein levels increase 176-fold during lung development from embryonic day 15.5 through 8 weeks postnatal.
2) After pneumonectomy, Cela1 mRNA increases 6-fold, protein increases 3-fold, and Cela1-positive cells increase 2-fold, indicating Cela1 is involved in compensatory lung growth.
3) Cela1 is expressed predominantly in alveolar type II cells during embryonic lung development and predominantly in CD90-positive fibroblasts in the postnatal lung.
What's hot (18)
Extracellular vesicles from human liver stem cells restore arginosuccinate sy...
Extracellular vesicles from human liver stem cells restore arginosuccinate sy...
Effect Of Ethanol In Oligodendrocytes, research miami
Effect Of Ethanol In Oligodendrocytes, research miami
Following the Evolution of New Protein Folds via Protodomains
Following the Evolution of New Protein Folds via Protodomains
Viewers also liked
Host modulation
This document discusses host modulation therapies for the treatment of periodontal disease. It first introduces concepts of host modulation and describes how therapies aim to downregulate destructive aspects of the host inflammatory response. A major focus is on matrix metalloproteinases (MMPs), which degrade connective tissue during periodontal disease. The document outlines various stages of MMP inhibition that could be targeted therapeutically, including inhibiting MMP induction, activation of latent MMPs, and upregulating natural MMP inhibitors. It provides extensive details on the mechanisms and clinical studies of sub-antimicrobial doses of doxycycline, one of the few approved host modulation drugs for treating periodontitis.
The use of repiderma in the healing of dehorning wounds 07 10 2013
This document discusses the use of Intra Repiderma spray for treating wounds from dehorning cattle. It finds that Intra Repiderma, which contains copper and zinc chelates, healed dehorning wounds faster than an antibiotic spray in a study of 20 calves. The copper has antimicrobial properties while both copper and zinc promote wound healing. No adverse effects or infections occurred with Intra Repiderma treatment. It provides a good alternative to antibiotic sprays for disinfecting and accelerating the healing of dehorning wounds.
Mechanisms of periodontal disease production
Bacteria are the primary cause of periodontal disease. To cause damage, bacteria must colonize the gingival crevice, damage the epithelial barrier, and produce substances that cause tissue damage. Key pathogens like Porphyromonas gingivalis produce proteases and other enzymes that degrade collagen and bone, as well as evade host defenses. The immune response also contributes to tissue degradation through the release of cytokines and enzymes from cells like neutrophils and macrophages. Osteoclasts are the main bone resorbing cells, and are activated by factors from bacteria and host tissues to break down bone.
14. wound healing (65) Dr Rahul Tiwari OMFS SIBAR Institute of Dental Science...
14. wound healing (65) Dr Rahul Tiwari OMFS SIBAR Institute of Dental Science...CLOVE Dental OMNI Hospitals Andhra Hospital
This document discusses wound healing and fracture healing processes. It describes the phases of wound healing including inflammation, granulation tissue formation, and wound contraction in primary and secondary healing. Factors influencing wound healing like infection, nutrition and diabetes are covered. Complications of healing such as hypertrophic scarring and keloid formation are mentioned. Commonly used wound healing materials like oxidized regenerated cellulose and Whitehead's varnish are briefly described. Fracture healing through procallus formation, osseous callus formation and remodeling is summarized.Wound healing
The document discusses factors that can affect wound healing by interfering with the normal phases of wound repair. It describes the four phases of wound healing and notes that interruptions or abnormalities in the phases can lead to delayed or impaired healing. Both local factors, like infection, oxygen levels, and venous insufficiency, as well as systemic factors, such as age, stress, diabetes, obesity, and certain medications, are reviewed as influences on wound healing. Proper wound healing requires coordination of the phases at the right times and intensities, and internal and external factors can disrupt this process.
Wound healing dr sumer
This document discusses the phases and cellular processes involved in wound healing. It describes three phases - inflammatory, proliferative, and maturation. The inflammatory phase involves hemostasis, recruitment of inflammatory cells, and production of growth factors. The proliferative phase involves angiogenesis, fibroplasia, and re-epithelialization to form granulation tissue. The maturation phase involves remodeling of scar tissue and collagen. Local and systemic factors that can impact wound healing are also discussed.
wound healing PPT
Wound healing involves three phases: inflammatory, proliferative, and maturational. Primary healing occurs when wound edges are in direct contact while secondary healing involves granulation tissue formation. Many factors can affect wound healing including infection, nutrition, steroids, ischemia, and diabetes. Growth factors are important for different stages of healing. Stem cells can help regenerate damaged tissue. Aberrations like keloids and hypertrophic scarring exist. New treatments include skin substitutes, dressings, growth factors, and therapies like hyperbaric oxygen and offloading casts.
Viewers also liked (7)
The use of repiderma in the healing of dehorning wounds 07 10 2013
The use of repiderma in the healing of dehorning wounds 07 10 2013
14. wound healing (65) Dr Rahul Tiwari OMFS SIBAR Institute of Dental Science...
14. wound healing (65) Dr Rahul Tiwari OMFS SIBAR Institute of Dental Science...
Similar to MMP's and the Role of Zinc in Wound Healing
Pharmacogenomic of TPMT which affected to plasma level of thiopurine drugs
This document outlines a presentation on thiopurine drugs including azathioprine, 6-mercaptopurine, and 6-thioguanine. It discusses their metabolism, mechanisms of action, adverse drug reactions, and importance of testing for thiopurine S-methyltransferase (TPMT) polymorphisms. TPMT activity affects levels of active drug metabolites and risk of toxicity. Genotype correlates with but does not perfectly predict phenotype. The presentation emphasizes dose adjustment based on TPMT levels and close monitoring to improve outcomes with thiopurine treatment.
2013 SOT_NAMPT_CFU_poster
1. Two NAMPTi, GMX-1778 and APO-866, reduced colony formation and ATP levels of human megakaryocytes in vitro, indicating toxicity to platelet precursors. This manifested as thrombocytopenia in clinical trials.
2. Nicotinamide co-treatment mitigated the toxicity of NAMPTi on megakaryocytes in vitro and prevented bone marrow hypocellularity in rats treated with NAMPTi.
3. The results suggest NAMPTi toxicity targets megakaryocytes through inhibition of NAD production during differentiation. Co-treatment with nicotinamide may protect megakaryocytes and improve the safety profile of NAMPTi.
Nanotechnology and its Application in Cancer Treatment
Nanotechnology
Nanomaterials
Nanostructures
Nanoparticles
Unexpected Optical Properties of Nanoparticles
Synthesis of Nanoparticles
Nanotechnology in Cancer Treatment
Role of Sulfur NPs in Cancer Treatment
Human Tumour Cell Lines Used in Research
Ehrlich ascites carcinoma (EAC)
Sulfur Nanoparticles Preparation
MTT Assay
Sulphorhodamine-B (SRB) Assay
Median lethal dose (LD 50)
Experimental design
FT-IR Characterization of Sulfur Nanoparticles
SEM Characterization of Sulfur Nanoparticles
EDS Characterization of Sulfur Nanoparticles
XRD Characterization of Sulfur Nanoparticles
Chemical Studies on Sulfur Nanoparticles In Vitro
Biochemical investigations
Conclusion
Applications of Nanoparticles in cancer treatment
Nanoshells
Nano X-Ray therapy
Drug Delivery by Nanoparticles
Mineral Metabolism
Minerals are essential for normal growth and maintenance of the body.
Major elements : Requirement >100 mg /day
Trace Elements : Requirement <100mg/day
Some are necessary for the body but their exact functions are not known.
Ex.: Chromium, Nickel, Bromide, Lithium, Barium
Non-Essentials : seen in tissues. Contaminants in food stuffs.
Ex.: Rubedium, Silver, Gold, Bismuth
Toxic : should be avoided.
Ex.: Aluminium, Lead, Cadmium, Mercury
Models of Human Diseases Conference (2010) Tetrahymena model by Dr. R. Pearl...
Models of Human Diseases Conference (2010) Tetrahymena model by Dr. R. Pearl...Medical Education Advising
The Ciliate Protozoan Tetrahymena thermophila as an important animal model organism
Dr. R.E. Pearlman, York University
Models of Human Diseases Conference
June 29, 2010Radiosenitizers and radioprotectors
It is a summed up discussion about various radiosensitizers and radioprotectors, which are presently in use or was used previously.
Anticancer drugs - drdhriti
The document discusses various types of anticancer drugs including alkylating agents like cisplatin and cyclophosphamide, antimetabolites like methotrexate and 5-fluorouracil, and targeted drugs. It describes their mechanisms of action, toxicities, and common uses in treating cancers like breast cancer, leukemia, lymphoma, and others. The goal of cancer chemotherapy is to cure cancer when possible or induce remission, but the drugs can also be used for palliation to reduce tumor size and prolong life when the cancer is not curable.
Non-Invasive Methods for Biomonitoring Trace Element Exposure
Finger and toe nails don't seem very interesting at the best of times, but did you know they carry a record of your exposure to metals like lead and arsenic?
In this presentation I review how these materials can show if people are being exposed to trace metals and how they can be used to link exposure to possible early health effects.
The talk includes work from 3 PhD projects that I have been involved in and show how a project can develop from a humble beginning to cutting edge research.
Mechanism of protection by daily...
This document describes cyclophosphamide (CP) chemotherapy and its toxic side effects, particularly on the testes. CP requires hepatic metabolism to exert toxicity. Diallyl disulfide (DADS), a garlic compound, is a potent antioxidant that may protect against toxicity. The study aimed to evaluate whether DADS protects against CP-induced testicular toxicity in rats. Rats received DADS, CP, or both over 10 days. CP decreased weight gain and food intake while DADS prevented these effects. CP reduced reproductive organ weights and sperm quality, while DADS attenuated these toxicities. DADS may protect the testes by modulating hepatic CP metabolism and reducing oxidative stress.
Super Males: Antioxidants boost male fertility and sperm viability in fruit f...
Antioxidants may improve male fertility in Drosophila melanogaster. The study investigated the effects of melatonin and lipoic acid antioxidants on sperm viability, mating behavior, and offspring production. Males fed antioxidants had higher sperm viability, increased mating rates, and produced more offspring than control-fed males. The antioxidants likely protected sperm from oxidative damage induced by paraquat, demonstrating their potential to boost male fertility.
Metastatic bone disease: An old dogma and a new insight
Metastatic bone disease is a challenging condition that places a heavy burden on patients. New insights into the cellular and molecular mechanisms have led to improved treatments. Cancer cells interact with the bone microenvironment through factors like RANKL, RANK, and osteoprotegerin, inducing a "vicious cycle" of bone destruction. Emerging therapies target these interactions by inhibiting RANKL with drugs like denosumab. Radiopharmaceuticals like radium-223 also show promise by targeting areas of new bone growth in metastases. While radiation remains important for pain relief, combination therapies offer the potential for improved outcomes in metastatic bone disease.
Antioxidants and Fertility in The Common Fruit Fly
Antioxidants may improve male fertility in Drosophila melanogaster. The study investigated the effects of melatonin and lipoic acid antioxidants on mating behavior, sperm viability, and fertility when males were exposed to the oxidative stress of paraquat. Males fed antioxidants had higher mating rates, sperm viability over 70%, and produced more offspring than controls fed ethanol. The results suggest antioxidants protect sperm from oxidative damage and help maintain male fertility under stress.
Presentazione prodotto
The document discusses the health benefits of mangosteen, a fruit native to Thailand. It notes that mangosteen has been traditionally used in Asian medicine for centuries and contains powerful antioxidants called xanthones. Studies show xanthones in mangosteen have anti-inflammatory, anticancer and antimicrobial properties and can help treat conditions like heart disease, diabetes, Alzheimer's, and skin issues like acne. The document promotes Xango, a company that produces mangosteen supplements, and lists many ways mangosteen may benefit health.
Bone Health in Prostate Cancer Patients.
It describes the pathophysiology, molecular biology and management of bone related problem among prostate cancer patients.
Pathophysiology: Myasthenia Gravis & Restless Leg Syndrome
This presentation was given to first year pharmacy students as a part of course on medical physiology and pathophysiology.
Anthracycline seminar
This presentation describes about discovery mechanism of action, metabolism, mechanism of resistance and pharmacokinetics of anthracyclines
marine anticancer drugs'.pptx
Marine sources provide a rich diversity of chemical compounds with potential pharmaceutical applications including anticancer drugs. Compounds from marine flora such as microbes, algae, and cyanobacteria have shown cytotoxic, antitumor and antiproliferative effects against various cancer cell lines. Two notable marine-derived anticancer drugs are ecteinascidin-743 from the Caribbean tunicate and cytarabine from the Caribbean sponge. Ecteinascidin-743 is a first-in-class alkylating agent that covalently binds DNA. Cytarabine is a nucleoside analog that inhibits DNA synthesis. Both drugs demonstrate activity against hematological cancers and have been incorporated into standard chemotherapy regimens.
Systematic screening of generic drugs for progressive multiple sclerosis iden...
This document describes experiments investigating the effects of clomipramine in models of multiple sclerosis. Clomipramine showed neuroprotective effects against iron-mediated toxicity in neurons in culture. It also demonstrated antioxidant properties and reduced T-lymphocyte and B-lymphocyte proliferation. In mouse models of EAE, clomipramine decreased disease burden and inflammation when administered from disease onset. Further experiments explored the effects of clomipramine in various EAE models induced with different antigens. Overall, the results suggest clomipramine has therapeutic potential for reducing inflammation and neurodegeneration in multiple sclerosis.
Sodium Thiosulfate (Hydrogen Sulfide Donor): Ameliorates the Pituitary-testic...
Sodium Thiosulfate (Hydrogen Sulfide Donor): Ameliorates the Pituitary-testic...BRNSSPublicationHubI
This study investigated the protective effects of sodium thiosulfate (STS) on the pituitary-testicular axis dysfunction caused by cyclophosphamide (CYP) and/or ionizing gamma radiation (IR) in rats. Rats received STS before and during treatment with CYP and/or IR, while control groups received CYP and/or IR only. STS significantly reduced oxidative stress in the pituitary gland and testes by lowering malondialdehyde and increasing antioxidant enzyme activities. It also elevated reduced luteinizing hormone, follicle-stimulating hormone, and testosterone levels. Furthermore, STS reduced pathological changes and apoptosis in the pituitary and testes induced by CYP and/or IR. This study demonstratesCompleteThesis_ChristianeRiedinger.pdf
A DPhil Thesis completed at Christ Church College, University of Oxford, Department of Biochemistry.
Similar to MMP's and the Role of Zinc in Wound Healing (20)
Pharmacogenomic of TPMT which affected to plasma level of thiopurine drugs
Pharmacogenomic of TPMT which affected to plasma level of thiopurine drugs
Nanotechnology and its Application in Cancer Treatment
Nanotechnology and its Application in Cancer Treatment
Models of Human Diseases Conference (2010) Tetrahymena model by Dr. R. Pearl...
Models of Human Diseases Conference (2010) Tetrahymena model by Dr. R. Pearl...
Non-Invasive Methods for Biomonitoring Trace Element Exposure
Non-Invasive Methods for Biomonitoring Trace Element Exposure
Super Males: Antioxidants boost male fertility and sperm viability in fruit f...
Super Males: Antioxidants boost male fertility and sperm viability in fruit f...
Metastatic bone disease: An old dogma and a new insight
Metastatic bone disease: An old dogma and a new insight
Antioxidants and Fertility in The Common Fruit Fly
Antioxidants and Fertility in The Common Fruit Fly
Pathophysiology: Myasthenia Gravis & Restless Leg Syndrome
Pathophysiology: Myasthenia Gravis & Restless Leg Syndrome
Systematic screening of generic drugs for progressive multiple sclerosis iden...
Systematic screening of generic drugs for progressive multiple sclerosis iden...
Sodium Thiosulfate (Hydrogen Sulfide Donor): Ameliorates the Pituitary-testic...
Sodium Thiosulfate (Hydrogen Sulfide Donor): Ameliorates the Pituitary-testic...
Recently uploaded
Post-Menstrual Smell- When to Suspect Vaginitis.pptx
Do you have abnormal smell after periods? It may be vaginitis. Do not worry, herbal medicine Fuyan Pill can help you get a cure.
LOOPS in orthodontics t loop bull loop vertical loop mushroom loop stop loop
vertical loop
horizontal loop
Hemodialysis: Chapter 4, Dialysate Circuit - Dr.Gawad
- Video recording of this lecture in English language: https://youtu.be/kqbnxVAZs-0
- Video recording of this lecture in Arabic language: https://youtu.be/SINlygW1Mpc
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Top-Vitamin-Supplement-Brands-in-India List
Swisschem Dermacare provides the Top 10 Vitamin Supplement Brands in India. To know more about us give us call at our official number
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
The Electrocardiogram - Physiologic Principles
These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
vonoprazan A novel drug for GERD presentation
Vonoprazan, a new potassium acid channel blocker used in the conditions of GERD and gastric ulcer, duodenal ulcer ....
Histopathology of Rheumatoid Arthritis: Visual treat
Histopathology of Rheumatoid Arthritis: Visual treat
TEST BANK For Community Health Nursing A Canadian Perspective, 5th Edition by...
TEST BANK For Community Health Nursing A Canadian Perspective, 5th Edition by Stamler, Verified Chapters 1 - 33, Complete Newest Version Community Health Nursing A Canadian Perspective, 5th Edition by Stamler, Verified Chapters 1 - 33, Complete Newest Version Community Health Nursing A Canadian Perspective, 5th Edition by Stamler Community Health Nursing A Canadian Perspective, 5th Edition TEST BANK by Stamler Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Pdf Chapters Download Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Pdf Download Stuvia Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Study Guide Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Ebook Download Stuvia Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Questions and Answers Quizlet Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Studocu Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Quizlet Test Bank For Community Health Nursing A Canadian Perspective, 5th Edition Stuvia Community Health Nursing A Canadian Perspective, 5th Edition Pdf Chapters Download Community Health Nursing A Canadian Perspective, 5th Edition Pdf Download Course Hero Community Health Nursing A Canadian Perspective, 5th Edition Answers Quizlet Community Health Nursing A Canadian Perspective, 5th Edition Ebook Download Course hero Community Health Nursing A Canadian Perspective, 5th Edition Questions and Answers Community Health Nursing A Canadian Perspective, 5th Edition Studocu Community Health Nursing A Canadian Perspective, 5th Edition Quizlet Community Health Nursing A Canadian Perspective, 5th Edition Stuvia Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Pdf Chapters Download Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Pdf Download Stuvia Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Study Guide Questions and Answers Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Ebook Download Stuvia Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Questions Quizlet Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Studocu Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Quizlet Community Health Nursing A Canadian Perspective, 5th Edition Test Bank Stuvia
Does Over-Masturbation Contribute to Chronic Prostatitis.pptx
In some case, your chronic prostatitis may be related to over-masturbation. Generally, natural medicine Diuretic and Anti-inflammatory Pill can help mee get a cure.
TEST BANK For Community and Public Health Nursing: Evidence for Practice, 3rd...
TEST BANK For Community and Public Health Nursing: Evidence for Practice, 3rd Edition by DeMarco, Walsh, Verified Chapters 1 - 25, Complete Newest Version TEST BANK For Community and Public Health Nursing: Evidence for Practice, 3rd Edition by DeMarco, Walsh, Verified Chapters 1 - 25, Complete Newest Version TEST BANK For Community and Public Health Nursing: Evidence for Practice, 3rd Edition by DeMarco, Walsh, Verified Chapters 1 - 25, Complete Newest Version Test Bank For Community and Public Health Nursing: Evidence for Practice 3rd Edition Pdf Chapters Download Test Bank For Community and Public Health Nursing: Evidence for Practice 3rd Edition Pdf Download Stuvia Test Bank For Community and Public Health Nursing: Evidence for Practice 3rd Edition Study Guide Test Bank For Community and Public Health Nursing: Evidence for Practice 3rd Edition Ebook Download Stuvia Test Bank For Community and Public Health Nursing: Evidence for Practice 3rd Edition Questions and Answers Quizlet Test Bank For Community and Public Health Nursing: Evidence for Practice 3rd Edition Studocu Test Bank For Community and Public Health Nursing: Evidence for Practice 3rd Edition Quizlet Test Bank For Community and Public Health Nursing: Evidence for Practice 3rd Edition Stuvia Community and Public Health Nursing: Evidence for Practice 3rd Edition Pdf Chapters Download Community and Public Health Nursing: Evidence for Practice 3rd Edition Pdf Download Course Hero Community and Public Health Nursing: Evidence for Practice 3rd Edition Answers Quizlet Community and Public Health Nursing: Evidence for Practice 3rd Edition Ebook Download Course hero Community and Public Health Nursing: Evidence for Practice 3rd Edition Questions and Answers Community and Public Health Nursing: Evidence for Practice 3rd Edition Studocu Community and Public Health Nursing: Evidence for Practice 3rd Edition Quizlet Community and Public Health Nursing: Evidence for Practice 3rd Edition Stuvia Community and Public Health Nursing: Evidence for Practice 3rd Edition Test Bank Pdf Chapters Download Community and Public Health Nursing: Evidence for Practice 3rd Edition Test Bank Pdf Download Stuvia Community and Public Health Nursing: Evidence for Practice 3rd Edition Test Bank Study Guide Questions and Answers Community and Public Health Nursing: Evidence for Practice 3rd Edition Test Bank Ebook Download Stuvia Community and Public Health Nursing: Evidence for Practice 3rd Edition Test Bank Questions Quizlet Community and Public Health Nursing: Evidence for Practice 3rd Edition Test Bank Studocu Community and Public Health Nursing: Evidence for Practice 3rd Edition Test Bank Quizlet Community and Public Health Nursing: Evidence for Practice 3rd Edition Test Bank Stuvia
Journal Article Review on Rasamanikya
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
It includes regulation of combination products and medical devices. FDA and industry liaisons.
Ketone bodies and metabolism-biochemistry
This slide consists of all the topics of ketone . This can be used for exam purpose for writing about Diabetic keto acidosis etc . Thank you
Recently uploaded (20)
Post-Menstrual Smell- When to Suspect Vaginitis.pptx
Post-Menstrual Smell- When to Suspect Vaginitis.pptx
LOOPS in orthodontics t loop bull loop vertical loop mushroom loop stop loop
LOOPS in orthodontics t loop bull loop vertical loop mushroom loop stop loop
Hemodialysis: Chapter 4, Dialysate Circuit - Dr.Gawad
Hemodialysis: Chapter 4, Dialysate Circuit - Dr.Gawad
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Histopathology of Rheumatoid Arthritis: Visual treat
Histopathology of Rheumatoid Arthritis: Visual treat
TEST BANK For Community Health Nursing A Canadian Perspective, 5th Edition by...
TEST BANK For Community Health Nursing A Canadian Perspective, 5th Edition by...
Does Over-Masturbation Contribute to Chronic Prostatitis.pptx
Does Over-Masturbation Contribute to Chronic Prostatitis.pptx
TEST BANK For Community and Public Health Nursing: Evidence for Practice, 3rd...
TEST BANK For Community and Public Health Nursing: Evidence for Practice, 3rd...
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
REGULATION FOR COMBINATION PRODUCTS AND MEDICAL DEVICES.pptx
Vestibulocochlear Nerve by Dr. Rabia Inam Gandapore.pptx
Vestibulocochlear Nerve by Dr. Rabia Inam Gandapore.pptx
Muscles of Mastication by Dr. Rabia Inam Gandapore.pptx
Muscles of Mastication by Dr. Rabia Inam Gandapore.pptx
MMP's and the Role of Zinc in Wound Healing
- 1. MMP’s and the Role of Zinc inWound Healing Dr. MitchellV. Kaminski, Jr. Board Certified in Nutrition, Surgery andWound Healing
- 2. Origin of Zinc – Crab Nebula supernova Zinc complete electron shell therefore powerful antioxidant Used by permission ofThe University ofTexas McDonald Observatory. Green – Hydrogen (H) Red – Nitrogen (N) Reddish - Sulfur (S) Blue – Oxygen (O) Violet – Helium (He) Brown – Polycyclic aromatic Hydrocarbons (C+) CHO = Carbohydrate & Fat CHON = Protein
- 3. Role of Zinc • Total Body Content 1500-3000mg • (IronTBC – 2300-3800mg) • 300+ Enzyme Reactions • Epidermal integrity & resistance • Antioxidant • Sex hormone, insulin & serotonin • EU Zincage Study • Chromosomal RNA/DNA transcription • Genome stability • Signal triggering • MMPs • Immunity – neutrophils, killer cells, macrophages
- 4. Determining a Zinc Deficiency •Serum zinc vs. functional test
- 5. ZincTasteTest (ZTT) •Zicam oral spray •Repulsive taste to healthy adult •Gustin
- 7. Zinc Deficiency •Genetic or acquired Acrodermatitis Enteropathica (Genetic) Acquired
- 8. ZincToxicity •U.S. Food and Nutrition Board – 40mg/day •Cancel treatment post 6-weeks •Our Protocol – 100mg/day (220mg of Zinc Sulfate)
- 10. Biofilm •Complex community of microorganisms •Characterized by high cell density •Extracellular polymer matrix •Physical barrier against antimicrobial •Facilitates adhesion (tissue & foreign bodies) •Exopolysaccharide
- 11. Necrotic Debris and Biofilm •MMP9 •Tenacious •Mechanical Removal
- 12. MMPs •MMPs manufactured by macrophlages and membrane bound •15 of 20 MMPs found in wounds – collenganese activity •MMP9 = DirtyWound •MMPs digest all proteins in ECS & basement membrane
- 13. Extracellular matrix • Basement membrane • Proteoglycans • Heparan sulfate • Chondroitin sulfate • Keratan sulfate • Non-proteoglycan polysaccharide • Hyaluronic acid • Fibers • Collagen • Elastin • Other • Fibronectin • Laminin
- 14. MMP Inhibitors •4 tissue families •TIMP-1,TIMP-2,TIMP-3,TIMP-4 •Common cysteine switch
- 15. Conclusion • Critical to wound healing • MMPs are zinc dependent • Serum level unreliable • Functional test – simple & reliable • Proposal: Recommended lower limits must be reevaluated • Current: 70 ug/dL • Proposed new level be consistent with recovery of normal ZTT