Mise-en-scene refers to everything presented to the viewer within the frame of a shot, including color, costume, props, sets/locations, blocking/performance, and lighting. The document discusses how each of these elements can be used to convey meaning and set mood. Color, for example, is used symbolically and connotations of red indicate danger while white suggests purity. Costumes can reveal social class, with ill-fitting clothes implying a lower class. Lighting establishes mood, with high key lighting feeling professional and low key lighting creating suspense through shadows.