The document presents a method development and validation for the quantitative estimation of ondansetron hydrochloride using high performance thin layer chromatography (HPTLC). An HPTLC method was developed using a methanol and triethylamine-glacial acetic acid mobile phase to separate ondansetron hydrochloride. The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection, limit of quantitation, ruggedness and robustness. The developed and validated HPTLC method was successfully applied for the estimation of ondansetron hydrochloride in bulk drug samples and tablet dosage forms.
This document provides an overview of analyzing drugs from hair samples for forensic analysis. It discusses how drugs become incorporated into hair, specimen collection procedures, stability of drugs in hair, effects of cosmetic treatments, hair digestion procedures, drug analysis methods including immunoassays, chromatography, and sectional analysis. Applications of hair analysis include drug facilitated crimes, verifying drug history, determining gestational drug exposure, and post-mortem toxicology. Two case studies are presented where hair analysis aided investigations.
This document provides an overview and comparison of various in-vitro methods used to measure antioxidant activity, including their advantages and disadvantages. It summarizes several common methods such as the Thin Layer Chromatography autography technique, Cellular Antioxidant Activity assay, Dye-Substrate oxidation method, and Cupric Ion Reducing Antioxidant Capacity method. The document emphasizes selecting methods based on feasibility, simplicity, required instrumentation and ability to effectively analyze antioxidant properties.
Understanding the Analytical method validation in a Practical PerspectiveDr. Ishaq B Mohammed
The document discusses analytical method development, validation and transfer. It begins by introducing the importance of method development, validation and transfer in pharmaceutical analysis. It then discusses some key aspects of each including the objectives of method development, definition of validation, and the purpose of method transfer. The document provides examples of parameters to consider for method development including sample type, required data, analyte levels, and expected precision and accuracy. It also gives an overview of common validation parameters like accuracy, precision, specificity, range and linearity. The document aims to provide guidance on establishing reliable analytical methods for pharmaceutical applications.
This document provides information on an ELISA test kit for measuring androstenedione levels. It discusses the intended use of the kit for quantitative measurement of androstenedione in serum and plasma. It also summarizes the role and levels of androstenedione in males and females. The document describes the principles and procedures of the competitive binding ELISA test. It provides details on the reagents, materials, storage conditions, specimen collection and handling, assay procedure, calculation of results, and expected normal values.
This document provides information about anti-fungal susceptibility testing. It discusses various fungi and the available anti-fungal drugs that act on different fungal targets like the cell wall, cell membrane, microtubules, RNA/DNA, and protein synthesis. It covers different testing methods like macrodilution, microdilution, and disk diffusion. It provides details on test medium, inoculum preparation, drug dilutions, and incubation conditions. It also discusses interpretive criteria, quality control strains and ranges, emerging resistance, and the need for susceptibility testing.
The document presents a method development and validation for the quantitative estimation of ondansetron hydrochloride using high performance thin layer chromatography (HPTLC). An HPTLC method was developed using a methanol and triethylamine-glacial acetic acid mobile phase to separate ondansetron hydrochloride. The method was validated in terms of linearity, accuracy, precision, specificity, limit of detection, limit of quantitation, ruggedness and robustness. The developed and validated HPTLC method was successfully applied for the estimation of ondansetron hydrochloride in bulk drug samples and tablet dosage forms.
This document provides an overview of analyzing drugs from hair samples for forensic analysis. It discusses how drugs become incorporated into hair, specimen collection procedures, stability of drugs in hair, effects of cosmetic treatments, hair digestion procedures, drug analysis methods including immunoassays, chromatography, and sectional analysis. Applications of hair analysis include drug facilitated crimes, verifying drug history, determining gestational drug exposure, and post-mortem toxicology. Two case studies are presented where hair analysis aided investigations.
This document provides an overview and comparison of various in-vitro methods used to measure antioxidant activity, including their advantages and disadvantages. It summarizes several common methods such as the Thin Layer Chromatography autography technique, Cellular Antioxidant Activity assay, Dye-Substrate oxidation method, and Cupric Ion Reducing Antioxidant Capacity method. The document emphasizes selecting methods based on feasibility, simplicity, required instrumentation and ability to effectively analyze antioxidant properties.
Understanding the Analytical method validation in a Practical PerspectiveDr. Ishaq B Mohammed
The document discusses analytical method development, validation and transfer. It begins by introducing the importance of method development, validation and transfer in pharmaceutical analysis. It then discusses some key aspects of each including the objectives of method development, definition of validation, and the purpose of method transfer. The document provides examples of parameters to consider for method development including sample type, required data, analyte levels, and expected precision and accuracy. It also gives an overview of common validation parameters like accuracy, precision, specificity, range and linearity. The document aims to provide guidance on establishing reliable analytical methods for pharmaceutical applications.
This document provides information on an ELISA test kit for measuring androstenedione levels. It discusses the intended use of the kit for quantitative measurement of androstenedione in serum and plasma. It also summarizes the role and levels of androstenedione in males and females. The document describes the principles and procedures of the competitive binding ELISA test. It provides details on the reagents, materials, storage conditions, specimen collection and handling, assay procedure, calculation of results, and expected normal values.
This document provides information about anti-fungal susceptibility testing. It discusses various fungi and the available anti-fungal drugs that act on different fungal targets like the cell wall, cell membrane, microtubules, RNA/DNA, and protein synthesis. It covers different testing methods like macrodilution, microdilution, and disk diffusion. It provides details on test medium, inoculum preparation, drug dilutions, and incubation conditions. It also discusses interpretive criteria, quality control strains and ranges, emerging resistance, and the need for susceptibility testing.
This document provides an overview of preformulation studies for intramuscular injections. It discusses various parameters studied in preformulation including organoleptic properties, particle size, shape and distribution, powder flow properties, FTIR spectroscopy, DSC, X-ray diffraction, solubility, partition coefficient, and the effect of temperature on solubility. It also describes stability studies conducted at different stages including stress testing, accelerated and long-term testing to determine shelf life and storage conditions. The testing scope for liquid injectables includes parameters like pH, clarity, assay, degradation products, and functionality of container closure systems. Finally, it discusses climatic zones for stability testing and the hygroscopic nature of some drug substances.
Different Laboratory Equipment used in Toxicology and Molecular BiologyMuhammad Kamran (Sial)
This document describes various types of laboratory equipment used in toxicology and molecular biology. It discusses personal protective equipment, volume measuring tools like beakers and volumetric flasks, microscopes for identification, analyzers like microplate readers and electrophoresis apparatus, chromatography equipment, and other tools like refrigerators, sterilizers, and centrifuges. The functions, components, principles, and applications of these different pieces of equipment are explained.
The beautiful hair, how to grind hair sample for detectionsWISBIOMED
The document discusses guidelines for using human hair samples for drug testing. It provides details on:
- Properly collecting and storing hair samples, including recommended sampling locations, recording hair characteristics, defining root and tip sections, and minimizing degradation or contamination.
- Cleaning hair samples through solvent washes to remove external contaminants before analysis.
- Methods for disintegrating hair including grinding it into a powder using Precellys, an instrument that pulverizes samples through bead beating.
- Carrying out extraction and various drug tests on processed hair samples, including for opiates, cocaine, amphetamines, and cannabinoids.
High performance thin layer chromatography RevanHiwale1
This document describes a high performance thin layer chromatography (HPTLC) method for quantifying paracetamol, pseudoephedrine, and loratadine in pharmaceutical tablets and human plasma. The method involves separating the drugs on HPTLC plates using an acetone-hexane-ammonia mobile phase. Calibration curves are generated by plotting peak areas versus known concentrations of each drug. The method is then applied to analyze pharmaceutical tablets and human plasma samples spiked with the drugs. Results show the HPTLC method can successfully separate and quantify the three drugs simultaneously despite their complex concentration ratios in pharmaceutical formulations.
Those who administer ionizing radiation must become familiar with the magnitude of exposure encountered in medicine, dentistry and every day life; the possible risks associated with such exposure; and the methods used to affect exposure.
Practitioners should remain informed about safety updates to further improve diagnostic quality of radiographs and decrease radiation exposure.
External contamination of hair with heroin was evaluated in six volunteers over 3 months. All contaminated subjects tested positive for opiates like heroin, 6-MAM, morphine and acetylcodeine for at least 3 months after the contamination period. Significant levels of 6-MAM (>0.5 ng/mg) were detected until 6 weeks in all subjects. The 6-MAM/morphine ratio was always above 1.3, even 3 months after contamination. Decontamination procedures were not sufficient to remove drugs that had penetrated into hair from external contamination. This suggests a risk of false positives from external contamination when interpreting hair drug tests.
This document describes the methods for conducting an in vitro chromosomal aberration test. White blood cells are cultured from human donors and exposed to test substances like cyclophosphamide. Cells are harvested at different timepoints throughout the cell cycle. Slides are prepared, stained, and analyzed under a microscope to identify chromosomal abnormalities like deletions, duplications, inversions, and translocations. Acceptance criteria include having homogenous results between replicates, aberration frequencies within historical controls, and a concentration-dependent response to a test substance. A conclusion on the potential of a substance to induce chromosomal aberrations is based on statistical analyses.
Determination of Etodolac in Commercial Formulations by HPLC-UV Methodijtsrd
The aim of this study was to develop and verify a simple, rapid and sensitive high performance liquid chromatography method coupled with UV detector HPLC UV method for the quantitative determination of etodolac in bulk and pharmaceutical dosage forms. Chromatographic separation was performed at ambient conditions on a reverse phase ACE C8 analytical column 250 mm x 4.6 mm ID, 5 umm using the mobile phase containing acetonitrile water 80 20, v v at a flow rate of 1.0 mL min 1. A wavelength of 272 nm was used for etodolok and paracetamol IS . A retention time of 4.21 min and 2.02 min were obtained for etodolac and IS, respectively. The method showed linearity in the range of 0.08 10 µg mL 1 for etodolac R = 0.9999 . The linear regression equations obtained by least square regression method were the ratio of peak area of etodolac and IS =1.559 concentration etodolac µg mL 0.139. The intra day and inter day RE and RSD values of the method were =10.0 and =2.65 , respectively. Limit of detection LOD and limit of quantification LOQ were found to be 0.04 and 0.06 µg mL 1 for etodolac, respectively. A new, simple and sensitive high performance liquid chromatography method was developed and validated for etodolac. The method can be applied for the quantification of etodolac without derivatization in bulk solutions and commercial formulations using the internal standard. Tugrul Cagri Akman | Yucel Kadioglu "Determination of Etodolac in Commercial Formulations by HPLC-UV Method" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29452.pdfPaper URL: https://www.ijtsrd.com/pharmacy/analytical-chemistry/29452/determination-of-etodolac-in-commercial-formulations-by-hplc-uv-method/tugrul-cagri-akman
UV Spectrophotometeric Analytical Method Development and Validation for the D...ijtsrd
An easy, simple, specific, speedy, precise and accurate have been developed and validated for content determination of Telmisartan. This article based on validation of analytical method procedures which is established in ICH Q2 R1 .Telmisartan demonstrated the absorption maxima in at 291.2 nm and found was linear for a range of 5 µg ml ”“25 µg ml with correlation coefficient LOD of Telmisartan was found to be 2.09µg ml and the limit of quantification LOQ of Telmisartan was found to be 6.34 µg ml. The analytical method validation of the above proposed method was performed by carrying out precision and accuracy studies. The Accuracy percentage recovery on three different levels i.e. 25 , 50 and 75 was found to be 95.20 , 94.21 and 90.95 respectively. The proposed analytical method demonstrated good Intra precision Repeatability with relative standard deviations 2.54. The proposed analytical method was validated for the test parameter Specificity, Precision, Linearity and range, Ruggedness, Accuracy and recovery. The proposed method for content determination of Telmisartan in pureand tablet dosage form by UV spectrophotometer in pharmaceutical found easy, simple, accurate, precise and reproducible, economical and can be applied for the everyday quality control analysis. C. Shambiga | M. Menaka "UV Spectrophotometeric Analytical Method Development and Validation for the Determination of Telmisartan in Pharmaceutical Drug and Drug Formulation (Tablet Dosage Form)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-7 | Issue-3 , June 2023, URL: https://www.ijtsrd.com.com/papers/ijtsrd56293.pdf Paper URL: https://www.ijtsrd.com.com/pharmacy/other/56293/uv-spectrophotometeric-analytical-method-development-and-validation-for-the-determination-of-telmisartan-in-pharmaceutical-drug-and-drug-formulation-tablet-dosage-form/c-shambiga
This document provides instructions for using an ELISA kit to detect the mycotoxin zearalenone in cereal crops and animal feeds. It begins with an introduction to zearalenone and its health effects. It then describes the intended use, principle, reagents, materials, precautions, extraction procedure, and assay procedure for the zearalenone ELISA kit. The kit is designed to quantitatively detect zearalenone in samples through a competitive enzyme immunoassay.
Determinación por FISH de la deleción del cromosoma 7.pdfEduardoMasat1
This document provides troubleshooting recommendations for potential problems that may occur during fluorescence in situ hybridization (FISH). It lists several potential causes for issues like no FISH signals, weakening hybridization signals over time, diffuse signals, weak signals, and high background signals. Recommended solutions are provided for each potential cause, such as checking the microscope optics, adjusting temperatures and concentrations, and ensuring proper sample preparation. Customer support contact information is also included.
PureLizz CST-50 A Revolutionary System for Hair Reshaping developed by Pure K...Daniel Marks
PureLizz CST-50 is the safest and most effective way to change the structural hair fiber into either curly or straight, or as desired by the professional hairdresser.
PureLizz System is a unique and safe process, promoting the cleavage of the keratin disulfide bonds and then reforming them in a new configuration, maintaining the hair in the new shape for 3 – 6 months, providing a bright, silky effect with natural look.
This document provides instructions for performing an enzyme-linked immunosorbent assay (ELISA) to quantitatively detect total aflatoxins (B1, B2, G1, and G2) in grains, nuts, and other commodities. Aflatoxins are toxic metabolites produced by certain molds that can contaminate foods and animal feeds. The ELISA uses an aflatoxin-specific antibody to competitively bind sample aflatoxins or HRP-conjugated aflatoxin. The intensity of the color reaction indicates the aflatoxin concentration in samples, which can be quantified by comparison to standard curves. Validation studies showed the assay can reliably detect aflatoxins from 1-20 p
Principles & Applications of cell viability assays (MTT Assays)VidyaNani
This document discusses cell viability assays, specifically focusing on principles and applications of MTT assays. It defines cell viability and describes various types of cell viability assays including dye exclusion, colorimetric, fluorometric, luminometric, and flow cytometric assays. The document provides details on the MTT assay, including its principle, protocol, and applications for measuring cell proliferation, cytotoxicity, and metabolic activity. The MTT assay is a common colorimetric assay that measures the reduction of yellow MTT by mitochondrial dehydrogenases in viable cells to produce purple formazan crystals.
At Apollo Hospital, Lucknow, U.P., we provide specialized care for children experiencing dehydration and other symptoms. We also offer NICU & PICU Ambulance Facility Services. Consult our expert today for the best pediatric emergency care.
For More Details:
Map: https://cutt.ly/BwCeflYo
Name: Apollo Hospital
Address: Singar Nagar, LDA Colony, Lucknow, Uttar Pradesh 226012
Phone: 08429021957
Opening Hours: 24X7
COPD Treatment in Ghatkopar,Mumbai. Dr Kumar DoshiDr Kumar Doshi
Are you or a loved one affected by Chronic Obstructive Pulmonary Disease (COPD)? Discover comprehensive and advanced treatment options with Dr. Kumar Doshi, a preeminent COPD specialist based in Ghatkopar, Mumbai.
Dr. Kumar Doshi is dedicated to delivering the highest standard of care for COPD patients. Whether you are seeking a diagnosis, a second opinion, or exploring new treatment avenues, this presentation will guide you through the exceptional services available at his practice in Ghatkopar, Mumbai.
More Related Content
Similar to MINOXODIL RESPONSE TEST to assess efficacy.pptx
This document provides an overview of preformulation studies for intramuscular injections. It discusses various parameters studied in preformulation including organoleptic properties, particle size, shape and distribution, powder flow properties, FTIR spectroscopy, DSC, X-ray diffraction, solubility, partition coefficient, and the effect of temperature on solubility. It also describes stability studies conducted at different stages including stress testing, accelerated and long-term testing to determine shelf life and storage conditions. The testing scope for liquid injectables includes parameters like pH, clarity, assay, degradation products, and functionality of container closure systems. Finally, it discusses climatic zones for stability testing and the hygroscopic nature of some drug substances.
Different Laboratory Equipment used in Toxicology and Molecular BiologyMuhammad Kamran (Sial)
This document describes various types of laboratory equipment used in toxicology and molecular biology. It discusses personal protective equipment, volume measuring tools like beakers and volumetric flasks, microscopes for identification, analyzers like microplate readers and electrophoresis apparatus, chromatography equipment, and other tools like refrigerators, sterilizers, and centrifuges. The functions, components, principles, and applications of these different pieces of equipment are explained.
The beautiful hair, how to grind hair sample for detectionsWISBIOMED
The document discusses guidelines for using human hair samples for drug testing. It provides details on:
- Properly collecting and storing hair samples, including recommended sampling locations, recording hair characteristics, defining root and tip sections, and minimizing degradation or contamination.
- Cleaning hair samples through solvent washes to remove external contaminants before analysis.
- Methods for disintegrating hair including grinding it into a powder using Precellys, an instrument that pulverizes samples through bead beating.
- Carrying out extraction and various drug tests on processed hair samples, including for opiates, cocaine, amphetamines, and cannabinoids.
High performance thin layer chromatography RevanHiwale1
This document describes a high performance thin layer chromatography (HPTLC) method for quantifying paracetamol, pseudoephedrine, and loratadine in pharmaceutical tablets and human plasma. The method involves separating the drugs on HPTLC plates using an acetone-hexane-ammonia mobile phase. Calibration curves are generated by plotting peak areas versus known concentrations of each drug. The method is then applied to analyze pharmaceutical tablets and human plasma samples spiked with the drugs. Results show the HPTLC method can successfully separate and quantify the three drugs simultaneously despite their complex concentration ratios in pharmaceutical formulations.
Those who administer ionizing radiation must become familiar with the magnitude of exposure encountered in medicine, dentistry and every day life; the possible risks associated with such exposure; and the methods used to affect exposure.
Practitioners should remain informed about safety updates to further improve diagnostic quality of radiographs and decrease radiation exposure.
External contamination of hair with heroin was evaluated in six volunteers over 3 months. All contaminated subjects tested positive for opiates like heroin, 6-MAM, morphine and acetylcodeine for at least 3 months after the contamination period. Significant levels of 6-MAM (>0.5 ng/mg) were detected until 6 weeks in all subjects. The 6-MAM/morphine ratio was always above 1.3, even 3 months after contamination. Decontamination procedures were not sufficient to remove drugs that had penetrated into hair from external contamination. This suggests a risk of false positives from external contamination when interpreting hair drug tests.
This document describes the methods for conducting an in vitro chromosomal aberration test. White blood cells are cultured from human donors and exposed to test substances like cyclophosphamide. Cells are harvested at different timepoints throughout the cell cycle. Slides are prepared, stained, and analyzed under a microscope to identify chromosomal abnormalities like deletions, duplications, inversions, and translocations. Acceptance criteria include having homogenous results between replicates, aberration frequencies within historical controls, and a concentration-dependent response to a test substance. A conclusion on the potential of a substance to induce chromosomal aberrations is based on statistical analyses.
Determination of Etodolac in Commercial Formulations by HPLC-UV Methodijtsrd
The aim of this study was to develop and verify a simple, rapid and sensitive high performance liquid chromatography method coupled with UV detector HPLC UV method for the quantitative determination of etodolac in bulk and pharmaceutical dosage forms. Chromatographic separation was performed at ambient conditions on a reverse phase ACE C8 analytical column 250 mm x 4.6 mm ID, 5 umm using the mobile phase containing acetonitrile water 80 20, v v at a flow rate of 1.0 mL min 1. A wavelength of 272 nm was used for etodolok and paracetamol IS . A retention time of 4.21 min and 2.02 min were obtained for etodolac and IS, respectively. The method showed linearity in the range of 0.08 10 µg mL 1 for etodolac R = 0.9999 . The linear regression equations obtained by least square regression method were the ratio of peak area of etodolac and IS =1.559 concentration etodolac µg mL 0.139. The intra day and inter day RE and RSD values of the method were =10.0 and =2.65 , respectively. Limit of detection LOD and limit of quantification LOQ were found to be 0.04 and 0.06 µg mL 1 for etodolac, respectively. A new, simple and sensitive high performance liquid chromatography method was developed and validated for etodolac. The method can be applied for the quantification of etodolac without derivatization in bulk solutions and commercial formulations using the internal standard. Tugrul Cagri Akman | Yucel Kadioglu "Determination of Etodolac in Commercial Formulations by HPLC-UV Method" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29452.pdfPaper URL: https://www.ijtsrd.com/pharmacy/analytical-chemistry/29452/determination-of-etodolac-in-commercial-formulations-by-hplc-uv-method/tugrul-cagri-akman
UV Spectrophotometeric Analytical Method Development and Validation for the D...ijtsrd
An easy, simple, specific, speedy, precise and accurate have been developed and validated for content determination of Telmisartan. This article based on validation of analytical method procedures which is established in ICH Q2 R1 .Telmisartan demonstrated the absorption maxima in at 291.2 nm and found was linear for a range of 5 µg ml ”“25 µg ml with correlation coefficient LOD of Telmisartan was found to be 2.09µg ml and the limit of quantification LOQ of Telmisartan was found to be 6.34 µg ml. The analytical method validation of the above proposed method was performed by carrying out precision and accuracy studies. The Accuracy percentage recovery on three different levels i.e. 25 , 50 and 75 was found to be 95.20 , 94.21 and 90.95 respectively. The proposed analytical method demonstrated good Intra precision Repeatability with relative standard deviations 2.54. The proposed analytical method was validated for the test parameter Specificity, Precision, Linearity and range, Ruggedness, Accuracy and recovery. The proposed method for content determination of Telmisartan in pureand tablet dosage form by UV spectrophotometer in pharmaceutical found easy, simple, accurate, precise and reproducible, economical and can be applied for the everyday quality control analysis. C. Shambiga | M. Menaka "UV Spectrophotometeric Analytical Method Development and Validation for the Determination of Telmisartan in Pharmaceutical Drug and Drug Formulation (Tablet Dosage Form)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-7 | Issue-3 , June 2023, URL: https://www.ijtsrd.com.com/papers/ijtsrd56293.pdf Paper URL: https://www.ijtsrd.com.com/pharmacy/other/56293/uv-spectrophotometeric-analytical-method-development-and-validation-for-the-determination-of-telmisartan-in-pharmaceutical-drug-and-drug-formulation-tablet-dosage-form/c-shambiga
This document provides instructions for using an ELISA kit to detect the mycotoxin zearalenone in cereal crops and animal feeds. It begins with an introduction to zearalenone and its health effects. It then describes the intended use, principle, reagents, materials, precautions, extraction procedure, and assay procedure for the zearalenone ELISA kit. The kit is designed to quantitatively detect zearalenone in samples through a competitive enzyme immunoassay.
Determinación por FISH de la deleción del cromosoma 7.pdfEduardoMasat1
This document provides troubleshooting recommendations for potential problems that may occur during fluorescence in situ hybridization (FISH). It lists several potential causes for issues like no FISH signals, weakening hybridization signals over time, diffuse signals, weak signals, and high background signals. Recommended solutions are provided for each potential cause, such as checking the microscope optics, adjusting temperatures and concentrations, and ensuring proper sample preparation. Customer support contact information is also included.
PureLizz CST-50 A Revolutionary System for Hair Reshaping developed by Pure K...Daniel Marks
PureLizz CST-50 is the safest and most effective way to change the structural hair fiber into either curly or straight, or as desired by the professional hairdresser.
PureLizz System is a unique and safe process, promoting the cleavage of the keratin disulfide bonds and then reforming them in a new configuration, maintaining the hair in the new shape for 3 – 6 months, providing a bright, silky effect with natural look.
This document provides instructions for performing an enzyme-linked immunosorbent assay (ELISA) to quantitatively detect total aflatoxins (B1, B2, G1, and G2) in grains, nuts, and other commodities. Aflatoxins are toxic metabolites produced by certain molds that can contaminate foods and animal feeds. The ELISA uses an aflatoxin-specific antibody to competitively bind sample aflatoxins or HRP-conjugated aflatoxin. The intensity of the color reaction indicates the aflatoxin concentration in samples, which can be quantified by comparison to standard curves. Validation studies showed the assay can reliably detect aflatoxins from 1-20 p
Principles & Applications of cell viability assays (MTT Assays)VidyaNani
This document discusses cell viability assays, specifically focusing on principles and applications of MTT assays. It defines cell viability and describes various types of cell viability assays including dye exclusion, colorimetric, fluorometric, luminometric, and flow cytometric assays. The document provides details on the MTT assay, including its principle, protocol, and applications for measuring cell proliferation, cytotoxicity, and metabolic activity. The MTT assay is a common colorimetric assay that measures the reduction of yellow MTT by mitochondrial dehydrogenases in viable cells to produce purple formazan crystals.
Similar to MINOXODIL RESPONSE TEST to assess efficacy.pptx (20)
At Apollo Hospital, Lucknow, U.P., we provide specialized care for children experiencing dehydration and other symptoms. We also offer NICU & PICU Ambulance Facility Services. Consult our expert today for the best pediatric emergency care.
For More Details:
Map: https://cutt.ly/BwCeflYo
Name: Apollo Hospital
Address: Singar Nagar, LDA Colony, Lucknow, Uttar Pradesh 226012
Phone: 08429021957
Opening Hours: 24X7
COPD Treatment in Ghatkopar,Mumbai. Dr Kumar DoshiDr Kumar Doshi
Are you or a loved one affected by Chronic Obstructive Pulmonary Disease (COPD)? Discover comprehensive and advanced treatment options with Dr. Kumar Doshi, a preeminent COPD specialist based in Ghatkopar, Mumbai.
Dr. Kumar Doshi is dedicated to delivering the highest standard of care for COPD patients. Whether you are seeking a diagnosis, a second opinion, or exploring new treatment avenues, this presentation will guide you through the exceptional services available at his practice in Ghatkopar, Mumbai.
We are one of the top Massage Spa Ajman Our highly skilled, experienced, and certified massage therapists from different corners of the world are committed to serving you with a soothing and relaxing experience. Luxuriate yourself at our spas in Sharjah and Ajman, which are indeed enriched with an ambiance of relaxation and tranquility. We could confidently claim that we are one of the most affordable Spa Ajman and Sharjah as well, where you can book the massage session of your choice for just 99 AED at any time as we are open 24 hours a day, 7 days a week.
Visit : https://massagespaajman.com/
Call : 052 987 1315
Unlocking the Secrets to Safe Patient Handling.pdfLift Ability
Furthermore, the time constraints and workload in healthcare settings can make it challenging for caregivers to prioritise safe patient handling Australia practices, leading to shortcuts and increased risks.
Comprehensive Rainy Season Advisory: Safety and Preparedness Tips.pdfDr Rachana Gujar
The "Comprehensive Rainy Season Advisory: Safety and Preparedness Tips" offers essential guidance for navigating rainy weather conditions. It covers strategies for staying safe during storms, flood prevention measures, and advice on preparing for inclement weather. This advisory aims to ensure individuals are equipped with the knowledge and resources to handle the challenges of the rainy season effectively, emphasizing safety, preparedness, and resilience.
MYASTHENIA GRAVIS POWER POINT PRESENTATIONblessyjannu21
Myasthenia gravis is a neurological disease. It affects the grave muscles in our body. Myasthenia gravis affects how the nerves communicate with the muscles. Drooping eyelids and/or double vision are often the first noticeable sign. It is involving the muscles controlling the eyes movement, facial expression, chewing and swallowing. It also effects the muscles neck and lip movement and respiration.
It is a neuromuscular disease characterized by abnormal weakness of voluntary muscles that improved with rest and the administration of anti-cholinesterase drugs.
The person may find difficult to stand, lift objects and speak or swallow. Medications and surgery can help the patient to relieve the symptoms of this lifelong illness.
Let's Talk About It: Breast Cancer (What is Mindset and Does it Really Matter?)bkling
Your mindset is the way you make sense of the world around you. This lens influences the way you think, the way you feel, and how you might behave in certain situations. Let's talk about mindset myths that can get us into trouble and ways to cultivate a mindset to support your cancer survivorship in authentic ways. Let’s Talk About It!
Chandrima Spa Ajman is one of the leading Massage Center in Ajman, which is open 24 hours exclusively for men. Being one of the most affordable Spa in Ajman, we offer Body to Body massage, Kerala Massage, Malayali Massage, Indian Massage, Pakistani Massage Russian massage, Thai massage, Swedish massage, Hot Stone Massage, Deep Tissue Massage, and many more. Indulge in the ultimate massage experience and book your appointment today. We are confident that you will leave our Massage spa feeling refreshed, rejuvenated, and ready to take on the world.
Visit : https://massagespaajman.com/
Call : 052 987 1315
As Mumbai's premier kidney transplant and donation center, L H Hiranandani Hospital Powai is not just a medical facility; it's a beacon of hope where cutting-edge science meets compassionate care, transforming lives and redefining the standards of kidney health in India.
The facial nerve, also known as cranial nerve VII, is one of the 12 cranial nerves originating from the brain. It's a mixed nerve, meaning it contains both sensory and motor fibres, and it plays a crucial role in controlling various facial muscles, as well as conveying sensory information from the taste buds on the anterior two-thirds of the tongue.
MBC Support Group for Black Women – Insights in Genetic Testing.pdfbkling
Christina Spears, breast cancer genetic counselor at the Ohio State University Comprehensive Cancer Center, joined us for the MBC Support Group for Black Women to discuss the importance of genetic testing in communities of color and answer pressing questions.
Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...Ear Solutions (ESPL)
Binaural hearing using two hearing aids instead of one offers numerous advantages, including improved sound localization, enhanced sound quality, better speech understanding in noise, reduced listening effort, and greater overall satisfaction. By leveraging the brain’s natural ability to process sound from both ears, binaural hearing aids provide a more balanced, clear, and comfortable hearing experience. If you or a loved one is considering hearing aids, consult with a hearing care professional at Ear Solutions hearing aid clinic in Mumbai to explore the benefits of binaural hearing and determine the best solution for your hearing needs. Embracing binaural hearing can lead to a richer, more engaging auditory experience and significantly improve your quality of life.
2. MRT
Instructions for Use
The MINOXIDIL RESPONSE TEST is a prescription In-vitro diagnostic
(IVD) device to be used by a physician as an aid in assessing the
likelihood that a patient suffering from pattern hair loss will not
respond to 5% topical minoxidil.
Intended Use
MINOXIDIL RESPONSE TEST quantitatively measures sulfotransferase
(SULT) activity in the bulb of a plucked human hair sample. When
performed according to these instructions, the results of this assay
may be used to rule out non-responders to the topical 5% minoxidil
for the treatment of androgenetic alopecia. This device is for in vitro
diagnostic use by a physician only. Federal law restricts this device to
sale by or on the order of a physician.
3. MRT
Summary and Explanation of the Test
Sulfotranseferase (SULT) is an enzyme that catalyzes the transfer of a
sulfo group from a donor molecule to an acceptor alcohol. The enzyme
has wide human tissue distribution, including the liver and outer root
sheath (ORS) of the hair follicle. Inter-individual variation in sulfonation
capacity is important in determining an individual's metabolism to
certain xenobiotics.
Minoxidil is one drug that requires sulfonation to have efficacy. The
sulfonation capacity of an individual’s ORS can be used to rule out non-
responders to 5% minoxidil for the treatment of androgenetic alopecia.
Principles of the Procedure
MINOXIDIL RESPONSE TEST measures inter-individual variation in
sulfonation capacity.
Endogenous SULT enzymes in the ORS of plucked hair are reacted with
the test solution by immersing the hair bulb and incubating. After a
period of time, the color intensity of the solution is measured
photometrically. The color intensity of the solution depends on the level
of active SULT present in the ORS and can be used to rule out non-
responders to the drug minoxidil.
6. Materials
Requiredbut
notprovided
A calibrated spectrophotometer device capable of reading
absorption at a wavelength of 405nm with a resolution of at least
± 0.01 OD and a minimum range of 0.0 1.0 OD (e.g., Shimadzu UV
1700).
(Note: reacted MRT can be diluted with an optically transparent
solvent (e.g., water) to facilitate filling of cuvettes, as long as the
dilution does not reduce the signal (sample OD) below the limit of
detection of the instrument. Additionally, if a solvent is used to dilute
samples an appropriate scaling factor (dilution factor) must be
applied to the raw OD reading.)
Pipettes capable of accurately delivering 10 to 200μl.
Disposable pipette tips.
Clean 0.5 ml polypropylene sample tubes (e.g. Seal-Rite® 0.5 mL
microfuge tube, USA Scientific)
Laboratory timer to monitor incubation steps
7. Precautions
For in vitro diagnostic use only.
Normal precautions exercised in handling laboratory reagents should be
followed. In case of contact with eyes, rinse immediately with plenty of
water and seek medical advice. Wear suitable protective clothing, gloves,
and eye/face protection. Do not breathe vapor. Dispose of waste observing
all local, state, and federal laws.
Adherence to the specified time and temperature of incubations is essential
for accurate results.
MRT should be keep refrigerated when not in use.
Dilution or adulteration of these reagents may generate erroneous results.
Reagents from other sources or manufacturers should not be used.
Avoid contact of reagents and patient specimens with skin and mucous
membranes.
Avoid microbial contamination in reagents. Incorrect results may occur.
Cross contamination of reagents and/or samples could cause erroneous
results.
Do not use MRT beyond expiration date. Incorrect results may occur.
8. QualityControl
&
StorageConditions
Quality Control:
The company has established a complete Quality System in
accordance with 21 CFR Quality System Regulations. This
includes maintenance of all records associated with the
manufactures Device Master Record and Design History File.
Storage Conditions:
• MRT Solution: Store between 2° and 8°C.
• Properly stored, MRT Solution is stable for 90 day
• Discard solution if it appears yellow.
• DO NOT FREEZE. Discard if frozen.
9. Specimen
Collection
&
Preparation for
Analysis
Pluck 10 hairs from the area just outside (~1 inch) of the balding
region.
It is best to use tweezers and pluck one hair at a time.
Visually inspect the hairs to make sure they have the bulb (the
bulbs are larger in diameter than the hair shaft).
Plucked hair samples containing bulb (ORS) can be stored at room
temperature in clean sample tube or bag for up to 6 days.
Samples should be processed or stored within 6 days of plucking.
Samples may be stored at -80° C for up to 5 years if stored within 6
days plucking.
Samples may be stored at -20° C for up to 1 month if stored within
6 days plucking.
Hairs should be visually inspected for the presence of hair bulb
prior to performing the test as hairs without bulbs may produce
an erroneous result. If bulbs are too difficult to see, a dissecting
microscope (40X) may be used.
10. General
Procedure
Trim plucked hairs containing bulb to a length of ~1cm with
scissors.
Place 2 hairs containing bulbs at the bottom of a clean 0.5 mL
sample tube.
Pipette 100 μl of MRT Solution into the tube so that the hair bulbs
are immersed.
Repeat steps 1-3 to produce a total of 4 sample replicates per
patient (8 hairs total, 2 hairs per sample)
Pipette 100 μl of MRT Solution into a clean tube. This will serve
as a negative control (blank).
Close tubes and spin down insure hair bulbs are immersed
Incubate samples and control (blank) for 16-24 hours at room
temperature (20-25°C).
Remove and discard hairs.
Read samples at a wavelength of 405 nm and measure the
optical density (OD) of each sample. Determine the average
of the 4 replicate samples. Read the negative control (blank)
sample at a wavelength of 405 nm and measure the optical
density (OD). Subtract the OD of the negative control from
the average of the 4 replicate samples.
15. Performance
Characteristics
The final result of the Minoxidil Response Test is interpreted as
follows:
• Clinical studies of the Minoxidil Response Test showed test
values can range from 3%
• - 300% of the 0.4 OD cut-off (min=0.012 and max=1.348,
respectively).
• In a clinical study, the biological (within-subject) variability of
the sulfotransferase enzyme in plucked hair samples was
determined to be 0.2 OD using 1 replicate of 2 hairs. Four (4)
replicates of 2 hairs are required to achieve the positive
predictive value (PPV) and negative predictive value (NPV)
reported in this document.
16. Linearity
&
Precisionofthe
method
Linearity:
The Minoxidil Response Test is linear around the cut-off
value of 0.4 OD. It is not suitable for quantitative
measurement of sulfotransferase.
Precision of the method:
Analytical method validation was performed following CLSI
EPO5-A3 guidelines and precision was assessed at 25%, 50%,
75%, 100%, 125%, 150%, 175%, & 200% of the
0.4 OD cutoff value. One hundred percent (100%) precision was
shown at ≤ 75% and
≥ 125% of the 0.4 OD cutoff (≤ 0.3 OD and ≥ 0.5 OD respectively).
17. Directions
GENTLY TAP LID SO LIQUID COALESCES.
PLUCK TWO HAIRS FROM SCALP WITH TWEEZERS. CHECK TO MAKE SURE EACH
HAIR SAMPLE HAS A VISIBLE HAIR BULB.
REMOVE CAP AND INSERT HAIRS. ENSURE HAIR BULBS ARE COMPLETELY
SUBMERSED INTO LIQUID.
TRIM ANY HAIR PROTRUDING FROM THE TOP OF THE TUBE WITH A CLEAN PAIR
OF SCISSORS.
REPLACE CAP, KEEPING VIAL IN AN UPRIGHT POSITION.
STORE AT ROOM TEMPERATURE FOR 60 MINUTES.