This document presents the results of a study analyzing the elemental composition of various milk samples using inductively coupled plasma mass spectrometry (ICP-MS). Milk samples from different brands and targeted at different age groups were solubilized using N,N-dimethylformamide (DMF) and analyzed for various elements. Optimal instrument parameters like argon flow rate, radio frequency power, and sample flow rate were determined. Concentrations of elements like As, Co, Cu, Mn, Mo, Ni, Rb, S, Sr, V, Zn were measured and compared across milk samples. Reference materials were also analyzed to validate the method, which demonstrated accurate recovery of elements within certified ranges.
The circulatory system carries blood and substances throughout the body using the heart and blood vessels. The heart pumps blood into two circuits - the pulmonary circuit which pumps to the lungs, and the systemic circuit which pumps to the rest of the body. Blood travels from the heart through arteries, then into capillaries to exchange materials, and returns to the heart through veins. The circulatory system allows for the transport of oxygen, nutrients, wastes and more throughout the body.
The document discusses the roles of chemical elements in plants and analytical techniques for analyzing plant samples. It describes essential major and micronutrients for plants as well as toxic elements. It then provides details on elemental analysis techniques including EA for analyzing carbon, hydrogen, nitrogen, sulfur, and oxygen, as well as ICP-AES for determining various elements. ICP-AES involves atomizing a sample in a plasma and detecting element-specific emission spectra. Sample preparation such as microwave digestion is often required prior to analysis.
This presentation summarizes the role of pretreatment processes in enhancing anaerobic digestion of municipal solid waste. Various pretreatment methods including mechanical, thermal, chemical and combinations are discussed. A case study on microbial pretreatment of lignocellulosic waste showed improved biogas and methane production. Pretreatments can increase biodegradability, biogas yields, degradation rates and reduce retention times compared to untreated processes. Thermal pretreatments at low temperatures are often most cost-effective.
This document provides an overview of plant mineral analysis techniques. It discusses the essential and toxic elements analyzed in plants, sample pretreatment including sampling, decontamination, drying and grinding. Sample preparation techniques like dry ashing and wet ashing are described. The instrumentation used for analysis including XRF, AAS, flame emission spectrometry, ICP-AES, UV-VIS spectrophotometry and elemental analyzers are outlined. Key concepts like qualitative and quantitative analysis, calibration curves, limits of detection and quantitation, accuracy and precision are also summarized.
Sample preparation techniques of solid dosage formsSathish Vemula
Knife mills and blenders are particle size reduction techniques that can be used to disperse solid dosage forms like non-disintegrating tablets for analysis. Mechanical techniques like grinding with a mortar and pestle or milling in a ball mill are commonly used to reduce the particle size and facilitate extraction of drugs from solid oral dosage forms. Agitation techniques like shaking, stirring, vortexing, and sonication are then used to further facilitate dispersion and mixing during sample preparation. The selection of appropriate particle size reduction and agitation techniques depends on the properties of the specific drug product and dosage form being analyzed.
Extraction and phytochemical analysis of medicinal plantsShameem_Byadgi
This study investigated the phytochemical constituents and total phenolic content of several medicinal plants. Plant materials were extracted using different solvents and subjected to qualitative and quantitative analysis. Qualitative testing identified the presence of compounds like phenols, tannins, flavonoids, saponins, alkaloids, etc. Total phenolic and flavonoid content was determined using spectrophotometric methods. The results showed that the plants contained valuable phytochemicals and could be a potential source of drugs. The methanol extracts generally had the highest concentration of phytochemicals.
1) The document reports analysis results from various plants and parameters including sweet gas, feed gas, lean gas, C2+, ethylene product, propylene product, quench oil, extruder pellets, drum waters, cooling waters, and ETP discharge.
2) Measurement parameters include composition percentages, flow rates, pH, conductivity, hardness, alkalinity, chlorides, silica, phosphates, and more.
3) Results are within specified ranges for all samples and parameters measured on July 31st, 2022.
The circulatory system carries blood and substances throughout the body using the heart and blood vessels. The heart pumps blood into two circuits - the pulmonary circuit which pumps to the lungs, and the systemic circuit which pumps to the rest of the body. Blood travels from the heart through arteries, then into capillaries to exchange materials, and returns to the heart through veins. The circulatory system allows for the transport of oxygen, nutrients, wastes and more throughout the body.
The document discusses the roles of chemical elements in plants and analytical techniques for analyzing plant samples. It describes essential major and micronutrients for plants as well as toxic elements. It then provides details on elemental analysis techniques including EA for analyzing carbon, hydrogen, nitrogen, sulfur, and oxygen, as well as ICP-AES for determining various elements. ICP-AES involves atomizing a sample in a plasma and detecting element-specific emission spectra. Sample preparation such as microwave digestion is often required prior to analysis.
This presentation summarizes the role of pretreatment processes in enhancing anaerobic digestion of municipal solid waste. Various pretreatment methods including mechanical, thermal, chemical and combinations are discussed. A case study on microbial pretreatment of lignocellulosic waste showed improved biogas and methane production. Pretreatments can increase biodegradability, biogas yields, degradation rates and reduce retention times compared to untreated processes. Thermal pretreatments at low temperatures are often most cost-effective.
This document provides an overview of plant mineral analysis techniques. It discusses the essential and toxic elements analyzed in plants, sample pretreatment including sampling, decontamination, drying and grinding. Sample preparation techniques like dry ashing and wet ashing are described. The instrumentation used for analysis including XRF, AAS, flame emission spectrometry, ICP-AES, UV-VIS spectrophotometry and elemental analyzers are outlined. Key concepts like qualitative and quantitative analysis, calibration curves, limits of detection and quantitation, accuracy and precision are also summarized.
Sample preparation techniques of solid dosage formsSathish Vemula
Knife mills and blenders are particle size reduction techniques that can be used to disperse solid dosage forms like non-disintegrating tablets for analysis. Mechanical techniques like grinding with a mortar and pestle or milling in a ball mill are commonly used to reduce the particle size and facilitate extraction of drugs from solid oral dosage forms. Agitation techniques like shaking, stirring, vortexing, and sonication are then used to further facilitate dispersion and mixing during sample preparation. The selection of appropriate particle size reduction and agitation techniques depends on the properties of the specific drug product and dosage form being analyzed.
Extraction and phytochemical analysis of medicinal plantsShameem_Byadgi
This study investigated the phytochemical constituents and total phenolic content of several medicinal plants. Plant materials were extracted using different solvents and subjected to qualitative and quantitative analysis. Qualitative testing identified the presence of compounds like phenols, tannins, flavonoids, saponins, alkaloids, etc. Total phenolic and flavonoid content was determined using spectrophotometric methods. The results showed that the plants contained valuable phytochemicals and could be a potential source of drugs. The methanol extracts generally had the highest concentration of phytochemicals.
1) The document reports analysis results from various plants and parameters including sweet gas, feed gas, lean gas, C2+, ethylene product, propylene product, quench oil, extruder pellets, drum waters, cooling waters, and ETP discharge.
2) Measurement parameters include composition percentages, flow rates, pH, conductivity, hardness, alkalinity, chlorides, silica, phosphates, and more.
3) Results are within specified ranges for all samples and parameters measured on July 31st, 2022.
Changes in levels of bio-chemicals and secondary metabolites during peak stre...ILRI
Presented by Ephraim Nuwamanya, Patrick R Rubaihayo, Settumba Mukasa, Samuel Kyamanywa, Robert Kawuki, Joseph Hawumba and Yona Baguma (NaCRRI/MAK) at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013
Presentation by Dr. Sarah Cianférani-Sanglier, University of Strasbourg, Strasbourg, France. Talk given at Waters Antibody Drug Conjugates (ADC) 2014 Meeting, Nov. 20-21, Wilmslow UK.
This document summarizes cluster analysis performed on sales data containing continuous variables for products like fresh, milk, and frozen goods, and nominal variables for store channel and region. Univariate analysis and scaling were performed, and two clusters were identified as the optimal number. Cluster 1 contains 135 data points and cluster 2 contains 305 points. The cluster means show differences between the two clusters for each variable.
EFFECT OF DIETARY ORGANIC SELENIUM SUPPLEMENTATION ON MASS AND ENZYME ACTIVIT...kachiwal Allah Bux
The document discusses a study that evaluated the effect of dietary organic selenium supplementation on rumen microbes and enzyme activities in goats. The study found that high concentrate diets and selenium supplementation increased short chain fatty acid concentrations and microbial populations in the rumen compared to goats on a basal diet only. While there were no significant differences between just the high concentrate diet and the high concentrate diet with selenium, rumen fermentation patterns were slightly better with selenium addition. The study concludes that selenium supplementation can improve rumen fermentation when goats are fed high concentrate diets.
The document reports on a whole genome scan that identified quantitative trait loci (QTL) associated with milk fat composition in summer milk. Four significant QTL were detected on chromosomes 14, 15, 26 and 27 that influence levels of saturated and unsaturated fatty acids. The QTL on chromosome 14 had the largest effect, influencing most fatty acid traits. Comparison to a previous study of winter milk composition found some consistent QTL on chromosomes 14 and 26, but many differences in QTL between the two seasons, likely due to environmental and design effects. The results provide opportunities for marker-assisted selection to genetically modify milk fat composition.
Stabilization of Pickering emulsion using Octenyl succinic anhydride (OSA) mo...DCSaxena1
This document summarizes research stabilizing Pickering emulsions using octenyl succinic anhydride (OSA) modified buckwheat starch. Key findings include:
- OSA modification increased buckwheat starch particle size and irregularity while decreasing amylose content. Higher OSA concentration led to greater changes.
- OSA modified buckwheat starch stabilized Pickering emulsions for 30 days with no creaming or sedimentation under high-intensity ultrasound, unlike native starch.
- Microscopic images showed OSA starch coated soybean oil droplets and prevented coalescence for 30 days. Droplet size increased slightly over time but remained nanoscale.
- OSA modification improved buckwheat starch's ability to
EPA Method 200.7, Trace Elements in Water, Solids, and Biosolids by Inductively Coupled Plasma-Atomic Emission Spectrometry, describes the procedure and requirements for multi-element determinations by ICP-AES. This presentation demonstrates the capability of the ICPE-9820, with the ASC-9800 Auto-sampler and the Standard Addition Kit, to produce quick, accurate results that comply with the method.
This monthly report summarizes Nuttapon Khongdee's work on analyzing heavy metal content, pH, EC, and organic carbon levels in different soil types before planting. Soil samples were tested for pH, EC, and organic carbon using calibrated instruments and standard procedures. Results are displayed in tables showing mean and standard deviation values for each soil type and treatment. The report also discusses initial work on characterizing citral oil nanoemulsions, including how formulation components like HLB value, homogenization speed, and oil/surfactant ratio influence particle size and stability over time. Future work is planned to analyze the nanoemulsions' antibacterial and antifungal activities.
STUDIES ON INTEGRATED BIO-HYDROGEN PRODUCTION PROCESS-EXPERIMENTAL AND MODELINGArghya_D
In the project “Studies on integrated biohydrogen production process-Experimental and Modeling”,a co-culture (mixture of two microorganisms in a single reactor) study of a dark fermentative and photofermentative microorganism was done to assess its hydrogen production performance. For modeling purpose, Artificial Neural Network and Genetic Algorithm has been used as a stochastic technique. The optimized data from batch study was successfully used to run a photobioreactor in continuous mode. A mechanistic model was developed for a continuous co-culture setup using data from literature and solved using MATLAB.
Atlantic mackerel protein hydrolysate was fractionated using electro-driven ultrafiltration (EDUF), pressure-driven ultrafiltration, and solid phase extraction to isolate immunomodulatory peptides. Fractions were tested for effects on nitric oxide production in macrophages as a measure of anti-inflammatory activity. pH 3 fractions increased nitric oxide, while the hydrophobic fraction from solid phase extraction decreased nitric oxide production, indicating anti-inflammatory activity. Amino acid analysis found the charged fractions had higher essential amino acid content than other fractions. EDUF fractions contained peptides from 2-6 amino acids in size and were identified as coming from protein precursors through mass spectrometry.
The document discusses turbidimetry and nephelometry techniques for measuring turbidity. Turbidity is caused by particles in a liquid that scatter light making the liquid appear cloudy. The intensity of turbidity depends on factors like particle quantity, size, shape, and wavelength of scattered radiation. Turbidimetry measures how light is absorbed as it passes through a sample, while nephelometry measures light scattered at an angle. Both techniques are used to analyze water quality, ions that form insoluble compounds, and microbial growth. Immunoturbidimetry uses the same principles to quantify antigens and antibodies in serum.
This document discusses calibration standards for mass spectrometers used in stable isotope analysis. It presents isotopic reference values for several calibration standards including AtlantisII, Atlantis3, ULAWATU, ESTREMOZ, BORBA, PDBBB, GoMLoP, and UCD-SM92. It also shows results from interlaboratory comparisons and discusses the need for 3-point slope corrections to improve calibration accuracy between laboratories. Suggestions are provided to standardize calibration procedures and better account for instrumental differences.
1) The document contains GDP and inflation data for various sectors in Indonesia from 2008-2018.
2) Statistical tests were performed to test the classical assumptions of the data, including normality, linearity, autocorrelation, and heteroskedasticity. The results of the tests show that the data satisfies the assumptions.
3) Multicollinearity was also tested using correlation analysis and regressing the independent variables against each other. The results show evidence of multicollinearity between some variables.
Challenges of Analyzing Petrochemicals and Organic SolventsRyan Brennan
This document discusses optimizing an ICP system for analyzing organic solvents and oils. It recommends using a low uptake nebulizer, inline particle filter, and spray chamber designed for solvents. A temperature controlled nebulizer can maintain samples from -10 to -25°C, improving volatility. A demountable torch reduces costs and a dilution probe automates oil dilution and injection into the ICP for precise analysis of wear metals.
This document characterizes polyphenol oxidase (PPO) in Ataulfo mango. It finds that PPO activity varies depending on the substrate and pH level, with optimal activity between pH 5.4-6.4. Sodium ascorbate, glutathione, and kojic acid show promise as PPO inhibitors. PPO activity is higher in mango skin than pulp. Gel electrophoresis identified a prominent PPO protein band at 53 kDaltons. The study provides information on PPO kinetics, inhibition effects, and variation between mango tissues to characterize PPO in Ataulfo mango.
Practical Implementation of the New Elemental Impurities Guidelines May 2015SGS
The International Conference on Harmonization (ICH) released its Q3D Guideline for Elemental Impurities in December 2014, initiating reviews and changes in quality testing programs in bio/pharmaceutical companies around the world. In advance of the implementation dates, companies need to assess the risks of potential elemental impurities in their process and materials streams.
In this presentation, experts will review the requirements of elemental impurities guidelines from ICH, the European Pharmacopeia, and United States Pharmacopeia, outline practical recommendations to address implementation challenges, and discuss key considerations for analytical testing programs.
Development and validation of rp hplc method for simultaneouschandu chatla
The document describes the development and validation of an RP-HPLC method for the simultaneous determination of tramadol hydrochloride, paracetamol, and dicyclomine hydrochloride in pharmaceutical formulations. The method utilizes a central composite design of experiments to optimize the chromatographic separation. Key factors investigated include mobile phase composition, pH, and flow rate. The optimized method is validated per ICH guidelines and demonstrates suitable linearity, precision, accuracy, and robustness for quality control applications.
Purification optimization and characterization of protease from Bacillus va...Vaibhav Maurya
This presentation is a research work carried out by me in B.Tech 8 semester. and gives an idea about purification, optimization and characterization of protease from Bacillus Valismortis
Changes in levels of bio-chemicals and secondary metabolites during peak stre...ILRI
Presented by Ephraim Nuwamanya, Patrick R Rubaihayo, Settumba Mukasa, Samuel Kyamanywa, Robert Kawuki, Joseph Hawumba and Yona Baguma (NaCRRI/MAK) at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013
Presentation by Dr. Sarah Cianférani-Sanglier, University of Strasbourg, Strasbourg, France. Talk given at Waters Antibody Drug Conjugates (ADC) 2014 Meeting, Nov. 20-21, Wilmslow UK.
This document summarizes cluster analysis performed on sales data containing continuous variables for products like fresh, milk, and frozen goods, and nominal variables for store channel and region. Univariate analysis and scaling were performed, and two clusters were identified as the optimal number. Cluster 1 contains 135 data points and cluster 2 contains 305 points. The cluster means show differences between the two clusters for each variable.
EFFECT OF DIETARY ORGANIC SELENIUM SUPPLEMENTATION ON MASS AND ENZYME ACTIVIT...kachiwal Allah Bux
The document discusses a study that evaluated the effect of dietary organic selenium supplementation on rumen microbes and enzyme activities in goats. The study found that high concentrate diets and selenium supplementation increased short chain fatty acid concentrations and microbial populations in the rumen compared to goats on a basal diet only. While there were no significant differences between just the high concentrate diet and the high concentrate diet with selenium, rumen fermentation patterns were slightly better with selenium addition. The study concludes that selenium supplementation can improve rumen fermentation when goats are fed high concentrate diets.
The document reports on a whole genome scan that identified quantitative trait loci (QTL) associated with milk fat composition in summer milk. Four significant QTL were detected on chromosomes 14, 15, 26 and 27 that influence levels of saturated and unsaturated fatty acids. The QTL on chromosome 14 had the largest effect, influencing most fatty acid traits. Comparison to a previous study of winter milk composition found some consistent QTL on chromosomes 14 and 26, but many differences in QTL between the two seasons, likely due to environmental and design effects. The results provide opportunities for marker-assisted selection to genetically modify milk fat composition.
Stabilization of Pickering emulsion using Octenyl succinic anhydride (OSA) mo...DCSaxena1
This document summarizes research stabilizing Pickering emulsions using octenyl succinic anhydride (OSA) modified buckwheat starch. Key findings include:
- OSA modification increased buckwheat starch particle size and irregularity while decreasing amylose content. Higher OSA concentration led to greater changes.
- OSA modified buckwheat starch stabilized Pickering emulsions for 30 days with no creaming or sedimentation under high-intensity ultrasound, unlike native starch.
- Microscopic images showed OSA starch coated soybean oil droplets and prevented coalescence for 30 days. Droplet size increased slightly over time but remained nanoscale.
- OSA modification improved buckwheat starch's ability to
EPA Method 200.7, Trace Elements in Water, Solids, and Biosolids by Inductively Coupled Plasma-Atomic Emission Spectrometry, describes the procedure and requirements for multi-element determinations by ICP-AES. This presentation demonstrates the capability of the ICPE-9820, with the ASC-9800 Auto-sampler and the Standard Addition Kit, to produce quick, accurate results that comply with the method.
This monthly report summarizes Nuttapon Khongdee's work on analyzing heavy metal content, pH, EC, and organic carbon levels in different soil types before planting. Soil samples were tested for pH, EC, and organic carbon using calibrated instruments and standard procedures. Results are displayed in tables showing mean and standard deviation values for each soil type and treatment. The report also discusses initial work on characterizing citral oil nanoemulsions, including how formulation components like HLB value, homogenization speed, and oil/surfactant ratio influence particle size and stability over time. Future work is planned to analyze the nanoemulsions' antibacterial and antifungal activities.
STUDIES ON INTEGRATED BIO-HYDROGEN PRODUCTION PROCESS-EXPERIMENTAL AND MODELINGArghya_D
In the project “Studies on integrated biohydrogen production process-Experimental and Modeling”,a co-culture (mixture of two microorganisms in a single reactor) study of a dark fermentative and photofermentative microorganism was done to assess its hydrogen production performance. For modeling purpose, Artificial Neural Network and Genetic Algorithm has been used as a stochastic technique. The optimized data from batch study was successfully used to run a photobioreactor in continuous mode. A mechanistic model was developed for a continuous co-culture setup using data from literature and solved using MATLAB.
Atlantic mackerel protein hydrolysate was fractionated using electro-driven ultrafiltration (EDUF), pressure-driven ultrafiltration, and solid phase extraction to isolate immunomodulatory peptides. Fractions were tested for effects on nitric oxide production in macrophages as a measure of anti-inflammatory activity. pH 3 fractions increased nitric oxide, while the hydrophobic fraction from solid phase extraction decreased nitric oxide production, indicating anti-inflammatory activity. Amino acid analysis found the charged fractions had higher essential amino acid content than other fractions. EDUF fractions contained peptides from 2-6 amino acids in size and were identified as coming from protein precursors through mass spectrometry.
The document discusses turbidimetry and nephelometry techniques for measuring turbidity. Turbidity is caused by particles in a liquid that scatter light making the liquid appear cloudy. The intensity of turbidity depends on factors like particle quantity, size, shape, and wavelength of scattered radiation. Turbidimetry measures how light is absorbed as it passes through a sample, while nephelometry measures light scattered at an angle. Both techniques are used to analyze water quality, ions that form insoluble compounds, and microbial growth. Immunoturbidimetry uses the same principles to quantify antigens and antibodies in serum.
This document discusses calibration standards for mass spectrometers used in stable isotope analysis. It presents isotopic reference values for several calibration standards including AtlantisII, Atlantis3, ULAWATU, ESTREMOZ, BORBA, PDBBB, GoMLoP, and UCD-SM92. It also shows results from interlaboratory comparisons and discusses the need for 3-point slope corrections to improve calibration accuracy between laboratories. Suggestions are provided to standardize calibration procedures and better account for instrumental differences.
1) The document contains GDP and inflation data for various sectors in Indonesia from 2008-2018.
2) Statistical tests were performed to test the classical assumptions of the data, including normality, linearity, autocorrelation, and heteroskedasticity. The results of the tests show that the data satisfies the assumptions.
3) Multicollinearity was also tested using correlation analysis and regressing the independent variables against each other. The results show evidence of multicollinearity between some variables.
Challenges of Analyzing Petrochemicals and Organic SolventsRyan Brennan
This document discusses optimizing an ICP system for analyzing organic solvents and oils. It recommends using a low uptake nebulizer, inline particle filter, and spray chamber designed for solvents. A temperature controlled nebulizer can maintain samples from -10 to -25°C, improving volatility. A demountable torch reduces costs and a dilution probe automates oil dilution and injection into the ICP for precise analysis of wear metals.
This document characterizes polyphenol oxidase (PPO) in Ataulfo mango. It finds that PPO activity varies depending on the substrate and pH level, with optimal activity between pH 5.4-6.4. Sodium ascorbate, glutathione, and kojic acid show promise as PPO inhibitors. PPO activity is higher in mango skin than pulp. Gel electrophoresis identified a prominent PPO protein band at 53 kDaltons. The study provides information on PPO kinetics, inhibition effects, and variation between mango tissues to characterize PPO in Ataulfo mango.
Practical Implementation of the New Elemental Impurities Guidelines May 2015SGS
The International Conference on Harmonization (ICH) released its Q3D Guideline for Elemental Impurities in December 2014, initiating reviews and changes in quality testing programs in bio/pharmaceutical companies around the world. In advance of the implementation dates, companies need to assess the risks of potential elemental impurities in their process and materials streams.
In this presentation, experts will review the requirements of elemental impurities guidelines from ICH, the European Pharmacopeia, and United States Pharmacopeia, outline practical recommendations to address implementation challenges, and discuss key considerations for analytical testing programs.
Development and validation of rp hplc method for simultaneouschandu chatla
The document describes the development and validation of an RP-HPLC method for the simultaneous determination of tramadol hydrochloride, paracetamol, and dicyclomine hydrochloride in pharmaceutical formulations. The method utilizes a central composite design of experiments to optimize the chromatographic separation. Key factors investigated include mobile phase composition, pH, and flow rate. The optimized method is validated per ICH guidelines and demonstrates suitable linearity, precision, accuracy, and robustness for quality control applications.
Purification optimization and characterization of protease from Bacillus va...Vaibhav Maurya
This presentation is a research work carried out by me in B.Tech 8 semester. and gives an idea about purification, optimization and characterization of protease from Bacillus Valismortis
Similar to Clasificación de leches a través del análisis elemental por ICPMS. (20)
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...Advanced-Concepts-Team
Presentation in the Science Coffee of the Advanced Concepts Team of the European Space Agency on the 07.06.2024.
Speaker: Diego Blas (IFAE/ICREA)
Title: Gravitational wave detection with orbital motion of Moon and artificial
Abstract:
In this talk I will describe some recent ideas to find gravitational waves from supermassive black holes or of primordial origin by studying their secular effect on the orbital motion of the Moon or satellites that are laser ranged.
PPT on Sustainable Land Management presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
(June 12, 2024) Webinar: Development of PET theranostics targeting the molecu...Scintica Instrumentation
Targeting Hsp90 and its pathogen Orthologs with Tethered Inhibitors as a Diagnostic and Therapeutic Strategy for cancer and infectious diseases with Dr. Timothy Haystead.
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...Sérgio Sacani
Wereport the study of a huge optical intraday flare on 2021 November 12 at 2 a.m. UT in the blazar OJ287. In the binary black hole model, it is associated with an impact of the secondary black hole on the accretion disk of the primary. Our multifrequency observing campaign was set up to search for such a signature of the impact based on a prediction made 8 yr earlier. The first I-band results of the flare have already been reported by Kishore et al. (2024). Here we combine these data with our monitoring in the R-band. There is a big change in the R–I spectral index by 1.0 ±0.1 between the normal background and the flare, suggesting a new component of radiation. The polarization variation during the rise of the flare suggests the same. The limits on the source size place it most reasonably in the jet of the secondary BH. We then ask why we have not seen this phenomenon before. We show that OJ287 was never before observed with sufficient sensitivity on the night when the flare should have happened according to the binary model. We also study the probability that this flare is just an oversized example of intraday variability using the Krakow data set of intense monitoring between 2015 and 2023. We find that the occurrence of a flare of this size and rapidity is unlikely. In machine-readable Tables 1 and 2, we give the full orbit-linked historical light curve of OJ287 as well as the dense monitoring sample of Krakow.
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...Leonel Morgado
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
Mending Clothing to Support Sustainable Fashion_CIMaR 2024.pdfSelcen Ozturkcan
Ozturkcan, S., Berndt, A., & Angelakis, A. (2024). Mending clothing to support sustainable fashion. Presented at the 31st Annual Conference by the Consortium for International Marketing Research (CIMaR), 10-13 Jun 2024, University of Gävle, Sweden.
PPT on Direct Seeded Rice presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
Authoring a personal GPT for your research and practice: How we created the Q...Leonel Morgado
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
Candidate young stellar objects in the S-cluster: Kinematic analysis of a sub...Sérgio Sacani
Context. The observation of several L-band emission sources in the S cluster has led to a rich discussion of their nature. However, a definitive answer to the classification of the dusty objects requires an explanation for the detection of compact Doppler-shifted Brγ emission. The ionized hydrogen in combination with the observation of mid-infrared L-band continuum emission suggests that most of these sources are embedded in a dusty envelope. These embedded sources are part of the S-cluster, and their relationship to the S-stars is still under debate. To date, the question of the origin of these two populations has been vague, although all explanations favor migration processes for the individual cluster members. Aims. This work revisits the S-cluster and its dusty members orbiting the supermassive black hole SgrA* on bound Keplerian orbits from a kinematic perspective. The aim is to explore the Keplerian parameters for patterns that might imply a nonrandom distribution of the sample. Additionally, various analytical aspects are considered to address the nature of the dusty sources. Methods. Based on the photometric analysis, we estimated the individual H−K and K−L colors for the source sample and compared the results to known cluster members. The classification revealed a noticeable contrast between the S-stars and the dusty sources. To fit the flux-density distribution, we utilized the radiative transfer code HYPERION and implemented a young stellar object Class I model. We obtained the position angle from the Keplerian fit results; additionally, we analyzed the distribution of the inclinations and the longitudes of the ascending node. Results. The colors of the dusty sources suggest a stellar nature consistent with the spectral energy distribution in the near and midinfrared domains. Furthermore, the evaporation timescales of dusty and gaseous clumps in the vicinity of SgrA* are much shorter ( 2yr) than the epochs covered by the observations (≈15yr). In addition to the strong evidence for the stellar classification of the D-sources, we also find a clear disk-like pattern following the arrangements of S-stars proposed in the literature. Furthermore, we find a global intrinsic inclination for all dusty sources of 60 ± 20◦, implying a common formation process. Conclusions. The pattern of the dusty sources manifested in the distribution of the position angles, inclinations, and longitudes of the ascending node strongly suggests two different scenarios: the main-sequence stars and the dusty stellar S-cluster sources share a common formation history or migrated with a similar formation channel in the vicinity of SgrA*. Alternatively, the gravitational influence of SgrA* in combination with a massive perturber, such as a putative intermediate mass black hole in the IRS 13 cluster, forces the dusty objects and S-stars to follow a particular orbital arrangement. Key words. stars: black holes– stars: formation– Galaxy: center– galaxies: star formation
Discovery of An Apparent Red, High-Velocity Type Ia Supernova at 𝐳 = 2.9 wi...Sérgio Sacani
We present the JWST discovery of SN 2023adsy, a transient object located in a host galaxy JADES-GS
+
53.13485
−
27.82088
with a host spectroscopic redshift of
2.903
±
0.007
. The transient was identified in deep James Webb Space Telescope (JWST)/NIRCam imaging from the JWST Advanced Deep Extragalactic Survey (JADES) program. Photometric and spectroscopic followup with NIRCam and NIRSpec, respectively, confirm the redshift and yield UV-NIR light-curve, NIR color, and spectroscopic information all consistent with a Type Ia classification. Despite its classification as a likely SN Ia, SN 2023adsy is both fairly red (
�
(
�
−
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)
∼
0.9
) despite a host galaxy with low-extinction and has a high Ca II velocity (
19
,
000
±
2
,
000
km/s) compared to the general population of SNe Ia. While these characteristics are consistent with some Ca-rich SNe Ia, particularly SN 2016hnk, SN 2023adsy is intrinsically brighter than the low-
�
Ca-rich population. Although such an object is too red for any low-
�
cosmological sample, we apply a fiducial standardization approach to SN 2023adsy and find that the SN 2023adsy luminosity distance measurement is in excellent agreement (
≲
1
�
) with
Λ
CDM. Therefore unlike low-
�
Ca-rich SNe Ia, SN 2023adsy is standardizable and gives no indication that SN Ia standardized luminosities change significantly with redshift. A larger sample of distant SNe Ia is required to determine if SN Ia population characteristics at high-
�
truly diverge from their low-
�
counterparts, and to confirm that standardized luminosities nevertheless remain constant with redshift.
BIRDS DIVERSITY OF SOOTEA BISWANATH ASSAM.ppt.pptxgoluk9330
Ahota Beel, nestled in Sootea Biswanath Assam , is celebrated for its extraordinary diversity of bird species. This wetland sanctuary supports a myriad of avian residents and migrants alike. Visitors can admire the elegant flights of migratory species such as the Northern Pintail and Eurasian Wigeon, alongside resident birds including the Asian Openbill and Pheasant-tailed Jacana. With its tranquil scenery and varied habitats, Ahota Beel offers a perfect haven for birdwatchers to appreciate and study the vibrant birdlife that thrives in this natural refuge.
SDSS1335+0728: The awakening of a ∼ 106M⊙ black hole⋆Sérgio Sacani
Context. The early-type galaxy SDSS J133519.91+072807.4 (hereafter SDSS1335+0728), which had exhibited no prior optical variations during the preceding two decades, began showing significant nuclear variability in the Zwicky Transient Facility (ZTF) alert stream from December 2019 (as ZTF19acnskyy). This variability behaviour, coupled with the host-galaxy properties, suggests that SDSS1335+0728 hosts a ∼ 106M⊙ black hole (BH) that is currently in the process of ‘turning on’. Aims. We present a multi-wavelength photometric analysis and spectroscopic follow-up performed with the aim of better understanding the origin of the nuclear variations detected in SDSS1335+0728. Methods. We used archival photometry (from WISE, 2MASS, SDSS, GALEX, eROSITA) and spectroscopic data (from SDSS and LAMOST) to study the state of SDSS1335+0728 prior to December 2019, and new observations from Swift, SOAR/Goodman, VLT/X-shooter, and Keck/LRIS taken after its turn-on to characterise its current state. We analysed the variability of SDSS1335+0728 in the X-ray/UV/optical/mid-infrared range, modelled its spectral energy distribution prior to and after December 2019, and studied the evolution of its UV/optical spectra. Results. From our multi-wavelength photometric analysis, we find that: (a) since 2021, the UV flux (from Swift/UVOT observations) is four times brighter than the flux reported by GALEX in 2004; (b) since June 2022, the mid-infrared flux has risen more than two times, and the W1−W2 WISE colour has become redder; and (c) since February 2024, the source has begun showing X-ray emission. From our spectroscopic follow-up, we see that (i) the narrow emission line ratios are now consistent with a more energetic ionising continuum; (ii) broad emission lines are not detected; and (iii) the [OIII] line increased its flux ∼ 3.6 years after the first ZTF alert, which implies a relatively compact narrow-line-emitting region. Conclusions. We conclude that the variations observed in SDSS1335+0728 could be either explained by a ∼ 106M⊙ AGN that is just turning on or by an exotic tidal disruption event (TDE). If the former is true, SDSS1335+0728 is one of the strongest cases of an AGNobserved in the process of activating. If the latter were found to be the case, it would correspond to the longest and faintest TDE ever observed (or another class of still unknown nuclear transient). Future observations of SDSS1335+0728 are crucial to further understand its behaviour. Key words. galaxies: active– accretion, accretion discs– galaxies: individual: SDSS J133519.91+072807.4
Clasificación de leches a través del análisis elemental por ICPMS.
1. CLASSIFICATION OF MILK SAMPLES BASED
ON THEIR ELEMENTAL COMPOSITION.
INDUCTIVELY COUPLED PLASMA MASS
SPECTROMETRY DETERMINATION
AFTER SINGLE-STEP SOLUBILIZATION
WITH N,N-DIMETHYLFORMAMIDE.
Marianela SAVIO, Silvana M. AZCARATE,
Patricia SMICHOWSKI, José M. CAMIÑA,
Luis D. MARTINEZ, Raúl A. GIL
2. …is vital in human diet: high nutritional value and
health benefits.
…Habitual consumption is recommended.
…contain minor and trace elements, that mainly
fluctuate according to physiological functions and
extrinsic factors.
…For their nutritional and toxicological
relevance, trace element determination in
milk is of major importance.
3. … is one of the most relevant and decisive
stages in the whole analytical procedure.
The development of a simple, fast
and direct procedure for milk
analysis is thus attractive.
4. * Different percentages of:
-Water dilution (W)
-Formic acid (FA)
-Tetramethylammonium hydroxide (TMAH)
-N,N-dimethylformamide (DMF)
* Vigorous shaken
* With and without heat
6. … Another critical stage of the analytical
process is the introduction to ICPMS.
Direct analysis of milk is a challenge.
7. As, Cd, Co, Cr, Cu, Eu, Fe, Ga, Gd, Ge, K, Li, Mg, Mn, Mo, Na,
Nb, Nd, Ni, P, Pb, Pr, Rb, Si, S, Sm, Sr, Ta, Tb, Ti, V, Zn y Zr
determination was optimized
Argon Flow rate (ArFR)
Ratiofrequency Power (RF)
Sample Flow rate (SFR)
Sample introduction was
carried out using a PFA
high-efficiency
microconcentric nebulizer
coupled to a baffled
cyclonic spray chamber,
using -5°C as desolvation
temperature
8. Argon Flow rate (ArFR)
0.7 0.7
0
0.2
0.4
0.6
0.8
1
0.6 0.8 1
RelativeSignal
Argon Flow Rate (L min-1)
a.
0
5000
10000
15000
20000
25000
0.6 0.8 1
Signaltobackgrounratio
Argon Flow Rate (L min-1)
b.
As Cd
Co Cu
Eu Ga
Gd Ge
Mn Mo
Nb Nd
Ni Pb
Pr Rb
S Sm
Sr Ta
Tb V
Zn Zr
Cr, Fe, K, Li, Mg, Na, P, Si and Ti out of model
9. 0
0.2
0.4
0.6
0.8
1
1.2
700 900 1100 1300 1500
RelativeSignal
RF Power (W)
a.
0
10000
20000
30000
40000
50000
60000
70000
80000
700 900 1100 1300
Signaltobackgrounratio
RF Power (W)
b.
As Cd
Co Cu
Eu Ga
Gd Ge
Mn Mo
Nb Nd
Ni Pb
Pr Rb
S Sm
Sr Ta
Tb V
Zn Zr
Ratiofrequency Power (RF)
11. Argon Flow rate (ArFR) = 0.7 Lmin-1
Ratiofrequency Power (RF) = 1100 W
Sample Flow rate (SFR) = 0.7 mLmin-1
250 mL of milk sample
2 mL DMF (20%)
Vigorous shaken
Dilution up to 10mL
12. Microwave digestion
-0.5 g of each milk were weighed
- Treated with 7 mL of HNO3 and 1 mL of H2O2
-Subjected to a two steps temperature program
-10 min up to 200°C and up to 1000 watts
- kept 20 min at 200°C, up to 1000 watt.
- Then, samples diluted to 50 mL.
13. Mo
Nb
S
V
Zr
80%
90%
100%
110%
120%
80% 90% 100% 110% 120%
DMFrecovery(%)
MW recovery (%)
a. Aqueous Calibration
As
Cd
Co
Cu
Eu
Ga
Gd
Ge
Mn
Mo
Nb
Nd
Ni
Pb
Pr
Rb
S
Sm
Sr
Ta Tb
V
Zn
Zr
80%
90%
100%
110%
120%
80% 90% 100% 110% 120%
DMFrecovery(%)
MW recovery (%)
b. Matrix Matching
matrix matching calibration with DMF and 103Rh+ as internal standard
is recommended
14. Analite Isotope (u.m.a.)
LODa LODb RSD%
(mg L-1) (ng g-1) (20 mg L-1)
As 75 1.44 1.80 4.70
Cd 111 1.44 1.80 4.30
Co 59 1.08 1.35 6.60
Cu 63 1.04 1.30 2.90
Eu 153 0.84 1.05 3.60
Ga 69 2.64 3.30 6.20
Gd 158 0.64 0.80 1.30
Ge 74 2.48 3.10 3.30
Mn 55 1.80 2.25 2.90
Mo 98 7.72 9.65 0.50
Nb 93 0.80 1.00 1.80
Nd 142 1.16 1.45 4.80
Ni 60 4.16 5.20 7.20
Pb 208 0.80 1.00 4.60
Pr 141 1.04 1.30 2.90
Rb 85 0.92 1.15 3.40
Sm 152 0.88 1.10 2.90
Sc 48 26.20 32.75 2.40
Sr 88 1.56 1.95 1.50
Ta 181 1.04 1.30 3.70
Tb 159 0.68 0.85 1.00
V 51 1.48 1.85 2.10
Zn 66 30.76 38.45 7.40
Zr 90 0.76 0.95 5.10
aInstrumental limit of
detection;
bProcedure limit of
detection;
cDetermined as 32S16O+
15. Skim milk powder BCR 063R
Analytes
Certified values Confidence
limitsa
Experimental
values Confidence
limitsa(mg g-1) (mg g-1)
As 0.020 ±0.003
Cd <LD -
Co 0.067 ±0.028
Cu 0.602 0.019 0.600 ±0.052
Eu <LD -
Ga <LD -
Gd <LD -
Ge <LD -
Mn 0.224 ±0.008
Mo 0.287 ±0.052
Nb <LD -
Nd <LD -
Ni 1.561 ±0.296
Pb 0.0185 0.0047 0.0144 ±0.0037
Pr <LD -
Rb 17.981 ±0.837
Sm <LD -
SO 47.627 ±2.429
Sr 3.729 ±0.200
Ta <LD -
Tb <LD -
V 0.037 ±0.002
Zn 42.3 2.6 41.919 ±2.541
Zr <LD -
a
b calculated as :
100*(found – base) / added
17. Analyte A1 A2 A3 AP1 AP2 AP3 AP AW
[mg L-1] Baby 1 Baby 2 Baby 3 Baby
Premium 1
Baby
Premium 2
Baby
Premium 3
Baby
Plus 3
Whole milk
0-6 months 6-12 months 1-3 years 0-6 months 6-12 months 1-3 years 1-3 years adults
As <LD <LD 2.58±0.51 <LD 1.44±0.14 1.44±0.03 <LD 2.80±0.03
Co 2.16 ±0.17 2.74±0.31 3.88±0.57 3.94±0.93 2.92±0.01 3.76±0.34 5.58±0.54 6.90±0.36
Cu 367.1±7.6 517.7±2.5 27.1±0.7 400.8±1.3 515.5±19.4 26.58±0.48 21.22±0.31 31.64±0.39
Eu <LD <LD <LD 1.34±0.19 <LD <LD <LD <LD
Gd 0.74±0.17 <LD <LD 0.96±0.25 <LD <LD 1.18±0.01 0.9±0.1
Mn 194.2±1.47 59.8±1.5 37.1±0.2 219.3±7.8 55.0±2.4 39.12±0.28 38.94±4.21 27.86±1.89
Mo 21.14±1.13 17.8±3.6 31.5±0.1 12.38±0.79 11.6±0.02 16.38±0.84 12.18±0.51 27.6±0.31
Nb 1.74±0.42 <LD <LD <LD <LD <LD 3.40±0.39 <LD
Nd <LD <LD 1.28±0.1 2.12±0.08 <LD <LD 1.12±0.08 <LD
Ni 26.12±0.96 54.6±1.8 61.9±1.9 66.26±11.62 55.9±0.9 70.8±1.1 129.8±4.1 157.7±0.25
Pb 7.36±1.05 <LD <LD <LD 0.98±0.17 <LD <LD <LD
Rb 176.82±7.38 449.2±23.4 478.9±4.3 124.0±2.8 387.7±5.6 478.6±16.3 319.5±3.4 555.0±13.04
Sm <LD <LD <LD 1.36±0.06 <LD <LD 1.18±0.02 1.24±0.11
S 1099±51 2852±97 3159±123 1112±15 2605±5 3246±134 2498±158 3194±47
Sr 403.4±24.5 1077±42 1316±51 377.7±3.2 939±19 1343±49 1047±1 720.4±6.0
Tb <LD <LD <LD 0.84±0.14 <LD <LD 0.88±0.03 <LD
V 4.96±0.65 6.26±0.85 7.48±0.31 18.04±1.89 5.84±0.08 6.36±0.36 6.86±0.57 17.08±0.37
Zn 5295±6 5838±162 10101±61 7888±375 8569±300 10148±217 7907±227 2815±30
In all cases the concentrations of Cd, Ga, Ge, Pr, Ta and Zr were lower than their respective detection limits.
18. Analyte B1 B2 B3 BW BWRL CW CWL DW
[mg L-1]
Baby 1 Baby 2 Baby 3 Whole milk Whole milk
Lactose
Reduced
Powder
milk
Light powder milk Whole milk
0-6 months 6-12 months 1-3 years adults adults kids kids adults
As 2.86±0.45 1.82±0.22 <LD <LD 3.22±0.19 3.83±0.09 2.92±0.44 <LD
Co 5.72±0.45 7.96±0.91 7.92±0.06 7.1±0.03 5.53±0.34 4.3±0.09 5.92±0.24 5.86±0.19
Cu 391.3±10.6 573.8±20.9 219.2±4.5 31.88±3.17 25.5±0.44 18.2±0.3 29.33±0.37 25.16±0.22
Eu <LD 0.88±0.17 <LD <LD <LD <LD <LD <LD
Gd <LD <LD <LD <LD <LD <LD <LD <LD
Mn 967.4±17.5 708.1±41.4 469.0±20.5 28.8±7.4 17.07±0.49 17.3±0.1 38.23±1.62 23.26±2.91
Mo 7.60±1.50 11.48±1.44 20.16±6.64 24.0±6.9 33.50±0.08 32.16±0.12 27.6±0.8 18.1±0.9
Nb <LD <LD <LD <LD <LD <LD <LD <LD
Nd <LD <LD 1.28±0.37 <LD <LD <LD <LD <LD
Ni 54.74±5.34 123.1±7.38 153.1±11.7 162.5±28.7 121.9±6.8 101.1±2.2 131.6±5.6 141.5±7.5
Pb <LD <LD <LD <LD <LD <LD <LD <LD
Rb 387.3±13.5 354.2±22.3 602.9±22.7 691.8±72.1 697.8±14.4 433.7±0.6 497.5±10.5 437.8±8.0
Sm <LD <LD <LD <LD <LD <LD <LD <LD
S 1967±40 2260.2±90.6 2973±161 3288±413 3230±139 2374±22 3181±42 2881±132
Sr 415.6±15.0 588.2±30.5 647.9±35.6 757.2±86.8 761.5±30.1 562.4±9.7 749.3±14.8 958.7±23.8
Tb <LD <LD <LD <LD <LD <LD <LD <LD
V 9.0±0.6 12.1±1.9 11.16±0.71 13.44±3.31 11.30±0.31 11.12±0.67 8.80±0.22 10.1±0.5
Zn 5129±95 4282±132 4251±232 2981±229 2621±119 3994±68 5158±23 2293±117.7
In the case of As, Cd and Pb, they complied with the maximum limits established by the Codex Alimentarius Commission,
being <0.05; <0.05; < 0.02 mg Kg-1 respectively.
19. A1
A1
A2
A2
A3
A3
AP2
AP2
AP1
AP1
AP3
AP3
AP
AP
B1
B1
B2
B2
B3
B3
BW
BW
AW
AW
DW
DW
CW
CW
CWL
CWLBWRL
BWRL
-2000
-1500
-1000
-500
0
500
1000
1500
2000
-4000 -3000 -2000 -1000 0 1000 2000 3000 4000 5000
I II
III
IV
a.
b.
Cu
Mn
Rb
S
Sr
Zn
-0.4
-0.2
0.0
0.2
0.4
0.6
0.8
1.0
-0.20 0.00 0.20 0.40 0.60 0.80 1.00 1
a matrix of 32 x 24 was
built from the autoscaled
data, without prior signal
pretreatment
PCA model was built using
6 variables:
The first two PC’s used to build the
model, explained more than 98% of the
total variance in the data set of milk
sample analyzed
(I) Whole milks (adults)
(II) Baby milks
(III) Baby milks fortified
(premium or plus)
(IV) Milks for the first
months of life
20. Milk samples were suitably solubilized with DMF,
enabling their introduction to the ICPMS.
The proposed method …
…is fast, simple, precise, and accurate
…is attractive for routine analysis considering mainly
the multielement analysis capabilities of complex
matrices.
The quantification by external matrix matching
calibration with DMF, using 103Rh+ as internal
standard, is recommended for As, Cd, Co, Cu, Eu, Ga,
Gd, Ge, Mn, Mo, Nb, Nd, Ni, Pb, Pr, Rb, Sm, S, Sr,
Ta, Tb, V, Zn, and Zr determination.
21. PCA studies have been performed with six variables:
Zn, S, Mn, Cu, Rb and Sr.
Valuable information is obtained allowing milk
classification according to elemental contents:
adult, baby and baby fortified.
Results from this study emphasized the importance of
the multielement determination of milk samples to
carry out…
… traceability and/or origin denomination studies
… and also to assess their quality.
22.
23. Dr. Liliana Saldívar
Dr. Patricia Smichowski
Dr. Maria Goreti R. Vale
and the Organizer Committee
24. CLASSIFICATION OF MILK SAMPLES BASED
ON THEIR ELEMENTAL COMPOSITION.
INDUCTIVELY COUPLED PLASMA MASS
SPECTROMETRY DETERMINATION
AFTER SINGLE-STEP SOLUBILIZATION
WITH N,N-DIMETHYLFORMAMIDE.
Marianela SAVIOa, Silvana M. AZCARATEb,
Patricia SMICHOWSKIc,d, José M. CAMIÑAb,
Luis D. MARTINEZa, Raúl A. GILa
aInstituto de Química de San Luis (CCT-San Luis)- Área de Química Analítica. Facultad de Química Bioquímica y Farmacia. Universidad Nacional
de San Luis. Chacabuco y Pedernera. D5700BWQ San Luis, ARGENTINA.
b Instituto de Ciencias de la Tierra y Ambientales de La Pampa (INCITAP). Facultad de Ciencias Exactas y Naturales. Universidad Nacional de La
Pampa. Av. Uruguay 151. L6300XAI Santa Rosa, La Pampa, ARGENTINA.
dComisión Nacional de Energía Atómica, Gerencia Química, Av. Gral Paz 1499, B1650KNA San Martín, Buenos Aires, ARGENTINA.
eConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, C1033AAJ-Ciudad de Buenos Aires, ARGENTINA
*Author to whom correspondence should be addressed: marianelasavio@gmail.com
Editor's Notes
Milk is an important food in the human diet, as it contains many essential nutrients, providing high nutritional value and health benefits.
Habitual consumption is recommended during all stages of development and life, especially for infants and children
Milk and milk products are the most diversified natural foodstuffs, which contain minor and trace elements.
This elements mainly fluctuate according to physiological functions of cows and extrinsic factors, as human activities, environmental and geological background
For his nutritional value and toxicological relevance, trace element determination in milk is of major importance.
-----
Organic sample preparation for trace elements analysis has always been a challenge in analytical chemistry.
In fact, it is one of the most relevant and decisive stages in the whole analytical procedure for trace elements determination.
When spectrometric techniques are used for detection, a complete solubilization of sample is general required, but this procedure may cause sample losses and contamination..
So, The development of a simple, fast and direct procedure for milk analysis is attractive.
Different sample preparation were assayed
Recent contributions in this field propose treatments using:
- acid media (like formic acid) or
-alkaline media (such, tetramethylammonium hydroxide)
- Also, A simple water dilution was assayed
And In the case of the polar organic solvent dimethylformamide (DMF), we had no data with regarding to their use in preparation of organic samples; it has been used in the synthesis of organic compounds, and chromatographic applications.
The samples were Shaken vigorously
A duplicate of this samples preparation were submitted to heat
As we could observe:
With a Simple dilution, the solutions remain turbid, despite providing heat or not.
FA (formic acid) with both treatments solutions are less turbid,
Also with TMAH (Tetramethylammonium Hydroxide) solutions are less turbid. But, If heat is applied, the samples appear to undergo Maillard reactions, they change colors
In the case of using 20% DMF (dimethylformamide) and without heat, samples were appropriately solubilized, and we decide to chosee this sample preparation for the work.
We know that Inorganic mass spectrometry with inductively coupled plasma (ICPMS) is one of the most powerful analytical tools for trace elemental determination due to its extreme sensitivity, selectivity and capability of multielemental and isotopic analysis …
also is well-known that another decisive stage of the analytical process is the introduction to ICPMS through nebulization, even more if we are dealing with organic samples.
Thus, direct analysis of milk is a challenge.
Milk presents a complex colloidal system with diverse components such as fat emulsion, casein micelle suspension and others,
Usually is recommended the introduction of digested and diluted samples to ICP is required to limit the amount of dissolved solids and organic matter
So, Elemental analysis by direct nebulization of milk may block the cones, could deposit organic matter in the injector tube of the torch, and could cause spectral and non-spectral interferences due to the complexity of the matrix.
These issues may be tackled following an overall instrument optimization.
The decrease of temperature using cooled spray chamber or the addition of oxygen into the plasma that aids oxidation to organic matter, have been reported to overcome these drawbacks.
…ICPMS conditions were optimized
…
-For optimization of the instrumental parameters of ICPMS, digested samples with DMF were enriched to a final concentration of 40 ug L-1 with multielemental standard solution.
-Also a solution containing only the reagents (blank) was compared in order to evaluate the background contribution of some carbon-containing polyatomic ions.
-Sample introduction was carried out using a PFA high-efficiency microconcentric nebulizer coupled to a baffled cyclonic spray chamber, using -5°C as desolvation temperature
- The argon gas flow (Ar FR) and radiofrequency power (RF power) were optimized for maximum analyte intensity and minimum background. Also SFR was optimized.
-The decrease of temperature using cooled spray chamber or the addition of oxygen into the plasma that aids oxidation to organic matter, have been reported to overcome these drawbacks. They were also tested…we decided to work at -5C and oxigen was no nedded.
A number of 33 analites were optimized (As, Cd, Co, Cr, Cu, Eu, Fe, Ga, Gd, Ge, K, Li, Mg, Mn, Mo, Na,Nb, Nd, Ni, P, Pb, Pr, Rb, Si, S, Sm, Sr, Ta, Tb, Ti, V, Zn and Zr.)…
This was done through evaluation of the measured analytical signals in terms of ArFR (mL min-1) and RF power (watts) obtained with both synthetic sample and a reagent blank (Figs. 1 and 2).
In spite of the drawbacks mentioned above, particle sizes should not be too critical during nebulization, and samples are expected to be aspirated successfully by a pneumatic nebulizer.
In addition to particle sizes, however, the chemical form of the analyte in the matrix may also be critical
Of This (33) analites 9 were out of model.. Cr, Fe, K, Li, Mg, Na, P, Si and Ti analytes do not fit in the models and are not ilustred here.
So…24 analites were used throught analysis
We could observe in the Figure that most analytes reached maximum sensitivities at an Ar flow rate of 0.7 L min−1 and also suggests that the carbon-containing polyatomic ions did not contribute significantly; evidencing that efficient elimination of the solvent in the spray chamber was achieved.
It can be inferred that polyatomic ions containing carbon do not appear, since the signal is higher than the background, demonstrating efficient removal of the solvent in the spray chamber.
Analyte relative signals versus applied RF power was plotted, and also the contribution to background of carbon-based polyatomic ions.
The outcome indicates that despite better sensitivities were reached at 1400 W, the strong background contribution (lower SBR) moved the optimum condition to 1100 W RF power as compromise condition.
As for the carbon-based polyatomic ions: the greater RF power, the higher the formation.
The sample flow rate is a parameter directly related to the efficiency of the nebulizer, which allows controlling the entry of the sample and thus the amount of carbon.
So, sample flow rate (SFR) was optimized to reach the best nebulization efficiency and also, to handle sample (matrix) input to the ICP .
A characteristic SFR plot was thus obtained :
With increasing SFR, the signal for the analytes increased, remaining constant after 0.7 ml min-1.
Consequently, 0.7 mL min−1 SFR was thus established for all further experiments aiming to minimize the amount of carbon to be introduced to the ICP.
Final conditions for analytes determination in milk are summarizes in this tables
The samples were prepared:
using 250uL added of 2Ml of DMF shaken vigorpusly and diluted up to 10 mL
ICPMS conditions
Argon Flow rate (ArFR) = 0.7 Lmin-1
Ratiofrequency Power (RF) = 1100 W
Sample Flow rate (SFR) = 0.7 mLmin-1
Comparative studies were carried out using microwave digestion.
- 0.5 g of milk was weighed
- Was treated with 7 mL of HNO3 and 1 mL of H2O2
PTFE reactors of a microwave oven
- Was subjected to a temperature program
(ramp: 10 min at 200 ° C and up to 1000W
step of 20 min at 200 to 1000W).
Then, the samples were diluted to 50 mL.
Calibration strategy: matrix effects
With the aim of evaluate and lighten matrix effects and to come across to a proper calibration strategy for accurate analysis of milk samples treated with DMF, two approaches were evaluated:
-aqueous calibration.
- and matrix matching calibration.
Samples were prepared as indicated above, spiked with 10 ug L-1 of the analytes and the obtained % recoveries were contrasted with those obtained after microwave digestion.
- Figure a. shows that in the case of external calibration against aqueous standard solutions, only 5 analytes (Mo, Nb, S, V and Zr) could be quantitatively recovered (% recoveries between 80 and 120). If no IS was used, only As attained good recovery.
- On the other hand, Fig. b shows that when matrix matching calibration was used, quantitative recoveries of the majority of the analytes was achieve (whether IS was used or not).
Twenty three analytes out of twenty four analyzed, achieved good recoveries without IS; but when IS was used all analytes could be recovered.
In sum, it may be pointed out that matrix matching calibration with DMF and 103Rh+ as internal standard is recommended for 24 analytes (As, Cd, Co, Cu, Eu, Ga, Gd, Ge, Mn, Mo, Nb, Nd, Ni, Pb, Pr, Rb, Sm, S, Sr, Ta, Tb, V, Zn, and Zr) determination in milk samples pretreated with DMF.
The recommended procedure involved low dilution factors resulting in low detection limits (DL) for most analytes which were evaluated as 3.3 times the standard deviation (n=10) of the blank.
Detections limits varied between 0.64 ugL-1 Gd to 30.76 ugL-1 for Zn.
The precision was calculated as percent relative standard deviation (RSD %, n=3) for a standard with a concentration at the middle of the calibration plot. In all cases rsd were lower than 7.20 %
Considering the complexity of the samples analyzed, the analytical performance (Table 1) was satisfactory in comparison with figures achieved using a conventional nebulizer.
A reference material Skim milk powder (BCR 063R) from Community Bureau of Reference was employed for accuracy check.
The certified values and the found values for the analytes Cu, Pb and Zn that are present in the reference material are shown in Table
The results were compared through a paired t-test and no significant differences were observed (n=3, p=0.05).
Other analytes that were neither certified nor informed were also determined.
Spiked aliquots of this sample were also evaluated and the recoveries were quantitative.
The analyzed samples were commercially available milk samples of different brands from Argentina. A certified reference material namely,
Milk samples were labeled as: A, B, C and D according to their type and trade marks
1,2,3 according to the range of month of baby life
Each sample was independently treated with DMF as recommended and analyzed in triplicate.
The average concentrations of the analytes found in each milk sample are shown in Table 3.
The average concentrations of the analytes found in each milk sample are shown in Table 3.
In all cases the concentrations of Cd, Ga, Ge, Pr, and Ta were lower than their respective detection limits.
The presence of As, Co, Cu, Eu, Gd, Mn, Mo, Nb, Nd, Ni, Pb, Pr, Rb, S, Sm, Sr, Tb, V and Zn was evidenced in one or more milk samples.
As, Cd, Co, Cu, Eu, Ga, Gd, Ge, , Mn, Mo,,Nb, Nd, Ni, Pb, Pr, Rb, S, Sm, Sr, Ta, Tb,, V, Zn and Zr
Cr, Fe, K, Li, Mg, Na, P, Si and Ti a
The average concentrations of the analytes found in each milk sample are shown in Table 3.
In the case of As, Cd and Pb, they complied with the maximum limits established by the Codex Alimentarius Commission, being <0.05; <0.05; < 0.02 mg Kg-1 respectively.
Principal components analysis was applied to the matrix formed by the multielemental concentrations corresponding to a duplicate of the 16 milk samples. Thus, a matrix of 32 x 24 was built from the autoscaled data, without prior signal pretreatment.
From the loadings plots it was determined that the most outstanding variables in the discrimination of the groups were S, Zn, and to a lesser extent, Sr, Rb, Mn and Cu.
So, The PCA model was built using 6 variables, corresponding to Cu, Mn, Rb, S, Sr and Zn concentrations.
The first two PC’s used to build the model, explained more than 98% of the total variance in the data set of milk sample analyzed.
the score plot shows the formation of four groups:
-The samples separation through the first component is correlated with the Zn low, medium and high concentration in samples from whole milks (adults) (I), baby milks (II) and baby milks fortified (premium or plus) (III), respectively.
- There is also the formation of a subset of the second component corresponding to baby milks administered in the first few months of life (IV), which require larger amounts of Mn and Cu and less S.
It is noticeably that sample A3, unfortified milk, fell within fortified milk group.
Additionally, A3 showed an identical classification to sample AP3.
Consequently, it was possible to conclude that these samples are very similar because they belong to the same factory (perhaps the same sample in different packaging).
From this exploratory analysis it can be concluded that the content of trace metals could be related to the nutritional value required by infants at different stages of growth.
From this analysis it could be possible to evaluate a classification model that distinguish between different milks to assess fraud and / or alterations in the domestic market. This requires a study with larger sample and adding more brand names.
In this work,
A single-step sample preparation procedure is described for multi-elemental analysis of milk samples
Samples of milk are suitably solubilized with DMF, enabling their introduction to the ICPMS using a microconcentric nebulizer operated at -5 °C. and thus reducing significantly time of analysis
This undemanding and rapid sample preparation procedure makes the proposed method an attractive approach for routine analysis considering mainly the multielement analysis capabilities of complex matrices with high carbon concentration, as milk.
Furthermore, the proposed method is fast, simple, precise, accurate and less expensive than other approaches.
In addition, the achieved sensitivities are higher than those reached with conventional microwave digestion.
PCA studies have been performed with six variables: Zn, S, Mn, Cu, Rb and Sr.
Valuable information could be obtained allowing milk classification (adult, baby or baby fortified) according to elemental contents.
Results from this study emphasized the importance of the multielement determination of trace elements in milk samples to carry out traceability and/or origin denomination studies and also to assess their quality.
From this analysis could evaluate the possibility of classification models that distinguish between different milks to assess fraud and / or alterations in the domestic market. This requires a study with larger sample and adding more brand names.
A partir de este análisis se podría evaluar la posibilidad de obtener modelos de clasificación que permitan distinguir entre diferentes leches con el fin de evaluar fraudes y/o adulteraciones en el mercado nacional. Para ello es necesario un estudio con mayor número de muestras y con la incorporación de más marcas comerciales.
thank the institutions that make possible to me to be here
As the presenter expressed the title of our work is CLASSIFICATION OF MILK SAMPLES BASED ON THEIR ELEMENTAL COMPOSITION.
INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY DETERMINATION AFTER SINGLE-STEP SOLUBILIZATION WITH N,N-DIMETHYLFORMAMIDE.
This work was developed in Argentina, in national university of san luis, CONicet institute INQUISAl (chemistry institute of san luis) , in collaboration with nacional university of la pampa, CONICET institute INCITAP (earth ciences and environment institute of la pampa), and national commission of atomic energy.